Efficacy and compatibility with mass spectrometry of methods for elution of proteins from sodium dodecyl sulfate-polyacrylamide gels and polyvinyldifluoride membranes

Analytical Biochemistry

Volumn 330, Issue 1, 2004, Pages 87-97

  Jørgensen, Charlotte Sværke ^a   Jagd, Mitchell ^a   Sørensen, Birgitte Kjær ^a   McGuire, Jim ^b   Barkholt, Vibeke ^c   Højrup, Peter ^d   Houen, Gunnar ^a  

^a STATENS SERUM INSTITUT   (Denmark)

^b Symbion Research Park   (Denmark)

^c TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^d UNIVERSITY OF SOUTHERN DENMARK   (Denmark)

Author keywords

Mass spectrometry; Polyvinyldifluoride membranes; Protein elution; Sodium dodecyl sulfate polyacrylamide gels

Indexed keywords

DESORPTION; ELECTROPHORESIS; GELS; ION EXCHANGE; MAPPING; MASS SPECTROMETRY; MEMBRANES; METABOLISM; PROTEINS; SULFUR COMPOUNDS; UREA;

IDENTIFICATION OF PROTEINS; MATRIX ASSISTED LASER DESORPTION/IONIZATION; POLYACRYLAMIDE GELS; POLYVINYLDIFLUORIDE; PROTEIN CHARACTERIZATION; SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS; SURFACE-ENHANCED LASER DESORPTION/IONIZATION; TWO-DIMENSIONAL GEL ELECTROPHORESIS;

SODIUM DODECYL SULFATE;

2 PROPANOL; BUFFER; DODECYL SULFATE SODIUM; FLUORIDE; ORGANIC SOLVENT; POLYACRYLAMIDE GEL; POLYSORBATE 20; POLYVINYLDIFLUORIDE; PROTEIN; PYROXYLIN; TRIFLUOROACETIC ACID; TRITON X 100; UNCLASSIFIED DRUG; UREA;

ACETYLATION; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; ELECTROBLOTTING; ELUTION; HUMAN; HUMAN TISSUE; INTERMETHOD COMPARISON; ION EXCHANGE; MASS SPECTROMETRY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR WEIGHT; NONHUMAN; PEPTIDE MAPPING; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN ELECTROPHORESIS; PROTEIN MICROARRAY; PROTEIN TRANSPORT; SURFACE ENHANCED LASER DESORPTION IONIZATION MASS SPECTROMETRY; TANDEM MASS SPECTROMETRY;

EID: 2942576336     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2004.03.012     Document Type: Article

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