A double-emulsion microfluidic platform for in vitro green fluorescent protein expression

Journal of Micromechanics and Microengineering

Volumn 21, Issue 5, 2011, Pages

  Wu, N ^a,b   Oakeshott, J G ^a   Easton, C J ^b   Peat, T S ^a   Surjadi, R ^a   Zhu, Y ^a  

^a CSIRO   (Australia)

^b AUSTRALIAN NATIONAL UNIVERSITY   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

AQUEOUS DROPLETS; AQUEOUS PHASE; CONVENTIONAL EMULSIONS; DNA-TEMPLATE; FLUORESCEIN SODIUMS; GREEN FLUORESCENT PROTEIN; GREEN FLUORESCENT PROTEIN EXPRESSION; IN-VITRO; LASER INDUCED FLUORESCENCE; MICROFLUIDIC PLATFORMS; ON-CHIP STORAGE; PROTEIN SYNTHESIS; SYSTEM OPTIMIZATIONS; TWO-STEP PROCESS; WATER-IN-OIL; WATER-IN-OIL-IN-WATER;

BIOSYNTHESIS; DROPS; EMULSIONS; ESCHERICHIA COLI; FLUORESCENCE; MICROFLUIDICS; MICROPROCESSOR CHIPS; POLYMERASE CHAIN REACTION; PROTEINS;

EMULSIFICATION;

EID: 79955846836     PISSN: 09601317     EISSN: 13616439     Source Type: Journal    
DOI: 10.1088/0960-1317/21/5/054032     Document Type: Article

References (43)

1. Agresti J J, Antipov E, Abate A R, Ahn K, Rowat A C, Baret J-C, M M, Klibanov A M, Griffiths A D and Weitz D A 2010 Ultrahigh-throughput screening in drop-based microfluidics for directed evolution Proc. Natl Acad. Sci. 107 6550-1
2. Anna S L, Bontoux N and Stone H A 2003 Formation of dispersions using flow focusing in microchannels Appl. Phys. Lett. 82 364-6
3. Apponyi M A, Ozawa K, Dixon N E and Otting G 2008 Cell-free protein synthesis for analysis by NMR spectroscopy Methods Mol. Biol. 426 257-68
4. Bernath K, Hai M, Mastrobattista E, Griffiths A D, Magdassi S and Tawfik D S 2004 In vitro compartmentalization by double emulsions: sorting and gene enrichment by fluorescence activated cell sorting Anal. Biochem. 325 151-7 (Pubitemid 38050278)
5. Chan E M, Alivisatos A P and Mathies R A 2005 High-temperature microfluidic synthesis of CdSe nanocrystals in nanoliter droplets J. Am. Chem. Soc. 127 13854-61 (Pubitemid 41437035)
6. Discher D E, Ortiz V, Srinvias G, Klein M L, Y K, David C A, Cai S S, Photos P and Ahmed F 2007 Prog. Polym. Sci. 32 838-57
7. Dittrich P S, Jahnz M and Schwille P 2005 A new embedded process for compartmentalized cell-free protein expression and on-line detection in microfluidic devices ChemBioChem 6 811-4 (Pubitemid 40873736)
8. Garti N 1997 Double emulsions: scope, limitations and new achievements Colloids Surf. A 123 233-46 (Pubitemid 27292715)
9. Griffiths A D and Tawfik D S 2005 The laboratory in a droplet Chem. Biol. 12 1255-8
10. Guignard L, Ozawa K, Pursglove S E, Otting G and Dixon N E 2002 FEBS Lett. 524 159-62
11. Hanson J A, Chang C B, Graves S M, Li Z, Mason T G and Deming T J 2008 Nanoscale double emulsions stabilized by single-component block copolypeptides Nature 455 85-8
12. Hennequin Y, Pannacci N, de Torres C P, Tetradis-Meris G, Chapuliot S, Bouchaud E and Tabeling P 2009 Synthesizing microcapsules with controlled geometrical and mechanical properties with microfluidic double emulsion technology Langmuir 25 7857-61
13. Hong J S, Lee B S, Moon D, Lee J-G and Kang I S 2010 Pumpless dispensing of a droplet by breaking up a liquid bridge formed by electric induction Electrophoresis 31 1357-65
14. Hu S, Ren X, Bachman M, Sims C E, Li G P and Allbritton N 2002 Surface modification of poly(dimethylsiloxane) microfluidic devices by ultraviolet polymer grafting Anal. Chem. 74 4117-23 (Pubitemid 34912912)
15. Hu S, Ren X, Bachman M, Sims C E, Li G P and Allbritton N 2004 Surface-directed, graft polymerization within microfluidic channels Anal. Chem. 76 1865-70 (Pubitemid 38451501)
16. Huebner A, Olguin L F, Bratton D, Whyte G, Huck W T S, de Mello A J, Edel J B, Abell C and Hollfelder F 2008a Development of quantitative cell-based enzyme assays in microdroplets Anal. Chem. 80 3890-6 (Pubitemid 351705897)
17. Huebner A, Sharma S, Srisa-Art M, Hollfelder F, Edel J B and DeMello A J 2008b Microdroplets: a sea of applications Lab Chip 8 1244-54
18. Huebner A, Srisa-Art M, Holt D, Abell C, Hollfelder F, deMello J A and Edel J B 2007 Quantitative detection of protein expression in single cells using droplet microfluidics Chem. Commun. 1218-20 (Pubitemid 46412142)
19. Köster S et al 2008 Drop-based microfluidic devices for encapsulation of single cells Lab Chip 8 1110-5
20. Lao K-L, Wang J-H and Lee G B 2009 A microfluidic platform for formation of double-emulsion droplets Microfluid Nanofluid 7 709-19
21. Leech P W, Wu N and Zhu Y 2009 Application of dry film resist in the fabrication of microfluidic chips for droplet generation J. Micromech. Microeng. 19 1-6
22. Li M, Diehl F, Dressman D, Vogelstein B and Kinzler K W 2006 BEAMing up for detection and quantification of rare sequence variants Nat. Methods 3 95-7 (Pubitemid 43173307)
23. Mastrobattista E, Taly V, Chanudet E, Treacy P, Kelly B T and Griffiths A D 2005 High-throughput screening of enzyme libraries: in vitro evolution of a beta-galactosidase by fluorescence-activated sorting of double emulsions Chem. Biol. 12 1291-300 (Pubitemid 41779469)
24. McClements J D 1999 Food Emulsions: Principles, Practice, and Techniques (Boca Raton, FL: CRC Press)
25. Nie Z, Xu S, Seo M, Lewis P C and Kumacheva E 2005 Polymer particles with various shapes and morphologies produced in continuous microfluidic reactors J. Am. Chem. Soc. 127 8058-63 (Pubitemid 40799660)
26. Nisisako T, Torii T and Higuchi T 2002 Droplet formation in a microchannel network Lab Chip 2 24-6 (Pubitemid 37248114)
27. Okushima S, Nisisako T, Torii T and Higuchi T 2004 Controlled production of monodisperse double emulsions by two-step droplet breakup in microfluidic devices Langmuir 20 9905-8
28. Pays K, Giermanska-Kahn J, Pouligny B, Bibette J and Leal Calderon F 2002 Double emulsion: how does release occur? J. Control. Release 79 193-205 (Pubitemid 34164173)
29. Penfold R, Watson A D, Mackie A R and Hibberd D J 2006 Quantitative imaging of aggregated emulsions Langmuir 22 2005-15 (Pubitemid 43382835)
30. Reisch M S 2007 Ushering cosmetics to the right spots Chem. Eng. News 85 15-21
31. Shum H C, Kim J-W and Weitz D A 2008a Microfluidic fabrication of monodisperse biocompatible and biodegradable polymersomes with controlled permeability J. Am. Chem. Soc. 130 9543-9 (Pubitemid 352031166)
32. Shum H C, Lee D, Yoon I, Kodger T and Weitz D A 2008b Double emulsion templated monodisperse phospholipid vesicles Langmuir 24 7651-3
33. Taly V, Kelly B T and Griffiths A D 2007 Droplets as microreactors for high-throughput biology ChemBioChem 8 263-72 (Pubitemid 47194505)
34. Tawfik D S and Griffiths A D 1998 Man-made cell-like compartments for molecular evolution Nat. Biotechnol. 16 652-6 (Pubitemid 28334323)
35. Teh S Y, Lin R, Hung L H and Lee A P 2008 Droplet microfluidics Lab Chip 8 198-220 (Pubitemid 351191417)
36. Thorsen T, Roberts R W, Arnold F H and Quake S R 2001 Dynamic pattern formation in a vesicle-generating microfluidic device Phys. Rev. Lett. 86 4163-6 (Pubitemid 32443957)
37. Utada A S, Lorenceau E, Link D R, Kaplan P D, Stone H A and Weitz D A 2005 Monodisperse double emulsions generated from a microcapillary device Science 308 537-41 (Pubitemid 40570581)
38. Wu N, Zhu Y, Brown S, Oakeshott J, Peat T S, Surjadi R, Easton C, Leech P W and Sexton B A 2009 A PMMA microfluidic droplet platform for in vitro protein expression using crude E coli S30 extract Lab Chip 9 3391-8
39. Wu N, Zhu Y, Easton C, Oakeshott J and Brown S 2010 Microfluidic droplets for in vitro directed evolution Aust. J. Chem. 63 1313-25
40. Zhou C, Yue P and Feng J J 2006 Formation of simple and compound drops in microfluidic devices Phys. Fluids 18 092105
41. Zhou X et al 2004 Microfluidic PicoArray synthesis of oligodeoxynucleotides and simultaneous assembling of multiple DNA sequences Nucleic Acids Res. 32 5409-17 (Pubitemid 39545658)
42. Zhu Y, Noui-Mehidi M N, Leech P W, Sexton B A, Brown S, Wu N and Easton C 2007 Droplets transport in a microfluidic chip for in vitro compartmentalisation Proc. 16th Australian Fluid Mechanics Conf. (Gold Coast, Queensland, 3-7 December) pp 527-30
43. Zhu Y and Power B E 2008 Lab-on-a-chip in vitro compartmentalization technologies for protein studies Advances in Biochemical Engineering/ Biotechnology Protein-Protein Interaction ed T Scheper (Ser ed), vol 110, M Werther and H Seitz (Vol ed) (Berlin: Springer) pp 81-114