Development and validation of a novel SYBR Green real-time RT-PCR assay for the detection of classical swine fever virus evaluated on different real-time PCR platforms

Journal of Virological Methods

Volumn 174, Issue 1-2, 2011, Pages 53-59

  Pérez, Lester Josué ^a   Díaz de Arce, Heidy ^a   Tarradas, Joan ^b   Rosell, Rosa ^b,c   Perera, Carmen Laura ^a   Muñoz, Marta ^b   Frías, Maria T ^a   Nuñez, José Ignacio ^b   Ganges, Llilianne ^b  

^a CENTRO NACIONAL DE SANIDAD AGROPECUARIA CENSA   (Cuba)

^b UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

^c GENERALITAT DE CATALUNYA   (Spain)

Author keywords

Classical swine fever virus; Real time PCR instrument; SYBR green real time RT PCR

Indexed keywords

CYANINE DYE; NUCLEASE; SYBR GREEN DYE; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BLOOD SAMPLING; CELL CULTURE; CLASSICAL SWINE FEVER; CONTROLLED STUDY; CULTURE MEDIUM; DIAGNOSTIC TEST ACCURACY STUDY; DIAGNOSTIC VALUE; FALSE NEGATIVE RESULT; FALSE POSITIVE RESULT; GENOTYPE; LIMIT OF DETECTION; MELTING POINT; NONHUMAN; PESTIVIRUS; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RELIABILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TISSUE HOMOGENATE; VIRUS DETECTION;

ANIMALS; CLASSICAL SWINE FEVER; CLASSICAL SWINE FEVER VIRUS; CLINICAL LABORATORY TECHNIQUES; ORGANIC CHEMICALS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; STAINING AND LABELING; SWINE; VIROLOGY;

ANIMALIA; CLASSICAL SWINE FEVER VIRUS; SUIDAE;

EID: 79955785113     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2011.03.022     Document Type: Article

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