Development of SYBR green I based one-step real-time RT-PCR assay for the detection and differentiation of very virulent and classical strains of infectious bursal disease virus

Journal of Virological Methods

Volumn 161, Issue 2, 2009, Pages 271-279

  Kong, Lih Ling ^a   Omar, Abdul Rahman ^a   Hair Bejo, Mohd ^a   Ideris, Aini ^a   Tan, Sheau Wei ^a  

^a UNIVERSITY PUTRA MALAYSIA   (Malaysia)

Author keywords

Classical strain; Infectious bursal disease virus; SYBR Green 1 based real time polymerase chain reaction; Very virulent strain; VP4 gene

Indexed keywords

PROTEIN VP2;

ACCURACY; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BIRD DISEASE; CONTROLLED STUDY; DIAGNOSTIC VALUE; GENE SEQUENCE; INFECTIOUS BURSAL DISEASE; INFECTIOUS BURSAL DISEASE VIRUS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROCESS DEVELOPMENT; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SPECIES DIFFERENTIATION; VIRUS DETECTION; VIRUS ISOLATION; VIRUS STRAIN; VIRUS VIRULENCE;

ANIMALS; BIRNAVIRIDAE INFECTIONS; BURSA OF FABRICIUS; CHICKENS; INFECTIOUS BURSAL DISEASE VIRUS; ORGANIC CHEMICALS; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS, DNA; VIRAL STRUCTURAL PROTEINS;

INFECTIOUS BURSAL DISEASE VIRUS;

EID: 68949183311     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2009.06.023     Document Type: Article

References (36)

1. Bayliss C.D., Spies U., Shaw K., Peters R.W., Papageorgiou A., Muller H., and Boursnell M.G.E. A comparison of the sequences of segment A of four infectious bursal disease virus strains and identification of a variable region in VP2. J. Gen. Virol. 71 (1990) 1303-1313
2. Bengtsson M., Karlsson H.J., Westman G., and Kubista M. A new minor groove binding asymmetric cyanine reporter dye for real-time PCR. Nucleic Acids Res. 31 (2003) e45
3. Chen X., and Kwok P.Y. Homogeneous genotyping assays for single nucleotide polymorphisms with fluorescence resonance energy transfer detection. Gen. Anal. 14 (1999) 157-163
4. Chong L.K., Omar A.R., Yusoff K., Hair-Bejo M., and Aini I. Nucleotide sequence and phylogenetic analysis of A segment of a highly virulent strain of infectious bursal disease virus. Acta Virol. 45 (2001) 217-226
5. Hairul Aini H., Omar A.R., Hair-Bejo M., and Aini I. Comparison of Sybr Green I, ELISA and conventional agarose gel-based PCR in the detection of infectious bursal disease virus. Microbiol. Res. 163 (2008) 556-563
6. Heid C.A., Stevens J., Livak K.J., and Williams P.M. Real time quantitative PCR. Genome Res. 6 (1996) 986-994
7. Higuchi R., Fockler C., Dollinger G., and Watson R. Kinetic PCR analysis: real-time monitoring of DNA amplification reactions. Biotechniques (NY) 11 (1993) 1026-1030
8. Hoque M.M., Omar A.R., Chong L.K., Hair-Bejo M., and Aini I. Pathogenicity of Ssp1- positive infectious bursal disease virus and molecular characterization of the VP2 hypervariable region. Avian Pathol. 30 (2001) 369-380
9. Jackwood D.J., Saif Y.M., and Hughes J.H. Characteristics and serologic studies of two serotypes of infectious bursal disease virus in turkeys. Avian Dis. 26 (1982) 871-882
10. Jackwood D.J., and Nielsen C.K. Detection of infectious bursal disease viruses in commercially reared chickens using the reverse transcriptase/polymerase chain reaction-restriction endonuclease assay. Avian Dis. 41 (1997) 137-143
11. Jackwood D.J., Spalding B.D., and Sommer S.E. Real-time reverse transcriptase-polymerase chain reaction detection and analysis of nucleotide sequences coding for a neutralizing epitope on infectious bursal disease viruses. Avian Dis. 47 (2003) 738-744
12. Kabell S., Handberg K.J., Li Y., Kusk M., and Bisgaard M. Detection of vvIBDV in vaccinated SPF chickens. Acta Vet. Scand. 46 (2005) 219-227
13. Kong L.L., Omar A.R., Hair-Bejo M., Aini I., and Seow H.F. Comparative analysis of viral RNA and apoptotic cells in bursae following infection with infectious bursal disease virus. Comp. Immunol. Microbiol. Infect. Dis. 27 (2004) 433-442
14. Kong L.L., Omar A.R., Hair-Bejo M., Aini I., and Seow H.F. Sequence analysis of both genome segments of two very virulent infectious bursal disease virus field isolates with distinct pathogenicity. Arch. Virol. 149 (2004) 425-434
15. Le Nouen C., Rivallan G., Toquin D., Darlu P., Morin Y., Beven V., de Boisseson C., Cazaban C., Comte S., Gardin Y., and Eterradossi N. Very virulent infectious bursal disease virus: reduced pathogenicity in a rare natural segment-B reassorted isolate. J. Gen. Virol. 87 (2006) 209-216
16. Li Y.P., Hanberg K.J., Kabell S., Kusk M., Zhang M.F., and Jorgensen P.H. Relative quantification and detection of different types of infectious bursal disease virus in bursa of Fabricius and cloacal swabs using real time RT-PCR SYBR green technology. Res. Vet. Sci. 82 (2007) 126-133
17. Liu J., Zhou J., and Kwang J. Antigenic and molecular characterization of recent infectious bursal disease virus isolates in China. Virus Genes 24 (2002) 135-147
18. Lin Z., Kato A., Otaki Y., Nakamura T., Sasmaz E., and Ueda S. Sequence comparison of a highly virulent infectious bursal disease virus prevalent in Japan. Avian Dis. 37 (1993) 315-323
19. McFerran J.B., McNulty M.S., McKillop E.R., Connor T.J., McCracken R.M., Collins D.S., and Allan G.M. Isolation and serological studies with infectious bursal disease viruses from fowl, turkeys and ducks: demonstration of a second serotype. Avian Pathol. 9 (1980) 395-404
20. Monis P.T., Giglio S., and Saint C.P. Comparison of SYTO9 and SYBR Green I for real-time polymerase chain reaction and investigation of the effect of dye concentration on amplification and DNA melting curve analysis. Anal. Biochem. 340 (2005) 24-34
21. Moody A., Sellers S., and Bumstead N. Measuring infectious bursal disease virus RNA in blood by multiplex real-time quantitative RT-PCR. J. Virol. Meth. 85 (2000) 55-64
22. Muller H., Islam M.R., and Raue R. Research on infectious bursal disease-the past, the present and the future. Vet. Microbiol. 97 (2003) 153-165
23. Nurulfiza I., Hair-Bejo M., Omar A.R., and Aini I. Molecular characterization of recent infectious bursal disease virus isolates from Malaysia. Acta Virol. 50 (2006) 45-51
24. Peters M.A., Lin T.L., and Wu C.C. Real-time RT-PCR differentiation and quantitation of infectious bursal disease virus strains using dual-labeled fluorescent probes. J. Virol. Meth. 127 (2005) 87-95
25. Piatek A.S., Tyagi S., Pol A.C., Telenti A., Miller L.P., Kramer D., and Alland D. Molecular beacon sequence analysis for detection drug resistance in Mycobacterium tuberculosis. Nat. Biotechnol. 16 (1998) 359-363
26. Ririe K.M., Rasmussen R.P., and Wittwer C.T. Product differentiation by analysis of DNA melting curve during the polymerase chain reaction. Anal. Biochem. 245 (1997) 54-160
27. Rudd M.F., Heine H.G., Sapats S.I., Parede L., and Ignjatovic J. Characterization of an Indonesian very virulent strain of infectious bursal disease virus. Arch. Virol. 147 (2002) 1303-1322
28. Solinas A., Brown L.J., McKeen C., Mellor J.M., Nicol J., Thelwell N., and Brown T. Duplex scorpion primers in SNP analysis and FRET application. Nucleic Acids Res. 29 (2001) E96
29. Sreedevi B., and Jackwood D.J. Real-time reverse transcriptase-polymerase chain reaction detection and sequence analysis of the hypervariable region of Indian very virulent infectious bursal disease virus isolated. Avian Dis. 51 (2007) 750-757
30. Tham K.M., Young L.W., and Moon C.D. Detection of infectious bursal disease virus by reverse transcription-polymerase chain reaction amplification of the segment A gene. J. Virol. Meth. 53 (1995) 201-212
31. Wei Y., Yu X., Zheng J., Chu W., Xu H., Yu X., and Yu L. Reassortant infectious bursal disease virus isolated in China. Virus Res. 131 (2008) 279-282
32. Wittwer C.T., Reed G.H., Gundry C.N., Vandersteen J.G., and Pryor R.J. High-resolution genotyping by amplicon melting analysis using LC Green. Clin. Chem. 49 (2003) 853-860
33. Wu C.C., Lin T.L., and Akin A. Quantitative comparative polymerase chain reaction for detection and quantitation of infectious bursal disease virus cDNA and RNA. J. Virol. Meth. 66 (1997) 29-38
34. Wu C.C., Rubinelli P., and Lin T.L. Molecular detection and differentiation of infectious bursal disease virus. Avian Dis. 51 (2007) 515-526
35. Xia R.X., Wang H.W., Huang G.M., and Zhang M.F. Sequence and phylogenetic analysis of a Chinese very virulent infectious bursal disease virus. Arch. Virol. 153 (2008) 1725-1729
36. Yamaguchi T., Kasanga C.J., Terasaki K., Maw M.T., Ohya K., and Fukushi H. Nucleotide sequence analysis of VP2 hypervariable domain of infectious bursal disease virus detected in Japan from 1993 to 2004. J. Vet. Med. Sci. 69 (2007) 733-738