A rapid and simple procedure for the establishment of human normal and cancer renal primary cell cultures from surgical specimens

PLoS ONE

Volumn 6, Issue 5, 2011, Pages

  Valente, Maria João ^a   Henrique, Rui ^a,b   Costa, Vera L ^b   Jerónimo, Carmen ^a,b   Carvalho, Félix ^a   Bastos, Maria L ^a   de Pinho, Paula Guedes ^a   Carvalho, Márcia ^a,c  

^a UNIVERSITY OF PORTO   (Portugal)

^b PORTUGUESE ONCOLOGY INSTITUTE   (Portugal)

^c UNIVERSIDADE FERNANDO PESSOA   (Portugal)

Author keywords

[No Author keywords available]

Indexed keywords

AQUAPORIN 3; BIOLOGICAL MARKER; CYTOKERATIN; GLUTAMYL AMINOPEPTIDASE; TAMM HORSFALL GLYCOPROTEIN;

ADULT; AGED; ARTICLE; CANCER CELL CULTURE; CANCER MORPHOLOGY; CELL ISOLATION; CELL STRUCTURE; CELL VIABILITY; CLINICAL PROTOCOL; CONTROLLED STUDY; ENZYMATIC DEGRADATION; FEMALE; HISTOPATHOLOGY; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOFLUORESCENCE TEST; IN VITRO STUDY; KIDNEY CARCINOMA; KIDNEY CELL CULTURE; LABORATORY DIAGNOSIS; MALE; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STRUCTURE ANALYSIS; TISSUE SECTION; CULTURE TECHNIQUE; CYTOLOGY; KIDNEY; KIDNEY TUMOR; METHODOLOGY; MIDDLE AGED; PATHOLOGY; TUMOR CELL CULTURE;

AGED; CELL CULTURE TECHNIQUES; FEMALE; HUMANS; KIDNEY; KIDNEY NEOPLASMS; MALE; MIDDLE AGED; TUMOR CELLS, CULTURED;

EID: 79955761537     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0019337     Document Type: Article

References (35)

1. Carvalho M, Hawksworth G, Milhazes N, Borges F, Monks TJ, et al. (2002) Role of metabolites in MDMA (ecstasy)-induced nephrotoxicity: an in vitro study using rat and human renal proximal tubular cells. Arch Toxicol 76: 581-588.
2. Kuwana H, Terada Y, Kobayashi T, Okado T, Penninger JM, et al. (2008) The phosphoinositide-3 kinase gamma-Akt pathway mediates renal tubular injury in cisplatin nephrotoxicity. Kidney Int 73: 430-445.
3. Lash LH, Putt DA, Hueni SE, Payton SG, Zwickl J, (2007) Interactive toxicity of inorganic mercury and trichloroethylene in rat and human proximal tubules: effects on apoptosis, necrosis, and glutathione status. Toxicol Appl Pharmacol 221: 349-362.
4. Schwerdt G, Holzinger H, Sauvant C, Konigs M, Humpf HU, et al. (2007) Long-term effects of ochratoxin A on fibrosis and cell death in human proximal tubule or fibroblast cells in primary culture. Toxicology 232: 57-67.
5. Tune BM, Fravert D, (1980) Mechanisms of cephalosporin nephrotoxicity: a comparison of cephaloridine and cephaloglycin. Kidney Int 18: 591-600.
6. Hawksworth GM, (2005) Isolation and culture of human renal cortical cells with characteristics of proximal tubules. Methods Mol Med 107: 283-290.
7. Kruidering M, Van de Water B, de Heer E, Mulder GJ, Nagelkerke JF, (1997) Cisplatin-induced nephrotoxicity in porcine proximal tubular cells: mitochondrial dysfunction by inhibition of complexes I to IV of the respiratory chain. J Pharmacol Exp Ther 280: 638-649.
8. Schaaf GJ, de Groene EM, Maas RF, Commandeur JN, Fink-Gremmels J, (2001) Characterization of biotransformation enzyme activities in primary rat proximal tubular cells. Chem Biol Interact 134: 167-190.
9. Vesey DA, Qi W, Chen X, Pollock CA, Johnson DW, (2009) Isolation and primary culture of human proximal tubule cells. Methods Mol Biol 466: 19-24.
10. McAteer JA, Kempson SA, Evan AP, (1991) Culture of human renal cortex epithelial cells. J Tissue Cult Methods 13: 143-148.
11. Wilson PD, Dillingham MA, Breckon R, Anderson RJ, (1985) Defined human renal tubular epithelia in culture: growth, characterization, and hormonal response. Am J Physiol 248: F436-443.
12. Kim FJ, Campagna A, Khandrika L, Koul S, Byun SS, et al. (2008) Individualized medicine for renal cell carcinoma: establishment of primary cell line culture from surgical specimens. J Endourol 22: 2361-2366.
13. Jemal A, Siegel R, Xu J, Ward E, (2010) Cancer statistics, 2010. CA Cancer J Clin 60: 277-300.
14. Park JG, Ku JL, Park SY, (2004) Isolation and culture of renal cancer cell lines. Methods Mol Med 88: 111-119.
15. Pittoggi C, Martis G, Mastrangeli G, Mastrangeli B, Spadafora C, (2008) In vitro evidence for a new therapeutic approach in renal cell carcinoma. Int Braz J Urol 34: 492-502.
16. Zbar B, Glenn G, Lubensky I, Choyke P, Walther MM, et al. (1995) Hereditary papillary renal cell carcinoma: clinical studies in 10 families. J Urol 153: 907-912.
17. Yang XJ, Tan MH, Kim HL, Ditlev JA, Betten MW, et al. (2005) A molecular classification of papillary renal cell carcinoma. Cancer Res 65: 5628-5637.
18. Liu L, Qian J, Singh H, Meiers I, Zhou X, et al. (2007) Immunohistochemical analysis of chromophobe renal cell carcinoma, renal oncocytoma, and clear cell carcinoma: an optimal and practical panel for differential diagnosis. Arch Pathol Lab Med 131: 1290-1297.
19. Yusenko MV, (2010) Molecular pathology of chromophobe renal cell carcinoma: a review. Int J Urol 17: 592-600.
20. Launonen V, Vierimaa O, Kiuru M, Isola J, Roth S, et al. (2001) Inherited susceptibility to uterine leiomyomas and renal cell cancer. Proc Natl Acad Sci U S A 98: 3387-3392.
21. Mizutani Y, Wada H, Yoshida O, Fukushima M, Nonomura M, et al. (2003) Significance of thymidylate synthase activity in renal cell carcinoma. Clin Cancer Res 9: 1453-1460.
22. Wu XX, Mizutani Y, Kakehi Y, Yoshida O, Ogawa O, (2000) Enhancement of Fas-mediated apoptosis in renal cell carcinoma cells by adriamycin. Cancer Res 60: 2912-2918.
23. Valente MJ, Baltazar AF, Henrique R, Estevinho L, Carvalho M, (2011) Biological activities of Portuguese propolis: Protection against free radical-induced erythrocyte damage and inhibition of human renal cancer cell growth in vitro. Food Chem Toxicol 49: 86-92.
24. Glynne P, (2000) Primary culture of human proximal renal tubular epithelial cells. Methods Mol Med 36: 197-205.
25. Qi W, Johnson DW, Vesey DA, Pollock CA, Chen X, (2007) Isolation, propagation and characterization of primary tubule cell culture from human kidney. Nephrology (Carlton) 12: 155-159.
26. Ebert T, Bander NH, Finstad CL, Ramsawak RD, Old LJ, (1990) Establishment and characterization of human renal cancer and normal kidney cell lines. Cancer Res 50: 5531-5536.
27. Price KL, Hulton SA, van't Hoff WG, Masters JR, Rumsby G, (2009) Primary cultures of renal proximal tubule cells derived from individuals with primary hyperoxaluria. Urol Res 37: 127-132.
28. Bens M, Vandewalle A, (2008) Cell models for studying renal physiology. Pflugers Arch 457: 1-15.
29. Pfaller W, Gstraunthaler G, (1998) Nephrotoxicity testing in vitro--what we know and what we need to know. Environ Health Perspect 106 (Suppl 2): 559-569.
30. Baer PC, Nockher WA, Haase W, Scherberich JE, (1997) Isolation of proximal and distal tubule cells from human kidney by immunomagnetic separation. Technical note. Kidney Int 52: 1321-1331.
31. Johnson DW, Brew BK, Poronnik P, Cook DI, Gyory AZ, et al. (1997) Transport characteristics of human proximal tubule cells in primary culture. Nephrology, 3: 183-194.
32. Terryn S, Jouret F, Vandenabeele F, Smolders I, Moreels M, et al. (2007) A primary culture of mouse proximal tubular cells, established on collagen-coated membranes. Am J Physiol Renal Physiol 293: F476-485.
33. Bodmer D, van den Hurk W, van Groningen JJ, Eleveld MJ, Martens GJ, et al. (2002) Understanding familial and non-familial renal cell cancer. Hum Mol Genet 11: 2489-2498.
34. Anglard P, Trahan E, Liu S, Latif F, Merino MJ, et al. (1992) Molecular and cellular characterization of human renal cell carcinoma cell lines. Cancer Res 52: 348-356.
35. Gerharz CD, Moll R, Storkel S, Ramp U, Thoenes W, et al. (1993) Ultrastructural appearance and cytoskeletal architecture of the clear, chromophilic, and chromophobe types of human renal cell carcinoma in vitro. Am J Pathol 142: 851-859.