Ion mobility mass spectrometry for extracting spectra of n-glycans directly from incubation mixtures following glycan release: Application to glycans from engineered glycoforms of intact, folded HIV gp120

Journal of the American Society for Mass Spectrometry

Volumn 22, Issue 3, 2011, Pages 568-581

  Harvey, David J ^a   Sobott, Frank ^c   Crispin, Max ^a   Wrobel, Antoni ^a   Bonomelli, Camille ^a   Vasiljevic, Snezana ^a   Scanlan, Christopher N ^a   Scarff, Charlotte A ^b   Thalassinos, Konstantinos ^b,d   Scrivens, James H ^b  

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b UNIVERSITY OF WARWICK   (United Kingdom)

^c UNIVERSITY OF ANTWERP   (Belgium)

^d UNIVERSITY OF LONDON   (United Kingdom)

Author keywords

Glycan release; Gp120 glycoprotein; Ion mobility; N glycans; Negative ion fragmentation

Indexed keywords

GLYCAN RELEASE; GP120 GLYCOPROTEIN; ION MOBILITY; N-GLYCANS; NEGATIVE ION FRAGMENTATION;

BIOLOGICAL MATERIALS; CELL CULTURE; ELECTROSPRAY IONIZATION; GLYCOPROTEINS; MAMMALS; MASS SPECTROMETRY; NEGATIVE IONS; SOAPS (DETERGENTS); STRUCTURAL ANALYSIS; SURFACE ACTIVE AGENTS; VIRUSES;

DISEASES;

DETERGENT; GLYCAN DERIVATIVE; GLYCOPEPTIDASE; GLYCOPROTEIN GP 120; MANNOSIDASE INHIBITOR;

ARTICLE; CARBOHYDRATE ANALYSIS; CONTROLLED STUDY; ELECTROSPRAY MASS SPECTROMETRY; EMBRYO; FRAGMENTATION REACTION; GLYCOSYLATION; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS; INCUBATION TIME; ION MOBILITY MASS SPECTROMETRY; ION MOBILITY SPECTROMETRY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN FOLDING; SEPARATION TECHNIQUE; SIGNAL NOISE RATIO; STRUCTURE ANALYSIS; TANDEM MASS SPECTROMETRY; TITRIMETRY; VIRUS ENVELOPE;

GLYCOPROTEINS; HEK293 CELLS; HIV ENVELOPE PROTEIN GP120; HUMANS; MASS SPECTROMETRY; N-ACETYLGLUCOSAMINYLTRANSFERASES; PEPTIDE-N4-(N-ACETYL-BETA-GLUCOSAMINYL) ASPARAGINE AMIDASE; POLYSACCHARIDES; PROTEIN ENGINEERING; RECOMBINANT PROTEINS;

HUMAN IMMUNODEFICIENCY VIRUS; MAMMALIA;

EID: 79955753022     PISSN: 10440305     EISSN: 18791123     Source Type: Journal    
DOI: 10.1007/s13361-010-0053-0     Document Type: Article

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