N- and O-linked carbohydrates and glycosylation site occupancy in recombinant human granulocyte-macrophage colony-stimulating factor secreted by a Chinese hamster ovary cell line

European Journal of Biochemistry

Volumn 271, Issue 5, 2004, Pages 907-919

  Forno, Guillermina ^a   Bollati Fogolin, Mariela ^a   Oggero, Marcos ^a   Kratje, Ricardo ^a   Etcheverrigaray, Marina ^a   Conradt, Harald S ^b   Nimtz, Manfred ^b  

^a UNIVERSIDAD NACIONAL DEL LITORAL   (Argentina)

^b HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

Author keywords

Glycoprotein; Intact protein MALDI TOF; N linked oligosaccharides; Recombinant human granulocyte macrophage colony stimulating factor; Site specific O glycosylation

Indexed keywords

CARBOHYDRATE DERIVATIVE; GALACTOSE; GLYCAN DERIVATIVE; GLYCOPROTEIN; HEXOSE; LACTOSE; N ACETYLNEURAMINIC ACID; N GLYCOLOYLNEURAMINIC ACID; OLIGOSACCHARIDE; POLYPEPTIDE; RECOMBINANT GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR; SERINE; THREONINE;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ANIMAL CELL CULTURE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL GROWTH; CHO CELL; CONTROLLED STUDY; CYTOKINE RELEASE; ELECTROSPRAY MASS SPECTROMETRY; GLYCOSYLATION; HUMAN; ISOELECTRIC FOCUSING; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR WEIGHT; NONHUMAN; OVARY CELL; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; SIALYLATION;

AMINO ACID SEQUENCE; ANIMALS; CARBOHYDRATE CONFORMATION; CARBOHYDRATE METABOLISM; CARBOHYDRATE SEQUENCE; CARBOHYDRATES; CHO CELLS; CRICETINAE; GLYCOSYLATION; GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR; HUMANS; MOLECULAR SEQUENCE DATA; PEPTIDES; PROTEIN ISOFORMS; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECOMBINANT PROTEINS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

ANIMALIA; CRICETINAE; CRICETULUS GRISEUS;

EID: 1542314755     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.2004.03993.x     Document Type: Article

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