Sensitivity of Allium and Nicotiana in cellular and acellular comet assays to assess differential genotoxicity of direct and indirect acting mutagens

Ecotoxicology and Environmental Safety

Volumn 74, Issue 4, 2011, Pages 860-865

  Bandyopadhyay, Atrayee ^a   Mukherjee, Anita ^a  

^a UNIVERSITY OF CALCUTTA   (India)

Author keywords

Acellular; Allium; Comet assay; Genotoxicity; Nicotiana

Indexed keywords

CADMIUM CHLORIDE; CATALASE; HYDROGEN PEROXIDE; MESYLIC ACID ETHYL ESTER; MUTAGENIC AGENT; PLANT DNA;

DNA; DOSE-RESPONSE RELATIONSHIP; GENOTOXICITY; MUTAGENICITY; ROOT-SHOOT RATIO; SENSITIVITY ANALYSIS; SHOOT GROWTH; TOBACCO; VEGETABLE;

ARTICLE; CELL ISOLATION; CELL MEMBRANE; COMET ASSAY; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DNA DAMAGE; ENVIRONMENTAL IMPACT ASSESSMENT; ENVIRONMENTAL MONITORING; GENOTOXICITY; NONHUMAN; ONION; PLANT CELL; PLANT LEAF; PLANT RESPONSE; PLANT ROOT; PLANT STRESS; SENSITIVITY ANALYSIS; SHOOT; TOBACCO; TOXICITY TESTING;

CADMIUM CHLORIDE; CELL NUCLEUS; COMET ASSAY; DNA DAMAGE; DOSE-RESPONSE RELATIONSHIP, DRUG; ETHYL METHANESULFONATE; HYDROGEN PEROXIDE; MUTAGENS; ONIONS; PLANT ROOTS; SOIL POLLUTANTS; TOBACCO;

ALLIUM; ALLIUM CEPA; NICOTIANA; NICOTIANA TABACUM;

EID: 79955521879     PISSN: 01476513     EISSN: 10902414     Source Type: Journal    
DOI: 10.1016/j.ecoenv.2010.12.002     Document Type: Article

References (42)

1. Badisa V.L., Latinwo L.M., Odewumi C.O., Ikediobi C.O., Badisa R.B., Ayuk-Takem L.T., Nwoga J., West J. Mechanism of DNA damage by cadmium and interplay of antioxidant enzymes and agents. Environ. Toxicol. 2007, 22:144-151.
2. Chakraborty R., Mukherjee A.K., Mukherjee A. Evaluation of genotoxicity of coal fly ash in Allium cepa root cells by combining comet assay with the Allium test. Environ. Monit. Assess 2009, 153:351-356.
3. Collins A.R., Dušinská M., Gedik C.M., Šťetina R. Oxidative damage to DNA: do we have a reliable biomarker?. Environ. Health Perspect. 1996, 104:465-469.
4. Collins A.R., Dobson V.L., Dušinská M., Kennedy G., Šťetina R. The comet assay: what it really tell us?. Mutat. Res. 1997, 375:183-193.
5. Collins A.R. The comet assay for DNA damage and repair: principles, applications, and limitations. Molec. Biotechnol. 2004, 26:249-261.
6. Dat J.F., Inzé D., Van Breusegem F. Catalase-deficient tobacco plants: tools, for in planta studies for the role of hydrogen peroxide. Redox Report 2001, 6:37-42.
7. Gichner T., Plewa M.J. Induction of somatic DNA damage as measured by single cell gel electrophoresis and point mutation in leaves of tobacco plants. Mutat. Res. 1998, 401:143-152.
8. Gichner T. DNA damage induced by indirect and direct acting mutagens in catalase-deficient transgenic tobacco cellular and acellular comet assay. Mutat. Res. 2003, 535:187-193.
9. Gichner T. Differential genotoxicity of ethyl methanesulphonate, N-ethyl-N nitrosourea and maleic hydrazide in tobacco seedlings based on data of the comet assay and two recombination assays. Mutat. Res. 2003, 538:171-179.
10. Gichner T., Patková Z., Száková J., Demnerová K. Cadmium induces DNA damage in tobacco roots, but no DNA damage, somatic mutations or homologous recombination in tobacco leaves. Mutat. Res. 2004, 559:49-57.
11. Gichner T., Lovecká P., Kochánková L., Macková M., Demnerová K. Monitoring toxicity, DNA damage, and somatic mutations in tobacco plants growing in soil heavily polluted with polychlorinated biphenyls. Mutat. Res. 2007, 629:1-6.
12. Gichner T., Patková Z., Száková J., Znidar I., Mukherjee A. DNA damage in potato plants induced by cadmium, ethyl methanesulphonate and γ rays. Environ. Exp. Bot. 2008, 62:113-119.
13. Giri A.K., Roychoudhury A. Effects of certain food dyes on chromosomes of Allium cepa. Mutat. Res. 1989, 223:313-319.
14. Grant W.F. The present status of higher plant bioassays for detection of environmental mutagens. Mutat. Res. 1994, 310:175-185.
15. Hattab S., Chouba L., Ben Kheder M., Mahouachi T., Boussetta H. Cadmium- and copper-induced DNA damage in Pisum sativum roots and leaves as determined by the comet assay. Plant Biosystems 2009, 143:S6-11.
16. Heflich R.H. Chemical mutagens. Genetic Toxicology 1991, 143-202. CRC Press, Florida. A.P. Li, R.H. Heflich (Eds.).
17. Jovtchev G., Menke M., Schubert I. The comet assay detects adaptation to MNU-induced DNA damage in barley. Mutat. Res. 2001, 493:95-100.
18. Juchimuik J., Maluszynska J. Transformed roots of Crepis capillaris-a sensitive system for the evaluation of the clastogenicity of abiotic agents. Mutat. Res. 2005, 565:129-138.
19. Juchimiuk J., Gnys A., Maluszynska J. DNA damage induced by mutagens in plant and human cell nuclei in acellular comet assay. Folia Histochem. Cytobiol. 2006, 44:127-131.
20. Kasamatsu T., Kohda K., Kawazoe Y. Comparison of chemically induced DNA breakage in cellular and subcellular systems using the comet assay. Mutat. Res. 1996, 369:1-6.
21. Koppen G., Verschaeve L. The alkaline comet test on plant cells: a new genotoxicity test for DNA strand breaks in Vicia faba root cells. Mutat. Res. 1996, 360:193-200.
22. Kovalchuk I., Kovalchuk O. Canada transgenic plants as sensors of environmental pollution genotoxicity. Sensors 2008, 8:1539-1558.
23. Leme D.M., Marin-Morales M.A. Chromosome aberration and micronucleus frequencies in Allium cepa cells exposed to petroleum polluted water-a case study. Mutat. Res. 2008, 650:80-86.
24. Leme D.M., Marin-Morales M.A. Allium cepa test in environmental monitoring: a review on its application. Mutat. Res. 2009, 682:71-81.
25. Lovell D.P., Tomas G., Dulbow R. Issues related to the experimental design and subsequent statistical analysis of in vivo and in vitro comet studies. Teratog. Carcinog. Mutagen 1999, 19:109-119.
26. Matsumoto S.T., Dias A.L., Mantovani M.S., Ariza Malaguttii M.I., Fonseca I.C., Marin-Morales M.A. Genotoxicity and mutagenicity of water contaminated with tannery effluents, as evaluated by the micronucleus test and comet assay using the fish Oreochromis niloticus and chromosome aberrations in onion root-tips. Genet. Mol. Biol. 2006, 29:148-158.
27. Menke M., Peng Chen I., Angelis K.J., Schubert I. DNA damage and repair in Arabidopsis thaliana as measured by the comet assay after treatment with different classes of genotoxins. Mutat. Res. 2001, 493:87-93.
28. Mishra K., Srivastava K. Cytogenetic effects of commercially formulated atrazine on the somatic cells of Allium cepa and Vicia faba. Pestic. Biochem. Physiol. 2009, 93:8-12.
29. Mukherjee, A., Gichner, T., 2009. Plant bioassays: comet assay on higher plants. In: Sampietro, D., Vattuone, M.A., Catalan, C.A.N., Politycka, B. (Eds.), Studium Press, LLC, Texas, USA, pp. 97-108.
30. Navarrete M.H., Carrera P., De Miguel M., De la Torre C. A fast comet assay variant for solid tissue cells. The assessment of DNA damage in higher plants. Mutat. Res. 1997, 389:271-277.
31. Panda K.K., Lenka M., Panda B.B. Monitoring and assessment of mercury pollution in the vicinity of a chloralkali plant. 1. Distribution, availability and genotoxicity of sediment mercury in the Rushikulya estuary. India Sci. Total Environ. 1990, 96:281-296.
32. Panda B.B., Panda K.K. Genotoxicity and mutagenicity of metals in plants. Physiology and Biochemistry of Metal Toxicity and Tolerance in Plants 2002, 395-414. Kluwer Academic Publishers, The Netherlands, (Chapter 15). K.N.V. Prasad, K. Strzalka (Eds.).
33. Saghirzadeh M., Gharaatib M.R., Mohammadic Sh., Ghiassi-Nejad M. Evaluation of DNA damage in the root cells of Allium cepa seeds growing in soil of high background radiation areas of Ramsar-Iran. J. Environ. Radioact. 2008, 99:1698-1702.
34. Sanita di Toppi L., Gabbrieli R. Responses to cadmium in higher plants. Environ. Exp. Bot. 1999, 41:105-130.
35. Stavreva D.A., Gichner T. DNA damage induced by hydrogen peroxide in cultured tobacco cells is dependent on the cell growth stage. Mutat. Res. 2002, 514:147-152.
36. Steinkellner H., Mun-Sik K., Helma C., Ecker S., Ma T.H., Horak O., Kundi M., Knasmüller S. Genotoxic effects of heavy metals: comparative investigation with plant bioassays. Environ. Mol. Mutag. 1998, 31:183-191.
37. Szeto Y.T., Collins A.R., Benzie I.F. Effects of dietary antioxidants on DNA damage in lysed cells using a modified comet assay procedure. Mutat. Res. 2002, 500:31-38.
38. Termini J. Hydroperoxide-induced DNA damage and mutations. Mutat. Res. 2000, 450:107-124.
39. Thomas S., Lowe J.E., Knowles R.G., Green I.C., Green M.H.L. Factors affecting the DNA damaging activity of superoxide and nitric oxide. Mutat. Res. 1998, 402:77-84.
40. Tice R.R., Agurell E., Anderson D., Burlinson B., Hartmann A., Kobayashi H., Miyamae Y., Rojas E., Ryu J.C., Sasaki Y.F. Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ. Mol. Mutagen. 2000, 35:206-221.
41. Valverde M., Trejo C., Rojas E. Is the capacity of lead acetate and cadmium chloride to induce genotoxic damage due to direct DNA-metal interaction?. Mutagenesis 2001, 16:265-270.
42. Vasquez M., Tice R.R. Detecting genotoxic activity against high molecular weight DNA using the alkaline single cell gel (SCG) assay. Environ. Mol. Mutagen. 1997, 29:28-53.