Induction of somatic DNA damage as measured by single cell gel electrophoresis and point mutation in leaves of tobacco plants

Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

Volumn 401, Issue 1-2, 1998, Pages 143-152

  Gichner, Tomáš ^a   Plewa, Michael J ^b,c  

^a INSTITUTE OF EXPERIMENTAL BOTANY   (Czech Republic)

^b UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^c MC 635 ^*  (United States)

Author keywords

Comet assay; Ethyl methanesulfonate; Nicotiana tabacum var. xanthi; Plant mutation assay; SCG electrophoresis

Indexed keywords

DNA; MESYLIC ACID ETHYL ESTER;

ARTICLE; CONTROLLED STUDY; DNA DAMAGE; ENVIRONMENTAL IMPACT ASSESSMENT; GEL ELECTROPHORESIS; MUTATION RATE; NONHUMAN; PLANT LEAF; POINT MUTATION; PRIORITY JOURNAL; TOBACCO;

DNA DAMAGE; DNA, PLANT; ELECTROPHORESIS, AGAR GEL; ETHYL METHANESULFONATE; MUTAGENICITY TESTS; MUTAGENS; PLANT LEAVES; PLANTS, TOXIC; POINT MUTATION; TOBACCO;

NICOTIANA OBTUSIFOLIA; NICOTIANA TABACUM; TABACUM;

EID: 0032486087     PISSN: 00275107     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0027-5107(98)00003-7     Document Type: Article

References (19)

1. D.W. Fairbairn, P.L. Olive, K.L. O'Neill, The comet assay: a comprehensive review, Mutation Res. 339 (1995) 37-59.
2. R.R. Tice, The single cell gel/comet assay: a microgel electrophoretic technique for the detection of DNA damage and repair in individual cells, in: D.H. Phillips, S. Venitt (Eds.), Environmental Mutagenesis, Bios Scientific Publishers, Oxford, 1995, pp. 315-339.
3. N.P. Singh, T. McCoy, R.R. Tice, E.L. Schneider, A simple technique for quantitation of low levels of DNA damage in individual cells, Exp. Cell Res. 175 (1988) 84-192.
4. P. Fortini, G. Raspaglio, M. Falchi, E. Dogliotti, Analysis of DNA alkylation damage and repair in mammalian cells by the comet assay, Mutagenesis 11 (1996) 169-175.
5. G. Koppen, L. Verschaeve, The alkaline comet test on plant cells: a new genotoxicity test for DNA strand breaks in Vicia faba root cells, Mutation Res. 360 (1996) 193-200.
6. M.H. Navarrete, P. Carrera, M. de Miguel, C. de la Torre, A fast cornet assay variant for solid tissue cells. The assessment of DNA damage in higher plants, Mutation Res. 389 (1997) 271-277.
7. M.J. Constantin, E.T. Owens, Introduction and perspectives of plant genetic and cytogenetic assays - a report of the U.S. Environmental Protection Agency Gene-Tox Program, Mutation Res. 99 (1982) 1-12.
8. W.F. Grant, The present status of higher plant bioassays for the detection of environmental mutagens, Mutation Res. 310 (1994) 175-185.
9. H.L. Dulieu, M.A. Dalebroux, Spontaneous and induced reversion rates in a double heterozygous mutant of Nicotiana tabacum var. xanthi n.c.: dose-response relationship, Mutation Res. 30 (1975) 63-70.
10. 2): I. Non-reciprocal genetic events occurring in leaf cells, Mutation Res. 25 (1974) 289-304.
11. J. Bříza, T. Gichner, J. Velemínský, Somatic mutations in tobacco plants after chronic exposure to maleic hydrazide and diethanolamine and potassium salts, Mutation Res. 139 (1984) 25-28.
12. M.J. Plewa, P.A. Dowd, E.D. Wagner, Calibration of the maize yg2 assay using gamma radiation and ethylmethane-sulfonate, Environ. Mutagen. 6 (1984) 781-795.
13. E.D. Wagner, A.L. Rayburn, D. Anderson, M.J. Plewa, Calibration of the single cell gel electrophoresis assay, flow cytometry analysis and forward mutation in Chinese hamster ovary cells, Mutagenesis 13 (1998) 81-84.
14. J.B. Majeska, H.E. Holden, Genotoxic effects of p-aminophenol in Chinese hamster ovary and mouse lymphoma cells: results of a multiple endpoint test, Environ. Mol. Mutagen. 26 (1995) 163-170.
15. A. Hartmann, G. Speit, Comparative investigations of the genotoxic effects of metals in the single cell gel (SCGE) assay and the sister chromatid exchange (SCE) test, Environ. Mol. Mutagen. 23 (1994) 299-305.
16. A. Hartmann, G. Speit, Genotoxic effects of chemicals in the single cell gel (SCG) test with human blood cells in relation to the induction of sister-chromatid exchanges (SCE), Mutation Res. 346 (1995) 49-56.
17. D.K. Monteith, J. Vanstone, Comparison of the microgel electrophoresis assay and other assays for genotoxicity in the detection of DNA damage, Mutation Res. 345 (1995) 97-103.
18. D. Slameňová, A. Gábelová, L. Ružeková, I. Chalupa, E. Horváthová, T. Farkašová, E. Bozsakyová, R. Štětina, Detection of MNNG-induced DNA lesions in mammalian cells: validation of comet assay against DNA unwinding technique, alkaline elution of DNA and chromosomal aberrations, Mutation Res. 383 (1997) 243-252.
19. D. Anderson, T.-W. Yu, M.A. Browne, The use of the same image analysis system to detect genetic damage in human lymphocytes treated with doxorubicin in the Comet and fluorescence in situ hybridisation (FISH) assays, Mutation Res. 390 (1997) 69-77.