DNA damage induced by mutagens in plant and human cell nuclei in acellular comet assay

Folia Histochemica et Cytobiologica

Volumn 44, Issue 2, 2006, Pages 127-131

  Juchimiuk, Jolanta ^a   Gnys, Agnieszka ^a   Maluszynska, Jolanta ^a  

^a UNIVERSITY OF SILESIA   (Poland)

Author keywords

Acellular comet assay; DNA damage; Leukocytes; Nicotiana tabacum

Indexed keywords

DNA; MALEIC HYDRAZIDE; METHYLNITROSOUREA; MUTAGENIC AGENT;

ARTICLE; CELL COMPOSITION; CELL ISOLATION; CELL NUCLEUS; COMET ASSAY; CONCENTRATION RESPONSE; CONTROLLED STUDY; DNA DAMAGE; GENOTOXICITY; HEALTH HAZARD; HUMAN; HUMAN CELL; LEUKOCYTE; MUTAGENESIS; NONHUMAN; PLANT CELL; RISK ASSESSMENT; SENSITIVITY ANALYSIS; TOBACCO;

EMBRYOPHYTA; NICOTIANA TABACUM;

EID: 33646408122     PISSN: 02398508     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (27)

1. Anderson D, Yu TW, Phillips BJ, Schmezer P (1994) The effects of various antioxidants and other modifying agents on oxygen-radical-generated DNA damage in human lymphocytes in the comet assay. Mutat Res 307: 261-271
2. Bender J (2004) DNA methylation and epigenetics. Annu Rev Plant Biol 55: 41-68
3. Cebulska-Wasilewska A (2000) Comparison of a plant assay and human lymphocytes as biomarkers of environmental pollution. In: Human Monitoring After Environmental and Occupational Exposure to Chemical and Physical Agents. Anderson D et al. [Eds], IOS Press 313, pp 268-277
4. Chuang Ch-H, Hu M-L (2004) Use of whole blood directly for single-cell gel electrophoresis (comet) assay in vivo and white blood cell for in vitro assay. Mutat Res 564: 75-82
5. Collins A, Dusinska M, Franklin N (1997) Comet assay in human biomonitoring studies: reliability, validation, and application. Env Mol Mutag 30: 139-146
6. Collins AR (2004) The comet assay for DNA damage and repair. Principles, application, and limitations. Mol Biotech 26: 249-261
7. Dolezel J, Greilhuber J, Lucretti S, Meister A, Lysak MA, Nardi L, Obermayer R (1998) Plant genome size estimation by flow cytometry: inter-laboratory comparison. Ann Bot 82: 17-26
8. Dulieu HL, Dalebroux MA (1975) Spontaneous and induced reversion rates in a double heterozygous mutant of Nicotiana tabacum var. xanthi N.C.-dose-response relationship. Mutat Res 30: 63-70
9. Gichner T, Plewa MJ (1998) Induction of somatic DNA damage as measured by single cell gel electrophoresis and point mutation in leaves of tobacco plants. Mutat Res 401: 143-152
10. Gichner T, Ptacek O, Stavreva DA, Plewa MJ (1999) Comparison of DNA damage in plants as measured by single cell gel electrophoresis and somatic leaf mutations induced by monofunctional alkalyting agents. Environ Mol Mutag 33: 279-286
11. Gichner T, Menke M, Stavreva DA, Schubert I (2000) Maleic hydrazide induces genotoxic effects but no damage detectable by the comet assay in tobacco and field beans. Mutagenesis 15: 385-389
12. Gichner T, Ptacek O, Stavreva DA, Wagner ED, Plewa MJ (2000) A comparison of DNA repair using the comet assay in tobacco seedlings after exposure to alkalyting agents or ionizing radiation. Mutat Res 470: 1-9
13. Gichner T, Muhlfeldova Z (2002) Induced DNA damage measured by the comet assay in 10 weed species. Biol Plant 45: 509-516
14. Gichner T (2003) DNA damage induced by indirect and direct acting mutagens in catalase-deficient transgenic tobacco cellular and acellular comet assay. Mutat Res 535: 187-193
15. Gichner T (2003) Diferential genotoxicity of ethyl methanesulphonate, N-ethyl-N nitrosourea and maleic hydrazide in tobacco seedlings based on data of the comet assay and two recombination assays. Mutat Res 538: 171-179
16. Jovtchev G, Menke M, Schubert I (2001) The comet assay detects adaptation to MNU-induced DNA damage in barley. Mutat Res 493: 95-100
17. Juchimiuk J, Maluszynska J (2005) Transformed roots of Crepis capillaris - a sensitive system for the evaluation of the clastogenicity of abiotic agents. Mutat Res 565: 129-138
18. Kasamatsu T, Kohda K, Kawazoe Y (1996) Comparison of chemically induced DNA breakage in cellular and subcellular systems using comet assay. Mutat Res 369: 1-6
19. Kassie F, Parzefall W, Knasmuller S (2000) Single cell gel electrophoresis assay: a new technique for human biomonitoring studies. Mutat Res 463: 13-31
20. Koppen G, Verschaeve L (1996) The alkaline comet test on plant cells: a new genotoxicity test for DNA strand breaks in Vicia faba cells. Mutat Res 360: 193-200
21. McKelvey-Martin VJ, Green MHL, Schmezer P, Pool-Zobel BL, De Meo MP, Collins A (1993) The single cell gel electrophoresis assay (comet assay): an European review. Mutat Res 288: 47-63
22. Ostling O, Johanson KJ (1984) Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells. Biochem Biophys Res Commun 123: 291-298
23. Poli P, Buschini A, Restivo FA, Ficarelli A, Cassoni IF, Ferrero I, Rossi C (1999) Comet assay application in environmental monitoring: DNA damage in human leukocytes and plant cells in comparison with bacterial and yeast tests. Mutagenesis 14: 547-555
24. Ribas G, Frenzili G, Barale R, Marcos R (1995) Herbicide-induced DNA damage in human lymphocytes evaluated by the single-cell gel electrophoresis (SCGE) assay. Mutat Res 344: 41-54
25. Santos AP, Abranches R, Stoger E, Beven A, Viegas W, Shaw PJ (2002) The architecture of interphase chromosomes and gene positioning are altered by changes in DNA methylation and histone acetylation. J Cell Sci 115: 4597-5605
26. Stavreva DA, Ptacek O, Plewa MJ, Gichner T (1998) Single cell gel electrophoresis analysis of genomic damage induced by ethyl methanesulfate in cultured tobacco cells. Mutat Res 422: 323-330
27. Tiersch TR, Chandler R, Wachtel SSM, Ellis S (1989) Reference standards for flow cytometry and application in comparative studies of nuclear DNA content. Cytometry 10: 706-710