Assessment of an extraction protocol to detect the major mastitis-causing pathogens in bovine milk

Journal of Dairy Science

Volumn 94, Issue 5, 2011, Pages 2171-2184

  Cressier, B ^a   Bissonnette, N ^a,b  

^a UNIVERSITY OF SHERBROOKE   (Canada)

^b SHERBROOKE RESEARCH AND DEVELOPMENT CENTRE   (Canada)

Author keywords

Bovine intramammary infection; Multiplex polymerase chain reaction (PCR); Sensitivity; Specificity

Indexed keywords

BACTERIA (MICROORGANISMS); BOS; BOVINAE; ESCHERICHIA COLI; KLEBSIELLA; KLEBSIELLA OXYTOCA; KLEBSIELLA PNEUMONIAE; STAPHYLOCOCCUS AUREUS; STREPTOCOCCUS AGALACTIAE; STREPTOCOCCUS DYSGALACTIAE; STREPTOCOCCUS UBERIS;

EID: 79955116088     PISSN: 00220302     EISSN: 15253198     Source Type: Journal    
DOI: 10.3168/jds.2010-3669     Document Type: Article

References (46)

1. Altschul S.F., Gish W., Miller W., Myers E.W., Lipman D.J. Basic local alignment search tool. J. Mol. Biol. 1990, 215:403-410.
2. Anglès d'Auriac M., Refseth U.H., Espelund M., Moi H., Størvold G., Jeansson S. A new automated method for isolation of Chlamydia trachomatis from urine eliminates inhibition and increases robustness for NAAT systems. J. Microbiol. Methods 2007, 70:416-423.
3. Bar D., Tauer L.W., Bennett G., González R.N., Hertl J.A., Schukken Y.H., Schulte H.F., Welcome F.L., Gröhn Y.T. The cost of generic clinical mastitis in dairy cows as estimated by using dynamic programming. J. Dairy Sci. 2008, 91:2205-2214.
4. Benson D.A., Karsch-Mizrachi I., Lipman D.J., Ostell J., Sayers E.W. GenBank. Nucleic Acids Res. 2010, 38(Database issue):D46-D51.
5. Biddle M.K., Fox L.K., Hancock D.D. Patterns of mycoplasma shedding in the milk of dairy cows with intramammary mycoplasma infection. J. Am. Vet. Med. Assoc. 2003, 223:1163-1166.
6. Brehm-Stecher B., Young C., Jaykus L.A., Tortorello M.L. Sample preparation: The forgotten beginning. J. Food Prot. 2009, 72:1774-1789.
7. Champagne C.P., Gardner N.J., Fontaine J., Richard J. Determination of viable bacterial populations in raw milk within 20 minutes by using a direct epifluorescent filter technique. J. Food Prot. 1997, 60:874-876.
8. Cole J.R., Wang Q., Cardenas E., Fish J., Chai B., Farris R.J., Kulam-Syed-Mohideen A.S., McGarrell D.M., Marsh T., Garrity G.M., Tiedje J.M. The Ribosomal Database Project: improved alignments and new tools for rRNA analysis. Nucleic Acids Res. 2009, 37(Database issue):D141-D145.
9. Cremonesi P., Castiglioni B., Malferrari G., Biunno I., Vimercati C., Moroni P., Morandi S., Luzzana M. Technical note: Improved method for rapid DNA extraction of mastitis pathogens directly from milk. J. Dairy Sci. 2006, 89:163-169.
10. Cremonesi P., Pisoni G., Severgnini M., Consolandi C., Moroni P., Raschetti M., Castiglioni B. Pathogen detection in milk samples by ligation detection reaction-mediated universal array method. J. Dairy Sci. 2009, 92:3027-3039.
11. Day S.P., Hudson A., Mast A., Sander T., Curtis M., Olson S., Chehak L., Quigley N., Ledford J., Yen-Lieberman B., Kohn D., Quigley D.I., Olson M. Analytical performance of the Investigational Use Only Cervista HPV HR test as determined by a multi-center study. J. Clin. Virol. 2009, 45(Suppl. 1):S63-S72.
12. Dingwell R.T., Leslie K.E., Schukken Y.H., Sargeant J.M., Timms L.L. Evaluation of the California mastitis test to detect an intramammary infection with a major pathogen in early lactation dairy cows. Can. Vet. J. 2003, 44:413-415.
13. Giese S.B., Ahrens P. Detection of Mycobacterium avium ssp. paratuberculosis in milk from clinically affected cows by PCR and culture. Vet. Microbiol. 2000, 77:291-297.
14. Gillespie B.E., Oliver S.P. Simultaneous detection of mastitis pathogens, Staphylococcus aureus, Streptococcus uberis, and Streptococcus agalactiae by multiplex real-time polymerase chain reaction. J. Dairy Sci. 2005, 88:3510-3518.
15. Giraffa G., Rossetti L., Neviani E. An evaluation of Chelex-based DNA purification protocols for the typing of lactic acid bacteria. J. Microbiol. Methods 2000, 42:175-184.
16. Graber H.U., Casey M.G., Naskova J., Steiner A., Schaeren W. Development of a highly sensitive and specific assay to detect Staphylococcus aureus in bovine mastitic milk. J. Dairy Sci. 2007, 90:4661-4669.
17. Grattepanche F., Lacroix C., Audet P., Lapointe G. Quantification by real-time PCR of Lactococcus lactis ssp. cremoris in milk fermented by a mixed culture. Appl. Microbiol. Biotechnol. 2005, 66:414-421.
18. Hotzel H., Sachse K., Pfützner H. Rapid detection of Mycoplasma bovis in milk samples and nasal swabs using the polymerase chain reaction. J. Appl. Bacteriol. 1996, 80:505-510.
19. International Dairy Federation. 1999. Suggested interpretation of mastitis terminology. Bull. No. 338:3-26. International Dairy Federation, Brussels, Belgium.
20. Kim C.H., Khan M., Morin D.E., Hurley W.L., Tripathy D.N., Kehrli M., Oluoch A.O., Kakoma I. Optimization of the PCR for detection of Staphylococcus aureus nuc gene in bovine milk. J. Dairy Sci. 2001, 84:74-83.
21. Koskinen M.T., Holopainen J., Pyörälä S., Bredbacka P., Pitkälä A., Barkema H.W., Bexiga R., Roberson J., Sølverød L., Piccinini R., Kelton D., Lehmusto H., Niskala S., Salmikivi L. Analytical specificity and sensitivity of a real-time polymerase chain reaction assay for identification of bovine mastitis pathogens. J. Dairy Sci. 2009, 92:952-959.
22. Kuang Y., Tani K., Synnott A.J., Ohshima K., Higuchi H., Nagahata H., Tanji Y. Characterization of bacterial population of raw milk from bovine mastitis by culture-independent PCR-DGGE method. Biochem. Eng. J. 2009, 45:76-81.
23. Longo M.C., Berninger M.S., Hartley J.L. Use of uracil DNA glycosylase to control carry-over contamination in polymerase chain reactions. Gene 1990, 93:125-128.
24. Luther D.A., Almeida R.A., Oliver S.P. Elucidation of the DNA sequence of Streptococcus uberis adhesion molecule gene (sua) and detection of sua in strains of Streptococcus uberis isolated from geographically diverse locations. Vet. Microbiol. 2008, 128:304-312.
25. Martin S.W. Estimating disease prevalence and the interpretation of screening. Prev. Vet. Med. 1984, 2:463-472.
26. Mayrl E., Roeder B., Mester P., Wagner M., Rossmanith P. Broad range evaluation of the matrix solubilization (matrix lysis) strategy for direct enumeration of foodborne pathogens by nucleic acids technologies. J. Food Prot. 2009, 72:1225-1233.
27. Minarovičová J., Lopašovská J., Valík L., Kuchta T. A method for the detection of Cryptosporidium parvum oocysts in milk based on microfiltration and real-time polymerase chain reaction. Food Anal. Methods 2010, 4:116-120.
28. Muramatsu Y., Yanase T., Okabayashi T., Ueno H., Morita C. Detection of Coxiella burnetii in cow's milk by PCR-enzyme-linked immunosorbent assay combined with a novel sample preparation method. Appl. Environ. Microbiol. 1997, 63:2142-2146.
29. Microbiological Procedures for Use in the Diagnosis of Bovine Udder Infection and Determination of Milk Quality 2004, NMC, National Mastitis Council, Verona, WI. 4th ed.
30. O'Mahony J., Hill C. Rapid real-time PCR assay for detection and quantitation of Mycobacterium avium ssp. paratuberculosis DNA in artificially contaminated milk. Appl. Environ. Microbiol. 2004, 70:4561-4568.
31. Olde Riekerink R.G.M., Barkema H.W., Kelton D.F., Scholl D.T. Incidence rate of clinical mastitis on Canadian dairy farms. J. Dairy Sci. 2008, 91:1366-1377.
32. Pruvost M., Grange T., Geigl E.-M. Minimizing DNA contamination by using UNG-coupled quantitative real-time PCR on degraded DNA samples: Application to ancient DNA studies. Biotechniques 2005, 38:569-575.
33. Quaglia N.C., Normanno G., Dambrosio A., Celano G.V., Parisi A., Firinu A., Buonavoglia C. Multiplex-touchdown PCR assay for the detection and genotyping of Helicobacter pylori from artificially contaminated sheep milk. J. Food Prot. 2005, 68:2136-2139.
34. Riyaz-Ul-Hassan S., Syed S., Johri S., Verma V., Qazi G.N. Application of a multiplex PCR assay for the detection of Shigella, Escherichia coli and Shiga toxin-producing Esch. coli in milk. J. Dairy Res. 2009, 76:188-194.
35. Roy J.-P., Du Tremblay D., DesCôteaux L., Messier S., Scholl D., Bouchard É. Effect of precalving intramammary treatment with pirlimycin in nulliparous Holstein heifers. Can. J. Vet. Res. 2007, 71:283-291.
36. Rudi K., Larsen F., Jakobsen K.S. Detection of toxin-producing cyanobacteria by use of paramagnetic beads for cell concentration and DNA purification. Appl. Environ. Microbiol. 1998, 64:34-37.
37. Santos O.C., Barros E.M., Brito M.A., Bastos Mdo C., Dos Santos K.R., Giambiagi-Demarval M. Identification of coagulase-negative staphylococci from bovine mastitis using RFLP-PCR of the groEL gene. Vet. Microbiol. 2008, 130:134-140.
38. Schukken Y.H., Smit J.A.H., Grommers F.J., Vandegeer D., Brand A. Effect of freezing on bacteriologic culturing of mastitis milk samples. J. Dairy Sci. 1989, 72:1900-1906.
39. Siddiqui H., Nederbragt A.J., Jakobsen K.S. A solid-phase method for preparing human DNA from urine for diagnostic purposes. Clin. Biochem. 2009, 42:1128-1135.
40. Supré K., De Vliegher S., Sampimon O.C., Zadoks R.N., Vaneechoutte M., Baele M., De Graef E., Piepers S., Haesebrouck F. Technical note: Use of transfer RNA-intergenic spacer PCR combined with capillary electrophoresis to identify coagulase-negative Staphylococcus species originating from bovine milk and teat apices. J. Dairy Sci. 2009, 92:3204-3210.
41. Tan S.C., Yiap B.C. DNA, RNA, and protein extraction: The past and the present. J. Biomed. Biotechnol. 2009, 2009:574398.
42. Taponen S., Salmikivi L., Simojoki H., Koskinen M.T., Pyörälä S. Real-time polymerase chain reaction-based identification of bacteria in milk samples from bovine clinical mastitis with no growth in conventional culturing. J. Dairy Sci. 2009, 92:2610-2617.
43. Therese K.L., Anand A.R., Madhavan H.N. Polymerase chain reaction in the diagnosis of bacterial endophthalmitis. Br. J. Ophthalmol. 1998, 82:1078-1082.
44. Weisburg W.G., Barns S.M., Pelletier D.A., Lane D.J. 16S ribosomal DNA amplification for phylogenetic study. J. Bacteriol. 1991, 173:697-703.
45. Wilson K. Preparation of genomic DNA from bacteria. Current Protocols Molecular Biology 2001, 1-5. John Wiley and Sons Inc., Mississauga, ON, Canada, Chapter 2: Unit 2 4. F.M. Ausubel, R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith, K. Struhl (Eds.).
46. Zadoks R.N., Watts J.L. Species identification of coagulase-negative staphylococci: Genotyping is superior to phenotyping. Vet. Microbiol. 2009, 134:20-28.