Application of a multiplex PCR assay for the detection of shigella, escherichia coli and shiga toxin-producing esch. coli in milk

Journal of Dairy Research

Volumn 76, Issue 2, 2009, Pages 188-194

  Riyaz Ul Hassan, Syed ^a   Syed, Saima ^a   Johri, Sarojini ^a   Verma, Vijeshwar ^a   Qazi, Ghulam Nabi ^a  

^a CSIR INDIAN INSTITUTE OF INTEGRATIVE MEDICINE   (India)

Author keywords

Esch. coli; Milk; Multiplex PCR; Shigella; STEC

Indexed keywords

SHIGA TOXIN;

ANIMAL; ARTICLE; CATTLE; CLASSIFICATION; ESCHERICHIA COLI; GENETIC MARKER; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MICROBIOLOGY; MILK; POLYMERASE CHAIN REACTION; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; SHIGELLA;

ANIMALS; CATTLE; ESCHERICHIA COLI; GENETIC MARKERS; MILK; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SHIGA TOXINS; SHIGELLA;

ESCHERICHIA COLI; SHIGELLA;

EID: 68349139671     PISSN: 00220299     EISSN: 14697629     Source Type: Journal    
DOI: 10.1017/S0022029909004026     Document Type: Article

References (37)

1. Aranda KRS, Fagundes-Neto U & Scaletsky ICA 2004 Evaluation of Multiplex PCRs for diagnosis of infection with diarrheagenic Escherichia coli and Shigella spp. Journal of Clinical Microbiology 42 5849-5853
2. Aslam M, Hogan J & Smith KL 2003 Development of a PCR-based assay to detect shiga toxin-producing Escherichia coli, Listeria monocytogenes, and Salmonella in milk. Food Microbiology 20 345-350
3. Bej AK, DiCesare JL, Haff L & Atlas RM 1991 Detection of E. coli and Shigella spp. in water by using the polymerase chain reaction and gene probes for uid. Applied and Environmental Microbiology 57 1013-1017
4. Bettelheim K 1997 Escherichia coli O157 outbreak in Japan: lessons for Australia. Australian Veterinary Journal 75 108
5. Blanco M, Blanco JE, Mora A, Rey J, Alonso JM, Herrmoso M, Hermoso J, Alonso MP et al. 2003 Serotypes, virulence genes, and intimin types of Shiga toxin (verotoxin)-producing Escherichia coli isolated from healthy sheep from Spain. Journal of Clinical Microbiology 41 1351-1356
6. Boerlin P, McEwen SA, Boerlin-Petzold F, Wilson JB, Johnson RP & Gyles CI 1999 Association between virulence factors of Shiga toxin- producing Escherichia coli and disease in humans. Journal ofClinical Microbiology 37 497-503
7. Bradbury J 1997 Report on Scottish E. coli O157 outbreak released. Lancet 349 1073
8. Brett KN, Ramachandran V, Hornitzky MA, Bettelheim KA, Walker MJ & Djordjevic SP 2003 Stx1c is the most common shiga toxin 1 subtype among shiga toxin producing Escherichia coli isolates from sheep but not isolates from cattle. Journal of Clinical Microbiology 41 926-936
9. Buysse JM, Stover CK, Oaks EV, Venkatesan M & Kopecko DJ 1987 Molecular cloning of invasion plasmid antigen (ipa) genes from Shigella flexneri: analysis of ipa gene products and genetic mapping. Journal of Bacteriology 169 2561-2569
10. Centers for Disease Control 1993 Update: multistate outbreak of Escherichia coli O157:H7 infections from hamburgers western United States, 1992-1 993. Morbid and Mortal Weekly Rep 42 258-263
11. Centers for Disease Control 2005 Bacterial Foodborne and Diarrheal Disease National Case Surveillance. Annual Report. Atlanta: U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, 2007
12. Centers for Disease Control 2007 Escherichia coli O157:H7 infection associated with drinking raw milk-Washington and Oregan, Nov.- Dec. 2005. Morbidity and Mortality Weekly Report Mar. 2. 56 165-167
13. Cristancho L, Johnson RP, McEwen SA & Gyles CL 2008 Escherichia coli O157: H7 and other shiga toxin-producing E. coli in white veal calves. Veterinary Microbiology 126 200-209
14. Ethelberg S, Olsen KE, Scheutz F, Jensen C, Schiellerup P, Enberg J, Petersen AM, Olesen B, Gerner-Smidt P & Molbak K 2004 Virulence factors for hemolytic uremic syndrome, Denmark. EmergingInfectious Diseases 10 842-847
15. Gerber A, Karch H, Allerberger F, Verweyen HM & Zimmerhackl LB 2002 Clinical course and the role of shiga toxin-producing Escherichia coli infection in the hemolytic-uremic syndrome in pediatric patients, 1997-2000, in Germany and Austria: a prospective study. Journal of Infectious Diseases 186 493-500
16. Gracia-Fulgueiras A, Sanchez S, Guillen JJ, Marsilla B, Aladuena A & Novarro C 2001 A large outbreak of Shigella sonnei gastroenterititis associated with consumption of fresh pasteurized milk cheese. European Journal of Epidemiology 17 533-538
17. Griffin PM & Tauxe RV 1991 The epidemiology of infections caused by Escherichia coli O157:H7, other enterohemorrhagic E. coli, and the associated hemolytic uremic syndrome. Epidemiologic Reviews 13 60-98
18. Hartman AB, Venkatesan M, Oaks EV & Buysee JM 1990 Sequence and molecular characterization of multicopy invasion plasmid antigen gene, ipaH,ofShigella flexneri. Journal of Bacteriology 172 1905-1915
19. Heijnen L & Medema G 2006 Quantitative detection of E. coli, E. coli O157:H7 and other shiga toxin producing E. coli in water samples using culture method combined with real-time PCR. Journal of Water Health 4 487-498
20. Jochimsen B, Nygaard P & Vestergaard T 1975 Location on the chromosome of Escherichia coli of genes governing purine metabolism. Molecular and General Genetics 143 85-91
21. Karmali MA 1989 Infections by verocytotoxin-producing Escherichia coli. Clinical Microbiology Reviews 2 15-38
22. Koneman EW, Allen SD, Janda WM, Schreckenberger PC & Winn WC 1997 The enterobacteraiceae. In Color Atlas and Diagnostic Microbiology, V Edn. pp. 171-241 (Ed. A. Allen). Lippincott Williams and Wilkins, Philadelphia, USA
23. Lampel KA, Orlandi PA & Kornegay L 2000 Improved template preparation for PCR-based assay for detection of food-borne bacterial pathogens. Applied and Environmental Microbiology 66 4539-4542
24. Novel G & Novel M 1973 Mutants d' Escherichia coli K-12 affectes pour leur croissance sur methyl b-D-glucuronide: localisation du gene de structure de la P-Dglucuronidase (uidA). Molecular and General Genetics 120 319-335
25. Olsen JE, Aabo S, Hill W, Notermans S, Wernars K, Granum PE, Popovic T, Rasmussen HN & Olsvik O 1995 Probes and polymerase chain reaction for detection of food-borne bacterial pathogens. International Journal Food Microbiology 28 1-78
26. Paton JC & Paton AW 1998 Pathogenesis and diagnosis of Shiga toxin- producing Escherichia coli infections. Clinical Microbiology Reviews 11 450-479
27. Perelle S, Dilasser F, Grout J & Fach P 2007 Screening food raw material for the presence of the world's frequent clinical cases of shiga toxin- encoding Escherichia coli O26, O103, O111, O145 and O157. International Journal of Food Microbiology 113 284-288
28. Rozen S & Skaletsky HJ 2000 Primer3 on the WWW for general users and for biologist programmers. In Bioinformatics Methods and Protocols: Methods in Molecular Biology, pp. 365-386 (Eds S Krawetz & 5 Misener). Totowa, NJ: Humana Press
29. Saiki RK 1989 The design and optimization of the polymerase chain reaction. In PCR Technology: Principles and Applications for DNA Amplification, pp. 7-22 (Ed. HA Ehrlich), New York, Stockton
30. Stefan A, Scaramagli S, Bergami R, Mazzini C, Barnanera M, Perelle S 6 Fach P 2007 Real-time PCR and enzyme-linked fluorescent assay methods for detecting Shiga-toxin-producing Escherichia coli in mincemeat samples. Canadian Journal of Microbiology 53 337-342
31. Versalovic J & Lupski R 2002 Molecular detection and genotyping of pathogens: more accurate and rapid answers. Trends in Microbiology 10 S15-S21
32. Villalobo E & Torres A 1998 PCR for the detection of Shigella spp. in Mayonnaise. Applied and Environmental Microbiology 64 1242-1245
33. Wang L, Li Y & Mustaphai A 2007 Rapid and simultaneous quantification of Escherichia coli O157:H7, Salmonella, andShigella in ground beef by multiplex real-time PCR and immunomagnetic separation. Journal Food Protection 70 1366-1372
34. Wani SA, Pandit F, Samanta I, Bhat MA & Buchh AS 2004 Molecular epidemiology of Shiga toxin -producing Escherichia coli in India. Current Science 87 1345-1353
35. Werber D, Fruth A, Buchholz U, Prager R, Kramer MH, Ammon A & Tschape H 2003 Strong association between shiga toxin-producing Escherichia coli O157 and virulence genes stx2 and eae as possible explanation for predominance of serogroup O157 in patients with haemolytic uraemic syndrome. European Journal of Clinical Microbiology and Infectious Diseases 22 726-730
36. Yang YG, Song MK, Park SJ & Kim SW 2007 Direct detection of Shigella flexneri and Salmonella typhimurium in human feces by real-time PCR. Journal of Microbiology and Biotechnology 17 1616-1621
37. Zagrebneviene G, Jasulaitiene V, Morkunas B, Tarbunas S & Ladygaite J 2005 Shigella sonnei outbreak due to consumption of unpasteurized milk curds in Vilnius, Lithuania, 2004. Euro Surveillance 2005;10(12): E051201.3. Available from: http://www.eurosurveillance.org/ew/2005/ 051201.asp#3