A new automated method for isolation of Chlamydia trachomatis from urine eliminates inhibition and increases robustness for NAAT systems

Journal of Microbiological Methods

Volumn 70, Issue 3, 2007, Pages 416-423

  d'Auriac, Marc Anglès ^a   Refseth, Unn Hilde ^a   Espelund, Mari ^a   Moi, Harald ^b   Størvold, Gunnar ^c   Jeansson, Stig ^c  

^a Genpoint AS   (Norway)

^b UNIVERSITY OF OSLO   (Norway)

^c ULLEVÅL UNIVERSITY HOSPITAL   (Norway)

Author keywords

Automation; Chlamydia trachomatis; NAAT; SDA; STI; Urine

Indexed keywords

BACTERIAL DNA; NUCLEIC ACID;

ADOLESCENT; ADULT; ANALYTIC METHOD; ARTICLE; AUTOMATION; BACTERIUM ISOLATION; CHLAMYDIA TRACHOMATIS; CONTROLLED STUDY; DNA DETERMINATION; HUMAN; MAGNETISM; NUCLEIC ACID AMPLIFICATION; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; URINALYSIS;

ADOLESCENT; ADULT; CHLAMYDIA INFECTIONS; CHLAMYDIA TRACHOMATIS; DNA, BACTERIAL; FEMALE; HUMANS; MALE; MIDDLE AGED; NUCLEIC ACID AMPLIFICATION TECHNIQUES; SENSITIVITY AND SPECIFICITY;

BACTERIA (MICROORGANISMS); CHLAMYDIA TRACHOMATIS;

EID: 34548124655     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2007.05.017     Document Type: Article

References (26)

1. Aldeen T., Haghdoost A., and Hay P. Urine based screening for asymptomatic/undiagnosed genital chlamydial infection in young people visiting the accident and emergency department is feasible, acceptable, and can be epidemiologically helpful. Sex. Transm. Infect. 79 (2003) 229-233
2. Chan E.L., Brandt K., Olienus K., Antonishyn N., and Horsman G.B. Performance characteristics of the Becton Dickinson ProbeTec System for direct detection of Chlamydia trachomatis and Neisseria gonorrhoeae in male and female urine specimens in comparison with the Roche Cobas System. Arch. Pathol. Lab. Med. 124 (2000) 1649-1652
3. Chernesky M., Jang D., Chong S., Sellors J., and Mahony J. Impact of urine collection order on the ability of assays to identify Chlamydia trachomatis infections in men. Sex. Transm. Dis. 30 (2003) 345-347
4. Cosentino L.A., Landers D.V., and Hillier S.L. Detection of Chlamydia trachomatis and Neisseria gonorrhoeae by strand displacement amplification and relevance of the amplification control for use with vaginal swab specimens. J. Clin. Microbiol. 41 (2003) 3592-3596
5. Cotter D.A. The safe use of dimethyl sulfoxide in the laboratory. Am. J. Med. Technol. 41 (1975) 197-198
6. Culler E.E., Caliendo A.M., and Nolte F.S. Reproducibility of positive test results in the BDProbeTec ET system for detection of Chlamydia trachomatis and Neisseria gonorrhoeae. J. Clin. Microbiol. 41 (2003) 3911-3914
7. Gaydos C.A., Theodore M., Dalesio N., Wood B.J., and Quinn T.C. Comparison of three nucleic acid amplification tests for detection of Chlamydia trachomatis in urine specimens. J. Clin. Microbiol. 42 (2004) 3041-3045
8. Gronowski A.M., Copper S., Baorto D., and Murray P.R. Reproducibility problems with the Abbott laboratories LCx assay for Chlamydia trachomatis and Neisseria gonorrhoeae. J. Clin. Microbiol. 38 (2000) 2416-2418
9. Groseclose S.L., Brathwaite W.S., Hall P.A., Connor F.J., Sharp P., Anderson W.J., Fagan R.F., Aponte J.J., Jones G.F., Nitschke D.A., Chang M.H., Doyle T., Dhara R., Jajosky R.A., and Hatmaker J.D. Summary of notifiable diseases - United States, 2002. Morb. Mortal. Wkly. Rep. 51 (2004) 1-84
10. LaMontagne D.S., Fenton K.A., Randall S., Anderson S., and Carter P. Establishing the National Chlamydia Screening Programme in England: results from the first full year of screening. Sex. Transm. Infect. 80 (2004) 335-341
11. LaMontagne D.S., Fine D.N., and Marrazzo J.M. Chlamydia trachomatis infection in asymptomatic men. Am. J. Prev. Med. 24 (2003) 36-42
12. Little M.C., Andrews J., Moore R., Bustos S., Jones L., Embres C., Durmowicz G., Harris J., Berger D., Yanson K., Rostkowski C., Yursis D., Price J., Fort T., Walters A., Collis M., Llorin O., Wood J., Failing F., O'Keefe C., Scrivens B., Pope B., Hansen T., Marino K., and Williams K. Strand displacement amplification and homogeneous real-time detection incorporated in a second-generation DNA probe system, BDProbeTec ET. Clin. Chem. 45 (1999) 777-784
13. Low N., McCarthy A., Macleod J., Salisbury C., Horner P.J., Roberts T.E., Campbell R., Herring A., Skidmore S., Sanford E., Sterne J.A., Davey S.G., Graham A., Huengsberg M., Ross J., and Egger M. The chlamydia screening studies: rationale and design. Sex. Transm. Infect. 80 (2004) 342-348
14. Mahony J., Chong S., Jang D., Luinstra K., Faught M., Dalby D., Sellors J., and Chernesky M. Urine specimens from pregnant and nonpregnant women inhibitory to amplification of Chlamydia trachomatis nucleic acid by PCR, ligase chain reaction, and transcription-mediated amplification: identification of urinary substances associated with inhibition and removal of inhibitory activity. J. Clin. Microbiol. 36 (1998) 3122-3126
15. Marrazzo J.M., White C.L., Krekeler B., Celum C.L., Lafferty W.E., Stamm W.E., and Handsfield H.H. Community-based urine screening for Chlamydia trachomatis with a ligase chain reaction assay. Ann. Intern. Med. 127 (1997) 796-803
16. Morre S.A., Sillekens P.T., Jacobs M.V., de Blok S., Ossewaarde J.M., van Aarle P., van Gemen B., Walboomers J.M., Meijer C.J., and van den Brule A.J. Monitoring of Chlamydia trachomatis infections after antibiotic treatment using RNA detection by nucleic acid sequence based amplification. Mol. Pathol. 51 (1998) 149-154
17. Mulcahy G.M., Albanese E.A., and Bachl B.L. Reproducibility of the Roche Amplicor polymerase chain reaction assay for detection of infection by Chlamydia trachomatis in endocervical specimens. Clin. Chem. 44 (1998) 1575-1578
18. Paavonen J., and Lehtinen M. Chlamydial pelvic inflammatory disease. Hum. Reprod. Updat. 2 (1996) 519-529
19. Pack R.P., Diclemente R.J., Hook III E.W., and Oh M.K. High prevalence of asymptomatic STDs in incarcerated minority male youth: a case for screening. Sex. Transm. Dis. 27 (2000) 175-177
20. Rudi K., Kroken M., Dahlberg O.J., Deggerdal A., Jakobsen K.S., and Larsen F. Rapid, universal method to isolate PCR-ready DNA using magnetic beads. Biotechniques 22 (1997) 506-511
21. Schachter J., Chow J.M., Howard H., Bolan G., and Moncada J. Detection of Chlamydia trachomatis by nucleic acid amplification testing: our evaluation suggests that CDC-recommended approaches for confirmatory testing are ill-advised. J. Clin. Microbiol. 44 (2006) 2512-2517
22. Sutton T.L., Martinko T., Hale S., and Fairchok M.P. Prevalence and high rate of asymptomatic infection of Chlamydia trachomatis in male college reserve officer training corps cadets. Sex. Transm. Dis. 30 (2003) 901-904
23. Templeton K., Roberts J., Jeffries D., Forster G., and Aitken C. The detection of Chlamydia trachomatis by DNA amplification methods in urine samples from men with urethritis. Int. J. STD AIDS 12 (2001) 793-796
24. Van Der P.B., Ferrero D.V., Buck-Barrington L., Hook III E., Lenderman C., Quinn T., Gaydos C.A., Lovchik J., Schachter J., Moncada J., Hall G., Tuohy M.J., and Jones R.B. Multicenter evaluation of the BDProbeTec ET System for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine specimens, female endocervical swabs, and male urethral swabs. J. Clin. Microbiol. 39 (2001) 1008-1016
25. Verkooyen R.P., Noordhoek G.T., Klapper P.E., Reid J., Schirm J., Cleator G.M., Ieven M., and Hoddevik G. Reliability of nucleic acid amplification methods for detection of Chlamydia trachomatis in urine: results of the first international collaborative quality control study among 96 laboratories. J. Clin. Microbiol. 41 (2003) 3013-3016
26. Watson E.J., Templeton A., Russell I., Paavonen J., Mardh P.A., Stary A., and Pederson B.S. The accuracy and efficacy of screening tests for Chlamydia trachomatis: a systematic review. J. Med. Microbiol. 51 (2002) 1021-1031