Construction of an Escherichia coli mutant producing monophosphoryl lipid A

Biotechnology Letters

Volumn 33, Issue 5, 2011, Pages 1013-1019

  Chen, Jiuzhou ^a   Tao, Guanjun ^a   Wang, Xiaoyuan ^a  

^a JIANGNAN UNIVERSITY   (China)

Author keywords

Adjuvant; Chromosomal integration; Endotoxin; Lipid A; Lipopolysaccharide; Monophosphoryl lipid A; Salmonella minnesota

Indexed keywords

ADJUVANT; CHROMOSOMAL INTEGRATION; ENDOTOXIN; LIPID A; LIPOPOLYSACCHARIDES; MONOPHOSPHORYL LIPID A;

CHROMATOGRAPHIC ANALYSIS; GENES; INTEGRATION; MASS SPECTROMETRY; SALMONELLA; THIN LAYER CHROMATOGRAPHY;

ESCHERICHIA COLI;

DRUG DERIVATIVE; LIPID A; PHOSPHATASE; PHOSPHORYL LIPID A;

ARTICLE; BACTERIUM TRANSFORMATION; BIOSYNTHESIS; CHEMISTRY; ELECTROSPRAY MASS SPECTROMETRY; ENZYMOLOGY; ESCHERICHIA COLI; GENETIC RECOMBINATION; GENETICS; METABOLISM; SALMONELLA; THIN LAYER CHROMATOGRAPHY;

CHROMATOGRAPHY, THIN LAYER; ESCHERICHIA COLI; LIPID A; PHOSPHORIC MONOESTER HYDROLASES; RECOMBINATION, GENETIC; SALMONELLA; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TRANSFORMATION, BACTERIAL;

ESCHERICHIA COLI; NEGIBACTERIA; SALMONELLA ENTERICA SUBSP. ENTERICA SEROVAR MINNESOTA;

EID: 79955111925     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-011-0521-z     Document Type: Article

References (25)

1. Albermann C, Trachtmann N, Sprenger GA (2010) A simple and reliable method to conduct and monitor expression cassette integration into the Escherichia coli chromosome. Biotechnol J 5: 32-38.
2. Baldridge JR, Crane RT (1999) Monophosphoryl lipid A (MPL) formulations for the next generation of vaccines. Methods 19: 103-107.
3. Bosshart H, Heinzelmann M (2007) Targeting bacterial endotoxin: two sides of a coin. Ann N Y Acad Sci 1096: 1-17.
4. Cherepanov PP, Wackernagel W (1995) Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant. Gene 158: 9-14.
5. Datsenko KA, Wanner BL (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci USA 97: 6640-6645.
6. De Mey M, Maertens J, Boogmans S, Soetaert WK, Vandamme EJ, Cunin R, Foulquie-Moreno MR (2010) Promoter knock-in: a novel rational method for the fine tuning of genes. BMC Biotechnol 10: 26.
7. Lee CH, Tsai CM (1999) Quantification of bacterial lipopolysaccharides by the purpald assay: measuring formaldehyde generated from 2-keto-3-deoxyoctonate and heptose at the inner core by periodate oxidation. Anal Biochem 267: 161-168.
8. Li P, Sun M, Wohland T, Ho B, Ding JL (2006) The molecular mechanism of interaction between sushi peptide and Pseudomonas endotoxin. Cell Mol Immunol 3: 21-28.
9. Mata-Haro V, Cekic C, Martin M, Chilton PM, Casella CR, Mitchell TC (2007) The vaccine adjuvant monophosphoryl lipid A as a TRIF-biased agonist of TLR4. Science 316: 1628-1632.
10. Munford RS (2006) Severe sepsis and septic shock: the role of gram-negative bacteremia. Annu Rev Pathol 1: 467-496.
11. Okemoto K, Kawasaki K, Hanada K, Miura M, Nishijima M (2006) A potent adjuvant monophosphoryl lipid A triggers various immune responses, but not secretion of IL-1beta or activation of caspase-1. J Immunol 176: 1203-1208.
12. Park BS, Song DH, Kim HM, Choi BS, Lee H, Lee JO (2009) The structural basis of lipopolysaccharide recognition by the TLR4-MD-2 complex. Nature 458: 1191-1195.
13. Persing DH, Coler RN, Lacy MJ, Johnson DA, Baldridge JR, Hershberg RM, Reed SG (2002) Taking toll: lipid A mimetics as adjuvants and immuno modulators. Trends Microbiol 10: S32-S37.
14. Raetz CRH, Whitfield C (2002) Lipopolysaccharide endotoxins. Annu Rev Biochem 71: 635-700.
15. Raetz CRH, Reynolds CM, Trent MS, Bishop RE (2007) Lipid A modification systems in gram-negative bacteria. Annu Rev Biochem 76: 295-329.
16. Reed SG, Bertholet S, Coler RN, Friede M (2009) New horizons in adjuvants for vaccine development. Trends Immunol 30: 23-32.
17. Sharan SK, Thomason LC, Kuznetsov SG, Court DL (2009) Recombineering: a homologous recombination-based method of genetic engineering. Nat Protoc 4: 206-223.
18. Takayama K, Ribi E, Cantrell JL (1981) Isolation of a nontoxic lipid A fraction containing tumor regression activity. Cancer Res 41: 2654-2657.
19. Tan NS, Ng ML, Yau YH, Chong PK, Ho B, Ding JL (2000) Definition of endotoxin binding sites in horseshoe crab Factor C recombinant sushi proteins and neutralization of endotoxin by sushi peptides. FASEB J 14: 1801-1813.
20. Wang X, Quinn PJ (2010) Lipopolysaccharide: biosynthetic pathway and structure modification. Prog Lipid Res 49: 97-107.
21. Wang X, Karbarz MJ, McGrath SC, Cotter RJ, Raetz CRH (2004) MsbA transporter-dependent lipid A 1-dephosphorylation on the periplasmic surface of the inner membrane: topography of Francisella novicida LpxE expressed in Escherichia coli. J Biol Chem 279: 49470-49478.
22. Wang X, Ribeiro AA, Guan Z, McGrath SC, Cotter RJ, Raetz CRH (2006) Structure and biosynthesis of free lipid A molecules that replace lipopolysaccharide in Francisella novicida. Biochemistry 45: 14427-14440.
23. Wang X, Ribeiro AA, Guan Z, Abraham SN, Raetz CRH (2007) Attenuated virulence of a Francisella mutant lacking the lipid A 4′-phosphatase. Proc Natl Acad Sci USA 104: 4136-4141.
24. Wang X, Ribeiro AA, Guan Z, Raetz CRH (2009) Identification of undecaprenyl phosphate-beta-d-galactosamine in Francisella novicida and its function in lipid A modification. Biochemistry 48: 1162-1172.
25. Westphal O, Jann K (1965) Bacterial lipopolysaccharides. Extraction with phenol water and further applications of the procedure. In: Whistler RL (ed) Methods in carbohydrate chemistry. Academic Press, New York, pp 83-91.