Multiplex polymerase chain reaction-based assay for the specific detection of toxin-producing Vibrio cholerae in fish and fishery products

Applied Microbiology and Biotechnology

Volumn 90, Issue 3, 2011, Pages 1111-1118

  Jeyasekaran, Geevaretnam ^a   Thirumalai Raj, Kannan ^a   Jeya Shakila, Robinson ^a   Jemila Thangarani, Albin ^a   Sukumar, Durairaj ^a  

^a MADRAS VETERINARY COLLEGE   (India)

Author keywords

Multiplex PCR; rpoA gene; Toxin genes ctxA and RtxA genes; Vibrio, toxigenic strains of V. cholerae

Indexed keywords

BACTERIAL PATHOGENS; CHOLERA TOXIN; ENVIRONMENTAL SAMPLE; FISHERY PRODUCT; MULTIPLEX PCR; PRE-ENRICHMENT; RNA POLYMERASE; RPOA GENE; SIMULTANEOUS DETECTION; SPECIFIC DETECTION; STEP DETECTION; TOXIGENIC; TOXIGENIC STRAINS; TOXIN GENES; VIBRIO CHOLERAE;

GENES; MULTIPLEXING; POLYMERIZATION; POLYMERS; RNA; TOXIC MATERIALS;

POLYMERASE CHAIN REACTION;

CHOLERA TOXIN;

BACTERIUM; BIOASSAY; CRUSTACEAN; DETECTION METHOD; DILUTION; FISH; FISHERY; GENETIC ANALYSIS; PATHOGEN; POLYMERASE CHAIN REACTION; SENSITIVITY ANALYSIS; TOXIN;

ARTICLE; BACTERIAL GENE; BACTERIUM DETECTION; CTXA GENE; FISH; GENE AMPLIFICATION; LIMIT OF DETECTION; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; RPOA GENE; RTXA GENE; SENSITIVITY AND SPECIFICITY; SHRIMP; VIBRIO CHOLERAE;

ANIMALS; BACTERIAL PROTEINS; CHOLERA TOXIN; FISH PRODUCTS; FISHES; FOOD CONTAMINATION; PENAEIDAE; POLYMERASE CHAIN REACTION; SEAFOOD; VIBRIO CHOLERAE;

BACTERIA (MICROORGANISMS); DECAPODA (CRUSTACEA); VIBRIO; VIBRIO CHOLERAE;

EID: 79954961327     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-011-3175-9     Document Type: Article

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