Direct detection of Vibrio cholerae and ctxA in Peruvian coastal water and plankton by PCR

Applied and Environmental Microbiology

Volumn 69, Issue 6, 2003, Pages 3676-3680

  Lipp, Erin K ^a,e   Rivera, Irma N G ^b   Gil, Ana I ^c   Espeland, Eric M ^a   Choopun, Nipa ^a   Louis, Valérie R ^a   Russek Cohen, Estelle ^d   Huq, Anwar ^a,d   Colwell, Rita R ^a,d  

^a UNIVERSITY OF MARYLAND BIOTECHNOLOGY INSTITUTE   (United States)

^b UNIVERSITY OF SÃO PAULO   (Brazil)

^c INSTITUTO DE INVESTIGACIÓN NUTRICIONAL   (Peru)

^d UNIVERSITY OF MARYLAND   (United States)

^e UNIVERSITY OF GEORGIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; DNA; RESERVOIRS (WATER); SEAWATER; TOXICITY;

COASTAL WATERS;

MICROBIOLOGY;

SEA WATER;

BACTERIUM; COASTAL WATER; DETECTION METHOD; PATHOGEN; POLYMERASE CHAIN REACTION;

ARTICLE; BACTERIAL GENE; BACTERIUM DETECTION; COASTAL WATERS; CTXA GENE; DNA EXTRACTION; GENE IDENTIFICATION; NONHUMAN; PERU; PLANKTON; POLYMERASE CHAIN REACTION; SEASONAL VARIATION; VIBRIO CHOLERAE; WATER ANALYSIS; WATER CONTAMINATION; WATER QUALITY; WATER SAMPLING;

(SOUTHEAST); PACIFIC OCEAN; PERU;

BACTERIA (MICROORGANISMS); NEGIBACTERIA; VIBRIO; VIBRIO CHOLERAE;

EID: 0037978076     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.69.6.3676-3680.2003     Document Type: Article

References (26)

1. Amann, R. I., W. Ludweg, and K. Schleifer. 1995. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol. Rev. 59:143-169.
2. Chun, J., A. Huq, and R. R. Colwell. 1999. Analysis of 16S-23S rRNA intergenic spacer regions of Vibrio cholerae and Vibrio mimicus. Appl. Environ. Microbiol. 65:2202-2208.
3. Colwell, R. R., J. Kaper, and S. W. Joseph. 1977. Vibrio cholerae and Vibrio parahaemolyticus and other vibrios: occurrence and distribution in Chesapeake Bay. Science 198:394-396.
4. Colwell, R. R., R. J. Seidler, J. Kaper, S. W. Joseph, S. Garges, H. Lockman, D. Maneval, H. Bradford, N. Roberts, E. Remmers, I. Huq, and A. Huq. 1981. Occurrence of Vibrio cholerae serotype O1 in Maryland and Louisiana estuaries. Appl. Environ. Microbiol. 41:555-558.
5. Faruque, S. M., M. N. Saha Asadulghani, D. A. Sack, R. B. Sack, Y. Takeda, and G. B. Nair. 2000. The O139 serogroup of Vibrio cholerae comprises diverse clones of epidemic and nonepidemic strains derived from multiple V. cholerae O1 or non-O1 progenitors. J. Infect. Dis. 182:1161-1168.
6. Fields, P. I., T. Popovic, K. Wachsmuth, and O. Olsvik. 1992. Use of polymerase chain reaction for detection of toxigenic Vibrio cholerae O1 strains from the Latin American cholera epidemic. J. Clin. Microbiol. 30:2118-2121.
7. Franco, A. A., A. D. Fix, A. Prada, E. Paredes, J. C. Palomino, A. C. Wright, J. A. Johnson, R. McCarter, H. Guerra, and J. G. Morris, Jr. 1997. Cholera in Lima, Peru, correlates with prior isolation of Vibrio cholerae from the environment. Am. J. Epidemiol. 146:1067-1075.
8. Heidelberg, J. F. 1997. Seasonal abundance of bacterioplankton populations of bacteria, gamma proteobacteria, Vibrio/Photobacterium, Vibrio vulnificus, Vibrio cholerae/vibrio mimicus, and Vibrio cincinnatiensis associated with zooplankton in the Choptank River, Maryland. Ph.D. thesis. University of Maryland, College Park.
9. Heidelberg, J. F., K. B. Heidelberg, and R. R. Colwell. 2002. Seasonality of Chesapeake Bay bacterioplankton species. Appl. Environ. Microbiol. 68: 5488-5497.
10. Hoshino, K., S. Yamasaki, A. K. Mukhopadhyay, S. Chakraborty, A. Basu, S. K. Bhattacharya, G. B. Nair, T. Shimada, and Y. Takeda. 1998. Development and evaluation of a multiplex PCR assay for rapid detection of toxigenic Vibrio cholerae O1 and O139. FEMS Immunol. Med. Microbiol. 20:201-207.
11. Huq, A., E. B. Small, P. A. West, M. I. Huq, R. Rahman, and R. R. Colwell. 1983. Ecological relationship between Vibrio cholerae and planktonic copepods. Appl. Environ. Microbiol. 45:275-283.
12. Huq, A., R. Sack, and R. R. Colwell. 2001. Cholera and global ecosystems, p. 327-347. In J. Aron and J. Patz (ed.), Ecosystem change and public health: a global perspective. Johns Hopkins University Press, Baltimore, Md.
13. Jesudason, M. V., V. Balaji, U. Mukudan, and C. J. Thomas. 2000. Ecological study of Vibrio cholerae in Vellore. Epidemiol. Infect. 124:201-206.
14. Jiang, S. C. 2001. Vibrio cholerae in recreational beach waters and tributaries of Southern California. Hydrobiologia 460:157-164.
15. Jiang, S. C., and W. Fu. 2001. Seasonal abundance and distribution of Vibrio cholerae in coastal waters quantified by a 16S-23S intergenic spacer probe. Microb. Ecol. 42:540-548.
16. Kaysner, C. A., C. Abeyta, Jr., M. M. Wekell, A. DePaola, Jr., R. F. Stott, and J. M. Leitch. 1987. Incidence of Vibrio cholerae from estuaries of the United States West Coast. Appl. Environ. Microbiol. 53:1344-1348.
17. Lipp, E. K., C. Rodriguez-Palacios, and J. B. Rose. 2001. Occurrence and distribution of the human pathogen Vibrio vulnificus in a subtropical Gulf of Mexico estuary. Hydrobiologia 460:165-173.
18. Louis, V., E. Russek-Cohen, N. Choopun, I. N. G. Rivera, B. Gangle, S. C. Jiang, A. Rubin, J. A. Patz, A. Huq, and R. R. Colwell. 2003. Predictability of Vibrio cholerae in Chesapeake Bay. Appl. Environ. Microbiol. 69:2773-2785.
19. Rivera, I. N. G., J. Chun, A. Huq, B. Sack, and R. R. Colwell. 2001. Genotypes associated with virulence in environmental isolates of Vibrio cholerae. Appl. Environ. Microbiol. 67:2421-2429.
20. a. Rivera, I. N. G., E. K. Lipp, N. Choopun, A. Gil, A. Huq, and R. R. Colwell. Method for DNA extraction and application of multiplex PCR to detect toxigenic V. cholerae O1 and O139 in aquatic ecosystems. Environ. Microbiol., in press.
21. Robert-Pillot, A., S. Baron, J. Lesne, J.-M. Fournier, and M.-L. Quilici. 2002. Improved detection of Vibrio cholerae in environmental water samples by culture on selective medium and colony hybridization assay with an oligonucleotide probe. FEMS Microbiol. Ecol. 40:39-46.
22. Seas, C., J. Miranda, A. I. Gil, R. Leon-Baru, J. A. Patz, A. Huq, R. R. Colwell, and R. B. Sack. 2000. New insights on the emergence of cholera in Latin America during 1991: the Peruvian experience. Am. J. Trop. Med. Hyg. 62:513-517.
23. Singh, D. V., S. R. Isac, and R. R. Colwell. 2002. Development of a hexaplex PCR assay for rapid detection of virulence and regulatory genes in Vibrio cholerae and Vibrio mimicus. J. Clin. Microbiol. 40:4321-4324.
24. Xu, H. S., N. C. Roberts, F. L. Singleton, R. W. Attwell, D. J. Grimes, and R. R. Colwell. 1982. Survival and viability of nonculturable Escherichia coli and Vibrio cholerae in the estuarine and marine environment. Microb. Ecol. 8:313-323.
25. Yamai, S., T. Okitsu, T. Shimada, and Y. Katsube. 1997. Distribution of serogroups of Vibrio cholerae non-O1 non-O139 with specific reference to their ability to produce cholera toxin, and addition of novel serogroups. J. Jpn. Assoc. Infect. Dis. 71:1037-1045.
26. Yamasaki, S., T. Shimizu, K. Hoshino, S. T. Ho, T. Shimada, G. B. Nair, and Y. Takeda. 1999. The genes responsible for O-antigen synthesis of Vibrio cholerae O139 are closely related to those of Vibrio cholerae O22. Gene 237:321-332.