Development of a hexaplex PCR assay for rapid detection of virulence and regulatory genes in Vibrio cholerae and Vibrio mimicus

Journal of Clinical Microbiology

Volumn 40, Issue 11, 2002, Pages 4321-4324

  Singh, D V ^a   Isac, Sree Renjini ^a   Colwell, R R ^a  

^a RAJIV GANDHI CENTRE FOR BIOTECHNOLOGY   (India)

Author keywords

[No Author keywords available]

Indexed keywords

CHOLERA TOXIN; OUTER MEMBRANE PROTEIN; REGULATOR PROTEIN; VIRULENCE FACTOR;

ANALYTIC METHOD; ARTICLE; BACTERIAL STRAIN; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REGULATOR GENE; SCREENING; VIBRIO CHOLERAE; VIBRIO MIMICUS;

BACTERIAL PROTEINS; CHOLERA; CHOLERA TOXIN; ENVIRONMENTAL MICROBIOLOGY; GENES, REGULATOR; HUMANS; POLYMERASE CHAIN REACTION; TIME FACTORS; VIBRIO; VIBRIO CHOLERAE; VIRULENCE;

BACTERIA (MICROORGANISMS); HEXAPLEX; NEGIBACTERIA; VIBRIO; VIBRIO CHOLERAE; VIBRIO MIMICUS;

EID: 0036840849     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.40.11.4321-4324.2002     Document Type: Article

References (34)

1. Albert, M. J., D. Islam, S. Nahar, F. Qadri, S. Falklind, and A. Weintraub. 1997. Rapid detection of Vibrio cholerae O139 Bengal from stool specimens by PCR. J. Clin. Microbiol. 35:1633-1635.
2. Colwell, R. R., J. B. Kaper, and S. W. Joseph. 1977. Vibrio cholerae, Vibrio parahaemolyticus and other vibrios: Occurrence and distribution in Chesapeake Bay. Science 198:394-396.
3. Faruque, S. M., M. J. Albert, and J. J. Mekalanos. 1998. Epidemiology, genetics, and ecology of toxigenic Vibrio cholerae. Microbiol. Mol. Biol. Rev. 62:1301-1314.
4. Fasano, A., B. Baudry, D. W. Pumplin, S. S. Wasserman, B. D. Tall, J. M. Ketley, and J. B. Kaper. 1991. Vibrio cholerae produces second enterotoxin, which affects intestinal tight junctions. Proc. Natl. Acad. Sci. USA 88:5242-5246.
5. Feinberg, A., and B. Voglestein. 1984. A technique for rapid labeling of DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 137:266-267.
6. Fields, P. I., T. Popovic, K. Wachsmuth, and Ø. Olsvik. 1992. Use of polymerase chain reaction for detection of toxigenic Vibrio cholerae O1 strains from the Latin American cholera epidemic. J. Clin. Microbiol. 30:2118-2121.
7. Herrington, D. A., R. H. Hall, G. Losonsky, J. J. Mekalanos, R. K. Taylor, and M. M. Levine. 1988. Toxin, toxin-coregulated pili and the toxR regulon are essential for Vibrio cholerae pathogenesis in humans. J. Exp. Med. 168:1487-1492.
8. Hoshino, K., S. Yamasaki, A. K. Mukhopadhyay, S. Chakraborty, A. Basu, S. K. Bhattacharya, G. B. Nair, T. Shimada, and Y. Takeda. 1998. Development and evaluation of a multiplex PCR assay for rapid detection of toxigenic Vibrio cholerae O1 and O139. FEMS Immunol. Med. Microbiol. 20:201-207.
9. Kaper, J. B., J. G. Morris, and M. M. Levine. 1995. Cholera. Clin. Microbiol. Rev. 8:48-86.
10. Kaper, J. B., S. L. Moseley, and S. Falkow. 1981. Molecular characterization of environmental and nontoxigenic strains of Vibrio cholerae. Infect. Immun. 32:661-667.
11. Keasler, S. P., and R. H. Hall. 1993. Detecting and biotyping Vibrio cholerae O1 with multiplex polymerase chain reaction. Lancet 341:1661.
12. Kim, Y., J. Okuda, C. Matsumoto, N. Takahashi, S. Hashimoto, and M. Nishibuchi. 1999. Identification of Vibrio parahaemolyticus strains at the species level by PCR targeted to the toxR gene. J. Clin. Microbiol. 37:1173-1177.
13. Kurazono, H., A. Pal, P. K. Bag, G. B. Nair, T. Karasawa, T. Mihara, and Y. Takeda. 1995. Distribution of genes encoding cholera toxin, zonula occludens toxin, accessory cholera enterotoxin, and El Tor hemolysin in Vibrio cholerae of diverse origins. Microb. Pathol. 18:231-235.
14. Levine, M. M., R. E. Black, M. L. Clements, L. Cisnors, A. Saah, D. R. Nalin, D. M. Gill, J. P. Craig, C. R. Young, and P. Ristaino. 1982. The pathogenicity of nonenterotoxigenic Vibrio cholerae serogroup O1 biotype El Tor isolated from sewage water in Brazil. J. Infect. Dis. 145:296-299.
15. Lin, Z., K. Kumagi, K. Baba, J. J. Mekalanos, and M. Nishibuchi. 1993. Vibrio parahaemolyticus has a homologue of the Vibrio cholerae toxRS operon that mediates environmentally induced regulation of the thermostable direct hemolysin gene. J. Bacteriol. 175:3849-3855.
16. Maniatis, T., E. F. Fritsch, and J. Sambrook. 1982. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
17. Miller, V. L., R. K. Taylor, and J. J. Mekalanos. 1987. Cholera toxin transcriptional activator ToxR is a transmembrane DNA binding protein. Cell 48:271-279.
18. Miller, V. L., and J. J. Mekalanos. 1988. A novel suicide vector and its use in construction of insertion mutations: Osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR. J. Bacteriol. 170:2575-2583.
19. Naksone, N., and M. Iwanaga. 1998. Characterization of outer membrane protein OmpU of Vibrio cholerae O1. Infect. Immun. 66:4726-4728.
20. Nandi, B., R. K. Nandi, S. Mukhopadhyay, G. B. Nair, T. Shimada, and A. C. Ghose. 2000. Rapid detection of species-specific identification of Vibrio cholerae with primers targeted to the gene of outer membrane protein OmpW. J. Clin. Microbiol. 38:4145-4151.
21. Osorio, C. R., and K. E. Klose. 2000. A region of transmembrane regulatory protein ToxR that tethers the transcriptional activation domain to the cytoplasmic membrane displays wide divergence among Vibrio species. J. Bacteriol. 182:526-528.
22. Provenzano, D., and K. E. Klose. 2000. Altered expression of toxR-regulated porins OmpU and OmpT diminishes Vibrio cholerae bile resistance virulence factor expression, and intestinal colonization. Proc. Natl. Acad. Sci. USA 97:10220-10224.
23. Provenzano, D., C. M. Lauriano, and K. E. Klose. 2001. Characterization of the role of toxR-modulated outer membrane porins OmpU and OmpT in Vibrio cholerae virulence. J. Bacteriol. 183:3652-3662.
24. Reich, K. A., and G. K. Schoolnik. 1994. The light organ symbiont Vibrio fisheri possesses a homologue of the Vibrio cholerae transmembrane transcriptional activator ToxR. J. Bacteriol. 176:3085-3088.
25. Rivera, I. N. G., J. Chun, A. Huq, R. B. Sack, and R. R. Colwell. 2001. Genotype associated with virulence in environmental isolates of Vibrio cholerae. Appl. Environ. Microbiol. 67:2421-2429.
26. Sakazaki, R. 1992. Bacteriology of Vibrio and related organisms, p. 37-55. In D. Barua and W. B. Greenough III (ed.), Cholera. Plenum Medical Book Company, New York, N.Y.
27. Sanyal, S. C., M. I. Huq, P. K. B. Neogy, K. Alam, M. I. Kabir, and A. S. M. H. Rahman. 1983. Vibrio mimicus as an aetiologic agent of diarrhoea and its pathogenesis. Indian J. Med. Microbiol. 1:1-12.
28. Shi, L., S. Miyoshi, M. Hiura, K. Tomochika, T. Shimada, and S. Shinoda. 1998. Detection of genes encoding cholera toxin (cholera toxin), zonula occludens toxin (ZOT), accessory cholera enterotoxin (ACE) and heat stable enterotoxin (ST) in Vibrio mimicus clinical strains. Microbiol. Immunol. 42:823-828.
29. Shirai, H., M. Nishibuchi, T. Ramamurthy, S. K. Bhattacharya, S. C. Pal, and Y. Takeda. 1991. Polymerase chain reaction of the cholera enterotoxin operon of Vibrio cholerae. J. Clin. Microbiol. 29:2517-2521.
30. Singh, D. V., M. H. Matte, G. R. Matte, S. Jiang, F. Sabeena, B. N. Shukla, S. C. Sanyal, A. Huq, and R. R. Colwell. 2001. Molecular analysis of Vibrio cholerae O1, O139, non-O1, and non-O139 strains: Clonal relationships between clinical and environmental isolates. Appl. Environ. Microbiol. 67:910-921.
31. Taylor, R. K., C. Shaw, K. Peterson, P. Spears, and J. J. Mekalanos. 1987. Safe, live Vibrio cholerae vaccines? Vaccine 6:151-154.
32. Taylor, R. K., V. L. Miller, D. B. Furlong, and J. J. Mekalanos. 1987. Use of phoA gene fusion to identify a pilus colonization factor coordinately regulated with cholera toxin. Proc. Natl. Acad. Sci. USA 84:2833-2837.
33. Trucksis, M., J. E. Galen, J. Michalski, A. Fasano, and J. B. Kaper. 1993. Accessory cholera enterotoxin (Ace), the third toxin of a Vibrio cholerae virulence cassette. Proc. Natl. Acad. Sci. USA 90:5267-5271.
34. World Health Organization. 1987. Manual for Laboratory Investigation of Acute Infection. Publication W.H.O. CDD/83.3. Program for Control of Diarrhoeal Diseases. World Health Organization, Geneva, Switzerland.