Molecular cloning and characterization of a new cold-active esterase from a deep-sea metagenomic library

Applied Microbiology and Biotechnology

Volumn 90, Issue 3, 2011, Pages 961-970

  Fu, Chengzhang ^a,c   Hu, Yongfei ^a,c   Xie, Feng ^a,c   Guo, Hui ^a,c   Ashforth, Elizabeth Jane ^a   Polyak, Steven W ^d   Zhu, Baoli ^a   Zhang, Lixin ^a,b  

^a INSTITUTE OF MICROBIOLOGY   (China)

^b SOUTH CHINA SEA INSTITUTE OF OCEANOLOGY   (China)

^c UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

^d UNIVERSITY OF ADELAIDE   (Australia)

Author keywords

Cold active; Esterase; Marine sediment; Metagenome

Indexed keywords

AMINO ACID RESIDUES; AMINO ACID SEQUENCE; BIOCHEMICAL PROPERTIES; COLD-ACTIVE; ESTERASE; LIPOLYTIC ACTIVITY; LIPOLYTIC ENZYMES; LOW TEMPERATURES; MARINE SEDIMENTS; METAGENOMES; METAGENOMIC LIBRARIES; MOLECULAR CLONING; N-TERMINALS; PH RANGE; PHYLOGENETIC ANALYSIS; PHYLOGENETIC TREES; SIGNAL PEPTIDE; SOUTH CHINA SEA; TEMPERATURE RANGE;

AMINO ACIDS; ANOXIC SEDIMENTS; CATALYSTS; CLONING; ESTERS; GENES; SEDIMENTOLOGY; SUBMARINE GEOLOGY; SUBSTRATES;

ENZYMES;

ESTERASE; RECOMBINANT ENZYME;

AMINO ACID; CLONE; DEEP SEA; ENZYME ACTIVITY; GENE EXPRESSION; GENOMICS; HYDROLYSIS; HYPOTHESIS TESTING; LOW TEMPERATURE; MARINE SEDIMENT; MOLECULAR ANALYSIS; PEPTIDE; PH; PHYLOGENETICS;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BIOCHEMISTRY; ENZYME ACTIVITY; ENZYME PURIFICATION; GENE LIBRARY; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; PROTEIN PURIFICATION; UNINDEXED SEQUENCE;

AMINO ACID SEQUENCE; BACTERIA; BACTERIAL PROTEINS; CLONING, MOLECULAR; COLD TEMPERATURE; ENZYME STABILITY; ESTERASES; GENOMIC LIBRARY; GEOLOGIC SEDIMENTS; KINETICS; METAGENOMICS; MOLECULAR SEQUENCE DATA; PHYLOGENY; SEAWATER; SEQUENCE ALIGNMENT;

EID: 79954906539     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-010-3079-0     Document Type: Article

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