HIV-1 integrase modulates the interaction of the HIV-1 cellular cofactor LEDGF/p75 with chromatin

Retrovirology

Volumn 8, Issue , 2011, Pages

  Astiazaran, Paulina ^a   Bueno, Murilo T D ^a   Morales, Elisa ^a   Kugelman, Jeffrey R ^a   Garcia Rivera, Jose A ^a   Llano, Manuel ^a  

^a UNIVERSITY OF TEXAS AT EL PASO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

INTEGRASE; LENS EPITHELIUM DERIVED GROWTH FACTOR; PROTEIN P75; LENS EPITHELIUM-DERIVED GROWTH FACTOR; MUTANT PROTEIN; P31 INTEGRASE PROTEIN, HUMAN IMMUNODEFICIENCY VIRUS 1; SIGNAL PEPTIDE;

ARTICLE; CD4+ T LYMPHOCYTE; CELL CYCLE; CHROMATIN; CONTROLLED STUDY; GENETIC TRANSDUCTION; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS 1 INFECTION; IMMUNOPRECIPITATION; IONIC STRENGTH; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; PROTEIN STABILITY; CELL LINE; GENE DELETION; GENETICS; METABOLISM; PHYSIOLOGY; PROTEIN ANALYSIS; VIRUS DNA CELL DNA INTERACTION;

HUMAN IMMUNODEFICIENCY VIRUS 1;

CELL LINE; CHROMATIN; HIV INTEGRASE; HIV-1; HUMANS; INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS; MUTANT PROTEINS; PROTEIN BINDING; PROTEIN INTERACTION DOMAINS AND MOTIFS; PROTEIN INTERACTION MAPPING; SEQUENCE DELETION; VIRUS INTEGRATION;

EID: 79954622519     PISSN: None     EISSN: 17424690     Source Type: Journal    
DOI: 10.1186/1742-4690-8-27     Document Type: Article

References (33)

1. Llano M, Saenz DT, Meehan A, Wongthida P, Peretz M, Walker WH, Teo W, Poeschla EM. An essential role for LEDGF/p75 in HIV integration. Science 2006, 314:461-464. 10.1126/science.1132319, 16959972.
2. Shun MC, Raghavendra NK, Vandegraaff N, Daigle JE, Hughes S, Kellam P, Cherepanov P, Engelman A. LEDGF/p75 functions downstream from preintegration complex formation to effect gene-specific HIV-1 integration. Genes Dev 2007, 21:1767-1778. 10.1101/gad.1565107, 1920171, 17639082.
3. Vandekerckhove L, Christ F, Van Maele B, De Rijck J, Gijsbers R, Van den Haute C, Witvrouw M, Debyser Z. Transient and stable knockdown of the integrase cofactor LEDGF/p75 reveals its role in the replication cycle of human immunodeficiency virus. J Virol 2006, 80:1886-1896. 10.1128/JVI.80.4.1886-1896.2006, 1367129, 16439544.
4. Yokoyama A, Cleary ML. Menin critically links MLL proteins with LEDGF on cancer-associated target genes. Cancer Cell 2008, 14:36-46. 10.1016/j.ccr.2008.05.003, 2692591, 18598942.
5. Ferris AL, Wu X, Hughes CM, Stewart C, Smith SJ, Milne TA, Wang GG, Shun MC, Allis CD, Engelman A, Hughes SH. Lens epithelium-derived growth factor fusion proteins redirect HIV-1 DNA integration. Proc Natl Acad Sci USA 2010, 107:3135-3140. 10.1073/pnas.0914142107, 2840313, 20133638.
6. Gijsbers R, Ronen K, Vets S, Malani N, De Rijck J, McNeely M, Bushman FD, Debyser Z. LEDGF hybrids efficiently retarget lentiviral integration into heterochromatin. Mol Ther 18:552-560.
7. Meehan AM, Saenz DT, Morrison JH, Garcia-Rivera JA, Peretz M, Llano M, Poeschla EM. LEDGF/p75 proteins with alternative chromatin tethers are functional HIV-1 cofactors. PLoS Pathog 2009, 5:e1000522. 10.1371/journal.ppat.1000522, 2706977, 19609362.
8. Llano M, Vanegas M, Hutchins N, Thompson D, Delgado S, Poeschla EM. Identification and characterization of the chromatin-binding domains of the HIV-1 integrase interactor LEDGF/p75. J Mol Biol 2006, 360:760-773. 10.1016/j.jmb.2006.04.073, 16793062.
9. Maertens G, Cherepanov P, Pluymers W, Busschots K, De Clercq E, Debyser Z, Engelborghs Y. LEDGF/p75 is essential for nuclear and chromosomal targeting of HIV-1 integrase in human cells. J Biol Chem 2003, 278:33528-33539. 10.1074/jbc.M303594200, 12796494.
10. Nishizawa Y, Usukura J, Singh DP, Chylack LT, Shinohara T. Spatial and temporal dynamics of two alternatively spliced regulatory factors, lens epithelium-derived growth factor (ledgf/p75) and p52, in the nucleus. Cell Tissue Res 2001, 305:107-114. 10.1007/s004410100398, 11512661.
11. Turlure F, Maertens G, Rahman S, Cherepanov P, Engelman A. A tripartite DNA-binding element, comprised of the nuclear localization signal and two AT-hook motifs, mediates the association of LEDGF/p75 with chromatin in vivo. Nucleic Acids Res 2006, 34:1653-1675. 10.1093/nar/gkl052, 1405818, 16549878.
12. Vanegas M, Llano M, Delgado S, Thompson D, Peretz M, Poeschla E. Identification of the LEDGF/p75 HIV-1 integrase-interaction domain and NLS reveals NLS-independent chromatin tethering. J Cell Sci 2005, 118:1733-1743. 10.1242/jcs.02299, 15797927.
13. Garcia-Rivera JA, Bueno MT, Morales E, Kugelman JR, Rodriguez DF, Llano M. Implication of serine residues 271, 273, and 275 in the human immunodeficiency virus type 1 cofactor activity of lens epithelium-derived growth factor/p75. J Virol 2010, 84:740-752. 10.1128/JVI.01043-09, 2798348, 19889764.
14. Botbol Y, Raghavendra NK, Rahman S, Engelman A, Lavigne M. Chromatinized templates reveal the requirement for the LEDGF/p75 PWWP domain during HIV-1 integration in vitro. Nucleic Acids Res 2008, 36:1237-1246. 2275106, 18174227.
15. Shun MC, Botbol Y, Li X, Di Nunzio F, Daigle JE, Yan N, Lieberman J, Lavigne M, Engelman A. Identification and characterization of PWWP domain residues critical for LEDGF/p75 chromatin binding and human immunodeficiency virus type 1 infectivity. J Virol 2008, 82:11555-11567. 10.1128/JVI.01561-08, 2583668, 18799576.
16. Chiu TK, Davies DR. Structure and function of HIV-1 integrase. Curr Top Med Chem 2004, 4:965-977. 10.2174/1568026043388547, 15134551.
17. Delelis O, Carayon K, Saib A, Deprez E, Mouscadet JF. Integrase and integration: biochemical activities of HIV-1 integrase. Retrovirology 2008, 5:114. 10.1186/1742-4690-5-114, 2615046, 19091057.
18. Busschots K, Vercammen J, Emiliani S, Benarous R, Engelborghs Y, Christ F, Debyser Z. The interaction of LEDGF/p75 with integrase is lentivirus-specific and promotes DNA binding. J Biol Chem 2005, 280:17841-17847.
19. Llano M, Morrison J, Poeschla EM. Virological and cellular roles of the transcriptional coactivator LEDGF/p75. Curr Top Microbiol Immunol 2009, 339:125-146. 10.1007/978-3-642-02175-6_7, 20012527.
20. Engelman A, Cherepanov P. The lentiviral integrase binding protein LEDGF/p75 and HIV-1 replication. PLoS Pathog 2008, 4:e1000046. 10.1371/journal.ppat.1000046, 2275779, 18369482.
21. Poeschla EM. Integrase, LEDGF/p75 and HIV replication. Cell Mol Life Sci 2008,
22. Llano M, Vanegas M, Fregoso O, Saenz D, Chung S, Peretz M, Poeschla EM. LEDGF/p75 determines cellular trafficking of diverse lentiviral but not murine oncoretroviral integrase proteins and is a component of functional lentiviral preintegration complexes. J Virol 2004, 78:9524-9537. 10.1128/JVI.78.17.9524-9537.2004, 506940, 15308744.
23. Emiliani S, Mousnier A, Busschots K, Maroun M, Van Maele B, Tempe D, Vandekerckhove L, Moisant F, Ben-Slama L, Witvrouw M, et al. Integrase mutants defective for interaction with LEDGF/p75 are impaired in chromosome tethering and HIV-1 replication. J Biol Chem 2005, 280:25517-25523. 10.1074/jbc.M501378200, 15855167.
24. Rahman S, Lu R, Vandegraaff N, Cherepanov P, Engelman A. Structure-based mutagenesis of the integrase-LEDGF/p75 interface uncouples a strict correlation between in vitro protein binding and HIV-1 fitness. Virology 2007, 357:79-90. 10.1016/j.virol.2006.08.011, 16959283.
25. Maertens G, Vercammen J, Debyser Z, Engelborghs Y. Measuring protein-protein interactions inside living cells using single color fluorescence correlation spectroscopy. Application to human immunodeficiency virus type 1 integrase and LEDGF/p75. Faseb J 2005, 19:1039-1041.
26. Hendrix J, Gijsbers R, De Rijck J, Voet AHJ, McNeely M, Hofkens J, Debyser ZY. The transcriptional co-activator LEDGF/p75 displays a dynamic scan-and-lock mechanism for chromatin tethering. Nucleic Acids Res 2010,
27. Maertens G, Cherepanov P, Debyser Z, Engelborghs Y, Engelman A. Identification and characterization of a functional nuclear localization signal in the HIV-1 integrase interactor LEDGF/p75. J Biol Chem 2004, 279:33421-33429. 10.1074/jbc.M404700200, 15163664.
28. Meehan AM, Saenz DT, Morrison J, Hu C, Peretz M, Poeschla EM. LEDGF dominant interference proteins demonstrate pre-nuclear exposure of HIV-1 integrase and synergize with LEDGF depletion to destroy viral infectivity. J Virol 2011,
29. De Rijck J, Vandekerckhove L, Gijsbers R, Hombrouck A, Hendrix J, Vercammen J, Engelborghs Y, Christ F, Debyser Z. Overexpression of the lens epithelium-derived growth factor/p75 integrase binding domain inhibits human immunodeficiency virus replication. J Virol 2006, 80:11498-11509. 10.1128/JVI.00801-06, 1642583, 16987986.
30. Bartholomeeusen K, De Rijck J, Busschots K, Desender L, Gijsbers R, Emiliani S, Benarous R, Debyser Z, Christ F. Differential interaction of HIV-1 integrase and JPO2 with the C terminus of LEDGF/p75. J Mol Biol 2007, 372:407-421. 10.1016/j.jmb.2007.06.090, 17669426.
31. Maertens GN, Cherepanov P, Engelman A. Transcriptional co-activator p75 binds and tethers the Myc-interacting protein JPO2 to chromatin. J Cell Sci 2006, 119:2563-2571. 10.1242/jcs.02995, 16735438.
32. Bartholomeeusen K, Gijsbers R, Christ F, Hendrix J, Rain JC, Emiliani S, Benarous R, Debyser Z, De Rijck J. Lens Epithelium Derived Growth Factor/p75 interacts with the transposase derived DDE domain of pogZ. J Biol Chem 2009,
33. Hughes S, Jenkins V, Dar MJ, Engelman A, Cherepanov P. Transcriptional co-activator LEDGF interacts with Cdc7-activator of S-phase kinase (ASK) and stimulates its enzymatic activity. J Biol Chem 2010, 285:541-554. 10.1074/jbc.M109.036491, 2804203, 19864417.