Lens epithelium-derived growth factor fusion proteins redirect HIV-1 DNA integration

Proceedings of the National Academy of Sciences of the United States of America

Volumn 107, Issue 7, 2010, Pages 3135-3140

  Ferris, Andrea L ^a   Wu, Xiaolin ^b   Hughes, Christina M ^c   Stewart, Claudia ^b   Smith, Steven J ^a   Milne, Thomas A ^c   Wang, Gang G ^c   Shun, Ming Chieh ^d   Allis, C David ^c   Engelman, Alan ^d   Hughes, Stephen H ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

^b SAIC FREDERICK INC   (United States)

^c ROCKEFELLER UNIVERSITY   (United States)

^d DANA FARBER CANCER INSTITUTE   (United States)

Author keywords

Chromatin; Histone marks; Lentiviral vectors

Indexed keywords

HETEROCHROMATIN PROTEIN 1; HISTONE H3; HISTONE H3K4ME3; HOMEODOMAIN PROTEIN; HYBRID PROTEIN; LENS EPITHELIUM DERIVED GROWTH FACTOR; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CHROMATIN; CONTROLLED STUDY; DNA INTEGRATION; EMBRYO; GENE EXPRESSION; GENOME; HETEROCHROMATIN; HUMAN IMMUNODEFICIENCY VIRUS 1; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN LOCALIZATION; TRANSCRIPTION INITIATION;

ANIMALS; BINDING SITES; CELL LINE; CHROMATIN; COMPUTATIONAL BIOLOGY; DNA, VIRAL; FLOW CYTOMETRY; GENE EXPRESSION PROFILING; GENE THERAPY; HIV INTEGRASE; HIV-1; HOMEODOMAIN PROTEINS; INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS; MICE; MICE, KNOCKOUT; PROTEIN STRUCTURE, TERTIARY; SEQUENCE ANALYSIS, DNA; TUMOR SUPPRESSOR PROTEINS; VIRUS INTEGRATION;

HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 77649259825     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.0914142107     Document Type: Article

References (34)

1. Hughes SH, et al. (1978) Proviruses of avian sarcoma virus are terminally redundant, co-extensive with unintegrated linear DNA and integrated at many sites. Cell 15: 1397-1410.
2. Derse D, et al. (2007) Human T-cell leukemia virus type 1 integration target sites in the human genome: comparison with those of other retroviruses. J Virol 81:6731-6741.
3. Lewinski MK, et al. (2006) Retroviral DNA integration: viral and cellular determinants of target-site selection. PLoS Pathog 2:e60.
4. Wu X, Li Y, Crise B, Burgess SM (2003) Transcription start regions in the human genome are favored targets for MLV integration. Science 300:1749-1751.
5. Mitchell RS, et al. (2004) Retroviral DNA integration: ASLV, HIV, and MLV show distinct target site preferences. PLoS Biol 2:E234.
6. Wang GP, Ciuffi A, Leipzig J, Berry CC, Bushman FD (2007) HIV integration site selection: Analysis by massively parallel pyrosequencing reveals association with epigenetic modifications. Genome Res 17:1186-1194.
7. Narezkina A, et al. (2004) Genome-wide analyses of avian sarcoma virus integration sites. J Virol 78:11656-11663.
8. Marshall HM, et al. (2007) Role of PSIP1/LEDGF/p75 in lentiviral infectivity and integration targeting. PLoS One 2:e1340.
9. Schröder AR, et al. (2002) HIV-1 integration in the human genome favors active genes and local hotspots. Cell 110:521-529.
10. Dai J, Xie W, Brady TL, Gao J, Voytas DF (2007) Phosphorylation regulates integration of the yeast Ty5 retrotransposon into heterochromatin. Mol Cell 27:289-299.
11. Wang H, Heinz ME, Crosby SD, Johnston M, Mitra RD (2008) 'Calling Cards' method for high-throughput identification of targets of yeast DNA-binding proteins. Nat Protoc 3:1569-1577.
12. Leem YE, et al. (2008) Retrotransposon Tf1 is targeted to Pol II promoters by transcription activators. Mol Cell 30:98-107.
13. Hare S, et al. (2009) A novel co-crystal structure affords the design of gain-of-function lentiviral integrase mutants in the presence of modified PSIP1/LEDGF/p75. PLoS Pathog 5:e1000259.
14. Turlure F, Maertens G, Rahman S, Cherepanov P, Engelman A (2006) A tripartite DNA-binding element, comprised of the nuclear localization signal and two AT-hook motifs, mediates the association of LEDGF/p75 with chromatin in vivo. Nucleic Acids Res 34:1653-1665.
15. Llano M, et al. (2006) Identification and characterization of the chromatin-binding domains of the HIV-1 integrase interactor LEDGF/p75. J Mol Biol 360:760-773.
16. Shun MC, et al. (2008) Identification and characterization of PWWP domain residues critical for LEDGF/p75 chromatin binding and human immunodeficiency virus type 1 infectivity. J Virol 82:11555-11567.
17. Poeschla EM (2008) Integrase, LEDGF/p75 and HIV replication. Cell Mol Life Sci 65: 1403-1424.
18. Llano M, et al. (2006) An essential role for LEDGF/p75 in HIV integration. Science 314: 461-464.
19. Shun MC, et al. (2007) LEDGF/p75 functions downstream from preintegration complex formation to effect gene-specific HIV-1 integration. Genes Dev 21:1767-1778.
20. Engelman A, Cherepanov P (2008) The lentiviral integrase binding protein LEDGF/p75 and HIV-1 replication. PLoS Pathog 4:e1000046.
21. Vandekerckhove L, et al. (2006) Transient and stable knockdown of the integrase cofactor LEDGF/p75 reveals its role in the replication cycle of human immunodeficiency virus. J Virol 80:1886-1896.
22. Vandegraaff N, Devroe E, Turlure F, Silver PA, Engelman A (2006) Biochemical and genetic analyses of integrase-interacting proteins lens epithelium-derived growth factor (LEDGF)/p75 and hepatoma-derived growth factor related protein 2 (HRP2) in preintegration complex function and HIV-1 replication. Virology 346:415-426.
23. Meehan AM, et al. (2009) LEDGF/p75 proteins with alternative chromatin tethers are functional HIV-1 cofactors. PLoS Pathog 5:e1000522.
24. Hacein-Bey-Abina S, et al. (2003) LMO2-associated clonal T cell proliferation in two patients after gene therapy for SCID-X1. Science 302:415-419.
25. Wysocka J, et al. (2006) A PHD finger of NURF couples histone H3 lysine 4 trimethylation with chromatin remodelling. Nature 442:86-90.
26. Ruthenburg AJ, Allis CD, Wysocka J (2007) Methylation of lysine 4 on histone H3: Intricacy of writing and reading a single epigenetic mark. Mol Cell 25:15-30.
27. Vakoc CR, Mandat SA, Olenchock BA, Blobel GA (2005) Histone H3 lysine 9 methylation and HP1gamma are associated with transcription elongation through mammalian chromatin. Mol Cell 19:381-391.
28. Holman AG, Coffin JM (2005) Symmetrical base preferences surrounding HIV-1, avian sarcoma/leukosis virus, and murine leukemia virus integration sites. Proc Natl Acad Sci USA 102:6103-6107.
29. Wu X, Li Y, Crise B, Burgess SM, Munroe DJ (2005) Weak palindromic consensus sequences are a common feature found at the integration target sites of many retroviruses. J Virol 79:5211-5214.
30. Barski A, et al. (2007) High-resolution profiling of histone methylations in the human genome. Cell 129:823-837.
31. Julias JG, Ferris AL, Boyer PL, Hughes SH (2001) Replication of phenotypically mixed human immunodeficiency virus type 1 virions containing catalytically active and catalytically inactive reverse transcriptase. J Virol 75:6537-6546.
32. Thomas JA, Ott DE, Gorelick RJ (2007) Efficiency of human immunodeficiency virus type 1 postentry infection processes: evidence against disproportionate numbers of defective virions. J Virol 81:4367-4370.
33. He J, Landau NR (1995) Use of a novel human immunodeficiency virus type 1 reporter virus expressing human placental alkaline phosphatase to detect an alternative viral receptor. J Virol 69:4587-4592.
34. Hirt B (1967) Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol 26:365-369.