The Ndc80 complex uses a tripartite attachment point to couple microtubule depolymerization to chromosome movement

Molecular Biology of the Cell

Volumn 22, Issue 8, 2011, Pages 1217-1226

  Tooley, John G ^a   Miller, Stephanie A ^a   Stukenberg, P Todd ^a  

^a UNIVERSITY OF VIRGINIA MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; CALPONIN; CELL PROTEIN; MUTANT PROTEIN; NDC80 COMPLEX; PROTEIN HEC1; SPINDLE CHECKPOINT PROTEIN; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; BINDING AFFINITY; BINDING KINETICS; BINDING SITE; CENTROMERE; CHROMOSOME; CHROMOSOME MOVEMENT; CONTROLLED STUDY; DEPOLYMERIZATION; HUMAN; HUMAN CELL; IN VITRO STUDY; MICROTUBULE; MICROTUBULE ASSEMBLY; MITOSIS SPINDLE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; REGULATORY MECHANISM;

ANIMALS; CALCIUM-BINDING PROTEINS; CELL CYCLE; CHROMOSOME SEGREGATION; CIRCULAR DICHROISM; FEMALE; GENE SILENCING; HELA CELLS; HUMANS; KINETOCHORES; MICROFILAMENT PROTEINS; MICROTUBULES; MITOSIS; MITOTIC SPINDLE APPARATUS; MODELS, MOLECULAR; MUTATION; NUCLEAR PROTEINS; POLYMERIZATION; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; PROTEIN-SERINE-THREONINE KINASES; RNA, SMALL INTERFERING; TRANSFECTION; VERTEBRATES;

VERTEBRATA;

EID: 79954598474     PISSN: 10591524     EISSN: 19394586     Source Type: Journal    
DOI: 10.1091/mbc.E10-07-0626     Document Type: Article

References (33)

1. Alushin GM, Ramey VH, Pasqualato S, Ball DA, Grigorieff N, Musacchio A, Nogales E (2010). The Ndc80 kinetochore complex forms oligomeric arrays along microtubules. Nature 467, 805-810.
2. Brinkley BR, Cartwright J Jr (1975). Cold-labile and cold-stable microtubules in the mitotic spindle of mammalian cells. Ann N Y Acad Sci 253, 428-439.
3. Cheeseman IM, Chappie JS, Wilson-Kubalek EM, Desai A (2006). The conserved KMN network constitutes the core microtubule-binding site of the kinetochore. Cell 127, 983-997. (Pubitemid 44792248)
4. Cheeseman IM, Desai A (2008). Molular architecture of the kinetochore-microtubule interface. Nat Rev Mol Cell Biol 9, 33-46.
5. Ciferri C et al. (2008). Implications for kinetochore-microtubule attachment from the structure of an engineered Ndc80 complex. Cell 133, 427-439. (Pubitemid 351622013)
6. Cleveland DW, Mao Y, Sullivan KF (2003). Centromeres and kinetochores: From epigenetics to mitotic checkpoint signaling. Cell 112, 407-421. (Pubitemid 36263076)
7. Deluca JG, Gall WE, Ciferri C, Cimini D, Musacchio A, Salmon ED (2006). Kinetochore microtubule dynamics and attachment stability are regulated by Hec1. Cell 127, 969-982. (Pubitemid 44792254)
8. Deluca JG, Howell BJ, Canman JC, Hickey JM, Fang G, Salmon ED (2003). Nuf2 and Hec1 are required for retention of the checkpoint proteins Mad1 and Mad2 to kinetochores. Curr Biol 13, 2103-2109. (Pubitemid 37502528)
9. Deluca JG, Moree B, Hickey JM, Kilmartin JV, Salmon ED (2002). hNuf2 inhibition blocks stable kinetochore-microtubule attachment and induces mitotic cell death in HeLa cells. J Cell Biol 159, 549-555.
10. Derewenda U et al. (2007). The structure of the coiled-coil domain of Ndel1 and the basis of its interaction with Lis1, the causal protein of Miller-Dieker lissencephaly. Structure 15, 1467-1481. (Pubitemid 350064493)
11. Desai A, Murray A, Mitchison TJ, Walczak CE (1999). The use of Xenopus egg extracts to study mitotic spindle assembly and function in vitro. Methods Cell Biol 61, 385-412.
12. Du J et al. (2008). The mitotic checkpoint kinase NEK2A regulates kinetochore microtubule attachment stability. Oncogene 27, 4107-4114. (Pubitemid 351931984)
13. Guimaraes GJ, Dong Y, McEwen BF, Deluca JG (2008). Kinetochore- microtubule attachment relies on the disordered N-terminal tail domain of Hec1. Curr Biol 18, 1778-1784.
14. Hayashi I, Ikura M (2003). Crystal structure of the amino-terminal microtubule-binding domain of end-binding protein 1 (EB1). J Biol Chem 278, 36430-36434. (Pubitemid 37139972)
15. Hiser L, Aggarwal A, Young R, Frankfurter A, Spano A, Correia JJ, Lobert S (2006). Comparison of beta-tubulin mRNA and protein levels in 12 human cancer cell lines. Cell Motil Cytoskeleton 63, 41-52. (Pubitemid 43157644)
16. Hoffman DB, Pearson CG, Yen TJ, Howell BJ, Salmon ED (2001). Microtubule-dependent changes in assembly of microtubule motor proteins and mitotic spindle checkpoint proteins at PtK1 kinetochores. Mol Biol Cell 12, 1995-2009. (Pubitemid 33049694)
17. Maddox P, Straight A, Coughlin P, Mitchison TJ, Salmon ED (2003). Direct observation of microtubule dynamics at kinetochores in Xenopus extract spindles: Implications for spindle mechanics. J Cell Biol 162, 377-382. (Pubitemid 36988547)
18. Maiolica A, Cittaro D, Borsotti D, Sennels L, Ciferri C, Tarricone C, Musacchio A, Rappsilber J (2007). Structural analysis of multiprotein complexes by cross-linking, mass spectrometry, and database searching. Mol Cell Proteomics 6, 2200-2211.
19. Martin-Lluesma S, Stucke VM, Nigg EA (2002). Role of Hec1 in spindle checkpoint signaling and kinetochore recruitment of Mad1/Mad2. Science 297, 2267-2270.
20. McCleland ML, Gardner RD, Kallio MJ, Daum JR, Gorbsky GJ, Burke DJ, Stukenberg PT (2003). The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity. Genes Dev 17, 101-114. (Pubitemid 36062512)
21. McCleland ML, Kallio MJ, Barrett-Wilt GA, Kestner CA, Shabanowitz J, Hunt DF, Gorbsky GJ, Stukenberg PT (2004). The vertebrate Ndc80 complex contains Spc24 and Spc25 homologs, which are required to establish and maintain kinetochore-microtubule attachment. Curr Biol 14, 131-137. (Pubitemid 38203926)
22. Miller SA, Johnson ML, Stukenberg PT (2008). Kinetochore attachments require an interaction between unstructured tails on microtubules and Ndc80(Hec1). Curr Biol 18, 1785-1791.
23. Powers AF, Franck AD, Gestaut DR, Cooper J, Gracyzk B, Wei RR, Wordeman L, Davis TN, Asbury CL (2009). The Ndc80 kinetochore complex forms load-bearing attachments to dynamic microtubule tips via biased diffusion. Cell 136, 865-875.
24. Santaguida S, Musacchio A (2009). The life and miracles of kinetochores. EMBO J 28, 2511-2531.
25. Sjoblom B, Ylanne J, Djinovic-Carugo K (2008). Novel structural insights into F-actin-binding and novel functions of calponin homology domains. Curr Opin Struct Biol 18, 702-708.
26. Slep KC, Vale RD (2007). Structural basis of microtubule plus end tracking by XMAP215, CLIP-170, and EB1. Mol Cell 27, 976-991. (Pubitemid 47488185)
27. Wan X et al. (2009). Protein architecture of the human kinetochore microtubule attachment site. Cell 137, 672-684.
28. Wei RR, Al Bassam J, Harrison SC (2007). The Ndc80/HEC1 complex is a contact point for kinetochore-microtubule attachment. Nat Struct Mol Biol 14, 54-59. (Pubitemid 46067423)
29. Wei RR, Schnell JR, Larsen NA, Sorger PK, Chou JJ, Harrison SC (2006). Structure of a central component of the yeast kinetochore: The Spc24p/Spc25p globular domain. Structure 14, 1003-1009. (Pubitemid 43831664)
30. Wei RR, Sorger PK, Harrison SC (2005). Molecular organization of the Ndc80 complex, an essential kinetochore component. Proc Natl Acad Sci USA 102, 5363-5367. (Pubitemid 40530263)
31. Welburn JP, Vleugel M, Liu D, Yates JR III, Lampson MA, Fukagawa T, Cheeseman IM (2010). Aurora B phosphorylates spatially distinct targets to differentially regulate the kinetochore-microtubule interface. Mol Cell 38, 383-392.
32. Wigge PA, Kilmartin JV (2001). The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation. J Cell Biol 152, 349-360. (Pubitemid 34285604)
33. Zimniak T, Stengl K, Mechtler K, Westermann S (2009). Phosphoregulation of the budding yeast EB1 homologue Bim1p by Aurora/Ipl1p. J Cell Biol 186, 379-391.