The effect of ex vivo dynamic loading on the osteogenic differentiation of genetically engineered mesenchymal stem cell model

Journal of Tissue Engineering and Regenerative Medicine

Volumn 5, Issue 5, 2011, Pages 384-393

  Kimelman Bleich, Nadav ^a   Seliktar, Dror ^b   Kallai, Ilan ^a   Helm, Gregory A ^c   Gazit, Zulma ^a,d   Gazit, Dan ^a,d   Pelled, Gadi ^a,d  

^a HEBREW UNIVERSITY OF JERUSALEM   (Israel)

^b TECHNION ISRAEL INSTITUTE OF TECHNOLOGY   (Israel)

^c UNIVERSITY OF VIRGINIA HEALTH SYSTEM   (United States)

^d CEDARS SINAI MEDICAL CENTER   (United States)

Author keywords

Bioreactors; Bone; Mechanical effects on cells and tissues; Mesenchymal stem cells

Indexed keywords

BIOREACTORS; BONE; DYNAMIC MECHANICAL ANALYSIS; DYNAMICS; FLOWCHARTING; HYDROGELS; METABOLISM; PHOSPHATASES; RECOMBINANT PROTEINS; STEM CELLS;

ALKALINE PHOSPHATASE ACTIVITY; DYNAMIC LOADINGS; EX-VIVO; MECHANICAL EFFECT ON CELL AND TISSUE; MECHANICAL EFFECTS; MECHANICAL LOADING; MECHANICAL STIMULUS; MESENCHYMAL STEM CELL; OSTEOGENIC DIFFERENTIATION; STEM CELL MODEL;

CELL CULTURE;

ALKALINE PHOSPHATASE; BONE MORPHOGENETIC PROTEIN 2;

ANIMAL CELL; ARTICLE; BONE DEVELOPMENT; CELL DIFFERENTIATION; ENZYME ACTIVITY; EX VIVO STUDY; GENETIC ENGINEERING; HUMAN; HUMAN CELL; MESENCHYMAL STEM CELL; NONHUMAN; OSTEOBLAST; PRIORITY JOURNAL; PROTEIN EXPRESSION;

EID: 79954582781     PISSN: 19326254     EISSN: 19327005     Source Type: Journal    
DOI: 10.1002/term.324     Document Type: Article

References (49)

1. Almany L, Seliktar D. 2005; Biosynthetic hydrogel scaffolds made from fibrinogen and polyethylene glycol for 3D cell cultures. Biomaterials 26(15): 2467-2477.
2. Altman GH, Horan RL, Martin I, et al. 2002; Cell differentiation by mechanical stress. FASEB J, 16(2): 270-272.
3. Angele P, Schumann D, Angele M, et al. 2004; Cyclic, mechanical compression enhances chondrogenesis of mesenchymal progenitor cells in tissue engineering scaffolds. Biorheology 41(3-4): 335-346.
4. Appelman TP, Mizrahi J, Elisseeff JH, et al. 2009; The differential effect of scaffold composition and architecture on chondrocyte response to mechanical stimulation. Biomaterials 30(4): 518-525.
5. Aslan H, Zilberman Y, Arbeli V, et al. 2006a; Nucleofection-based ex vivo nonviral gene delivery to human stem cells as a platform for tissue regeneration. Tissue Eng 12(4): 877-889.
6. + cells. Stem Cells 24(7): 1728-1737.
7. Chen HC, Lee HP, Sung ML, et al. 2004; A novel rotating-shaft bioreactor for two-phase cultivation of tissue-engineered cartilage. Biotechnol Prog 20(6): 1802-1809.
8. Chen HC, Sung LY, Lo WH, et al. 2008; Combination of baculovirus-expressed BMP-2 and rotating-shaft bioreactor culture synergistically enhances cartilage formation. Gene Ther 15(4): 309-317.
9. David V, Martin A, Lafage-Proust MH, et al. 2007; Mechanical loading downregulates peroxisome proliferator-activated receptor γ in bone marrow stromal cells and favors osteoblastogenesis at the expense of adipogenesis. Endocrinology 148(5): 2553-2562.
10. De Biase P, Capanna R. 2005; Clinical applications of BMPs. Injury 36(suppl 3): S43-46.
11. Dikovsky D, Bianco-Peled H, Seliktar D. 2006; The effect of structural alterations of PEG-fibrinogen hydrogel scaffolds on 3D cellular morphology and cellular migration. Biomaterials 27(8): 1496-1506.
12. Duda GN, Sporrer S, Sollmann M, et al. 2003; Interfragmentary movements in the early phase of healing in distraction and correction osteotomies stabilized with ring fixators. Langenbecks Arch Surg 387(11-12): 433-440.
13. Duty AO, Oest ME, Guldberg RE. 2007; Cyclic mechanical compression increases mineralization of cell-seeded polymer scaffolds in vivo. J Biomech Eng 129(4): 531-539.
14. Gafni Y, Pelled G, Zilberman Y, et al. 2004; Gene therapy platform for bone regeneration using an exogenously regulated, AAV-2-based gene expression system. Mol Ther 9(4): 587-595.
15. Gazit D, Turgeman G, Kelley P, et al. 1999; Engineered pluripotent mesenchymal cells integrate and differentiate in regenerating bone: a novel cell-mediated gene therapy. J Gene Med 1(2): 121-133.
16. Hagenmuller H, Hofmann S, Kohler T, et al. 2007; Non-invasive time-lapsed monitoring and quantification of engineered bone-like tissue. Ann Biomed Eng 35(10): 1657-1667.
17. Halstenberg S, Panitch A, Rizzi S, et al. 2002; Biologically engineered protein-graft-poly(ethylene glycol) hydrogels: a cell adhesive and plasmin-degradable biosynthetic material for tissue repair. Biomacromolecules 3(4): 710-723.
18. Hasharoni A, Zilberman Y, Turgeman G, et al. 2005; Murine spinal fusion induced by engineered mesenchymal stem cells that conditionally express bone morphogenetic protein-2. J Neurosurg Spine 3(1): 47-52.
19. Hildebrand T, Laib A, Muller R, et al. 1999; Direct three-dimensional morphometric analysis of human cancellous bone: microstructural data from spine, femur, iliac crest, and calcaneus. J Bone Miner Res 14(7): 1167-1174.
20. Huang CY, Hagar KL, Frost LE, et al. 2004; Effects of cyclic compressive loading on chondrogenesis of rabbit bone-marrow derived mesenchymal stem cells. Stem Cells 22(3): 313-323.
21. Jones DB, Nolte H, Scholubbers JG, et al. 1991; Biochemical signal transduction of mechanical strain in osteoblast-like cells. Biomaterials 12(2): 101-110.
22. Juncosa-Melvin N, Shearn JT, Boivin GP, et al. 2006; Effects of mechanical stimulation on the biomechanics and histology of stem cell-collagen sponge constructs for rabbit patellar tendon repair. Tissue Eng 12(8): 2291-2300.
23. Kang Q, Sun MH, Cheng H, et al. 2004; Characterization of the distinct orthotopic bone-forming activity of 14 BMPs using recombinant adenovirus-mediated gene delivery. Gene Ther 11(17): 1312-1320.
24. Kasper G, Dankert N, Tuischer J, et al. 2007; Mesenchymal stem cells regulate angiogenesis according to their mechanical environment. Stem Cells 25(4): 903-910.
25. Li JZ, Li H, Sasaki T, et al. 2003; Osteogenic potential of five different recombinant human bone morphogenetic protein adenoviral vectors in the rat. Gene Therapy 10: 1735-1743.
26. Liedert A, Kaspar D, Blakytny R, et al. 2006; Signal transduction pathways involved in mechanotransduction in bone cells. Biochem Biophys Res Commun 349(1): 1-5.
27. Matziolis G, Tuischer J, Kasper G, et al. 2006; Simulation of cell differentiation in fracture healing: mechanically loaded composite scaffolds in a novel bioreactor system. Tissue Eng 12(1): 201-208.
28. Moutsatsos IK, Turgeman G, Zhou S, et al. 2001; Exogenously regulated stem cell-mediated gene therapy for bone regeneration. Mol Ther 3(4): 449-461.
29. Muller R, Ruegsegger P. 1997; Micro-tomographic imaging for the nondestructive evaluation of trabecular bone architecture. Stud Health Technol Inform 40: 61-79.
30. Muller R, Van Campenhout H, Van Damme B, et al. 1998; Morphometric analysis of human bone biopsies: a quantitative structural comparison of histological sections and micro-computed tomography. Bone 23(1): 59-66.
31. Neidlinger-Wilke C, Wurtz K, Liedert A, et al. 2005; A three-dimensional collagen matrix as a suitable culture system for the comparison of cyclic strain and hydrostatic pressure effects on intervertebral disc cells. J Neurosurg Spine 2(4): 457-465.
32. Neven E, Dams G, Postnov A, et al. 2009; Adequate phosphate binding with lanthanum carbonate attenuates arterial calcification in chronic renal failure rats. Nephrol Dial Transpl 24(6): 1790-1799.
33. Noel D, Gazit D, Bouquet C, et al. 2004; Short-term BMP-2 expression is sufficient for in vivo osteochondral differentiation of mesenchymal stem cells. Stem Cells 22(1): 74-85.
34. Peled E, Boss J, Bejar J, et al. 2007; A novel poly(ethylene glycol)-fibrinogen hydrogel for tibial segmental defect repair in a rat model. J Biomed Mater Res A 80(4): 874-884.
35. Pelled G, Tai K, Sheyn D, et al. 2007; Structural and nanoindentation studies of stem cell-based tissue-engineered bone. J Biomech 40(2): 399-411.
36. Pittenger MF, Mosca JD, McIntosh KR. 2000; Human mesenchymal stem cells: progenitor cells for cartilage, bone, fat and stroma. Curr Top Microbiol Immunol 251: 3-11.
37. Preiss-Bloom O, Mizrahi J, Elisseeff J, et al. 2009; Real-time monitoring of force response measured in mechanically stimulated tissue-engineered cartilage. Artif Organs 33(4): 318-327.
38. Rawadi G, Vayssiere B, Dunn F, et al. 2003; BMP-2 controls alkaline phosphatase expression and osteoblast mineralization by a Wnt autocrine loop. J Bone Miner Res 18(10): 1842-1853.
39. Robling AG, Castillo AB, Turner CH. 2006; Biomechanical and molecular regulation of bone remodeling. Annu Rev Biomed Eng 8: 455-498.
40. Schmidt O, Mizrahi J, Elisseeff J, et al. 2006; Immobilized fibrinogen in PEG hydrogels does not improve chondrocyte-mediated matrix deposition in response to mechanical stimulation. Biotechnol Bioeng 95(6): 1061-1069.
41. Sheyn D, Kimelman-Bleich N, Pelled G, et al. 2008; Ultrasound-based nonviral gene delivery induces bone formation in vivo. Gene Ther 15(4): 257-266.
42. Siddhivarn C, Banes A, Champagne C, et al. 2007; Mechanical loading and δ-12 prostaglandin J2 induce bone morphogenetic protein-2, peroxisome proliferator-activated receptor γ-1, and bone nodule formation in an osteoblastic cell line. J Periodontal Res 42(5): 383-392.
43. Terraciano V, Hwang N, Moroni L, et al. 2007; Differential response of adult and embryonic mesenchymal progenitor cells to mechanical compression in hydrogels. Stem Cells 25(11): 2730-2738.
44. Turner CH, Owan I, Alvey T, et al. 1998; Recruitment and proliferative responses of osteoblasts after mechanical loading in vivo determined using sustained-release bromodeoxyuridine. Bone 22(5): 463-469.
45. Wang N, Butler JP, Ingber DE. 1993; Mechanotransduction across the cell surface and through the cytoskeleton. Science 260(5111): 1124-1127.
46. Xie C, Reynolds D, Awad H, et al. 2007; Structural bone allograft combined with genetically engineered mesenchymal stem cells as a novel platform for bone tissue engineering. Tissue Eng 13(3): 435-445.
47. Yamaguchi A. 1995; Regulation of differentiation pathway of skeletal mesenchymal cells in cell lines by transforming growth factor-β superfamily. Semin Cell Biol 6(3): 165-173.
48. Yamaguchi A, Komori T, Suda T. 2000; Regulation of osteoblast differentiation mediated by bone morphogenetic proteins, hedgehogs, and Cbfa1. Endocr Rev 21(4): 393-411.
49. Young RG, Butler DL, Weber W, et al. 1998; Use of mesenchymal stem cells in a collagen matrix for Achilles tendon repair. J Orthop Res 16(4): 406-413.