Aneuploidy in human spermatozoa

Cytogenetic and Genome Research

Volumn 133, Issue 2-4, 2011, Pages 91-99

  Templado, C ^a,b   Vidal, F ^b   Estop, A ^c  

^a Facultat de Medicina   (Spain)

^b UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

^c Reprogenetics LLC   (United States)

Author keywords

Aneuploidy; FISH; Spermatozoa

Indexed keywords

ALCOHOL; CAFFEINE;

AGE; ALCOHOL CONSUMPTION; ANEUPLOIDY; ARTICLE; AUTOSOME; CELL NUCLEUS; CHROMOSOME 21; CHROMOSOME 22; CHROMOSOME 8; DIPLOIDY; DISOMY; DOWN SYNDROME; FATHER; FLUORESCENCE IN SITU HYBRIDIZATION; HUMAN; KARYOTYPE; KARYOTYPE 47,XXY; KARYOTYPE 47,XYY; KLINEFELTER SYNDROME; PRIORITY JOURNAL; PROGENY; SEX CHROMOSOME; SEX CHROMOSOME ABERRATION; SMOKING; SPERMATOZOON; TURNER SYNDROME;

AGE FACTORS; ANEUPLOIDY; ANIMALS; CELL NUCLEUS; CHROMOSOMES, HUMAN; HUMANS; LIFE STYLE; MALE; SPERMATOZOA;

EID: 79954571969     PISSN: 14248581     EISSN: None     Source Type: Journal    
DOI: 10.1159/000323795     Document Type: Article

References (99)

1. Angell RR: Predivision in human oocytes at meiosis I: a mechanism for trisomy formation in man. Hum Genet 86: 383-387 (1991).
2. Arnedo N, Templado C, Sánchez-Blanque Y, Rajmil O, Nogués C: Sperm aneuploidy in fathers of Klinefelter's syndrome offspring assessed by multicolour fluorescent in situ hybridization using probes for chromosomes 6, 13, 18, 21, 22, X and Y. Hum Reprod 21: 524-528 (2006). (Pubitemid 43159932)
3. Baumgartner A, Schmid TE, Maerz HK, Adler ID, Tarnok A, Nuesse M: Automated evaluation of frequencies of aneuploid sperm by laser-scanning cytometry (LSC). Cytometry 44: 156-160 (2001).
4. Bergère M, Wainer R, Nataf V, Bailly M, Gombault M, Ville Y, Selva J: Biopsied testis cells of four 47,XXY patients: fluorescence in-situ hybridization and ICSI results. Hum Reprod 17: 32-37 (2002). (Pubitemid 34130257)
5. Blanco J, Rubio C, Simón C, Egozcue J, Vidal F: Increased incidence of disomic sperm nuclei in a 47,XYY male assessed by fluorescent in situ hybridization (FISH). Hum Genet 99: 413-416 (1997). (Pubitemid 27095807)
6. Blanco J, Gabau E, Gómez D, Baena N, Guitart M, Egozcue J, Vidal F: Chromosome 21 disomy in the spermatozoa of the fathers of children with trisomy 21, in a population with a high prevalence of Down syndrome: increased incidence in cases of paternal origin. Am J Hum Genet 63: 1067-1072 (1998). (Pubitemid 30418576)
7. Blanco J, Egozcue J, Vidal F: Meiotic behaviour of the sex chromosomes in three patients with sex chromosome anomalies (47,XXY, mosaic 46,XY/47,XXY and 47,XYY) assessed by fluorescence in-situ hybridization. Hum Reprod 16: 887-892 (2001). (Pubitemid 32451823)
8. Bosch M, Rajmil O, Martínez-Pasarell O, Egozcue J, Templado C: Linear increase of diploidy in human sperm with age: a fourcolour FISH study. Eur J Hum Genet 9: 533-538 (2001). (Pubitemid 32750494)
9. Bosch M, Rajmil O, Egozcue J, Templado C: Linear increase of structural and numerical chromosome 9 abnormalities in human sperm regarding age. Eur J Hum Genet 11: 754-759 (2003). (Pubitemid 37337058)
10. Brandriff B, Gordon L: Human sperm cytogenetics and the one-cell zygote, in Allen JW, Bridges BA, Lyon MF, Moses MJ, Russell RB (eds): Biology of Mammalian Germ Cell Mutagenesis, Banbury Report 34, pp 183-194 (Cold Spring Harbor Laboratory Press, Cold Spring Harbor 1990).
11. Burgoyne PS, Mahadevaiah SK, Turner JM: The consequences of asynapsis for mammalian meiosis. Nat Rev Genet 10: 207-216 (2009).
12. Buwe A, Guttenbach M, Schmid M: Effect of paternal age on the frequency of cytogenetic abnormalities in human spermatozoa. Cytogenet Genome Res 111: 213-228 (2005). (Pubitemid 41414471)
13. Carrell D, Emery B: Use of automated imaging and analysis technology for the detection of aneuploidy in human sperm. Fertil Steril 90: 434-437 (2008). (Pubitemid 352040714)
14. De Mas P, Daudin M, Vincent MC, Bourrouillou G, Calvas P, et al: Increased aneuploidy in spermatozoa from testicular tumour patients after chemotherapy with cisplatin, etoposide and bleomycin. Hum Reprod 16: 1204-1208 (2001). (Pubitemid 32544692)
15. De Souza E, Halliday J, Chan A, Bower C, Morris JK: Recurrence risks for trisomies 13, 18, and 21. Am J Med Genet A 149A:2716-2722 (2009).
16. Downie SE, Flaherty SP, Swann NJ, Matthews CD: Estimation of aneuploidy for chromosomes 3, 7, 16, X and Y in spermatozoa from 10 normospermic men using fluorescence in-situ hybridization. Mol Hum Reprod 3: 815-819 (1997). (Pubitemid 127509617)
17. Egozcue J, Blanco J, Vidal F: Chromosome studies in human sperm nuclei using fluorescence in-situ hybridization (FISH). Hum Reprod Update 3: 441-452 (1997). (Pubitemid 28069950)
18. Egozcue S, Blanco J, Vendrell JM, García F, Veiga A, et al: Human male infertility: chromosome anomalies, meiotic disorders, abnormal spermatozoa and recurrent abortion. Hum Reprod Update 6: 93-105 (2000). (Pubitemid 30099347)
19. Eskenazi B, Wyrobek AJ, Kidd SA, Lowe X, Moore D 2nd, et al: Sperm aneuploidy in fathers of children with paternally and maternally inherited Klinefelter syndrome. Hum Reprod 17: 576-583 (2002). (Pubitemid 34232423)
20. Estop AM, Cieply K, Munné S, Surti U, Wakim A, Feingold E: Is there an interchromosomal effect in reciprocal translocation carriers? Sperm FISH studies. Hum Genet 106: 517-524 (2000). (Pubitemid 30406677)
21. Ferlin A, Garolla A, Foresta C: Chromosome abnormalities in sperm of individuals with constitutional sex chromosome abnormalities. Cytogenet Genome Res 111: 310-316 (2005). (Pubitemid 41414484)
22. Finch KA, Fonseka KGL, Abrogrein A, Ioannou D, Handyside AH, et al: Nuclear organization in human sperm: preliminary evidence for altered sex chromosome centromere position in infertile males. Hum Reprod 23: 1263-1270 (2008). (Pubitemid 351770588)
23. Foresta C, Galeazzi C, Bettella A, Marin P, Rossato M, et al: Analysis of meiosis in intratesticular germ cells from subjects affected by classic Klinefelter's syndrome. J Clin Endocrinol Metab 84: 3807-3810 (1999). (Pubitemid 30645245)
24. Garcia-Cruz R, Casanovas A, Brieño-Enríquez M, Robles P, Roig I, et al: Cytogenetic analyses of human oocytes provide new data on non-disjunction mechanisms and the origin of trisomy 16. Hum Reprod 25: 179-191 (2010).
25. Gardner RJ, Sutherland GR: Chromosome Abnormalities and Genetic Counseling, 3rd ed. (Oxford University Press, New York 2004).
26. Griffin DK, Abruzzo MA, Millie EA, Sheean LA, Feingold E, et al: Non-disjunction in human sperm: evidence for an effect of increasing paternal age. Hum Mol Genet 12: 2227-2232 (1995).
27. Handel MA, Schimenti JC: Genetics of mammalian meiosis: regulation, dynamics and impact on fertility. Nat Rev Genet 11: 124-136 (2010).
28. Härkönen K, Viitanen T, Larsen S, Bonde J, Lähdetie J: Aneuploidy in sperm and exposure to fungicides and lifestyle factors. ASCLEPIOS.
29. Härkönen K, Suominen J, Lähdetie J: Aneuploidy in spermatozoa of infertile men with teratozoospermia. Int J Androl 24: 197-205 (2001). (Pubitemid 32675086)
30. Härkönen K: Pesticides and the induction of aneuploidy in human sperm. Cytogenet Genome Res 111: 378-383 (2005). (Pubitemid 41414494)
31. Hassold T, Hunt P: To err (meiotically) is human: the genesis of human aneuploidy. Nat Rev Genet 2: 280-291 (2001). (Pubitemid 33674776)
32. Hassold T, Abruzzo M, Adkins K, Griffin D, Merrill M, et al: Human aneuploidy: incidence, origin, and etiology. Environ Mol Mutagen 28: 167-175 (1996). (Pubitemid 26380273)
33. Hassold T, Hall H, Hunt P: The origin of human aneuploidy: where we have been, where we are going. Hum Mol Genet 16: 203-208 (2007).
34. Hennebicq S, Pelletier R, Bergues U, Rousseaux S: Risk of trisomy 21 in offspring of patients with Klinefelter's syndrome. Lancet 357: 2104-2105 (2001). (Pubitemid 32607458)
35. Hixon M, Millie E, Judis LA, Sherman S, Allran K, et al: FISH studies of the sperm of fathers of paternally derived cases of trisomy 21: no evidence for an increase in aneuploidy. Hum Genet 103: 654-657 (1998). (Pubitemid 29020759)
36. Jones KT: Meiosis in oocytes: predisposition to aneuploidy and its increased incidence with age. Hum Reprod Update 14: 143-158 (2008). (Pubitemid 351321109)
37. Kinakin B, Rademaker A, Martin R: Paternal age effect of YY aneuploidy in human sperm, as assessed by fluorescence in situ hybridization. Cytogenet Cell Genet 78: 116-119 (1997). (Pubitemid 27480761)
38. Kirkpatrick G, Ferguson KA, Gao H, Tang S, Chow V, et al: A comparison of sperm aneuploidy rates between infertile men with normal and abnormal karyotypes. Hum Reprod 23: 1679-1683 (2008). (Pubitemid 351809710)
39. Lähdetie J, Saari N, Ajosenpaa-Saari M, Mykkanen J: Incidence of aneuploid spermatozoa among infertile men studied by multicolor fluorescence in situ hybridization. Am J Med Genet 71: 115-121 (1997).
40. Lanfranco F, Kamischke A, Zitzmann M, Nieschlag E: Klinefelter's syndrome. Lancet 364: 273-283 (2004). (Pubitemid 38942818)
41. Márquez C, Egozcue J, Martorell MR, Moreno V, Templado C: Colcemid increases the frequency of chromosome abnormalities in human sperm. Cytogenet Cell Genet 72: 164-170 (1996). (Pubitemid 26079345)
42. Martin RH, Rademaker AW: The frequency of aneuploidy among individual chromosomes in 6,821 human sperm chromosome complements. Cytogenet Cell Genet 53: 103-107 (1990). (Pubitemid 20189227)
43. Martin RH, Rademaker AW: Nondisjunction in human sperm: comparison of frequencies in acrocentric chromosomes. Cytogenet Cell Genet 86: 43-45 (1999). (Pubitemid 29417830)
44. Martin RH, Spriggs E, Ko E, Rademaker AW: The relationship between paternal age, sex ratios, and aneuploidy frequencies in human sperm, as assessed by multicolor FISH. Am J Hum Genet 57: 1395-1399 (1995).
45. Martínez-Pasarell O, Nogués C, Bosch M, Egozcue J, Templado C: Analysis of sex chromosome aneuploidy in sperm from fathers of Turner syndrome patients. Hum Genet 104: 345-349 (1999). (Pubitemid 29251154)
46. Mau-Holzmann UA: Somatic chromosomal abnormalities in infertile men and women. Cytogenet Genome Res 111: 317-336 (2005). (Pubitemid 41414485)
47. McInnes B, Rademaker A, Martin RH: Donor age and the frequency of disomy for chromosomes 1, 13, 21 and structural abnormalities in human spermatozoa using multicolour fluorescence in-situ hybridization. Hum Reprod 13: 2489-2494 (1998). (Pubitemid 28451229)
48. Milazzo JP, Rives N, Mousset-Siméon N, Macé B: Chromosome constitution and apoptosis of immature germ cells present in sperm of two 47,XYY infertile males. Hum Reprod 21: 1749-1758 (2006). (Pubitemid 44028245)
49. Molina O, Sarrate Z, Vidal F, Blanco J: FISH on sperm: spot counting to stop counting? Not yet. Fertil Steril 92: 1474-1480 (2009).
50. Moosani N, Chernos J, Lowry RB, Martin RH, Rademaker A: A 47,XXY fetus resulting from ICSI in a man with an elevated frequency of 24,XY spermatozoa. Hum Reprod14: 1137-1138 (1999). (Pubitemid 29176502)
51. Mroz K, Hassold TJ, Hunt P: Meiotic aneuploidy in the XXY mouse, evidence that a compromised testicular environment increases the incidence of meiotic errors. Hum Reprod 14: 1151-1156 (1999). (Pubitemid 29224881)
52. Munné S, Sandalinas M, Magli C, Gianaroli L, Cohen L, et al: Increased rate of aneuploid embryos in young women with previous aneuploid conceptions. Prenat Diagn 24: 638-643 (2004). (Pubitemid 39108572)
53. Naccarati A, Zanello A, Landi S, Consigli R, Migliore L: Sperm-FISH analysis and human monitoring: a study on workers occupationally exposed to styrene. Mutat Res 537: 131-140 (2003). (Pubitemid 36638635)
54. Nishikawa N, Murakami I, Ikuta K, Suzumori K: Sex chromosomal analysis of spermatozoa from infertile men using fluorescence in situ hybridization. J Assist Reprod Genet 17: 97-102 (2000). (Pubitemid 30225291)
55. Ong TD, Xun L, Perreault SD, Robbins W: Aneuploidy and chromosome breakage in swim-up versus unprocessed semen from twenty healthy men. J Androl 23: 270-276 (2002).
56. Padungtod C, Hassold TJ, Millie E, Ryan LM, Savitz DA, et al: Sperm aneuploidy among Chinese pesticide factory workers: scoring by the FISH method. Am J Ind Med 36: 230-238 (1999). (Pubitemid 29328186)
57. Pellestor F, Girardet A, Coignet L, Andréo B, Charlieu JP: Assessment of aneuploidy for chromosomes 8, 9, 13, 16, and 21 in human sperm by using primed in situ labelling technique. Am J Hum Genet 58: 797-802 (1996). (Pubitemid 26086671)
58. Pellestor F, Andréo B, Anahory T, Hamamah S: The occurrence of aneuploidy in human: lessons from the cytogenetic studies of human oocytes. Eur J Med Genet 49: 103-116 (2006). (Pubitemid 43374815)
59. Rives N, Joly G, Machy A, Simeon N, Leclerc P, Mace B: Assessment of sex chromosome aneuploidy in sperm nuclei from 47,XXY and 46,XY/47,XXY males: comparison with fertile and infertile males with normal karyotype. Mol Hum Reprod 6: 107-112 (2000). (Pubitemid 30094273)
60. Robbins WA, Baulch JE, Moore D 2nd, Weier HU, Blakey D, Wyrobek AJ: Three-probe fluorescence in situ hybridization to assess chromosome X, Y, and 8 aneuploidy in sperm of 14 men from two healthy groups: evidence for a paternal age effect on sperm aneuploidy. Reprod Fertil Dev 7: 799-809 (1995).
61. Robbins WA, Vine MF, Truong KY, Everson RB: Use of FISH (Fluorescence in situ hybridization) to assess effects of smoking, caffeine, and alcohol on aneuploidy load in sperm of healthy men. Environ Mol Mutagen 30: 175-183 (1997). (Pubitemid 27440594)
62. Robbins WA, Elashoff DA, Xun L, Jia J, Li N, et al: Effect of lifestyle exposures on sperm aneuploidy. Cytogenet Genome Res 111: 371-377 (2005). (Pubitemid 41414493)
63. Rodrigo L, Rubio C, Mateu E, Simon C, Remohi J, et al: Analysis of chromosomal abnormalities in testicular and epididymal spermatozoa from azoospermic ICSI patients by fluorescence in-situ hybridization. Hum Reprod 19: 118-123 (2004). (Pubitemid 38072019)
64. Rodrigo L, Peinado V, Mateu E, Remohí J, Pellicer A, et al: Impact of different patterns of sperm chromosomal abnormalities on the chromosomal constitution of preimplantation embryos. Fertil Steril 94: 1380-1386 (2010).
65. Rousseaux S, Hazzouri M, Pelletier R, Monteil M, Usson Y, Sele B: Disomy rates for chromosomes 14 and 21 studied by fluorescent insitu hybridization in spermatozoa from three men over 60 years of age. Mol Hum Reprod 4: 695-699 (1998).
66. Rubes J, Lowe X, Moore D, Perreault S, Slott V, et al: Smoking cigarettes is associated with increased sperm disomy in teenage men. Fertil Steril 70: 715-724 (1998).
67. Rubes J, Vozdova M, Robbins WA, Rezacova O, Perreault SD, Wyrobek AJ: Stable variants of sperm aneuploidy among healthy men show associations between germinal and somatic aneuploidy. Am J Hum Genet 70: 1507-1519 (2002). (Pubitemid 34533898)
68. Rubes J, Vozdova M, Oracova E, Perreault SD: Individual variations in the frequency of sperm aneuploidy in humans. Cytogenet Genome Res 111: 229-236 (2005). (Pubitemid 41414472)
69. Rubio C, Simón C, Blanco J, Vidal F, Mínguez Y, et al: Implications of sperm chromosome abnormalities in recurrent miscarriage. J Assist Reprod Genet 16: 253-258 (1999). (Pubitemid 29226909)
70. Sarrate Z, Blanco J, Anton E, Egozcue S, Egozcue J, Vidal F: FISH studies of chromosome abnormalities in germ cells and its relevance in reproductive counselling. Asian J Androl 7: 227-236 (2005). (Pubitemid 41288544)
71. Sarrate Z, Blanco J, Anton E, Egozcue S, Egozcue J, Vidal F: Role of sperm fluorescent in situ hybridization studies in infertile patients: indications, study approach, and clinical relevance. Fertil Steril 93: 1892-1902 (2010).
72. Scarpato R, Naccarati A, Mariani M, Migliore L: Aneuploidy and diploidy rates in sperm of five men after three-colour hybridization: indication of X chromosome-associated autosome 2 aneuploidy. Mutat Res 412: 227-233 (1998). (Pubitemid 28218252)
73. Sciurano RB, Luna Hisano CV, Rahn MI, Brugo Olmedo S, Rey Valzacchi G, et al: Focal spermatogenesis originates in euploid germ cells in classical Klinefelter patients. Hum Reprod 24: 2353-2361 (2009).
74. Shi Q, Martin RH: Spontaneous frequencies of aneuploid and diploid sperm in 10 normal Chinese men: assessed by multicolour fluorescence in situ hybridization. Cytogenet Cell Genet 90: 79-83 (2000).
75. Shi Q, Martin RH: Aneuploidy in human spermatozoa: FISH analysis in men with constitutional chromosomal abnormalities, and in infertile men. Reproduction 121: 655-666 (2001). (Pubitemid 32472051)
76. Shi Q, Ko E, Barclay L, Hoang T, Rademaker A, Martin R: Cigarette smoking and aneuploidy in human sperm. Mol Reprod Dev 59: 417-421 (2001). (Pubitemid 32601847)
77. Skakkebaek N, Philip J, Hammen R: Meiotic chromosomes in Klinefelter's syndrome. Nature 221: 1075-1076 (1969).
78. Sloter E, Nath J, Eskenazi B, Wyrobek AJ: Effects of male age on the frequencies of germinal and heritable chromosomal abnormalities in humans and rodents. Fertil Steril 81: 925-943 (2004). (Pubitemid 38452952)
79. Smith JL, Garry VF, Rademaker AW, Martin RH: Human sperm aneuploidy after exposure to pesticides. Mol Reprod Dev 67: 353-359 (2004). (Pubitemid 38140831)
80. Soares SR, Templado C, Blanco J, Egozcue J, Vidal F: Numerical chromosome abnormalities in the spermatozoa of the fathers of children with trisomy 21 of paternal origin: generalised tendency to meiotic non-disjunction. Hum Genet 108: 134-139 (2001a). (Pubitemid 32215373)
81. Soares SR, Vidal F, Bosch M, Martínez-Pasarell O, Nogués C, et al: Acrocentric chromosome disomy is increased in spermatozoa from fathers of Turner syndrome patients. Hum Genet 108: 499-503 (2001b). (Pubitemid 32700352)
82. Spriggs EL, Rademaker AW, Martin RH: Aneuploidy in human sperm: results of two-and three-color fluorescence in situ hybridization using centromeric probes for chromosomes 1, 12, 15, 18, X, and Y. Cytogenet Cell Genet 71: 47-53 (1995).
83. Spriggs EL, Rademaker AW, Martin RH: Aneuploidy in human sperm: the use of multicolor FISH to test various theories of nondisjunction. Am J Hum Genet 58: 356-362 (1996). (Pubitemid 26038941)
84. Staessen C, Tournaye H, Van Assche E, Michiels A, Van Landuyt L, et al: PGD in 47,XXY Klinefelter's syndrome patients. Hum Reprod Update 9: 319-330 (2003). (Pubitemid 36987281)
85. Sun F, Mikhaail-Philips M, Oliver-Bonet M, Ko E, Rademaker A, et al: The relationship between meiotic recombination in human spermatocytes and aneuploidy in sperm. Hum Reprod 23: 1691-1697 (2008). (Pubitemid 352019995)
86. Tang SS, Gao H, Robinson WP, Ho Yuen B, Ma S: An association between sex chromosomal aneuploidy in sperm and an abortus with 45,X of paternal origin: possible transmission of chromosomal abnormalities through ICSI. Hum Reprod 19: 147-151 (2004). (Pubitemid 38072023)
87. Tang SS, Gao H, Zhao Y, Ma S: Aneuploidy and DNA fragmentation in morphologically abnormal sperm. Int J Androl 33: e163-e179 (2010).
88. Tempest HG, Ko E, Rademaker A, Chan P, Robaire B, Martin RH: Intra-individual and inter-individual variations in sperm aneuploidy frequencies in normal men. Fertil Steril 91: 185-191 (2009).
89. Tempest HG, Cheng SY, Gillott DJ, Handyside AH, Thornhill AR, Griffin DK: Scoring of sperm chromosomal abnormalities by manual and automated approaches: qualitative and quantitative comparisons. Asian J Androl 12: 257-262 (2010).
90. Templado C, Márquez C, Munné S, Colls P, Martorell MR, et al: An analysis of human sperm chromosome aneuploidy. Cytogenet Cell Genet 74: 194-200 (1996). (Pubitemid 26383964)
91. Templado C, Bosch M, Benet J: Frequency and distribution of chromosome abnormalities in human spermatozoa. Cytogenet Genome Res 111: 119-205 (2005).
92. Uroz L, Rajmil O, Templado C: Premature separation of sister chromatids in human male meiosis. Hum Reprod 23: 982-987 (2008).
93. Uroz L, Rajmil O, Templado C: Meiotic chromosome abnormalities in fertile men: are they increasing? Fertil Steril 95;141-146 (2011).
94. Vanneste E, Voet T, Le Caignec C, Ampe M, Konings P, et al: Chromosome instability is common in human cleavage-stage embryos. Nat Med 15: 577-583 (2009).
95. Vialard F, Pellestor F: Intérêt de la cytogéné tique des gamètes humains: résultats et perspectives. Pathologie Biologie 56: 388-399 (2008).
96. Warburton D, Dallaire L, Thangavelu M, Ross L, Levin B, Kline J: Trisomy Recurrence: A Reconsideration Based on North American Data. Am J Hum Genet 75: 376-385 (2004). (Pubitemid 39095814)
97. Wong EC, Ferguson KA, Chow V, Ma S: Sperm aneuploidy and meiotic sex chromosome configurations in an infertile XYY male. Hum Reprod 23: 374-378 (2008). (Pubitemid 351651254)
98. Wyrobek AJ, Eskenazi B, Young S, Arnheim N, Tiemann-Boege I, et al: Advancing age has differential effects on DNA damage, chromatin integrity, gene mutations, and aneuploidies in sperm. Proc Natl Acad Sci USA 103: 9601-9606 (2006). (Pubitemid 43955871)
99. Xia Y, Bian Q, Xu L, Cheng S, Song L, et al: Genotoxic effects on human spermatozoa among pesticide factory workers exposed to fenvalerate. Toxicology 203: 49-60 (2004). (Pubitemid 39200937)