Mechanistic and structural insights into the regioselectivity of an Acyl-CoA fatty acid desaturase via directed molecular evolution

Journal of Biological Chemistry

Volumn 286, Issue 15, 2011, Pages 12860-12869

  Vanhercke, Thomas ^a   Shrestha, Pushkar ^a   Green, Allan G ^a   Singh, Surinder P ^a  

^a CSIRO   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVE SITE; ACYL CARRIER; ACYL-COA; BIFUNCTIONAL; BIOCHEMICAL DATA; CATALYTIC DIVERSITY; COMPLEMENTATION; DESATURASE; DESATURATION; DIRECTED EVOLUTION; DOUBLE BONDS; IN-VITRO; MEMBRANE-BOUND; MOLECULAR EVOLUTION; SHORTER CHAINS; STRUCTURAL BASIS; STRUCTURAL INSIGHTS; SUBSTRATE SPECIFICITY;

BIOCHEMISTRY; CATALYSTS; CRYSTAL STRUCTURE; ENZYMES; MOLECULAR BIOLOGY; REGIOSELECTIVITY; SUBSTRATES;

UNSATURATED FATTY ACIDS;

ACYL COENZYME A DESATURASE; FATTY ACID ESTER;

AMINO ACID SUBSTITUTION; ARTICLE; CRICKET; DIRECTED MOLECULAR EVOLUTION; ENZYME ACTIVITY; ENZYME SPECIFICITY; ENZYME SUBSTRATE; FATTY ACID ANALYSIS; FATTY ACID DESATURATION; FATTY ACID SYNTHESIS; INSECT; LIPID ANALYSIS; MUTAGENESIS; NONHUMAN; PHYLOGENY; PRIORITY JOURNAL;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMALS; CATALYTIC DOMAIN; DIRECTED MOLECULAR EVOLUTION; FATTY ACID DESATURASES; FATTY ACIDS; GRYLLIDAE; INSECT PROTEINS; MOLECULAR SEQUENCE DATA; MUTATION, MISSENSE; SACCHAROMYCES CEREVISIAE; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY;

ACHETA DOMESTICUS;

EID: 79953864232     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M110.191098     Document Type: Article

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