Mutation study of conserved amino acid residues of Spirulina Δ6-acyl-lipid desaturase showing involvement of histidine 313 in the regioselectivity of the enzyme

Applied Microbiology and Biotechnology

Volumn 66, Issue 1, 2004, Pages 74-84

  Hongsthong, Apiradee ^a   Subudhi, Sanjukta ^a   Sirijuntarat, Matura ^a   Cheevadhanarak, Supapon ^a  

^a KING MONGKUT'S UNIVERSITY OF TECHNOLOGY THONBURI   (Thailand)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYSIS; ENZYMES; ESCHERICHIA COLI; ORGANIC ACIDS; SUBSTRATES;

CYANOBACTERIAL SPECIES; MUTATION; REGIOSELECTIVITY; SUBSTRATE BINDING;

AMINO ACIDS;

BACTERIAL ENZYME; HISTIDINE; LINOLEIC ACID;

AMINO ACID; ENZYME ACTIVITY;

ARTICLE; CATALYSIS; CYANOBACTERIUM; ENZYME ACTIVITY; ENZYME SUBSTRATE COMPLEX; ESCHERICHIA COLI; GENE EXPRESSION; GENE EXPRESSION SYSTEM; GENETIC CONSERVATION; NONHUMAN; PROTEIN DETERMINATION; PROTEIN FUNCTION; SEQUENCE ALIGNMENT; SITE DIRECTED MUTAGENESIS; SPIRULINA;

AMINO ACID SEQUENCE; BINDING SITES; CONSERVED SEQUENCE; CYANOBACTERIA; FATTY ACID DESATURASES; HISTIDINE; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; SUBSTRATE SPECIFICITY;

CYANOBACTERIA; ESCHERICHIA COLI; SPIRULINA; SPIRULINA PLATENSIS;

EID: 12444272688     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-004-1655-x     Document Type: Article

References (35)

1. Avelange-Macherel M, Macherel D, Wada H, Murata N (1991) Site-directed mutagenesis of histidine residues in the delta 12 acyl-lipid desaturase of Synechocystis. FEBS 361:111-114
2. Bloomfield DK, Bloch K (1960) Formation of unsaturated fatty acids. J Biol Chem 235:337-345
3. Broadwater JA, Whittle E, Shanklin J (2002) Desaturation and hydroxylation; residue 148 and 324 of Arabidopsis FAD2, in addition to substrate chain length, exert a major influence in partitioning of catalytic specificity. Biol Chem 277:15613-15620
4. Cahoon EB, Mills LA, Shanklin J (1996) Modification of the fatty acid composition of Escherichia coli by coexpression of a plant acyl-acyl carrier protein desaturase and ferredoxin. J Bacteriol 178:936-939
5. Cahoon EB, Coughlan SJ, Shanklin J (1997) Characterization of a structurally and functionally diverged acyl-acyl carrier protein desaturase from milkweed seed. Plant Mol Biol 33:1105-1110
6. Cahoon EB, Lindqvist Y, Schneider G, Shanklin J (1997) Redesign of soluble fatty acid desaturases from plants for altered substrate specificity and double bond position. Proc Natl Acad Sci USA 94:4872-4877
7. Cahoon EB, Shah S, Shanklin J, Browse J (1998) A determinant of substrate specificity predicted from the acyl-acyl carrier protein desaturase of developing cat's claw seed. Plant Physiol 117:593-598
8. Creighton TE (1993) Proteins: structures and molecular properties. Freeman, New York
9. Deng WP, Nickoloff JA (1992) Site directed mutagenesis of virtually any plasmid by eliminating a unique site. Anal Biochem 200:81-88
10. Deshnium P, Paithoonrangsarid K, Suphratrakul A, Meesapyodsuk D, Tanticharoen M, Cheevadhanarak S (2000) Temperature-independent and dependent expression of desaturase genes in filamentous cyanobacterium Spirulina platensis C1 (Arthrospira sp. PCC 9438). FEMS Microbiol Lett 184:207-213
11. Diaz AR, Mansilla MC, Vila AJ, Mendoza D (2002) Membrane topology of the acyl-lipid desaturase from Bacillus subtilis. Biol Chem 277:48099-48106
12. Esfahani M, Barnes EMJ, Wakil S (1969) Control of fatty acid composition in phospholipids of Escherichia coli: response to fatty acid supplements in a fatty acid auxotroph. Proc Natl Acad Sci USA 64:1057-1064
13. Fay L, Richli U (1991) Location of double bonds in polyunsaturated fatty acids by gas chromatography-mass spectrometry after 4,4-dimethyloxazoline derivatization. J Chromatogr 541:89-98
14. Gravel P, Golaz O (1996) Protein blotting by the semi-dry method. In: Walker JM (ed) The protein protocols handbook. Humana, New Jersey, pp 249-260
15. Holloway PW (1983) Fatty acid desaturation. In Boyer PD (ed) The enzymes, vol 16. Academic, Orlando, pp 63-83
16. Hongsthong A, Deshnium P, Paithoonrangsarid K, Cheevadhanarak S, Tanticharoen M (2003) Differential responses of the three acyl-lipid desaturases to immediate temperature reduction occurred in two lipid membranes of Spirulina platensis strain C1. J Biosci Bioeng 96:519-524
17. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:269-275
18. Mcguire KA, Siggaard-Andersen M, Bangera MG, Olsen JG, Wettstein-Knowles P (2001) β-Ketoacyl-(acyl carrier protein) synthase I of Escherichia coli: aspects of the condensation mechanism revealed by analyses of mutations in the active site pocket. Biochemistry 40:9836-9845
19. Murata N, Nishida I (1987) Lipids of blue-green algae. In: Stumpf PK (ed) The biochemistry of plants, vol 9. Academic, Orlando, pp 315-347
20. Murata N, Wada H (1995) Acyl-lipid desaturases and their importance in the tolerance and acclimation to cold of cyanobacteria. Biochem J 308:1-8
21. Murata N, Deshnium P, Tasaka Y (1996) Biosynthesis of γ-linolenic acid in the cyanobacterium Spirulina platensis. In: Huang YS, Mills DE (eds) γ-Linolenic acid metabolism and its roles in nutrition and medicine. AOCS, Urbana-Champaign, pp 22-32
22. Mustady L, Los DA, Gombos Z, Murata N (1996) Immunocytochemical location of acyl-lipid desaturases in cyanobacterial cells: evidence that both thylakoid membranes and cytoplasmic membranes are the sites for lipid desaturation. Proc Natl Acad Sci USA 93:10524-10527
23. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
24. Schmidt H, Heinz E (1993) Direct desaturation of intact galactolipids by a desaturase solubilized from spinach (Spinacia oleracea) chloroplast envelopes. Biochem J 289:777-782
25. Schmidt H, Sperling P, Heinz E (1993) Biochemistry and molecular biology of membrane and storage lipids of plants. American Society for Plant Physiologists, Rockville
26. Schultz DJ, Chung Suh M, Ohlrogge JB (2000) Stearoyl-acyl carrier protein and unusual acyl-acyl carrier protein desaturase activities are differentially influences by ferredoxin. Plant Physiol 124:681-692
27. Shanklin J, Cahoon EB (1998) Desaturation and related modifications of fatty acids. Annu Rev Plant Physiol Plant Mol Biol 49:611-641
28. Shanklin J, Whittle E, Fox BG (1994) Eight histidine residues are catalytically essential in a membrane-associated iron enzyme, steroyl-CoA desaturase and are conserved in alkene hydroxylase and xylene monooxygenase. Biochemistry 33:12787-12794
29. Sperling P, Ternes P, Zank TK, Heinz E (2003) The evolution of desaturases. Prostaglandins Leukot Essent Fatty Acids 68:73-95
30. Sundberg RJ, Martin RB (1974) Interactions of histidine and other imidazole derivatives with transition metal ions in chemical and biological systems. Chem Rev 74:471-517
31. Ta TD, Vickery LE (1992) Cloning, sequencing and overexpression of a [2Fe-2S] ferredoxin gene from Escherichia coli. J Biol Chem 267:11120-11125
32. Tocher DR, Leaver MJ, Hodgson PA (1998) Recent advances in the biochemistry and molecular biology of fatty acyl desaturases. Prog Lipid Res 37:73-117
33. Wada H, Avelange-Macherel M, Murata N (1993) The desA gene of the cyanobacterium Synechocystis sp. strain PCC 6803 is the structural gene for Δ12 desaturase. J Bacteriol 175:6056-6058
34. Whittle E, Shanklin J (2001) Engineering delta9-16:0-acyl carrier protein (ACP) desaturase specificity based on combinatorial saturation mutagenesis and logical redesign of the castor delta9-18:0-ACP desaturase. Biol Chem 276:21500-21505
35. Zámocky M, Gunther R, Jakopitsch C, Obinger C (2001) The molecular peculiarities of catalase-peroxidase. FEBS 492:177-182