Benzylpenicyllin and acetylcysteine protection from α-amanitin-induced apoptosis in human hepatocyte cultures

Experimental and Toxicologic Pathology

Volumn 63, Issue 4, 2011, Pages 311-315

  Magdalan, Jan ^a   Piotrowska, Aleksandra ^a   Gomułkiewicz, Agnieszka ^a   Sozański, Tomasz ^a   Podhorska Okołów, Marzena ^a   Szelag, Adam ^a   Dziegiel, Piotr ^a,b  

^a WROCLAW MEDICAL UNIVERSITY   (Poland)

^b POZNAN UNIVERSITY OF MEDICAL SCIENCES   (Poland)

Author keywords

Amanitin; Acetylcysteine; Amanita phalloides; Apoptosis; Benzylpenicillin; Human hepatocytes; Intoxication

Indexed keywords

ACETYLCYSTEINE; ALPHA AMANITIN; CASPASE 3; LIPOCORTIN 5; PENICILLIN G; PROTEIN BCL 2; PROTEIN P53;

APOPTOSIS; ARTICLE; CELL PROTECTION; CELL VIABILITY; CONTROLLED STUDY; CYTOTOXICITY; DNA FRAGMENTATION; ELECTRON MICROSCOPY; ENZYME ACTIVITY; HUMAN; HUMAN CELL; LIVER CELL CULTURE; LIVER PROTECTION; MUSHROOM POISONING;

ACETYLCYSTEINE; ALPHA-AMANITIN; ANTIDOTES; APOPTOSIS; CELL SURVIVAL; CELLS, CULTURED; DNA FRAGMENTATION; ENZYME-LINKED IMMUNOSORBENT ASSAY; HEPATOCYTES; HUMANS; MICROSCOPY, ELECTRON, TRANSMISSION; PENICILLIN G;

AMANITA PHALLOIDES;

EID: 79953740363     PISSN: 09402993     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.etp.2010.02.004     Document Type: Article

References (30)

1. Andera L, Wasylyk B Transcription abnormalities potentiate apoptosis of normal human fibroblasts. Mol Med 1997, 12:852-863.
2. Arima Y, Nitta M, Kuninaka S, Zhang D, Fujiwara T, Taya Y, et al. Transcriptional blockade induces p53-dependent apoptosis associated with translocation of p53 to mitochondria. J Biol Chem 2005, 280:19166-19176.
3. Aruoma OI, Halliwell B, Hoey BM, Butler J The antioxidant action of N-acetylcysteine: its reaction with hydrogen peroxide, hydroxyl radical, superoxide, and hypochlorous acid. Free Radic Biol Med 1989, 6:593-597.
4. Blankenberg FG In vivo detection of apoptosis. JNM 2008, 49:81-95.
5. Boersma HH, Kietselaer BLJH, Stolk LML, Bennaghmouch A, Hofstra L, Narula J, et al. Past, present, and future of annexin A5: from protein discovery to clinical applications. JNM 2005, 46:2035-2050.
6. Busnell DA, Cramer P, Kornberg RD Structural basis of transcription: alpha-amanitin-RNA polymerase II cocrystal at 2.8 A resolution. Proc Natl Acad. Sci USA 2002, 99:1218-1222.
7. Caelles C, Helmberg A, Karin M p53-Dependent apoptosis in the absence of transcriptional activation of p53-target genes. Nature 1994, 370:220-223.
8. Cohen GM Caspases: the executioners of apoptosis. Biochem J 1997, 326:1-16.
9. Ellenhorn M Ellenhorn's medical toxicology. Diagnosis and treatment of human poisoning 1997, Williams & Wilkins, Baltimore.
10. Enjalbert A, Rapior S, Nouguier-Soule J, Guillon S, Amoroux N, Cabot C Treatment of amatoxin poisoning: 20-year retrospective analysis. J Toxicol Clin Toxicol 2002, 40:715-757.
11. Escudié L, Francoz C, Vinel JP, Moucari R, Cournot M, Paradis V, et al. Amanita phalloides poisoning: reassessment of prognostic factors and indications for emergency liver transplantation. J Hepatol 2007, 46:466-473.
12. Fineschi V, Di Paolo M, Centini F Histological criteria for diagnosis of Amanita phalloides poisoning. J Forensic Sci 1996, 41:429-432.
13. Gartel AL Transcriptional inhibitors, p53 and apoptosis. Biochim Biophys Acta 2008, 1786:83-86.
14. Haupt Y, Rowan S, Shaulian E, Vousden KH, Oren M Induction of apoptosis in HeLa cells by trans-activation-deficient p53. Genes Dev 1995, 9:2170-2183.
15. Heilmeyer W, Plempel M, Otten H Antibiotika-Fibel. Antibiotika und Chemotherapie. 3 Auflage 1969, Georg Thieme Verlag, Stuttgart.
16. Jan MA, Siddiqui TS, Ahmed N, Haq I, Khan Z Mushroom poisoning in children: clinical presentation and outcome. J Ayub Med Coll Abottabad 2008, 20:99-101.
17. Letschert K, Faulstich H, Keller D, Keppler D Molecular characterization and inhibition of amanitin uptake into human hepatocytes. Toxicol Sci 2006, 91:140-149.
18. Magdalan J, Ostrowska A, Piotrowska A, Gomułkiewicz A, Podhorska-Okołów M, Patrzałek D, et al. Benzylpenicillin, acetylcysteine and silibinin as antidotes in human hepatocytes intoxicated with α-amanitin. Exp Toxicol Pathol, in press, doi:10.1016/j.etp.2009.05.03.
19. Magdalan J, Ostrowska A, Piotrowska A, Gomułkiewicz A, Szela{ogonek}g A, Dzie{ogonek}giel P. Comparative antidotal efficacy of benzylpenicyllin, ceftazidime and rifamycin in cultured human hepatocytes intoxicated with α-amanitin. Arch Toxicol 2009, 83:1091-1096.
20. Magdalan J, Ostrowska A, Podhorska-Okołów M, Piotrowska A, Iżykowska I, Nowak M, et al. Early morphological and functional alterations in canine hepatocytes due to α-amanitin, a major toxin of Amanita phalloides. Arch Toxicol 2009, 83:55-60.
21. Magdalan J, Pieśniewska M, Gliniak H The inhibition of α-amanitin uptake in perfused rat liver. Adv Clin Exp Med 2007, 16:355-362.
22. Moldeus P, Cotgreave IA, Berggren M Lung protection by a thiol-containing antioxidant: N-acetylcysteine. Respiration 1986, 50:31-42.
23. Munoz LE, Franz S, Pausch F, Furnrohr B, Sheriff A, Vogt B, et al. The influence on the immunomodulatory effects of dying and dead cells of Annexin V. J Leukoc Biol 2007, 81:6-14.
24. Nguyen VT, Giannoni F, Dubois MF, Seo SJ, Vigneron M, Kedinger C, et al. In vivo degradation of RNA polymerase II largest subunit triggered by alpha-amanitin. Nucleic Acids Res 1996, 24:2924-2929.
25. Park JR, Hockenbery DM BCL-2, a novel regulator of apoptosis. J Cell Biochem 1996, 60:12-17.
26. Prescott LF, Donovan JW, Jarvie DR, Proudfoot AT The disposition and kinetics of intravenous N-acetylcysteine in patients with paracetamol overdosage. Eur J Clin Pharmacol 1989, 37:501-506.
27. Reed JC, Miyashita T, Takayama S, Wang HG, Sato T, Krajewski S, et al. BCL-2 family proteins - regulators of cell death involved in the pathogenesis of cancer and resistance to therapy. J Cell Biochem 1996, 60:23-32.
28. Schneider SM Mushrooms. Clinical toxicology 2001, 899-909. Saunders Company, Philadelphia, London, New York, St. Louis, Sydney, Toronto. M.D. Ford, K.A. Delaney, L.J. Ling, W.B. Erickson (Eds.).
29. Smrz D, Lebduska P, Draberova L, Korb J, Draber P Engagement of phospholipid scramblase 1 in activated cells; Implication for phosphatidylserine externalization and exocytosis. J Biol Chem. 2008, 283:10904-10918.
30. Wolf BB, Green DR Suicidal tendencies: apoptotic cell death by caspase family proteinases. J Biol Chem 1999, 274:20049-20052.