Multiple-Catalytic Sensing of Nucleic Acid Sequences by Utilising a DNA-RNA-DNA Chimeric Antisense Probe and RNase H with a Eukaryotic Cell-Free Translation System

ChemBioChem

Volumn 12, Issue 6, 2011, Pages 881-885

  Ogawa, Atsushi ^a  

^a EHIME UNIVERSITY   (Japan)

Author keywords

Antisense DNA; Cell free translation; Cycling probe technology; Nucleic acids; Sensors

Indexed keywords

ANTISENSE OLIGONUCLEOTIDE; CHEMOKINE RECEPTOR CCR5; CHIMERIC ANTIBODY; DNA POLYMERASE; RIBONUCLEASE H;

ARTICLE; BINDING SITE; CATALYST; CHEMOLUMINESCENCE; DISEASE ASSOCIATION; DISEASE MARKER; EUKARYOTIC CELL; GENE SEQUENCE; HUMAN IMMUNODEFICIENCY VIRUS 1; IN VIVO STUDY; MOLECULAR BEACON; NUCLEIC ACID AMPLIFICATION; NUCLEIC ACID PROBE; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RNA TRANSLATION; SINGLE NUCLEOTIDE POLYMORPHISM; WHEAT GERM;

BASE SEQUENCE; CATALYSIS; CELL-FREE SYSTEM; DNA, ANTISENSE; FLUORESCENT DYES; NUCLEIC ACID AMPLIFICATION TECHNIQUES; PLANT EXTRACTS; PROTEIN BIOSYNTHESIS; RIBONUCLEASE H; RNA PROBES; RNA, MESSENGER; TRITICUM;

EUKARYOTA;

EID: 79953685892     PISSN: 14394227     EISSN: 14397633     Source Type: Journal    
DOI: 10.1002/cbic.201000744     Document Type: Article

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32. 3 cps) can be detected, this translation efficiency is not necessarily low.
33. This CL ratio hence represents the suppression efficiency of translation.
34. full (ten- and 33 times smaller than the probe, respectively) can be detected with CL ratios of 5 and 2.5, respectively, by using 1 U of RNase H.
35. full can be detected with a CL ratio of 41 under the same conditions.
36. [13]
37. However, the method did not work well in the detection of the target in human serum (no translation occurred in the absence of the target, probably as a consequence of mRNA degradation). This suggests that samples must be cleaned before detection.
38. 1 fmol mismatched target (1misA, 1misT, or 2mis) showed no translation suppression (CL ratio=1.0) under the same conditions.
39. [17] This can be attributed to the probe cleaving the mRNA without the target, given the fact that the larger amount of RNase H had almost no effect on translation without the probe (data not shown).
40. full can be detected with a CL ratio of 24 by using 16 U of RNase H. This is 1.5-fold higher than that when using 8 U of RNase H.
41. 2=0.998).
42. A larger amount of target might be detected with shorter incubation time.