Doubly catalytic sensing of HIV-1-related CCR5 sequence in prokaryotic cell-free translation system using riboregulator-controlled luciferase activity

Journal of the American Chemical Society

Volumn 127, Issue 15, 2005, Pages 5300-5301

  Sando, Shinsuke ^a   Narita, Atsushi ^a   Abe, Kenji ^a   Aoyama, Yasuhiro ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CHEMOKINE RECEPTOR CCR5; LUCIFERASE;

AMINO ACID SEQUENCE; ARTICLE; CATALYSIS; CELL FREE SYSTEM; ENZYME ACTIVITY; HUMAN IMMUNODEFICIENCY VIRUS 1; PROKARYOTIC CELL;

BASE SEQUENCE; CATALYSIS; CELL-FREE SYSTEM; CHEMILUMINESCENT MEASUREMENTS; GENE EXPRESSION REGULATION; HIV-1; HUMANS; LUCIFERASES; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; PROTEIN BIOSYNTHESIS; RECEPTORS, CCR5; RNA PROBES; RNA, MESSENGER;

EID: 17644417762     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja0507057     Document Type: Article

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22. The anti-RBS site in the MB domain is followed by a 23 nt stem-forming sequence often added to improve the stability of the transcribed RNA. The template dsDNA was prepared by stepwise insertion of the MB domain and the T7 promoter into the luciferase region of the pGL3 vector (Promega); first PCR (MB domain) with forward primer 5′-GGT CTG AAG GTT TAT TAG AAG GAG ATA TAC CAA TGG AAG ACG CCA AAA ACA TA-3′ and reverse primer 5′-TAT TCA TTA CAC GGC GAT CTT TCC G-3′ and second PCR (T7 promoter) with forward primer 5′-GAA ATT AAT ACG ACT CAC TAT AGG GAG ACC ACA ACG GTT TCC CTC TAT CTC CTG GTC TGA AGG TTT A-3′ and reverse primer 5′-TAT TCA TTA CAC GGC GAT CTT TCC G-3′. The first and the second PCR products were purified by gel electrophoresis. The riboregulator mRNA (total 1722 nt) was transcribed using MegaShortScript (Ambion) and purified by the RNeasy kit (Qiagen).
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27. The hairpin-locked structure would be generally stabilized by a longer stem and destabilized by the presence of an intrastrand anti-(anti-RBS) site and also by helicase activity, if any, in the case of in-cell sensing. Contrary to simple MBs, the present riboregulator is a big molecule, where such factors as mentioned above should be optimized as a whole to give a high level of allosteric (on/off) factor.
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