Antibody-free peptide substrate screening of serine/threonine kinase (protein kinase A) with a biotinylated detection probe

Analytical Biochemistry

Volumn 413, Issue 1, 2011, Pages 30-35

  Kim, Mira ^a   Park, Yong Sun ^a   Shin, Dong Sik ^a   Kim, Jaehi ^a   Kim, Byung Gee ^a   Lee, Yoon Sik ^a  

^a Seoul National University   (South Korea)

Author keywords

Combinatorial chemistry; Peptide library; Phospho peptide; Ser Thr kinase; Elimination

Indexed keywords

ADDITION REACTIONS; ALKALINITY; AMINO ACIDS; ANTIBODIES; BIOCHIPS; CHEMICAL MODIFICATION; COENZYMES; PEPTIDES; PHOTOLYSIS; POSITIVE IONS; PROBES; RESINS; SUBSTRATES;

ALKALINE PHOSPHATE; COMBINATORIAL CHEMISTRY; KINASE SUBSTRATES; MICHAEL ADDITIONS; PEPTIDE FRAGMENTS; PEPTIDE LIBRARY; SER/THR KINASE; THREONINE RESIDUES;

CHEMICAL DETECTION;

CYCLIC AMP DEPENDENT PROTEIN KINASE; PEPTIDE FRAGMENT; RESIN; SERINE; THREONINE;

ANTIBODY DETECTION; ARTICLE; BIOTINYLATION; CHEMICAL MODIFICATION; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; PHOTOLYSIS; PRIORITY JOURNAL; PROTEIN SECRETION;

EID: 79953681012     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2011.02.005     Document Type: Article

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