The mechanism of PNIPAAm-assisted refolding of lysozyme denatured by urea

Biochemical Engineering Journal

Volumn 24, Issue 1, 2005, Pages 55-64

  Lu, Diannan ^a   Liu, Zhixia ^a   Zhang, Minlian ^a   Liu, Zheng ^a   Zhou, Haimeng ^a  

^a TSINGHUA UNIVERSITY   (China)

Author keywords

Folding kinetics; Lysozyme; Poly(N isopropyl acylamide); Protein downstream process; Refolding in vitro

Indexed keywords

EMISSION SPECTROSCOPY; FLUORESCENCE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROPHOBICITY; MOLECULAR WEIGHT; POLYACRYLATES; PROTEINS; UREA;

CIRCULAR DICHROISM (CD); FLUORESCENCE EMISSION SPECTROSCOPY; LYSOZYMES; POLY-N-ISOPROPYL ACRYLAMIDE (PNIPAAM);

ENZYMES;

ACRYLAMIDE DERIVATIVE; BUFFER; LYSOZYME; POLY(N ISOPROPYLACRYLAMIDE}; POLYMER; UNCLASSIFIED DRUG; UREA;

ARTICLE; CHEMICAL STRUCTURE; CIRCULAR DICHROISM; DENATURATION; DILUTION; DISSOCIATION; ENZYME ACTIVITY; FLUORESCENCE SPECTROSCOPY; HYDROPHOBICITY; INTERMETHOD COMPARISON; KINETICS; MOLECULAR INTERACTION; MOLECULAR WEIGHT; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN FOLDING; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; TEMPERATURE;

EID: 17444385593     PISSN: 1369703X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bej.2004.12.002     Document Type: Article

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