Efficient generation of unmarked deletions in Legionella pneumophila

Applied and Environmental Microbiology

Volumn 77, Issue 7, 2011, Pages 2545-2548

  Bryan, Andrew ^a   Harada, Kaoru ^a   Swanson, Michele S ^a  

^a UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EXONUCLEASE ACTIVITY; GENE DELETION; LEGIONELLA PNEUMOPHILA; MULTICOMPONENTS; NATURAL TRANSFORMATIONS; SITE-SPECIFIC;

GENES;

ESCHERICHIA COLI;

ALLELE; BACTERIUM; COLIFORM BACTERIUM; ENZYME ACTIVITY; GENETIC ANALYSIS; MOTILITY; PLASMID; TRANSFORMATION;

ARTICLE; BACTERIUM TRANSFORMATION; ESCHERICHIA COLI; GENE DELETION; GENE EXPRESSION REGULATION; GENETIC RECOMBINATION; GENETIC SELECTION; GENETICS; LEGIONELLA PNEUMOPHILA; METHODOLOGY; MICROBIAL GENETICS; PLASMID;

ESCHERICHIA COLI; GENETICS, MICROBIAL; LEGIONELLA PNEUMOPHILA; MUTAGENESIS, INSERTIONAL; PLASMIDS; RECOMBINATION, GENETIC; SELECTION, GENETIC; SEQUENCE DELETION; TRANSFORMATION, BACTERIAL;

ESCHERICHIA COLI; LEGIONELLA PNEUMOPHILA;

EID: 79953052795     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.02904-10     Document Type: Article

References (32)

1. Berger, K. H., and R. R. Isberg. 1993. Two distinct defects in intracellular growth complemented by a single genetic locus in Legionella pneumophila. Mol. Microbiol. 7:7-19.
2. Bryan, A., and M. S. Swanson. 2011. Oligonucleotides stimulate genomic alterations of Legionella pneumophila. Mol. Microbiol. [Epub ahead of print.] doi:10.1111/j.1365-2958.2011.07573.x.
3. Burdett, V., C. Baitinger, M. Viswanathan, S. T. Lovett, and P. Modrich. 2001. In vivo requirement for RecJ, ExoVII, ExoI, and ExoX in methyldirected mismatch repair. Proc. Natl. Acad. Sci. U. S. A. 98:6765-6770.
4. Byrne, B., and M. S. Swanson. 1998. Expression of Legionella pneumophila virulence traits in response to growth conditions. Infect. Immun. 66:3029-3034.
5. Chase, J. W., and C. C. Richardson. 1977. Escherichia coli mutants deficient in exonuclease VII. J. Bacteriol. 129:934-947.
6. Chen, D. Q., S. S. Huang, and Y. J. Lu. 2006. Efficient transformation of Legionella pneumophila by high-voltage electroporation. Microbiol. Res. 161: 246-251.
7. Chien, M., et al. 2004. The genomic sequence of the accidental pathogen Legionella pneumophila. Science 305:1966-1968.
8. Cianciotto, N. P. 2009. Many substrates and functions of type II secretion: lessons learned from Legionella pneumophila. Future Microbiol. 4:797-805.
9. Court, D. L., J. A. Sawitzke, and L. C. Thomason. 2002. Genetic engineering using homologous recombination. Annu. Rev. Genet. 36:361-388.
10. Cox, M. M. 1983. The FLP protein of the yeast 2-microns plasmid: expression of a eukaryotic genetic recombination system in Escherichia coli. Proc. Natl. Acad. Sci. U. S. A. 80:4223-4227.
11. Datsenko, K. A., and B. L. Wanner. 2000. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U. S. A. 97:6640-6645.
12. Dutra, B. E., V. A. Sutera, Jr., and S. T. Lovett. 2007. RecA-independent recombination is efficient but limited by exonucleases. Proc. Natl. Acad. Sci. U. S. A. 104:216-221.
13. Falco, S. C., Y. Li, J. R. Broach, and D. Botstein. 1982. Genetic properties of chromosomally integrated 2 mu plasmid DNA in yeast. Cell 29:573-584.
14. Golic, K. G., and S. Lindquist. 1989. The FLP recombinase of yeast catalyzes site-specific recombination in the Drosophila genome. Cell 59:499-509.
15. Hubber, A., and C. R. Roy. 2010. Modulation of host cell function by Legionella pneumophila type IV effectors. Annu. Rev. Cell Dev. Biol. 26:261-283.
16. Isberg, R. R., T. J. O'Connor, and M. Heidtman. 2009. The Legionella pneumophila replication vacuole: making a cosy niche inside host cells. Nat. Rev. Microbiol. 7:13-24.
17. Marra, A., S. J. Blander, M. A. Horwitz, and H. A. Shuman. 1992. Identification of a Legionella pneumophila locus required for intracellular multiplication in human macrophages. Proc. Natl. Acad. Sci. U. S. A. 89:9607-9611.
18. Molofsky, A. B., L. M. Shetron-Rama, and M. S. Swanson. 2005. Components of the Legionella pneumophila flagellar regulon contribute to multiple virulence traits, including lysosome avoidance and macrophage death. Infect. Immun. 73:5720-5734.
19. Molofsky, A. B., and M. S. Swanson. 2004. Differentiate to thrive: lessons from the Legionella pneumophila life cycle. Mol. Microbiol. 53:29-40.
20. Morschhäuser, J., S. Michel, and P. Staib. 1999. Sequential gene disruption in Candida albicans by FLP-mediated site-specific recombination. Mol. Microbiol. 32:547-556.
21. Newton, H. J., D. K. Ang, I. R. van Driel, and E. L. Hartland. 2010. Molecular pathogenesis of infections caused by Legionella pneumophila. Clin. Microbiol. Rev. 23:274-298.
22. O'Gorman, S., D. T. Fox, and G. M. Wahl. 1991. Recombinase-mediated gene activation and site-specific integration in mammalian cells. Science 251:1351-1355.
23. Pasculle, A. W., et al. 1980. Pittsburgh pneumonia agent: direct isolation from human lung tissue. J. Infect. Dis. 141:727-732.
24. Schweizer, H. P. 2003. Applications of the Saccharomyces cerevisiae Flp-FRT system in bacterial genetics. J. Mol. Microbiol. Biotechnol. 5:67-77.
25. Senecoff, J. F., R. C. Bruckner, and M. M. Cox. 1985. The FLP recombinase of the yeast 2-micron plasmid: characterization of its recombination site. Proc. Natl. Acad. Sci. U. S. A. 82:7270-7274.
26. Sexton, J. A., and J. P. Vogel. 2004. Regulation of hypercompetence in Legionella pneumophila. J. Bacteriol. 186:3814-3825.
27. Stone, B. J., and Y. A. Kwaik. 1999. Natural competence for DNA transformation by Legionella pneumophila and its association with expression of type IV pili. J. Bacteriol. 181:1395-1402.
28. Thomason, L., et al. 2007. Recombineering: genetic engineering in bacteria using homologous recombination. Curr. Protoc. Mol. Biol. 1:1.16.
29. Thomason, L. C., N. Costantino, D. V. Shaw, and D. L. Court. 2007. Multicopy plasmid modification with phage lambda Red recombineering. Plasmid 58:148-158.
30. Viswanathan, M., V. Burdett, C. Baitinger, P. Modrich, and S. T. Lovett. 2001. Redundant exonuclease involvement in Escherichia coli methyl-directed mismatch repair. J. Biol. Chem. 276:31053-31058.
31. Viswanathan, M., and S. T. Lovett. 1998. Single-strand DNA-specific exonucleases in Escherichia coli. Roles in repair and mutation avoidance. Genetics 149:7-16.
32. Yu, D., et al. 2000. An efficient recombination system for chromosome engineering in Escherichia coli. Proc. Natl. Acad. Sci. U. S. A. 97:5978-5983.