Efficient transformation of Legionella pneumophila by high-voltage electroporation

Microbiological Research

Volumn 161, Issue 3, 2006, Pages 246-251

  Chen, Ding Qiang ^a   Huang, Shao Song ^a   Lu, Yong Jun ^a  

^a School of Life Sciences   (China)

Author keywords

Electroporation; High voltage; Legionella pneumophila; Transformation

Indexed keywords

CELLS; CLONING; DNA; ELECTRIC FIELDS; ELECTRIC POTENTIAL;

ELECTROPORATION; HIGH VOLTAGE; LEGIONELLA PNEUMOPHILA; TRANSFORMATION;

MOLECULAR BIOLOGY;

BACTERIUM; DNA; ELECTRIC FIELD; GENE TRANSFER; MUTATION; PLASMID; TRANSFORMATION;

LEGIONELLA PNEUMOPHILA;

EID: 33744809269     PISSN: 09445013     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.micres.2005.09.001     Document Type: Article

References (20)

1. Barker J., Brown M.R., Collier P.J., Farrell I., and Gisbert P. Relationship between Legionella pneumophila and Acanthamoeba polyphaga: physiological status and susceptibility to chemical inactivation. Appl. Environ. Microbiol. 58 (1992) 2420-2425
2. Chen G.C.C., Lema M., and Brown A. Plasmid transfer into members of the family Legionellaceae. J. Infect. Dis. 150 (1984) 513-516
3. Cianciotto N.P., and Fields B.S. Legionella pneumophila mip gene potentiates intracellular infection of protozoa and human macrophages. Proc. Natl. Acad. Sci. 89 (1992) 5188-5191
4. Dower W.J., Miller J.F., and Ragsdale W.S. High efficiency transformation of E. coli by high-voltage electroporation. Nucleic Acids Res. 16 (1988) 6127-6145
5. Dower W.J., Chassy B.M., Trevors J.T., and Blaschek H.P. Protocols for the transformation of bacteria by electroporation. In: Chang D.C., Chassy B.M., Saunders J.A., and Sowers A.E. (Eds). Guide to Electroporation and Electrofusion (1992), Academic Press, San Diego 485-499
6. Edelstein P.H. Improved semiselective medium for isolation of Legionella pneumophila from contaminated clinical and environmental specimens. J. Clin. Microbiol. 14 (1981) 298-303
7. Fliermanns C.B. Ecology of Legionella: from data to knowledge with a little wisdom. Microb. Ecol. 32 (1996) 203-228
8. Fournet-Fayard S., Joly B., and Forestier C. Transformation of wild type Klebsiella pneumoniae with plasmid DNA by electroporation. J. Microbiol. Methods 24 (1995) 49-54
9. Jore J.P.M., van Luijk N., Luiten R.G.M., van der Werf M.J., and Pouwels P.H. Efficient transformation system for Propionibacterium freudenreichii based on a novel vector. Appl. Environ. Microbiol. 67 (2001) 499-503
10. Mack K., and Titball R.W. Transformation of Burkholderia pseudomallei by electroporation. Anal. Biochem. 242 (1996) 73-76
11. Marra A., Blander S.J., Horwitz M.A., and Shuman H.A. Identification of a Legionella pneumophila locus required for intracellular multiplication in human macrophages. Proc. Natl. Acad. Sci. 89 (1992) 9607-9611
12. Miller J.F., Dower W.J., and Tompkins L.S. High-voltage electroporation of bacteria: genetic transformation of Campylobacter jejuni with plasmid DNA. Proc. Natl. Acad. Sci. 85 (1988) 856-860
13. Miller W.G., Bates A.H., Horn S.T., Brandl M.T., Wachtel M.R., and Mandrell R.E. Detection on surfaces and in Caco-2 cells of Campylobacter jejuni cells transformed with new gfp, yfp and cfp marker plasmids. Appl. Environ. Microbiol. 66 (2000) 5426-5436
14. Mintz C.S. Gene transfer in Legionella pneumophila. Microb. Infect 1 (1999) 1203-1209
15. Pigac J., and Schrempf H. A simple and rapid method of transformation of Streptomyces rimosus R6 and other Streptomycetes by electroporation. Appl. Environ. Microbiol. 61 (1995) 352-356
16. Sexton J.A., and Vogel J.P. Regulation of hypercompetence in Legionella pneumophila. J. Bacteriol. 186 (2004) 3814-3825
17. Sonesson A., Jantzen E., Bryn K., Larsson L., and Eng J. Chemical composition of a lipopolysaccharide from Legionella pneumophila. Arch. Microbiol. 153 (1989) 72-78
18. Stone B.J., and Abu-kwaik Y. Natural competence for DNA transformation by Legionella pneumophila and its association with expression of type IV pili. J. Bacteriol. 183 (1999) 1395-1402
19. Vogel P.J., and Isberg R.R. Cell biology of Legionella pneumophila. Curr. Opin. Microbiol. 2 (1999) 30-34
20. Xue G.P., Johnson J.S., and Dalrymple B.P. High osmolarity improves the electro-transformation efficiency of the Gram-positive bacteria Bacillus subtilis and Bacillus licheniformis. J. Microbiol. Methods 34 (1999) 183-191