Bacterial expression, purification, and model membrane reconstitution of the transmembrane and cytoplasmic domains of the human APP binding protein LR11/SorLA for NMR studies

Protein Expression and Purification

Volumn 77, Issue 2, 2011, Pages 224-230

  Wang, Xingsheng ^a   Gill Jr , Richard L ^a   Zhu, Qin ^a   Tian, Fang ^a  

^a PENN STATE COLLEGE OF MEDICINE   (United States)

Author keywords

Alzheimer's disease; Amyloid precursor protein binding protein; LR11; Membrane protein overexpression; SorLA

Indexed keywords

AMYLOID PRECURSOR PROTEIN; CARRIER PROTEIN; DETERGENT; HIS HIS HIS HIS HIS HIS; HIS-HIS-HIS-HIS-HIS-HIS; HISTIDINE; HYBRID PROTEIN; LOW DENSITY LIPOPROTEIN RECEPTOR RELATED PROTEIN; MEMBRANE PROTEIN; OLIGOPEPTIDE; PROTEINASE; SORL1 PROTEIN, HUMAN; TEV PROTEASE; THROMBIN;

AGED; ALZHEIMER DISEASE; AMINO ACID SEQUENCE; ANIMAL; ARTICLE; CHEMISTRY; ESCHERICHIA COLI; GENE VECTOR; GENETICS; HUMAN; METABOLISM; MICELLE; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PROTEIN TERTIARY STRUCTURE;

AGED; ALZHEIMER DISEASE; AMINO ACID SEQUENCE; AMYLOID BETA-PROTEIN PRECURSOR; ANIMALS; CLONING, MOLECULAR; DETERGENTS; ENDOPEPTIDASES; ESCHERICHIA COLI; GENETIC VECTORS; HISTIDINE; HUMANS; LDL-RECEPTOR RELATED PROTEINS; MAGNETIC RESONANCE SPECTROSCOPY; MEMBRANE PROTEINS; MEMBRANE TRANSPORT PROTEINS; MICELLES; MOLECULAR SEQUENCE DATA; OLIGOPEPTIDES; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS; THROMBIN;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; TOBACCO ETCH VIRUS;

EID: 79952631532     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2011.02.004     Document Type: Article

References (46)

1. S.A. Small, and S. Gandy Sorting through the cell biology of Alzheimer's disease: intracellular pathway to pathogenesis Neuron 52 2006 15 31 (Pubitemid 44466354)
2. O.M. Andersen, J. Reiche, V. Schmidt, M. Gotthardt, R. Spoelgen, J. Behlke, C.A.F. Arnim, T. Breiderhoff, P. Jansen, X. Wu, K.R. Bales, R. Cappai, C.L. Masters, J. Gliemann, E.J. Mufson, B.T. Hyman, S.M. Paul, A. Nykjaer, and T.E. Willnow Neuronal sorting protein-related receptor sorLA/LR11 regulates processing of the amyloid precursor protein Proc. Natl. Acad. Sci. USA 102 2005 13461 13466 (Pubitemid 41420870)
3. T.E. Willnow, C.M. Petersen, and A. Nykjaer VPS10P-domain receptors - regulators of neuronal viability and function Nat. Rev. Neurosci. 9 2008 899 909
4. W.P. Esler, and M.S. Wolfe A protrait of Alzheimer secretases - new features and familiar faces Science 293 2001 1449 1454 (Pubitemid 32801541)
5. R. Vassar, and M. Citron A β-generating enzymes: recent advances in β- and γ-secretase research Neuron 27 2000 419 422
6. R. Yan, M.J. Bienkowski, M.E. Shuck, H. Miao, M.C. Tory, A.M. Pauley, J.R. Brashler, N.C. Stratman, W.R. Mathews, A.E. Buhl, D.B. Cater, A. Tomasselli, L.A. Parodi, R.L. Heinrikson, and M.E. Gurney Membrane-anchored aspartyl protease with Alzheimer's disease β-secretase activity Nature 402 1999 533 537
7. R. Vassar, B.D. Bennett, S. Babu-Khan, S. Kahn, E. Mendiaz, P. Denis, D.B. Teplow, S. Ross, P. Amarante, R. Leoeloff, Y. Lou, S. Fisher, J. Fuller, S. Edenson, J. Lile, M.A. Jarosinski, A.J. Biere, E. Curran, T. Burgess, J. Louis, F. Collins, J. Treanor, G. Rogers, and M. Citron β-Secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE Science 286 1999 735 741
8. J.A. Hardy, and G.A. Higgins Alzheimer's disease: the amyloid cascade hypothesis Science 256 1992 184 185
9. R.E. Tanzi, and L. Bertram Twenty years of the Alzheimer's disease amyloid hypothesis: a genetic perspective Cell 120 2005 545 555 (Pubitemid 40269768)
10. D.J. Selkoe The ups and downs of A beta Nat. Med. 12 2006 758 759
11. K.A. Jellinger Alzheimer 100 - highlights in the history of Alzheimer research J. Neural Transm. 113 2006 1603 1623 (Pubitemid 44660049)
12. R. Jakob-Roetne, and H. Jacobsen Alzheimer's disease: from pathology to therapeutic approaches Angew. Chem. Int. Ed. 48 2009 3030 3059
13. D.J. Selkoe Alzheimer's disease: genes, protein, and therapy Physiol. Rev. 81 2001 741 766 (Pubitemid 32267077)
14. S.E. Dodson, M. Gearing, C.F. Lippa, T.J. Montine, A.I. Levey, and J.J. Lah LR11/SorLA expression is reduced in sporadic Alzheimer disease but not in familial Alzheimer disease J. Neuropathol. Exp. Neurol. 65 2006 866 872 (Pubitemid 44371232)
15. K. Offe, S.E. Dodson, J.T. Shoemaker, J.J. Fritz, M. Gearing, A.I. Levey, and J.J. Lah The lipoprotein receptor LR11 regulates amyloid b production and amyloid precursor protein traffic in endosomal compartments J. Neurosci. 26 2006 1596 1603 (Pubitemid 43237030)
16. E. Rogaeva, Y. Meng, J.H. Lee, Y.J. Gu, T. Kawarai, F.G. Zou, T. Katayama, C.T. Baldwin, R. Cheng, H. Hasegawa, F.S. Chen, N. Shibata, K.L. Lunetta, R. Pardossi-Piquard, C. Bohm, Y. Wakutani, L.A. Cupples, K.T. Cuenco, R.C. Green, L. Pinessi, I. Rainero, S. Sorbi, A. Bruni, R. Duara, R.P. Friedland, R. Inzelberg, W. Hampe, H. Bujo, Y.Q. Song, O.M. Andersen, T.E. Willnow, N. Graff-Radford, R.C. Petersen, D. Dickson, S.D. Der, P.E. Fraser, G. Schmitt-Ulms, S. Younkin, R. Mayeux, L.A. Farrer, and P.S. George-Hyslop The neuronal sortilin-related receptor SORL1 is genetically associated with Alzheimer disease Nat. Genet. 2 2007 168 177 (Pubitemid 46184347)
17. R. Mayeux, and P.S.G. Hyslop Alzheimer's disease: advances in trafficking Neurology 7 2008 2 3 (Pubitemid 350258699)
18. R. Spoelgen, C.A.F. Von Arnim, A.V. Thomas, I.D. Peltan, M. Koker, A. Deng, M.C. Irizarry, D.E. Anderson, T.E. Willnow, and B.T. Hyman Interaction of the cytosolic domains of sorLA/LR11 with the amyloid precursor protein (APP) and beta-secretase beta-site APP-cleaving enzyme J. Neurosci. 26 2006 418 428 (Pubitemid 43237105)
19. J. Jacob, P. Madsen, C. Jacobsen, M.S. Nielsen, J. Gliemann, and C.M. Petersen Activation and functional characterization of the mosaic receptor SorLA/LR11 J. Biol. Chem. 276 2001 222788 222796
20. H. Yamazaki, H. Bujo, J. Kusunoki, K. Seimiya, T. Kanaki, N. Morisaki, W.J. Schneider, and Y. Saito Elements of neural adhesion molecules and a yeast vacuolar protein sorting receptor are present in a novel mammalian low density lipoprotein receptor family member J. Biol. Chem. 271 1996 24761 24768 (Pubitemid 26333222)
21. R.T. Youker, U. Shinde, R. Day, and G. Thomas At the crossroads of homoeostasis and disease: roles of the PACS proteins in membrane traffic and apoptosis Biochem. J. 421 2009 1 15
22. M.S. Nielsen, C. Gustafsen, P. Madsen, J.R. Nyengaard, G. Hermey, O. Bakke, M. Mari, P. Schu, R. Pohlmann, A. Dennes, and C.M. Petersen Sorting by the cytoplasmic domain of the amyloid precursor protein binding receptor SorLA Mol. Cell. Biol. 27 2007 6842 6851 (Pubitemid 47483634)
23. L. Jacobsen, P. Madsen, M.S. Nielsen, W.P.M. Geraerts, J. Gliemann, A.B. Smit, and C.M. Petersen The sorLA cytoplasmic domain interacts with GGA1 and -2 and defines minimum requirements for GGA binding FEBS Lett. 511 2002 155 158 (Pubitemid 34127841)
24. V. Schmidt, A. Sporbert, M. Rohe, T. Reimer, A. Rehm, O.M. Andersen, and T.E. Willnow SorLA/LR11 regulates processing of amyloid precursor protein via interaction with adaptors GGA and PACS-1 J. Biol. Chem. 282 2007 32956 32964 (Pubitemid 350159299)
25. O.M. Andersen, V. Schmidt, R. Spoelgen, J. Gliemann, J. Behlke, D. Galatis, W.J. McKinstry, M.W. Parker, C.L. Masters, B.T. Hyman, R. Cappai, and T.E. Willnow Molecular dissection of the interaction between amyloid precursor protein and its neuronal trafficking receptor SorLA/LR11 Biochemistry 45 2006 2618 2628
26. C. Bohm, N.M. Seibel, B. Henkel, H. Steiner, C. Haass, and W. Hampe SorLA signaling by regulated intramembrane proteolysis J. Biol. Chem. 281 2006 14533 14547
27. A.C. Nyborg, T.B. Ladd, C.W. Zwizinski, and T.E. Golde Sortilin, SorCS1b and SorLA Vps10p sorting receptors, are novel γ-secretase substrates Mol. Neurodegener. 1 2006 3
28. F. Junge, B. Schneider, S. Reckel, D. Schwarz, V. Dotsch, and F. Bernhard Large-scale production of functional membrane proteins Cell. Mol. Life Sci. 65 2008 1729 1755
29. H.J. Qin, J. Hu, Y.Z. Hua, S.V. Challa, T.A. Cross, and F.P. Gao Construction of a series of vectors for high throughput cloning and expression screening of membrane proteins from Mycobacterium tuberculosis BMC Biotechnol. 8 2008 51
30. A. Korepanova, J.D. Moore, H.B. Nguyen, Y. Hua, T.A. Cross, and F. Gao Expression of membrane proteins from Mycobacterium tuberculosis in Escherichia coli as fusions with maltose binding protein Protein Express. Purif. 53 2007 24 30 (Pubitemid 46283455)
31. D. Nietlispach, Y. Ito, and E.D. Laue A novel approach for the sequential backbone assignment of large proteins: selective intra-HNCA and DQ-HNCA J. Am. Chem. Soc. 124 2002 11199 11207 (Pubitemid 35025679)
32. G. Cornilescu, F. Delaglio, and A. Bax Protein backbone angle restraints from searching a database for chemical shift and sequence homology J. Biomol. NMR 13 1999 289 302 (Pubitemid 29143535)
33. K. Seimiya, S. Moerwald, H. Bujo, H. Yamazaki, J. Kusunoki, T. Kanaki, N. Morisaki, J. Nimpf, W.J. Schneider, and Y. Saito Identification and molecular characterization of LR11 from man and chicken Atherosclerosis 134 1997 362
34. J.F. Cramer, C. Gustafsen, M.A. Behrens, C.L.P. Oliveira, J.S. Pedersen, P. Madsen, C.M. Petersen, and S.S. Thirup GGA autoinhibition revisited Traffic 11 2010 259 273
35. J. Wohnert, K.J. Franz, M. Nitz, B. Imperiali, and H. Schwalbe Protein alignment by a coexpressed lanthanide-binding tag for the measurement of residual dipolar couplings J. Am. Chem. Soc. 125 2003 13338 13339 (Pubitemid 37352103)
36. M. Nitz, K.J. Franz, R.L. Maglathlin, and B. Imperiali A powerful combinatorial screen to identify high-affinity terbium(III)-binding peptides ChemBioChem 4 2003 272 276 (Pubitemid 36505197)
37. K.J. Franz, M. Nitz, and B. Imperiali Lanthanide-binding tags as versatile protein coexpression probes ChemBioChem 4 2003 265 271 (Pubitemid 36505196)
38. R.D. Krueger-Koplin, P.L. Sorgen, S.T. Krueger-Koplin, A.O. Rivera-Torres, S.M. Cahill, D.B. Hicks, L. Grinius, T.A. Krulwich, and M.E. Girvin An evaluation of detergents for NMR structural studies of membrane proteins J. Biomol. NMR 28 2004 43 57 (Pubitemid 38220159)
39. S.F. Poget, and M.E. Girvin Solution NMR of membrane proteins in bilayer mimics: small is beautiful, but sometimes bigger is better Biochim. Biophys. Acta 1768 2007 3098 3106 (Pubitemid 350236093)
40. N.J. Traaseth, R. Verardi, K.D. Torgersen, C.B. Karim, D.D. Thomas, and G. Veglia Spectroscopic validation of the pentameric structure of phospholarnban Proc. Natl. Acad. Sci. USA 104 2007 14676 14681 (Pubitemid 350003202)
41. H.J. Kim, S.C. Howell, W.D. Van Horn, Y.H. Jeon, and C.R. Sanders Recent advances in the application of solution NMR spectroscopy to multi-span integral membrane proteins Prog. NMR Spectrosc. 55 2009 335 360
42. Z.O. Shenkarev, E.N. Lyukmanova, A.S. Paramonov, L.N. Shingarova, V.V. Chupin, M.P. Kirpichnikov, M.J.J. Blommers, and A.S. Arseniev Lipid-protein nanodiscs as reference medium in detergent screening for high-resolution NMR studies of integral membrane proteins J. Am. Chem. Soc. 132 2010 5628 5629
43. T. Raschle, S. Hiller, T.Y. Yu, A.J. Rice, T. Walz, and G. Wagner Structural and functional characterization of the integral membrane protein VDAC-1 in lipid bilayer nanodiscs J. Am. Chem. Soc. 131 2009 17777 17779
44. J. Yang, Y.Q. Ma, R.C. Page, S. Misra, E.F. Plow, and J. Qin Structure of an integrin alpha IIb beta 3 transmembrane-cytoplasmic heterocomplex provides insight into integrin activation Proc. Natl. Acad. Sci. USA 106 2009 17729 17734
45. J.D. Thompson, D.G. Higgins, and T.J. Gibson CLUSTAL-W - improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice Nucleic Acids Res. 22 1994 4673 4680 (Pubitemid 24354800)
46. P. Gouet, E. Courcelle, D.I. Stuart, and F. Metoz ESPript: analysis of multiple sequence alignments in PostScript Bioinformatics 15 1999 305 308 (Pubitemid 29213756)