Efficient overexpression and purification of active full-length human transcription factor Yin Yang 1 in Escherichia coli

Protein Expression and Purification

Volumn 77, Issue 2, 2011, Pages 198-206

  Golebiowski, Filip M ^a   Górecki, Andrzej ^a   Bonarek, Piotr ^a   Dziedzicka Wasylewska, Marta ^a  

^a JAGIELLONIAN UNIVERSITY   (Poland)

Author keywords

Disulfide reduction; DNA affinity purification; mRNA secondary structure; Protein refolding; Zinc fingers

Indexed keywords

DNA; HIS HIS HIS HIS HIS HIS; HIS-HIS-HIS-HIS-HIS-HIS; HISTIDINE; OLIGOPEPTIDE; RECOMBINANT PROTEIN; TRANSCRIPTION FACTOR YY1; YY1 PROTEIN, HUMAN; ZINC FINGER PROTEIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; BINDING SITE; CELL INCLUSION; ESCHERICHIA COLI; FLUORESCENCE POLARIZATION; GEL MOBILITY SHIFT ASSAY; GENE EXPRESSION; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOLECULAR CLONING; PLASMID; PROTEIN BINDING; PROTEIN DENATURATION; PROTEIN ENGINEERING; PROTEIN REFOLDING; PROTEIN STABILITY;

BINDING SITES; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; DNA; ELECTROPHORETIC MOBILITY SHIFT ASSAY; ESCHERICHIA COLI; FLUORESCENCE POLARIZATION; GENE EXPRESSION; HISTIDINE; HUMANS; INCLUSION BODIES; OLIGOPEPTIDES; PLASMIDS; PROTEIN BINDING; PROTEIN DENATURATION; PROTEIN ENGINEERING; PROTEIN REFOLDING; PROTEIN STABILITY; RECOMBINANT PROTEINS; YY1 TRANSCRIPTION FACTOR; ZINC FINGERS;

ESCHERICHIA COLI;

EID: 79952616336     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2011.01.016     Document Type: Article

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