mRNA secondary structure at start AUG codon is a key limiting factor for human protein expression in Escherichia coli

Biochemical and Biophysical Research Communications

Volumn 349, Issue 1, 2006, Pages 69-78

  Zhang, Weici ^a   Xiao, Weihua ^a,b   Wei, Haiming ^a   Zhang, Jian ^b   Tian, Zhigang ^a,b  

^a UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA   (China)

^b SHANDONG UNIVERSITY   (China)

Author keywords

Codon optimization; E. coli; Interleukin 10; RNA stability; Secondary structure; Start codon

Indexed keywords

INTERLEUKIN 10;

ARTICLE; BASE PAIRING; CODON USAGE; ESCHERICHIA COLI; EXPRESSION VECTOR; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN SECONDARY STRUCTURE; PROTEIN TARGETING; RNA STABILITY; RNA STRUCTURE; SEQUENCE ANALYSIS; START CODON; STRUCTURE ANALYSIS; THERMODYNAMICS;

BASE SEQUENCE; CODON, INITIATOR; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENETIC TECHNIQUES; HUMANS; INTERFERON-ALPHA; INTERLEUKIN-10; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; PLASMIDS; RNA, MESSENGER;

ESCHERICHIA COLI;

EID: 33748316617     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2006.07.209     Document Type: Article

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