Overexpression of ydbK-encoding putative pyruvate synthase improves L-valine production and aerobic growth on ethanol media by an Escherichia coli strain carrying an oxygen-resistant alcohol dehydrogenase

Journal of Microbial and Biochemical Technology

Volumn 2, Issue 3, 2010, Pages 77-83

  Eremina, Natalya S ^a   Yampolskaya, Tatiana A ^a   Altman, Irina B ^a   Mashko, Sergey V ^a   Stoynova, Nataliya V ^a  

^a Ajinomoto Genetika Research Institute   (Russian Federation)

Author keywords

Alcohol dehydrogenase; Carbon dioxide fixation; Ethanol utilization; Lambda promoter mediated transcription; Reddriven chromosomal modification

Indexed keywords

ALCOHOL; ALCOHOL DEHYDROGENASE; PARAQUAT; PYRUVATE SYNTHASE; VALINE;

ALCOHOL CONSUMPTION; AMINO ACID SYNTHESIS; ARTICLE; BACTERIAL GENE; BACTERIAL GROWTH; CONTROLLED STUDY; ESCHERICHIA COLI; GENE FUNCTION; GENE INACTIVATION; GENE OVEREXPRESSION; NONHUMAN; NUCLEOTIDE SEQUENCE; OXIDATIVE STRESS; WILD TYPE; YDBK GENE;

ESCHERICHIA COLI;

EID: 79952608655     PISSN: None     EISSN: 19485948     Source Type: Journal    
DOI: 10.4172/1948-5948.1000028     Document Type: Article

References (32)

1. Akhtar MK, Jones PR (2009) Construction of a synthetic YdbK-dependent pyruvate: H2 pathway in Escherichia coli BL21 (DE3). Metab Eng, 11: 139-147.
2. Blaschkowski HP, Neuer G, Ludwig-Festl M, Knappe J (1982) Routes of flavodoxin and ferredoxin reduction in Escherichia coli. CoA-acylating pyruvate:flavodoxin and NADPH:flavodoxin oxidoreductases participating in the activation of pyruvate formate-lyase. Eur J Biochem 123: 563-569.
3. Datsenko KA, Wanner BL (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci USA 97: 6640-6645.
4. Demain AL, Adrio JL (2008) Contributions of microorganisms to industrial biology. Mol Biotechnol 38: 41-55.
5. Doroshenko V, Airich L, Vitushkina M, Kolokolova A, Livshits V, et al (2007) YddG from Escherichia coli promotes export of aromatic amino acids. FEMS Microbiol Lett 275: 312-318.
6. Furdui C, Ragsdale SW (2000) The role of pyruvate ferredoxin oxidoreductase in pyruvate synthesis during autotrophic growth by the Wood-Ljungdahl pathway. J Biol Chem 275: 28494-28499.
7. Giladi H, Igarashi K, Ishihama A, Oppenheim AB (1992) Stimulation of the phage lambda pL promoter by integration host factor requires the carboxy terminus of the alpha-subunit of RNA polymerase. J Mol Biol 227: 985-990.
8. Giladi H, Murakami K, Ishihama A, Oppenheim AB (1996) Identification of an UP element within the IHF binding site at the PL1-PL2 tandem promoter of bacteriophage lambda. J Mol Biol 260: 484-491.
9. Govorun VM, Moshkovskii SA, Tikhonova OV, Goufman EI, Serebryakova MV, et al (2003) Comparative analysis of proteome maps of Helicobacter pylori clinical isolates. Biochemistry (Mosc) 68: 42-49.
10. Katashkina JI, Skorokhodova AY, Zimenkov DV, Gulevich AY, Minaeva NI, et al (2005) [Tuning of expression level of the genes of interest located in the bacterial chromosome]. Mol Biol (Mosk) 39: 823-831.
11. Krylov AA, Airich LG, Kiseleva EM, Minaeva NI, Biryukova IV, et al (2010) Conditional silencing of the Escherichia coli pykF gene results from artificial convergent transcription protected from Rho-dependent termination. J Mol Microbiol Biotechnol 18: 1-13.
12. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
13. Liang ST, Bipatnath M, Xu YC, Chen SL, Dennis P, et al (1999) Activities of constitutive promoters in Escherichia coli. J Mol Biol 292: 19-37.
14. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265-275.
15. Menart V, Jevsevar S, Vilar M, Trobis A, Pavko A (2003) Constitutive versus thermoinducible expression of heterologous proteins in Escherichia coli based on strong PR, PL promoters from phage lambda. Biotechnol Bioeng 83: 181-190.
16. Miller JH (1972) Experiments in Molecular Genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
17. Pieulle L, Guigliarelli B, Asso M, Dole F, Bernadac A, et al (1995) Isolation and characterization of the pyruvate-ferredoxin oxidoreductase from the sulfatereducing bacterium Desulfovibrio africanus. Biochim Biophys Acta 1250: 49-59.
18. Pieulle L, Magro V, Hatchikian EC (1997) Isolation and analysis of the gene encoding the pyruvate-ferredoxin oxidoreductase of Desulfovibrio africanus, production of the recombinant enzyme in Escherichia coli, and effect of carboxy-terminal deletions on its stability. J Bacteriol 179: 5684-5692.
19. Pomposiello PJ, Bennik MH, Demple B (2001) Genome-wide transcriptional profiling of the Escherichia coli responses to superoxide stress and sodium salicylate. J Bacteriol 183: 3890-3902.
20. Pomposiello PJ, Demple B (2000) Identification of SoxS-regulated genes in Salmonella enterica Serovar Typhimurium. J Bacteriol 182: 23-29.
21. Pomposiello PJ, Koutsolioutsou A, Carrasco D, Demple B (2003) SoxRSregulated expression and genetic analysis of the yggX gene of Escherichia coli. J Bacteriol 185: 6624-6632.
22. Ptitsyn LR, Altman IB, Kotliarova VA, Mokhova ON, Yampolskaya TA, et al (2007) A method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family. Internetional Patent WO2008/010565 A2.
23. 2 fixation. Biochim Biophys Acta 1784: 1873-1898.
24. Reed JL, Vo TD, Schilling CH, Palsson BO (2003). An expanded genome-scale model of Escherichia coli K-12 (iJR904 GSM/GPR). Genome Biol 4: R54.
25. Sambrook J, Russell DW (2001) Molecular Cloning: A Laboratory Manual, 3rd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
26. Savrasova EA (2007) Development of mini-Mu system for highly effective integration and amplification of genetic material into chromosome of E. coli without selective marker. PhD dissertation, State Research Institute of Genetics and Selection of Industrial Microorganisms, Moscow, Russian Federation.
27. Schmidt FR (2005) Optimization and scale up of industrial fermentation processes. Appl Microbiol Biotechnol 68: 425-435.
28. Serres MH, Gopal S, Nahum LA, Liang P, Gaasterland T, et al (2001) A functional update of the Escherichia coli K-12 genome. Genome Biol 2: research0035.1-research0035.7.
29. Sprenger GA (2007) From scratch to value: engineering Escherichia coli wild type cells to the production of L-phenylalanine and other fine chemicals derived from chorismate. Appl Microbiol Biotechnol 75: 739-749.
30. Tabolina E, Rybak K, Khourges E, Voroshilova E, Gusyatiner M (2005) Method for producing L-amino acid using bacteria belonging to the genus Escherichia. US Patent 7476531.
31. Vita N, Hatchikian EC, Nouailler M, Dolla A, Pieulle L (2008) Disulfide bonddependent mechanism of protection against oxidative stress in pyruvateferredoxin oxidoreductase of anaerobic Desulfovibrio bacteria. Biochemistry 47: 957-964.
32. Zeikus JG, Fuchs G, Kenealy W, Thauer RK (1977) Oxidoreductase involved in cell carbon synthesis of Methanobacterium thermoautotrophicum. J Bacteriol 132: 604-613.