Integrative environmental genomics of cod (Gadus morhua): The proteomics approach

Journal of Toxicology and Environmental Health - Part A: Current Issues

Volumn 74, Issue 7-9, 2011, Pages 494-507

  Karlsen, Odd André ^a   Bjrneklett, Silje ^a   Berg, Karin ^a   Bratts, Marianne ^a   Bohne Kjersem, Anneli ^a   Grsvik, Bjrn Einar ^b   Goksyr, Anders ^a  

^a UNIVERSITY OF BERGEN   (Norway)

^b INSTITUTE OF MARINE RESEARCH   (Norway)

Author keywords

[No Author keywords available]

Indexed keywords

14 3 3B1 PROTEIN; 14 3 3E1 PROTEIN; 60 S ACIDIC RIBOSOMAL PROTEIN PO; ACONITASE 1; ALDEHYDE DEHYDROGENASE; ALPHA 1 ANTITRYPSIN; ALPHA 2 ANTIPLASMIN; ALPHA ACTIN; ALPHA ACTININ; ALPHA ENOLASE; ANTIQUITIN; APOLIPOPROTEIN A; DOWN SYNDROME CRI REGION GENE 2 PRODUCT; EF 1BETA2 PROTEIN; FIBRINOGEN; FRUCTOSE BISPHOSPHATE ALDOLASE; GLUTATHIONE TRANSFERASE T1; HEAT SHOCK PROTEIN 90; HEATSHOCK COGNATE 71 KD PROTEIN; HEMOPEXIN; KERATIN; METHYLMERCURY; MITOFILIN; MYOSIN; PROTEIN; PROTHROMBIN; PYROLINE 5 CARBOXYLATE DEHYDROGENASE; SEROTRANSFERRIN; TROPOMYOSIN; UNCLASSIFIED DRUG; UNINDEXED DRUG;

ANIMAL TISSUE; ATLANTIC COD; BRAIN; CONFERENCE PAPER; DATA BASE; ENVIRONMENTAL STRESS; EXPRESSED SEQUENCE TAG; FISH GENETICS; LIVER; NONHUMAN; PRIORITY JOURNAL; PROTEOMICS; TOXICOLOGICAL PARAMETERS; TRANSCRIPTOMICS;

ANIMALS; BRAIN; ENVIRONMENTAL MONITORING; EXPRESSED SEQUENCE TAGS; FISH PROTEINS; GADUS MORHUA; GENE EXPRESSION PROFILING; LIVER; METAGENOMICS; METHYLMERCURY COMPOUNDS; POLYCHLORINATED BIPHENYLS; PROTEOME; WATER POLLUTANTS, CHEMICAL;

GADUS MORHUA;

EID: 79952590867     PISSN: 15287394     EISSN: 10872620     Source Type: Journal    
DOI: 10.1080/15287394.2011.550559     Document Type: Conference Paper

References (87)

1. Aas, E., Baussant, T., Balk, L., Liewenborg, B., and Andersen, O. K. 2000. PAH metabo-lites in bile, cytochrome P4501A and DNA adducts as environmental risk parameters for chronic oil exposure: A laboratory exper-iment with Atlantic cod. Aquat. Toxicol. 51:241-258. (Pubitemid 32412104)
2. Atchinson, W D., and Hare, M. F. 1994. Mechanisms of methylmercury- induced neurotoxicity FASEBJ. 8:622-629. (Pubitemid 24198679)
3. Belletti, S., Orlandini, G., Vettori, M. V., Mutti, A., Uggeri, J., Scandroligo, R., Alinovi, R., and Gatti, R. 2002. Time course asses-ment of methylmercury effects on C6 glioma cells: Submicromolar concentrations induce oxidative DNA damage and apoptosis. J. Neurosci. Res. 70:703-711. (Pubitemid 35397144)
4. Berg, K., Puntervoll, P., Valdersnes, S., and Goksøyr, A. 2010. Responses in the brain proteome of Atlantic cod (Gadus morhua) exposed to methylmercury. Aquat. Toxicol. 100:51-65.
5. Bern, H. A. 1992. The development of the role of hormones in development\A double remembrance. Endocrinology 131: 2037-2038.
6. Blaxter, J. H. S. 1986. development of sense organs and behaviour in teleost larvae with special reference to feeding and preda-tor avoidance. Trans. Am. Fish. Soc 115, 98-114.
7. Bohne-Kjersem, A., Bache, N., Meier, S., Nyhammer, G., Roepstorff, P., Sæle, O., Goksøyr, A., and Grøsvik, B. E. 2010. Biomarker candidate discovery in Atlantic cod (Gadus morhua) continuously exposed to North Sea produced water from eggto fry. Aquat. Toxicol. 96:280-289.
8. Bohne-Kjersem, A., Skadsheim, A., Goksøyr, A., and Grøsvik, B. E. 2009. Candidate biomarker discovery in plasma of juvenile cod (Gadus morhua) exposed to crude North Sea oil, alkyl phenols and polycyclic aro-matic hydrocarbons (PAHs). Mar. Environ. Res. 68:268-277.
9. Bottazzi, B., Garlanda, C., Salvatori, G., Jeannin, P., Manfredi, A., and Mantovani, A. 2006. Pentraxins as a key component of innate immunity. Curr. Opin. Immunol. 18: 10-15. (Pubitemid 43049642)
10. Bradbury M. W. 1997. Transport of iron in the blood-brain-cerebrospinal fluid system. J. Neurochem. 69. 443-454. (Pubitemid 27327945)
11. Bucheli, T. D., and Fent, K. 1995. Induction of cytochrome-P450 as a biomarker for environmental contamination in aquatic ecosystems. Crit Rev. Environ. Sci. Technol. 25:201-268.
12. Caliani, I., Porcelloni, S., Mori, G., Frenzilli, G., Ferraro, M., Marsili, L., Casini, S., and Fossi, M. C. 2009. Genotoxic effects of produced waters in mosquito fish (Gambusia affinis). Ecotoxicology 18:75-80.
13. Cambier, S., Bernard, G., Mesmer-Dudons, N., Gonzalez, P., Rossignol, R., Brethes, D., and Bourdineaud, J. P. 2009. At environmen-tal doses, dietary methylmercury inhibits mitochondrial energy metabolism in skeletal muscles of the zebrafish (Danio rerio). Int. J. Biochem. Cell B 41:791-799.
14. Cardinali, M., Maradonna, F., Olivotto, I., Bortoluzzi, G., Mosconi, G., Polzonetti-Magni, A. M., and Carnevali, O. 2004. Temporary impairment of reproduction in freshwater teleost exposed to nonylphenol. Reprod. Toxicol. 18:597-604. (Pubitemid 38609290)
15. Castoldi, A. F., Coccini, T., Ceccatelli, S., and Manzo, L. 2001. Neurotoxicity and molecu-lar effects of methylmercury Brain Res. Bull. 55:197-203.
16. Cernichiari, E., Myers, G. J., Ballatori, N., Zareba, G., Vyas, J., and Clarkson, T. 2007. The biological monitoring of prenatal expo-sure to methylmercury. Neurotoxicology 28:1015-1022. (Pubitemid 47519795)
17. Chaitin, M. H. and Burnside, B. 1989. Actin filament polarity at the site of rod outer segment disk morphogenesis. Invest. Ophthalmol. Vis. Sci. 30:2461-2469. (Pubitemid 20021785)
18. Clarkson, T W 1997. The toxicology of mer-cury. Crit. Rev. Clin. Lab. Sci. 34:369-403.
19. Clarkson, T W 2002. The three modern faces of mercury. Environ. Health Perspect. 110(suppl. 1):11-23. (Pubitemid 34163090)
20. Colborn, T., vom Saal, F S., and Soto, A. M. 1993. Developmental effects of endocrine-disrupting chemicals in wildlife and humans. Environ. Health Perspect. 101:378-384. (Pubitemid 23343683)
21. DeRosa, C., Richter, P., Pohl, H., and Jones, D. E. 1998. environmental exposures that affect the endocrine system: Public health implications. J. Toxicol. Environ. Health B 1:3-26. (Pubitemid 128693554)
22. Dreiem, A., and Seegal, R. F 2007. Methylmercury-induced changes in mitochondrial function in striatal synap-tosomes are calcium-dependent and ROS-independent. Neurotoxicology 28:720-726. (Pubitemid 47058574)
23. Eriksson, S., and Svenson, A. 1978. Catalytic effects by thioltransferase on transfer of methylmercury and para-mercuribenzoate from macromolecules to low-molecular weight thiol compounds. Toxicology 10:115-122. (Pubitemid 8373688)
24. Evanson, M., and Van Der Kraak, G. J. 2001. Stimulatory effects of selected PAHs on testosterone production in goldfish and rain-bow trout and possible mechanisms of action. Comp. Biochem. Physiol. C Toxicol. Pharmacol. 130:249-258. (Pubitemid 32905303)
25. Falk-Petersen, I. B. 2005. Comparative organ differentiation during early life stages of marine fish. Fish Shellfish Immunol. 19: 397-412. (Pubitemid 40727975)
26. Farmen, E., Harman, C., Hylland, K., and Tollefsen, K. E. 2010. Produced water extracts from North Sea oil production plat-forms result in cellular oxidative stress in a rainbow trout in vitro bioassay Mar. Pollut. Bull. 60:1092-1098.
27. Fisher, B. E. 1999. Most unwanted persistent organic pollutants. Environ. Health Perspect. 107:A18-A23.
28. Fonnum, F., and Mariussen, E. 2009. Mechanisms involved in the neurotoxic effects of environmental toxicants such as polychlorinated biphenyls and bromi-nated flame retardants. J. Neurochem. 111:1327-1347.
29. Forne, I., Abian, J., and Cerda, J. 2010. Fish proteome analysis: Model organisms and non-sequenced species. Proteomics 10: 858-872.
30. Giesy, J. P., and Kannan, K. 1998. Dioxin-like and non-dioxin-like toxic effects of poly-chlorinated biphenyls (PCBs): Implications for risk assessment. Crit. Rev. Toxicol. 28: 511-569. (Pubitemid 28562764)
31. Goksøyr, A. 1985. Purification of hepatic microsomal cytochromes P-450 from beta-naphthoflavone-treated Atlantic cod (Gadus morhua), a marine teleost fish. Biochim. Biophys. Acta 840:409-417. (Pubitemid 15069415)
32. Goksøyr, A., Andersson, T., Buhler, D. R., Stegeman, J. J., Williams, D. E., Yusheng, Z., and Förlin, L. 1988. An immunological com-parison of microsomal B-naphthoflavone-inducible cytochrome-P-450 isozymes in different fish species. Aquat. Toxicol. 11: 432-433.
33. Goksøyr, A., Andersson, T., Hansson, T., Klungsøyr, J., Zhang, Y., and Förlin, L. 1987. Species characteristics of the hepatic xenobi-otic and steroid biotransformation systems of two teleost fish, Atlantic cod (Gadus morhua) and rainbow trout (Salmo gairdneri). Toxicol. Appl. Pharmacol. 89:347-360.
34. Goksøyr, A., Solbakken, J. E., and Klungsøyr, J. 1986. Regioselective metabolism of phenan-threne in Atlantic cod (Gadus morhua): Studies on the effects of monooxygenase inducers and role of cytochromes P-450. Chem. Biol. Interact. 60:247-263. (Pubitemid 17212869)
35. Goksøyr, A., and Solberg, T. S. 1987. Cytochromes-P-450 in fish larvae\Immunochemical detection of responses to oil pollution. SARSIA 72:405-407.
36. Görg, A., Weiss, W., and Dunn, M. J. 2004. Current two-dimensional electrophoresis technology for proteomics. Proteomics 4:3665-3685. (Pubitemid 39657447)
37. Gregus, Z. 2008. Mechanisms of toxicity. In Casarett and Doulĺs toxicology: The basic science of poisons, pp. 35-81, ed. C. D. Klasen, vol. 48. New York: McGraw-Hill Profesional.
38. Gunning, P., Weinberger, R., and Jeffrey, P. 1997. Actin and tropomyosin isoforms in morphogenesis. Anat. Embryol. 195: 311-315. (Pubitemid 27156254)
39. Hamoutene, D., Samuelson, S., Lush, L., Burt, K., Drover, D., King, T., and Lee, K. 2010. In vitro effect of produced water on cod, Gadus morhua, sperm cells and fertiliza-tion. Bull. Environ. Contam. Toxicol. 84: 559-563.
40. Hasselberg, L., Meier, S., and Svardal, A. 2004. Effects of alkylphenols on redox status in first spawning Atlantic cod (Gadus morhua). Aquat. Toxicol. 69:95-105.
41. Holth, T. F., Beylich, B. A., Skarphedinsdottir, H., Liewenborg, B., Grung, M., and Hylland, K. 2009. Genotoxicity of environmentally relevant concentrations of water-soluble oil components in cod (Gadus morhua). Environ. Sci. Technol. 43:3329-3334.
42. Jaffe, J. D., Keshishian, H., Chang, B., Addona, T. A., Gillette, M. A., and Carr, S. A. 2008. Accurate inclusion mass screening: a bridge from unbiased discovery to targeted assay development for biomarker verifica-tion. Mol. Cell. Proteom. 7:1952-1962.
43. Jonsson, H., Schiedek, D., Goksoyr, A., and Grosvik, B. E. 2006. Expression of cytoskele-tal proteins, cross-reacting with anti-CYP1A, in Mytilus sp. exposed to organic con-taminants. Aquat. Toxicol. 78(suppl. 1): S42-S48.
44. Keyvanshokooh, S., Vaziri, B., Gharaei, A., Mahboudi, F., Esmaili-Sari, A., and Shahriari-Moghadam, M. 2009. Proteome modifi-cations of juvenile beluga (Huso huso) brain as an effect of dietary methylmer-cury. Comp. Biochem. Physiol. D Genom. Proteom. 4:243-248.
45. Klaper, R., Rees, C B., Drevnick, P., Weber, D., Sandheinrich, M., and Carvan, M. J. 2006. Gene expression changes related to endocrine function and decline in reproduc-tion in fathead minnow (Pimephales prome-las) after dietary metylmercury exposure. Environ. Health Perspect. 114:1337-1343. (Pubitemid 44314754)
46. Ku, N. O., Zhou, X., Toivola, D. M., and Omary, M. B. 1999. The cytoskeleton of digestive epithelia in health and disease. Am. J. Physiol. 277:G1108-G1137. (Pubitemid 30046014)
47. Lie, K. K., Meier, S., and Olsvik, P. A. 2009. Effects of environmental relevant doses of pollutants from offshore oil production on Atlantic cod (Gadus morhua). Comp. Biochem. Physiol. C Toxicol. Pharmacol. 150:14-149.
48. Liu, Y. 2007. involvement of plasminogen acti-vator inhibitor type 1 in spermatogenesis, sperm capacitation and fertilization. Semin. Thromb. Hemostasis 33:29-40. (Pubitemid 46184028)
49. Livingstone, D. R. 2001. Contaminant-stimulated reactive oxygen species production and oxidative damage in aquatic organisms. Mar. Pollut. Bull. 42:656-656.
50. Longnecker, M. P., Wolff, M. S., Gladen, B. C., Brock, J. W., Grandjean, P., Jacobson, J. L., Korrick, S. A., Rogan, W J., Weisglas-Kuperus, N., Hertz-Picciotto, I., Ayotte, P., Stewart, P., Winneke, G., Charles, M. J., Jacobson, S. W., Dewailly, E., Boersma, E. R., Altshul, L. M., Heinzow, B., Pagano, J. J., and Jensen, A. A. 2003. Comparison of poly-chlorinated biphenyl levels across studies of human neurodevelopment. Environ. Health Perspect. 111:65-70.
51. Machala, M., Vondracek, J., Blaha, L., Ciganek, M., and Neca, J. V. 2001. Aryl hydrocar-bon receptor-mediated activity of mutagenic polycyclic aromatic hydrocarbons deter-mined using in vitro reporter gene assay. Mutat. Res. 497:49-62. (Pubitemid 32781806)
52. Mariussen, E., and Fonnum, F. 2006. Neurochemical targets and behavioral effects of organohalogen compounds: An update. Crit. Rev. Toxicol. 36:253-289.
53. Meier, S., Andersen, T. E., Norberg, B., Thorsen, A., Taranger, G. L., Kjesbu, O. S., Dale, R., Morton, H. C., Klungsøyr, J., and Svardal, A. 2007. Effects of alkylphe-nols on the reproductive system of Atlantic cod (Gadus morhua). Aquat. Toxicol. 81: 207-218. (Pubitemid 46223589)
54. Meier, S., Craig Morton, H., Nyhammer, G., Grøsvik, B. E., Makhotin, V., Geffen, A., Boitsov, S., Kvestad, K. A., Bohne-Kjersem, A., Goksøyr, A., Folkvord, A., Klungsøyr, J., and Svardal, A. 2010. Development of Atlantic cod (Gadus morhua) exposed to pro-duced water during early life stages: Effects on embryos, larvae, and juvenile fish. Mar. Environ. Res. 70:383-394.
55. Mela, M., Randi, M. A., Ventura, D. F., Carvalho, C. E., Pelletier, E., and Oliveira Ribeiro, C. A. 2007. Effects of dietary methylmercury on liver and kidney histol-ogy in the neotropical fish Hoplias mal-abaricus. Ecotoxicol. Environ. Safety 68: 426-435. (Pubitemid 47513742)
56. Miura, K., Koide, N., Himeno, S., Nakagawa, I., and Imura, N., 1999. The involvement of microtubular disruption in methylmercury-induced apoptosis in neuronal and nonneu-ronal cell lines. Toxicol. Appl. Pharmacol. 160:279-288. (Pubitemid 29536376)
57. Myhre, L. P., Bausant, T., Sundt, R., Sanni, S., Vabø, R., Skjoldal, H. R., and Klungsøyr, J. 2005. Risk assessment of reproductive effects of alkyl phenols in produced water on fish stocks in the North Sea. Vol. AM-2004/018.
58. Nakayama, K., Oshima, Y., Hiramatsu, K., Shimasaki, Y., and Honjo, T. 2005. Effects of polychlorinated biphenyls on the schooling behavior of Japanese medaka (Oryzias latipes). Environ. Toxicol. Chem. 24: 2588-2593. (Pubitemid 41445195)
59. Neff, J. M. 2002. Bioaccumulation in marine organisms. Amsterdam: Elsevier.
60. Nicolas, J. M. 1999. Vitellogeneis in fish and the effects of polycyclic aromatic hydro-carbon contaminants. Aquat. Toxicol. 45: 77-90.
61. ÓConnell, C P. 1981. Development in organ systems in the northern anchovy Engraulis mordax and other teleosts. J. Zool. 21: 429-446.
62. Perez, L. M., Milkiewicz, P., Ahmed-Choudhury, J., Elias, E., Ochoa, J. E., Sanchez Pozzi, E. J., Coleman, R., and Roma, M. G. 2006. Oxidative stress induces actin-cytoskeletal and tight-junctional alterations in hepatocytes by a Ca2+-dependent, PKC-mediated mechanism: Protective effect of PKA. Free Radical Biol. Med. 40:2005-2017. (Pubitemid 43728868)
63. Poplinskaia, V. A. 1995. The cytostructure and morpheogenesis of the rod outer segments. Ontogenes 26:5-21.
64. Rajasingh, H., Oyehaug, L., Vage, D. I., and Omholt, S. W 2006. Carotenoid dynamics in Atlantic salmon. BMC Biol. 4:10.
65. Ratcliffe, H. E., Swanson, G. M., and Fischer, L. J. 1996. human exposure to mercury: A critical assessment of the evidence of adverse effects. J. Toxicol. Environ. Health 49:221-270. (Pubitemid 26363180)
66. Reardon, A. M., and Bhat, H. K. 2007. Methylmercury neurotoxicity: Role of oxida-tive stress. Toxicol. Environ. Chem. 89: 535-554. (Pubitemid 47038234)
67. st century. Environ. Health Perspect. 118:1047-1050.
68. Sanfeliu, C., Sebastia, J., Cristofol, R., and Rodriguez-Farre, E. 2003. Neurotoxicity of organomercurial compounds. Neurotoxicol. Res. 5:283-305.
69. Santodonato, J. 1997. Review of the estrogenic and antiestrogenic activity of polycyclic aro-matic hydrocarbons: Relationship to car-cinogenicity. Chemosphere 34:835-848. (Pubitemid 27117423)
70. Schaffeld, M., and Schultess, J. 2006. Genes coding for intermediate filament proteins closely related to the hagfish "thread ker-atins (TK)" alpha and gamma also exist in lamprey, teleosts and amphibians. Exp. Cell Res. 312:1447-1462.
71. Schmidt, F., Donahoe, S., Hagens, K., Mattow, J., Schaible, U. E., Kaufmann, S. H., Aebersold, R., and Jungblut, P. R. 2004. Complementary analysis of the Myco-bacterium tuberculosis proteome by two-dimensional electrophoresis and isotope-coded affinity tag technology. Mol. Cell. Proteom. 3:24-42. (Pubitemid 38697102)
72. Schwaiger, J., Mallow, U., Ferling, H., Knoerr, S., Braunbeck, T., Kalbfus, W., and Negele, R. D. 2002. How estrogenic is nonylphe-nol? A transgenerational study using rainbow trout (Oncorhynchus mykiss) as a test organ-ism. Aquat. Toxicol. 59:177-189. (Pubitemid 34803200)
73. Schwarzenberg, S. J., Sharp, H. L., Berry, S. A., Manthei, R. D., and Seelig, S. 1987. Hormonal regulation of serum alpha 1-antitrypsin and hepatic alpha 1-antitrypsin mRNA in rats. Biochem. Biophys. Res. Commun. 147:936-941. (Pubitemid 17150577)
74. Steinbacher, P., Haslett, J. R., Six, M., Gollmann, H. P., Sanger, A. M., and Stoiber, W 2006. Phases of myogenetic cell acti-vation and possible role of dermomyotome cells in teleost muscle formation. Dev. Dyn. 235:3132-3143. (Pubitemid 44749703)
75. Stephens, S. M., Frankling, S. C., Stagg, R. M., and Brown, J. A. 2000. Sub-lethal effects of exposure of juvenile turbot to oil produced water. Mar. Pollut. Bull. 40:928-937.
76. Sturve, J., Hasselberg, L., Falth, H., Celander, M., and Förlin, L. 2006. Effects of North Sea oil and alkylphenols on biomarker responses in juvenile Atlantic cod (Gadus morhua). Aquat. Toxicol. 78(suppl. 1):S73-S78.
77. Sweet, L. I., and Zelikoff, J. T. 2001. Toxicology and immunotoxicology of mercury: A com-parative review in fish and humans. J. Toxicol. Environ. Health B 4:161-205. (Pubitemid 32397774)
78. Tam, S. P., Archer, T. K., and Deeley, R. G. 1985. Effects of estrogen on apolipoprotein secretion by the human hepatocarcinomona cell line, HepG2. J. Biol. Chem. 260: 1670-1675. (Pubitemid 15145443)
79. Terrier, B., Degand, N., Guilpain, P., Servettaz, A., Guillevin, L., and Mouthon, L 2007. Alpha-enolase: A target of antibodies in infectious and autoimmune diseases. Autoimmun. Rev. 6:176-182. (Pubitemid 46201958)
80. Tollefsen, K. E., Finne, E. F., Romstad, R., and Sandberg, C. 2006. Effluents from oil production activities contain chemicals that interfere with normal function of intra-and extra-cellular estrogen binding proteins. Mar. Environ. Res. 62(suppl.):S191-S194. (Pubitemid 43867708)
81. Ung, C. Y., Lam, S. H., Hlaing, M. M., Winata, C.L., Korzh, S., Mathavan, S.,and Gong, Z. 2010. Mercury induced hepatoxicity in zebrafish: In vivo mechanistic insights from transcriptome analysis, phenotype anchor-ing and targeted gene expression validation. BMC Genom. 11:212.
82. Van Nassauw, L., Harrison, F., and Callebaut, M. 1991. Localisation of smooth-muscle markers in the ovaries of some ectothermic vertebrates. Anat. Rec 229: 439-446.
83. Venkatesh, B., Tay, B.H., Elgar, G., and Brenner, S. 1996. Isolation, characterization and evolution of nine pufferfish (Fugu rubripes) actin genes. J. Mol. Biol. 259: 655-665. (Pubitemid 26226006)
84. Waters, M. D., and Fostel, J. M. 2004. Toxicogenomics and systems toxicology: Aims and prospects. Nat. Rev. Genet. 5: 936-948. (Pubitemid 39564256)
85. Weiss, W., Weiland, F., and Gorg, A. 2009. Protein detection and quantitation tech-nologies for gel-based proteome analysis. Methods Mol Biol. 564:59-82.
86. Williams, D. S., Arikawa, K., and Paallysaho, T. 1990. Cytoskeletal components of the adherens junctions between photoreceptors and the supportive Muller cells. J. Comp. Neurol. 295:155-164. (Pubitemid 20134824)
87. Williams, D. S., Hallett, M. A., and Arikawa, K., 1992. Association of myosin with the con-nectingcilium of rod photoreceptors.J. Cell. Sci. 103:183-190.