Dual-axis electron tomography of biological specimens: Extending the limits of specimen thickness with bright-field STEM imaging

Journal of Structural Biology

Volumn 174, Issue 1, 2011, Pages 107-114

  Sousa, Alioscka A ^a   Azari, Afrouz A ^a   Zhang, Guofeng ^a   Leapman, Richard D ^a  

^a NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING   (United States)

Author keywords

Electron tomography; Monte Carlo simulations; Scanning transmission electron microscopy; STEM tomography

Indexed keywords

GOLD NANOPARTICLE;

ARTICLE; MONTE CARLO METHOD; PANCREAS ISLET BETA CELL; PRIORITY JOURNAL; SCANNING TRANSMISSION ELECTRON MICROSCOPY; SIMULATION; TRANSMISSION ELECTRON MICROSCOPY;

ANIMALS; ELECTRON MICROSCOPE TOMOGRAPHY; INSULIN-SECRETING CELLS; MICE; MICROSCOPY, ELECTRON, SCANNING TRANSMISSION; MONTE CARLO METHOD;

EID: 79952438294     PISSN: 10478477     EISSN: 10958657     Source Type: Journal    
DOI: 10.1016/j.jsb.2010.10.017     Document Type: Article

References (27)

1. Aoyama K., Takagi T., Hirase A., Miyazawa A. STEM tomography for thick biological specimens. Ultramicroscopy 2008, 109:70-80.
2. Bárcena M., Koster A.J. Electron tomography in life science. Sem. Cell. Dev. Biol. 2009, 20:920-930.
3. Biskupek, J., Leschner, J., Walther, P., Kaiser, U., 2010. Optimization of STEM tomography acquisition - A comparison of convergent beam and parallel beam STEM tomography. Ultramicroscopy. doi:10.1016/j.ultramic.2010.05.008.
4. Bouwer J.C., MacKey M.R., Lawrence A., Deerinck T.J., Jones Y.Z., Terada M., Martone M.E., Peltier S., Ellisman M.H. Automated most-probable loss tomography of thick selectively stained biological specimens with quantitative measurement of resolution improvement. J. Struct. Biol. 2004, 148:297-306.
5. de Jonge N., Peckys D.B., Kremers G.J., Piston D.W. Electron microscopy of whole cells in liquid with nanometer resolution. Proc. Nat. Acad. Sci. U.S.A 2009, 106:2159-2164.
6. de Jonge, N., Poirier-Demers, N., Demers, H., Peckys, D.B., Drouin, D., 2010. Nanometer-resolution electron microscopy through micrometers-thick water layers. Ultramicroscopy. doi: 10.1016/j.ultramic.2010.04.001.
7. Gadelha C., Rothery S., Morphew M., McIntosh J.R., Severs N.J., Gull K. Membrane domains and flagellar pocket boundaries are influenced by the cytoskeleton in African trypanosomes. Proc. Nat. Acad. Sci. U.S.A. 2009, 106:17425-17430.
8. Gauvin R., Hovington P., Drouin D. Quantification of spherical inclusions in the scanning electron microscope using Monte Carlo simulations. Scanning 1995, 17:202-219.
9. Han K.F., Sedat J.W., Agard D.A. Practical image restoration of thick biological specimens using multiple focus levels in transmission electron microscopy. J. Struct. Biol. 1997, 120:237-244.
10. Hanssen E., Carlton P., Deed S., Klonis N., Sedat J., DeRisi J., Tilley L. Whole cell imaging reveals novel modular features of the exomembrane system of the malaria parasite, Plasmodium falciparum. Int. J. Parasit. 2010, 40:123-134.
11. Heymann J.A., Hayles M., Gestmann I., Giannuzzi L.A., Lich B., Subramaniam S. Site-specific 3D imaging of cells and tissues with a dual beam microscope. J. Struct. Biol. 2006, 155:63-73.
12. Hoenger A., McIntosh J.R. Probing the macromolecular organization of cells by electron tomography. Curr. Opin. Cell. Biol. 2009, 21:89-96.
13. Hohmann-Marriott M.F., Sousa A.A., Azari A.A., Glushakova S., Zhang G., Zimmerberg J., Leapman R.D. Nanoscale 3D cellular imaging by axial scanning transmission electron tomography. Nat. Methods 2009, 6:729-731.
14. Hovington P., Drouin D., Gauvin R. CASINO: a new Monte Carlo code in C language for electron beam interaction - Part I: Description of program. Scanning 1997, 19:1-14.
15. Hyun J.K., Ercius P., Muller D.A. Beam spreading and spatial resolution in thick organic specimens. Ultramicroscopy 2008, 109:1-7.
16. Joy, D.C., 1995. Monte Carlo Modeling for Electron Microscopy and Microanalysis, Oxford University Press, New York.
17. Kremer J.R., Mastronarde D.N., McIntosh J.R. Computer visualization of three-dimensional image data using IMOD. J. Struct. Biol. 1996, 116:71-76.
18. Leis A., Rockel B., Andrees L., Baumeister W. Visualizing cells at the nanoscale. Trends Biochem. Sci. 2009, 34:60-70.
19. Lešer V., Milani M., Tatti F., Tkalec Z.P., Štrus J., Drobne D. Focused ion beam (FIB)/scanning electron microscopy (SEM) in tissue structural research. Protoplasma 2010, 246:41-48.
20. Loos J., Sourty E., Lu K., Freitag B., Tang D., Wall D. Electron tomography on micrometer-thick specimens with nanometer resolution. Nano Lett. 2009, 9:1704-1708.
21. Martone M.E., Deerinck T.J., Yamada N., Bushong E., Ellisman M.H. Correlated 3D light and electron microscopy: Use of high voltage electron microscopy and electron tomography for imaging large biological structures. J. Histotech. 2000, 23:261-270.
22. Motoki, S., Kaneko, T., Aoyama, Y., Nishioka, H., Okura, Y., Kondo, Y., Jinnai, H., 2010. Dependence of beam broadening on detection angle in scanning transmission electron microtomography. J. Elec. Microsc. doi: 10.1093/jmicro/dfq030.
23. Noske A.B., Costin A.J., Morgan G.P., Marsh B.J. Expedited approaches to whole cell electron tomography and organelle mark-up in situ in high-pressure frozen pancreatic islets. J. Struct. Biol. 2008, 161:298-313.
24. Rocha G.M., Brandão B.A., Mortara R.A., Attias M., De Souza W., Carvalho T.M.U. The flagellar attachment zone of Trypanosoma cruzi epimastigote forms. J. Struct. Biol. 2006, 154:89-99.
25. Soto G.E., Young S.J., Martone M.E., Deerinck T.J., Lamont S., Carragher B.O., Hama K., Ellisman M.H. Serial section electron tomography: a method for three-dimensional reconstructions of large structures. NeuroImage 1994, 1:230-243.
26. Sousa A.A., Hohmann-Marriott M., Aronova M.A., Zhang G., Leapman R.D. Determination of quantitative distributions of heavy-metal stain in biological specimens by annular dark-field STEM. J. Struct. Biol. 2008, 162:14-28.
27. Sousa A.A., Hohmann-Marriott M.F., Zhang G., Leapman R.D. Monte Carlo electron-trajectory simulations in bright-field and dark-field STEM: Implications for tomography of thick biological sections. Ultramicroscopy 2009, 109:213-221.