Quantitative retrotransposon anchored PCR confirms transduction efficiency of transgenes in adult Schistosoma mansoni

Molecular and Biochemical Parasitology

Volumn 177, Issue 1, 2011, Pages 70-76

  Rinaldi, Gabriel ^a,b   Suttiprapa, Sutas ^a   Brindley, Paul J ^a  

^a GEORGE WASHINGTON UNIVERSITY MEDICAL CENTER   (United States)

^b DEPARTAMENTO DE BIOQUÍMICA   (Uruguay)

Author keywords

Anchored PCR; Integration; Luciferase; Pseudotyped retrovirus; Retrotransposon; Schistosome; Transgene

Indexed keywords

GENOMIC DNA;

ARTICLE; CHROMOSOME; CONTROLLED STUDY; FUNCTIONAL GENOMICS; GENOME; HUMAN IMMUNODEFICIENCY VIRUS 1; IN VITRO STUDY; MURINE LEUKEMIA VIRUS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROVIRUS; QUANTITATIVE ANALYSIS; REPORTER GENE; RETROPOSON; SCHISTOSOMA MANSONI; TRANSGENE; TRANSGENICS; VIRION; VIRUS DNA CELL DNA INTERACTION; VIRUS GENOME; WORM;

ANIMALS; FEMALE; MALE; POLYMERASE CHAIN REACTION; RETROELEMENTS; SCHISTOSOMA MANSONI; TRANSDUCTION, GENETIC; TRANSGENES;

SCHISTOSOMA; SCHISTOSOMA MANSONI;

EID: 79952407317     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.molbiopara.2011.01.007     Document Type: Article

References (26)

1. M. Berriman, B.J. Haas, and P.T. LoVerde The genome of the blood fluke Schistosoma mansoni Nature 460 7253 2009 352 358
2. F. Liu, Y. Zhou, and Z.Q. Wang The Schistosoma japonicum genome reveals features of host-parasite interplay Nature 460 7253 2009 345 351
3. M. Cancela, N. Ruetalo, and N. DellOca Survey of transcripts expressed by the invasive juvenile stage of the liver fluke Fasciola hepatica BMC Genomics 11 2010 p227
4. N.D. Young, R.S. Hall, A.R. Jex, C. Cantacessi, and R.B. Gasser Elucidating the transcriptome of Fasciola hepatica - a key to fundamental and biotechnological discoveries for a neglected parasite Biotechnol Adv 28 2 2009 222 231
5. J.M. Correnti, and E.J. Pearce Transgene expression in Schistosoma mansoni: introduction of RNA into schistosomula by electroporation Mol Biochem Parasitol 137 1 2004 75 79 (Pubitemid 38987944)
6. M.E. Morales, V.H. Mann, and K.J. Kines piggyBac transposon mediated transgenesis of the human blood fluke, Schistosoma mansoni FASEB J 21 13 2007 3479 3489 (Pubitemid 350075138)
7. G. Rinaldi, M.E. Morales, M. Cancela, E. Castillo, P.J. Brindley, and J.F. Tort Development of functional genomic tools in trematodes: RNA interference and luciferase reporter gene activity in Fasciola hepatica PLoS Negl Trop Dis 2 7 2008 pe260
8. C.G. Grevelding Transgenic flatworms A.G. Maule, N.J. Marks, Parasitic flatworms 2006 149 166 Oxfordshire
9. K. Brehm, and M. Spiliotis Recent advances in the in vitro cultivation and genetic manipulation of Echinococcus multilocularis metacestodes and germinal cells Exp Parasitol 119 4 2008 506 515
10. P.J. Brindley, and E.J. Pearce Genetic manipulation of schistosomes Int J Parasitol 37 5 2007 465 473 (Pubitemid 46399710)
11. M.E. Morales, G. Rinaldi, G.N. Gobert, K.J. Kines, J.F. Tort, and P.J. Brindley RNA interference of Schistosoma mansoni cathepsin D, the apical enzyme of the hemoglobin proteolysis cascade Mol Biochem Parasitol 157 2 2008 160 168
12. G. Rinaldi, M.E. Morales, and Y.N. Alrefaei RNA interference targeting leucine aminopeptidase blocks hatching of Schistosoma mansoni eggs Mol Biochem Parasitol 167 2 2009 118 126
13. M.H. Tran, T.C. Freitas, and L. Cooper Suppression of mRNAs encoding tegument tetraspanins from Schistosoma mansoni results in impaired tegument turnover PLoS Pathog 6 4 2010 pe1000840
14. T.P. Yoshino, N. Dinguirard, and MdeM. Mourao In vitro manipulation of gene expression in larval Schistosoma: a model for postgenomic approaches in Trematoda Parasitology 137 3 2010 463 483
15. K.J. Kines, V.H. Mann, and M.E. Morales Transduction of Schistosoma mansoni by vesicular stomatitis virus glycoprotein-pseudotyped Moloney murine leukemia retrovirus Exp Parasitol 112 4 2006 209 220
16. K.J. Kines, M.E. Morales, V.H. Mann, G.N. Gobert, and P.J. Brindley Integration of reporter transgenes into Schistosoma mansoni chromosomes mediated by pseudotyped murine leukemia virus FASEB J 22 8 2008 2936 2948
17. K.J. Kines, G. Rinaldi, and T.I. Okatcha Electroporation facilitates introduction of reporter transgenes and virions into schistosome eggs PLoS Negl Trop Dis 4 2 2010 pe593
18. S. Yang, P.J. Brindley, and Q. Zeng Transduction of Schistosoma japonicum schistosomules with vesicular stomatitis virus glycoprotein pseudotyped murine leukemia retrovirus and expression of reporter human telomerase reverse transcriptase in the transgenic schistosomes Mol Biochem Parasitol 174 2 2010 109 116
19. E.B. Tchoubrieva, P.C. Ong, R.N. Pike, P.J. Brindley, and B.H. Kalinna Vector-based RNA interference of cathepsin B1 in Schistosoma mansoni Cell Mol Life Sci 67 21 2010 3739 3748
20. J.H. Strauss, and E.G. Strauss Virus whose life cycle uses reverse transcriptase J.H. Strauss, E.G. Strauss, Viruses and human disease 2002 San Diego, p. 171-219
21. G.C. Langridge, M.D. Phan, and D.J. Turner Simultaneous assay of every Salmonella Typhi gene using one million transposon mutants Genome Res 19 12 2009 2308 2316
22. V.H. Mann, M.E. Morales, G. Rinaldi, and P.J. Brindley Culture for genetic manipulation of developmental stages of Schistosoma mansoni Parasitology 137 3 2010 451 462
23. T.C. Freitas, E. Jung, and E.J. Pearce TGF-beta signaling controls embryo development in the parasitic flatworm Schistosoma mansoni PLoS Pathog 3 4 2007 pe52
24. U. ODoherty, W.J. Swiggard, D. Jeyakumar, D. McGain, and M.H. Malim A sensitive, quantitative assay for human immunodeficiency virus type 1 integration J Virol 76 21 2002 10942 10950 (Pubitemid 35176211)
25. P.J. Brindley, T. Laha, D.P. McManus, and A. Loukas Mobile genetic elements colonizing the genomes of metazoan parasites Trends Parasitol 19 2 2003 79 87 (Pubitemid 36173890)
26. D.G. Ginzinger Gene quantification using real-time quantitative PCR: an emerging technology hits the mainstream Exp Hematol 30 6 2002 503 512 (Pubitemid 34626648)