RNA interference of Schistosoma mansoni cathepsin D, the apical enzyme of the hemoglobin proteolysis cascade

Molecular and Biochemical Parasitology

Volumn 157, Issue 2, 2008, Pages 160-168

  Morales, Maria E ^a   Rinaldi, Gabriel ^a,b   Gobert, Geoffrey N ^a,c   Kines, Kristine J ^a   Tort, Jose F ^b   Brindley, Paul J ^a  

^a TULANE UNIVERSITY HEALTH SCIENCES CENTER   (United States)

^b DEPARTAMENTO DE BIOQUÍMICA   (Uruguay)

^c QIMR BERGHOFER MEDICAL RESEARCH INSTITUTE   (Australia)

Author keywords

Cathepsin D; Hemoglobin proteolysis; RNAi; RT PCR; Schistosomes; Schistosomules

Indexed keywords

ASPARTIC PROTEINASE; CATHEPSIN B; CATHEPSIN D; COMPLEMENTARY DNA; CYSTEINE PROTEINASE; HEME; HEMOGLOBIN; PROTEIN KINASE R; RNA;

ARTICLE; ELECTROPORATION; ENZYME ACTIVITY; ENZYME REPRESSION; GENETIC TRANSCRIPTION; GROWTH RETARDATION; INFECTION; INTESTINE; NONHUMAN; PATHOLOGY; PHENOTYPE; PRIORITY JOURNAL; PROTEIN DEGRADATION; RNA INTERFERENCE; SCHISTOSOMA MANSONI; SCHISTOSOMULUM;

ANIMALS; CATHEPSIN B; CATHEPSIN D; ELECTROPORATION; GASTROINTESTINAL TRACT; HEME; HEMOGLOBINS; MICE; MICE, INBRED BALB C; RNA INTERFERENCE; RNA, DOUBLE-STRANDED; SCHISTOSOMA MANSONI; SURVIVAL;

MAMMALIA; MUS; SCHISTOSOMA; SCHISTOSOMA MANSONI;

EID: 38049148695     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.molbiopara.2007.10.009     Document Type: Article

References (36)

1. Brindley P.J., Kalinna B.H., Dalton J.P., et al. Proteolytic degradation of host hemoglobin by schistosomes. Mol Biochem Parasitol 1 (1997) 1-9
2. Tort J., Brindley P.J., Knox D., Wolfe K.H., and Dalton J.P. Proteinases and associated genes of parasitic helminths. Adv Parasitol (1999) 161-266
3. Wasilewski M.M., Lim K.C., Phillips J., and McKerrow J.H. Cysteine protease inhibitors block schistosome hemoglobin degradation in vitro and decrease worm burden and egg production in vivo. Mol Biochem Parasitol 2 (1996) 179-189
4. Bogitsh B.J., Kirschner K.F., and Rotmans J.P. Schistosoma japonicum: immunoinhibitory studies on hemoglobin digestion using heterologous antiserum to bovine cathepsin D. J Parasitol 3 (1992) 454-459
5. Williamson A.L., Brindley P.J., Knox D.P., Hotez P.J., and Loukas A. Digestive proteases of blood-feeding nematodes. Trends Parasitol 9 (2003) 417-423
6. Delcroix M., Sajid M., Caffrey C.R., et al. A multienzyme network functions in intestinal protein digestion by a platyhelminth parasite. J Biol Chem 51 (2006) 39316-39329
7. Dalton J.P., and Brindley P.J. Schistosome asparaginyl endopeptidase SM32 in hemoglobin digestion. Parasitol Today 3 (1996) 125
8. Brinkworth R.I., Prociv P., Loukas A., and Brindley P.J. Hemoglobin-degrading, aspartic proteases of blood-feeding parasites: substrate specificity revealed by homology models. J Biol Chem 42 (2001) 38844-38851
9. Koehler J.W., Morales M.E., Shelby B.D., and Brindley P.J. Aspartic protease activities of schistosomes cleave mammalian hemoglobins in a host-specific manner. Mem Inst Oswaldo Cruz 1 (2007) 83-85
10. Wong J.Y., Harrop S.A., Day S.R., and Brindley P.J. Schistosomes express two forms of cathepsin D. Biochim Biophys Acta 2 (1997) 156-160
11. Brindley P.J., Kalinna B.H., Wong J.Y., et al. Proteolysis of human hemoglobin by schistosome cathepsin D. Mol Biochem Parasitol 1 (2001) 103-112
12. Morales M.E., Kalinna B.H., Heyers O., et al. Genomic organization of the Schistosoma mansoni aspartic protease gene, a platyhelminth orthologue of mammalian lysosomal cathepsin D. Gene 1 (2004) 99-109
13. Krautz-Peterson G., and Skelly P.J. Schistosoma mansoni: the dicer gene and its expression. Exp Parasitol 118 (2008) 122-128
14. Skelly P.J., Da'dara A., and Harn D.A. Suppression of cathepsin B expression in Schistosoma mansoni by RNA interference. Int J Parasitol 4 (2003) 363-369
15. Krautz-Peterson G., Radwanska M., Ndegwa D., Shoemaker C.B., and Skelly P.J. Optimizing gene suppression in schistosomes using RNA interference. Mol Biochem Parasitol 2 (2007) 194-202
16. Basch P.F. Cultivation of Schistosoma mansoni in vitro. I: Establishment of cultures from cercariae and development until pairing. J Parasitol 2 (1981) 179-185
17. James E.R., and Taylor M.G. Transformation of cercariae to schistosomula: a quantitative comparison of transformation techniques and of infectivity by different injection routes of the organisms produced. J Helminthol 4 (1976) 223-233
18. Correnti J.M., Brindley P.J., and Pearce E.J. Long-term suppression of cathepsin B levels by RNA interference retards schistosome growth. Mol Biochem Parasitol 2 (2005) 209-215
19. Lazdins J.K., Stein M.J., David J.R., and Sher A. Schistosoma mansoni: rapid isolation and purification of schistosomula of different developmental stages by centrifugation on discontinuous density gradients of Percoll. Exp Parasitol 1 (1982) 39-44
20. Correnti J.M., Jung E., Freitas T.C., and Pearce E.J. Transfection of Schistosoma mansoni by electroporation and the description of a new promoter sequence for transgene expression. Int J Parasitol 37 (2007) 1107-1115
21. Dalton J.P., Day S.R., Drew A.C., and Brindley P.J. A method for the isolation of schistosome eggs and miracidia free of contaminating host tissues. Parasitology (1997) 29-32
22. Yasuda Y., Kageyama T., Akamine A., et al. Characterization of new fluorogenic substrates for the rapid and sensitive assay of cathepsin E and cathepsin D. J Biochem (Tokyo) 6 (1999) 1137-1143
23. Abramoff M.D., Magelhaes P.J., and Ram S.J. Image Processing with ImageJ. Biophoton Int 7 (2004) 36-42
24. Tzanetakis I.E., Keller K.E., and Martin R.R. The use of reverse transcriptase for efficient first- and second-strand cDNA synthesis from single- and double-stranded RNA templates. J Virol Methods 1/2 (2005) 73-77
25. McCarthy E., Stack C., Donnelly S.M., et al. Leucine aminopeptidase of the human blood flukes, Schistosoma mansoni and Schistosoma japonicum. Int J Parasitol 6 (2004) 703-714
26. Kimber M.J., McKinney S., McMaster S., Day T.A., Fleming C.C., and Maule A.G. flp gene disruption in a parasitic nematode reveals motor dysfunction and unusual neuronal sensitivity to RNA interference. FASEB J 4 (2007) 1233-1243
27. Boyle J.P., Wu X.J., Shoemaker C.B., and Yoshino T.P. Using RNA interference to manipulate endogenous gene expression in Schistosoma mansoni sporocysts. Mol Biochem Parasitol 2 (2003) 205-215
28. Knox D.P., Geldhof P., Visser A., and Britton C. RNA interference in parasitic nematodes of animals: a reality check?. Trends Parasitol 3 (2007) 105-107
29. Tavernarakis N., Wang S.L., Dorovkov M., Ryazanov A., and Driscoll M. Heritable and inducible genetic interference by double-stranded RNA encoded by transgenes. Nat Genet 2 (2000) 180-183
30. Grant B., and Hirsh D. Receptor-mediated endocytosis in the Caenorhabditis elegans oocyte. Mol Biol Cell 12 (1999) 4311-4326
31. Simmer F., Moorman C., van der Linden A.M., et al. Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals novel gene functions. PLoS Biol 1 (2003) E12
32. Hashmi S., Zhang J., Oksov Y., and Lustigman S. The Caenorhabditis elegans cathepsin Z-like cysteine protease, Ce-CPZ-1, has a multifunctional role during the worms' development. J Biol Chem 7 (2004) 6035-6045
33. Ullu E., Tschudi C., and Chakraborty T. RNA interference in protozoan parasites. Cell Microbiol 6 (2004) 509-519
34. Oliveira M.F., d'Avila J.C., Tempone A.J., et al. Inhibition of heme aggregation by chloroquine reduces Schistosoma mansoni infection. J Infect Dis 4 (2004) 843-852
35. Andrews K.T., Fairlie D.P., Madala P.K., et al. Potencies of human immunodeficiency virus protease inhibitors in vitro against Plasmodium falciparum and in vivo against murine malaria. Antimicrob Agents Chemother 2 (2006) 639-648
36. Abdulla M.H., Lim K.C., Sajid M., McKerrow J.H., and Caffrey C.R. Schistosomiasis mansoni: novel chemotherapy using a cysteine protease inhibitor. PLoS Med 1 (2007) e14