A sensitive, quantitative assay for human immunodeficiency virus type 1 integration

Journal of Virology

Volumn 76, Issue 21, 2002, Pages 10942-10950

  O'Doherty, Una ^a   Swiggard, William J ^a   Jeyakumar, Deepa ^a   McGain, David ^a   Malim, Michael H ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIRETROVIRUS AGENT; GAG PROTEIN; INTEGRASE; INTEGRASE INHIBITOR; NUCLEOSIDE;

ARTICLE; DNA EXTRACTION; FLUORESCENCE; GENE SEQUENCE; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; LATENT VIRUS INFECTION; LONG TERMINAL REPEAT; MONITORING; NONHUMAN; PERSISTENT VIRUS INFECTION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROVIRUS; QUANTITATIVE ANALYSIS; SENSITIVITY AND SPECIFICITY; T LYMPHOCYTE; VIRUS DNA CELL DNA INTERACTION;

CD4-POSITIVE T-LYMPHOCYTES; CELL LINE; DNA, VIRAL; GENE PRODUCTS, GAG; HIV-1; HUMANS; LINEAR MODELS; PROVIRUSES; SENSITIVITY AND SPECIFICITY; VIRUS INTEGRATION;

EID: 0036838605     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.76.21.10942-10950.2002     Document Type: Article

References (39)

1. Bell, P., L. J. Montaner, and G. G. Maul. 2001. Accumulation and intranuclear distribution of unintegrated human immunodeficiency virus type 1 DNA. J. Virol. 75:7683-7691.
2. Benkirane, M., P. Corbeau, V. Housset, and C. Devaux. 1993. An antibody that binds the immunoglobulin CDR3-like region of the CD4 molecule inhibits provirus transcription in HIV-infected T cells. EMBO J. 12:4909-4921.
3. Bouyac-Bertoia, M., J. D. Dvorin, R. A. M. Fouchier, Y. Jenkins, B. E. Meyer, L. I. Wu, M. Emerman, and M. H. Malim. 2001. HIV-1 infection requires a functional integrase NLS. Mol. Cell 7:1025-1035.
4. Bukrinsky, M. I., T. L. Stanwick, M. P. Dempsey, and M. Stevenson. 1991. Quiescent T lymphocytes as an inducible virus reservoir in HIV-1 infection. Science 254:423-427.
5. Butler, S. L., M. S. T. Hansen, and F. D. Bushman. 2001. A quantitative assay for HIV DNA integration in vivo. Nat. Med. 7:631-634.
6. Chun, T.-W., L. Carruth, D. Finzi, X. Shen, J. A. DiGiuseppe, H. Taylor, M. Hermankova, K. Chadwick, J. Margolick, T. C. Quinn, Y.-H. Kuo, R. Brookmeyer, M. A. Zeiger, P. Barditch-Crovo, and R. F. Siliciano. 1997. Quantification of latent tissue reservoirs and total body viral load in HIV-1 infection. Nature 387:183-188.
7. Chun, T.-W., D. Finzi, J. Margolick, K. Chadwick, D. Schwartz, and R. F. Siliciano. 1995. In vivo fate of HIV-1-infected T cells: quantitative analysis of the transition to stable latency. Nat. Med. 1:1284-1290.
8. + T cells during primary HIV-1 infection. Proc. Natl. Acad. Sci. USA 95:8869-8873.
9. Chun, T. W., and A. S. Fauci. 1999. Latent reservoirs of HIV: obstacles to the eradication of virus. Proc. Natl. Acad. Sci. USA 96:10958-10961.
10. Chun, T. W., L. Stuyver, S. B. Mizell, L. A. Ehler, J. A. Mican, M. Baseler, A. L. Lloyd, M. A. Nowak, and A. S. Fauci. 1997. Presence of an inducible HIV-1 latent reservoir during highly active antiretroviral therapy. Proc. Natl. Acad. Sci. USA 94:13193-13197.
11. Clouse, K. A., D. Powell, I. Washington, G. Poli, K. Strebel, W. Farrar, B. Barstad, J. Kovacs, A. S. Fauci, and T. M. Folks. 1989. Monokine regulation of HIV-1 expression in a chronically infected human T-cell clone. J. Immunol. 142:431-438.
12. - human immunodeficiency virus type 1 particle which are restricted for the preretrotranscription steps. J. Virol. 69:2068-2074.
13. Crotty, P. L., R. A. Staggs, P. T. Porter, A. A. Killeen, and R. C. McGlennen. 1994. Quantitative analysis in molecular diagnostics. Hum. Pathol. 25:572-579.
14. Deininger, P. L., and M. A. Batzer. 1999. Alu repeats and human disease. Mol. Genet. Metab. 67:183-193.
15. Dyrhol-Riise, A. M., P. Voltersvik, O. G. Berg, J. Olofsson, S. Kleivbo, and B. Asjo. 2001. Residual human immunodeficiency virus type 1 infection in lymphoid tissue during highly active antiretroviral therapy. AIDS Res. Hum. Retrovir. 17:577-586.
16. Englund, G., T. S. Theodore, E. O. Freed, A. Engelman, and M. A. Martin. 1995. Integration is required for productive infection of monocyte-derived macrophages by human immunodeficiency virus type 1. J. Virol. 69:3216-3219.
17. Finzi, D., M. Hermankova, T. Pierson, L. M. Carruth, C. Buck, R. E. Chaisson, T. C. Quinn, K. Chadwick, J. Margolick, R. Brookmeyer, J. Gallant, M. Markowitz, D. D. Ho, D. D. Richman, and R. F. Siliciano. 1997. Identification of a reservoir for HIV-1 in patients on highly active antiretroviral therapy. Science 278:1295-1300.
18. Folks, T., J. Justement, A. Kinter, C. A. Dinarello, and A. S. Fauci. 1987. Cytokine induced expression of HIV-1 in a chronically infected promonocytic cell line. Science 238:800-802.
19. - cell surviving infection with the acquired immune deficiency syndrome retrovirus. J. Exp. Med. 164:280-290.
20. Fouchier, R. A. M., B. E. Meyer, J. H. M. Simon, U. Fischer, and M. H. Malim. 1997. HIV-1 infection of non-dividing cells: evidence that the aminoterminal basic region of the viral matrix protein is important for Gag processing but not for post-entry nuclear import. EMBO J. 16:4531-4539.
21. Hazuda, D. J., P. Felock, M. Witmer, A. Wolfe, K. Stilmock, J. A. Grobler, A. Espeseth, L. Gabryelski, W. Schleif, C. Blau, and M. D. Miller. 2000. Inhibitors of strand transfer that prevent integration and inhibit HIV-1 replication in cells. Science 287:646-650.
22. Jelinek, W. R., and C. W. Schmid. 1983. Repetitive sequences in eukaryotic DNA and their expression. Annu. Rev. Biochem. 51:813-844.
23. 0 lymphocytes. J. Virol. 73:6526-6532.
24. 1b phase of the cell cycle is required for completion of human immunodeficiency virus type I reverse transcription in T cells. J. Virol. 72:3161-3168.
25. Malim, M. H., and B. R. Cullen. 1993. Rev and the fate of pre-mRNA in the nucleus: implications for the regulation of RNA processing in eukaryotes. Mol. Cell. Biol. 13:6180-6189.
26. Mighell, A. J., A. F. Markham, and P. A. Robinson. 1997. Alu sequences. FEBS Lett. 417:1-5.
27. Nara, P. L., W. C. Hatch, N. M. Dunlop, W. G. Robey, L. O. Arthur, M. A. Gonda, and P. J. Fischinger. 1987. Simple, rapid quantitative, syncytiumforming microassay for the detection of human immunodeficiency virus neutralizing antibody. AIDS Res. Hum. Retrovir. 3:283-302.
28. O'Doherty, U., W. J. Swiggard, and M. H. Malim. 2000. Human immunodeficiency virus type 1 spinoculation enhances infection through virus binding. J. Virol. 74:10074-10080.
29. + T cells are infected in human immunodeficiency type 1-infected individuals. J. Virol. 73:6430-6435.
30. Pauza, C. D., J. E. Galindo, and D. D. Richman. 1990. Reinfection results in accumulation of unintegrated viral DNA in cytopathic and persistent human immunodeficiency virus type 1 infection of CEM cells. J. Exp. Med. 172:1035-1042.
31. Pierson, T., J. McArthur, and R. F. Siliciano. 2000. Reservoirs for HIV-1: mechanisms for viral persistence in the presence of antiviral immune responses and antiretroviral therapy. Annu. Rev. Immunol. 18:665-708.
32. Raeymaekers, L. 2000. Basic principles of quantitative PCR. Mol. Biotechnol. 15:115-122.
33. Schrager, L. K., and M. P. D'Souza. 1998. Cellular and anatomic reservoirs of HIV-1 in patients receiving potent antiretroviral combination therapy. JAMA 280:67-71.
34. Sonza, S., A. Maerz, N. Deacon, J. Meanger, J. Mills, and S. Crowe. 1996. Human immunodeficiency virus type 1 replication is blocked prior to reverse transcription and integration in freshly isolated peripheral blood monocytes. J. Virol. 70:3863-3869.
35. Spina, C. A., J. C. Guatelli, and D. D. Richman. 1995. Establishment of a stable, inducible form of human immunodeficiency virus type 1 DNA in quiescent CD4 lymphocytes in vitro. J. Virol. 69:2877-2988.
36. Tyagi, S., and F. R. Kramer. 1996. Molecular beacons: probes that fluoresce upon hybridization. Nat. Biotechnol. 14:303-308.
37. Vandegraaff, N., R. Kumar, C. J. Burrell, and P. Li. 2001. Kinetics of human immunodeficiency virus type 1 (HIV-1) DNA integration in acutely infected cells as determined using a novel assay for detection of integrated HIV DNA. J. Virol. 75:11253-11260.
38. Zack, J. A., S. J. Arrigo, S. R. Weitsman, A. S. Go, A. Haislip, and I. S. Y. Chen. 1990. HIV-1 entry into quiescent primary lymphocytes: molecular analysis reveals a labile, latent viral structure. Cell 61:213-222.
39. Zack, J. A., A. M. Haislip, P. Krogstad, and I. S. Y. Chen. 1992. Incompletely reverse-transcribed human immunodeficiency virus type 1 genomes in quiescent cells can function as intermediates in the retroviral life cycle. J. Virol. 66:1717-1725.