First characterization of fish P-glycoprotein (abcb1) substrate specificity using determinations of its ATPase activity and calcein-AM assay with PLHC-1/dox cell line

Aquatic Toxicology

Volumn 103, Issue 1-2, 2011, Pages 53-62

  Zaja, Roko ^a   Lončar, Jovica ^a   Popovic, Marta ^a   Smital, Tvrtko ^a  

^a RUDER BO KOVI INSTITUTE   (Croatia)

Author keywords

ATPase assay; Calcein AM assay; Fish P glycoprotein (P gp); PLHC 1 cells; Substrate specificity

Indexed keywords

ADENOSINE TRIPHOSPHATASE; CALCEIN; CYCLOSPORIN; DIMPYLATE; ENDOSULFAN; MULTIDRUG RESISTANCE PROTEIN 1; NICARDIPINE; VALSPODAR; VERAPAMIL;

ECOTOXICOLOGY; FISH; MEMBRANE; PHENOTYPE; PROTEIN; SUBSTRATE;

ABCB1 GENE; ANIMAL CELL; ARTICLE; CELL CULTURE; CELL ISOLATION; CONTROLLED STUDY; CORRELATIONAL STUDY; ECOTOXICOLOGY; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME KINETICS; ENZYME SPECIFICITY; FIBROBLAST; FISH; FLUORESCENCE ANALYSIS; GENE; GENE OVEREXPRESSION; GENETIC TRANSCRIPTION; HUMAN; HUMAN CELL; HUMAN VERSUS ANIMAL COMPARISON; MEMBRANE VESICLE; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; QUANTITATIVE STUDY; WILD TYPE;

ADENOSINE TRIPHOSPHATASES; ANIMALS; BIOLOGICAL ASSAY; CELL LINE; FISHES; FLUORESCEINS; P-GLYCOPROTEIN; SUBSTRATE SPECIFICITY; WATER POLLUTANTS, CHEMICAL;

MAMMALIA;

EID: 79952358750     PISSN: 0166445X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.aquatox.2011.02.005     Document Type: Article

References (37)

1. Allen J.D., van Loevezijn A., Lakhai J.M., van der Valk M., van Tellingen O., Reid G., Schellens J.H.M., Koomen G.J., Schinkel A.H. Potent and specific inhibition of the breast cancer resistance protein multidrug transporter in vitro and in mouse intestine by a novel analogue of Fumitremorgin C. Mol. Cancer Ther. 2002, 1:417-425.
2. Aller S.G., Yu J., Ward A., Weng Y., Chittaboina S., Zhuo R., Harrell P.M., Trinh Y.T., Zhang Q., Urbatsch I.L., Chang G. Structure of P-glycoprotein reveals a molecular basis for poly-specific drug binding. Science 2009, 323:1718-1722.
3. Aurade R.M., Jayalakshmi S.K., Sreeramulu K. P-glycoprotein ATPase from the resistant pest. Helicoverpa armigera: Purification, characterization and effect of various insecticides on its transport function. Biochim. Biophys. Acta - Biomembranes 2010, 1798:1135-1143.
4. Calcagno A.M., Fostel J.M., To K.K.W., Salcido C.D., Martin S.E., Chewning K.J., Wu C.-P., Varticovski L., Bates S.E., Caplen N.J., Ambudkar S.V. Single-step doxorubicin-selected cancer cells overexpress the ABCG2 drug transporter through epigenetic changes. Br. J. Cancer 2008, 98:1515-2152.
5. Caminada D., Zaja R., Smital T., Fent K. Human pharmaceuticals modulate P-gp1 (ABCB1) transport activity in the fish cell line PLHC-1. Aquat. Toxicol. 2008, 90:214-222.
6. Cornwell M.M., Safa A.R., Felsted R.L., Gottesman M.M., Pastan I. Membrane vesicles from multidrug-resistant human cancer cells contain a specific 150- to 170-kDa protein detected by photoaffinity labeling. Proc. Natl. Acad. Sci. USA 1986, 83:3847-3850.
7. Doige C.A., Sharom F.J. Transport properties of P-glycoprotein in plasma membrane vesicles from multidrug-resistant Chinese hamster ovary cells. Biochim. Biophys. Acta 1992, 1109:161-171.
8. Epel D. Use of multidrug transporters as first line of defense against toxins in aquatic organisms. Comp. Biochem. Physiol. A-Mol. Integr. Physiol. 2003, 134:233-246.
9. Epel D., Luckenbach T., Stevenson C.N., Macmanus-Spencer L.A., Hamdoun A., Smital T. Efflux transporters: newly appreciated roles in protection against pollutants. Environ. Sci. Technol. 2008, 42:3914-3920.
10. Fent K. Fish cell lines as versatile tools in ecotoxicology: assessment of cytotoxicity, cytochrome P4501A induction potential and estrogenic activity of chemicals and environmental samples. Toxicol. In Vitro 2001, 15:477-488.
11. Haimeur A., Conseil G., Deeley R.G., Cole S.P.C. The MRP-related and BCRP/ABCG2 multidrug resistance proteins: biology, substrate specificity and regulation. Curr. Drug Metabol. 2004, 5:21-53.
12. Hegedus C., Szakacs G., Homolya L., Orban T.I., Telbisz A., Jani M., Sarkadi B. Ins and outs of the ABCG2 multidrug transporter: An update on in vitro functional assays. Adv. Drug Deliv. Rev. 2009, 61:47-56.
13. Henkel R.D., VandeBerg J.L., Walsh R.A. A microassay for ATPase. Anal. Biochem. 1988, 169:312-318.
14. Hightower L.E., Renfro J.L. Recent applications of fish cell culture to biomedical research. J. Exp. Zool. 1988, 293:349-359.
15. Holló Z., Homolya L., Hegedüs T., Sarkadi B. Transport properties of multidrug resistance-associated protein (MRP) in human tumour cells. FEBS Lett. 1996, 383:99-104.
16. Krishnamurthy P., Schuetz J.D. Role of ABCG2/BCRP in biology and medicine. Annu. Rev. Pharmacol. 2006, 46:381-410.
17. Kurelec B. The multixenobiotic resistance mechanisms in aquatic organisms. Crit. Rev. Toxicol. 1992, 22:23-43.
18. Lecoeur S., Videmann B., Mazallon M. Effect of organophosphate pesticide diazinon on expression and activity of intestinal P-glycoprotein. Toxicol. Lett. 2006, 161:200-209.
19. Litman T., Druley T.E., Stein W.D., Bates S.E. From MDR to MXR: new understanding of multidrug resistance systems, their properties and clinical significance. Cell. Mol. Life Sci. 2001, 58:931-959.
20. Lončar J., Popović M., Zaja R., Smital T. Gene expression analysis of the ABC efflux transporters in rainbow trout (Oncorhynchus mykiss). Comp. Biochem. Physiol. C 2010, 151:209-215.
21. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. Protein measurement with folin phenol reagent. J. Biol. Chem. 1951, 193:265-275.
22. Muller P.Y., Janovjak H., Miserez A.R., Dobbie Z. Processing of gene expression data generated by quantitative real-time RT-PCR. BioTechniques 2002, 32(6):1372-1379.
23. Müller M., Bakos E, Welker E., Váradi A., Germann U.A., Gottesman M.M., Morse B.S., Roninson I.B., Sarkadi B. Altered drug-stimulated ATPase activity in mutants of the human multidrug resistance protein. J. Biol. Chem. 1996, 271:1877-1883.
24. Murray K.E., Thomas S.M., Bodour A.A. Prioritizing research for trace pollutants and emerging contaminants in the freshwater environment. Environ. Pollut. 2010, 158:3462-3471.
25. Nervi P., Li-Blatter X., Äänismaa P., Seelig A. P-glycoprotein substrate transport assessed by comparing cellular and vesicular ATPase activity. Biochem. Biophys. Acta-Biomembr. 2010, 1798:515-525.
26. Pivčević B., Zaja R. Pesticides and their binary combinations as P-glycoprotein inhibitors in NIH 3T3/MDR1 cells. Environ. Toxicol. Pharmacol. 2006, 22:268-276.
27. Richter O., Glavinas H., Krajcsi P., Liehner S., Siewert B., Zech K. A novel screening strategy to identify ABCB1 substrates and inhibitors. Naunyn Schmiedebergs Arch. Pharmacol. 2008, 379:11-26.
28. Sarkadi B., Price E.M., Boucher R.C., Germann U.A., Scarborough G.A. Expression of the human multidrug resistance cDNA in insect cells generates a high activity drug-stimulated membrane ATPase. J. Biol. Chem. 1992, 267:4854-4858.
29. Simon P. Q-Gene: processing quantitative real-time RT-PCR data. Bioinformatics 2003, 19(11):1439-1440.
30. Sreeramulu K., Liu R., Sharom F.J. Interaction of insecticides with mammalian P-glycoprotein and their effect on its transport function. Biochim. Biophys. Acta-Biomembr. 2007, 1768:1750-1757.
31. Srivastava S.K., Watkins S.C., Schuetz E., Singh S.V. Role of glutathione conjugate efflux in cellular protection against benzo[a]pyrene-7,8-diol-9,10-epoxide-induced DNA damage. Mol. Carcinogen. 2002, 33:156-162.
32. Sturm A., Ziemann C., Hirsch-Ernst K.I., Segner H. Expression and functional activity of P-glycoprotein in cultured hepatocytes from Oncorhynchus mykiss. Am. J. Physiol. Regul. Integr. Comp. Physiol. 2001, 281:R1119-R1126.
33. Szakács G., Paterson J.K., Ludwig J.A., Booth-Genthe C., Gottesman M.M. Targeting multidrug resistance in cancer. Nat. Rev. Drug Discov. 2006, 5:219-234.
34. Vlaming M.L.H., Lagas J.S., Schinkel A.H. Physiological and pharmacological roles of ABCG2 (BCRP): recent findings in Abcg2 knockout mice. Adv. Drug Deliv. Rev. 2009, 61:14-25.
35. Zaja R., Klobucar R.S., Smital T. Detection and functional characterization of Pgp1 (ABCB1) and MRP3 (ABCC3) efflux transporters in the PLHC-1 fish hepatoma cell line. Aquat. Toxicol. 2007, 81:365-376.
36. Zaja R., Munic V., Klobucar R.S., Ambriovic-Ristov A., Smital T. Cloning and molecular characterization of apical efflux transporters (ABCB1 ABCB11 and ABCC2) in rainbow trout (Oncorhynchus mykiss) hepatocytes. Aquat. Toxicol. 2008, 90:322-332.
37. Zaja R., Caminada D., Loncar J., Fent K., Smital T. Development and characterization of P-glycoprotein 1 (Pgp1 ABCB1)-mediated doxorubicin-resistant PLHC-1 hepatoma fish cell line. Toxicol. Appl. Pharmacol. 2008, 227:207-218.