Use of a Dictyostelium model for isolation of genetic loci associated with phagocytosis and virulence in Klebsiella pneumoniae

Infection and Immunity

Volumn 79, Issue 3, 2011, Pages 997-1006

  Pan, Yi Jiun ^a   Lin, Tzu Lung ^a   Hsu, Chun Ru ^a   Wang, Jin Town ^a,b  

^a NATIONAL TAIWAN UNIVERSITY   (Taiwan)

^b NATIONAL TAIWAN UNIVERSITY HOSPITAL   (Taiwan)

Author keywords

[No Author keywords available]

Indexed keywords

CARRIER PROTEIN; O ANTIGEN TRANSPORTER; PROTEINASE; UNCLASSIFIED DRUG;

ARTICLE; DICTYOSTELIUM; GENE LOCUS; GENE MUTATION; KLEBSIELLA PNEUMONIAE; NONHUMAN; PHAGOCYTOSIS; PRIORITY JOURNAL;

ANIMALS; ATP-BINDING CASSETTE TRANSPORTERS; BACTERIAL PROTEINS; DICTYOSTELIUM; DNA TRANSPOSABLE ELEMENTS; ENDOPEPTIDASE CLP; FEMALE; GENETIC LOCI; HUMANS; IMMUNOBLOTTING; KLEBSIELLA PNEUMONIAE; MICE; MICE, INBRED BALB C; MICROARRAY ANALYSIS; MICROSCOPY, CONFOCAL; PHAGOCYTOSIS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; VIRULENCE;

EID: 79952304059     PISSN: 00199567     EISSN: 10985522     Source Type: Journal    
DOI: 10.1128/IAI.00906-10     Document Type: Article

References (52)

1. Abbot, S. L. 2003. Klebsiella, Enterobacter, Citrobacter, Serratia, Plesiomonas, and other Enterobacteriaceae, p. 684-700. In P. R. Murray, E. J. Baron, J. H. Jorgensen, M. A. Pfaller, and R. H. Yolken (ed.), Manual of clinical microbiology, 8th ed. American Society for Microbiology, Washington, DC.
2. Alibaud, L., et al. 2008. Pseudomonas aeruginosa virulence genes identified in a Dictyostelium host model. Cell. Microbiol. 10:729-740.
3. Applied Biosystems. 2001. User bulletin. Relative quantitation of gene expression. Applied Biosystems, Foster City, CA.
4. Benghezal, M., et al. 2006. Specific host genes required for the killing of Klebsiella bacteria by phagocytes. Cell. Microbiol. 8:139-148.
5. Blom, A. M., T. Hallstrom, and K. Riesbeck. 2009. Complement evasion strategies of pathogens - acquisition of inhibitors and beyond. Mol. Immunol. 46:2808-2817.
6. Chou, H. C., et al. 2004. Isolation of a chromosomal region of Klebsiella pneumoniae associated with allantoin metabolism and liver infection. Infect. Immun. 72:3783-3792.
7. Chuang, Y. P., C. T. Fang, S. Y. Lai, S. C. Chang, and J. T. Wang. 2006. Genetic determinants of capsular serotype K1 of Klebsiella pneumoniae causing primary pyogenic liver abscess. J. Infect. Dis. 193:645-654.
8. Chun, K. T., H. J. Edenberg, M. R. Kelley, and M. G. Goebl. 1997. Rapid amplification of uncharacterized transposon-tagged DNA sequences from genomic DNA. Yeast 13:233-240.
9. Chung, D. R., et al. 2007. Emerging invasive liver abscess caused by K1 serotype Klebsiella pneumoniae in Korea. J. Infect. 54:578-583. (Pubitemid 46731226)
10. Cornillon, S., R. A. Olie, and P. Golstein. 1998. An insertional mutagenesis approach to Dictyostelium cell death. Cell Death Differ. 5:416-425. (Pubitemid 28356818)
11. Cosson, P., et al. 2002. Pseudomonas aeruginosa virulence analyzed in a Dictyostelium discoideum host system. J. Bacteriol. 184:3027-3033. (Pubitemid 34517826)
12. Cuthbertson, L., M. S. Kimber, and C. Whitfield. 2007. Substrate binding by a bacterial ABC transporter involved in polysaccharide export. Proc. Natl. Acad. Sci. U. S. A. 104:19529-19534.
13. Cvrckova, F., F. Rivero, and B. Bavlnka. 2004. Evolutionarily conserved modules in actin nucleation: lessons from Dictyostelium discoideum and plants. Review article. Protoplasma 224:15-31. (Pubitemid 39403882)
14. Fang, C. T., Y. P. Chuang, C. T. Shun, S. C. Chang, and J. T. Wang. 2004. A novel virulence gene in Klebsiella pneumoniae strains causing primary liver abscess and septic metastatic complications. J. Exp. Med. 199:697-705.
15. Flynn, J. M., S. B. Neher, Y. I. Kim, R. T. Sauer, and T. A. Baker. 2003. Proteomic discovery of cellular substrates of the ClpXP protease reveals five classes of ClpX-recognition signals. Mol. Cell 11:671-683. (Pubitemid 36388762)
16. Foster, T. J. 2005. Immune evasion by staphylococci. Nat. Rev. Microbiol. 3:948-958.
17. Frees, D., and H. Ingmer. 1999. ClpP participates in the degradation of misfolded protein in Lactococcus lactis. Mol. Microbiol. 31:79-87.
18. Gaillot, O., E. Pellegrini, S. Bregenholt, S. Nair, and P. Berche. 2000. The ClpP serine protease is essential for the intracellular parasitism and virulence of Listeria monocytogenes. Mol. Microbiol. 35:1286-1294.
19. Gerth, U., E. Kruger, I. Derre, T. Msadek, and M. Hecker. 1998. Stress induction of the Bacillus subtilis clpP gene encoding a homologue of the proteolytic component of the Clp protease and the involvement of ClpP and ClpX in stress tolerance. Mol. Microbiol. 28:787-802.
20. Hagedorn, M., K. H. Rohde, D. G. Russell, and T. Soldati. 2009. Infection by tubercular mycobacteria is spread by nonlytic ejection from their amoeba hosts. Science 323:1729-1733.
21. Hensel, M., et al. 1995. Simultaneous identification of bacterial virulence genes by negative selection. Science 269:400-403.
22. Hilbi, H., S. S. Weber, C. Ragaz, Y. Nyfeler, and S. Urwyler. 2007. Environmental predators as models for bacterial pathogenesis. Environ. Microbiol. 9:563-575.
23. Hsieh, P. F., H. H. Lin, T. L. Lin, and J. T. Wang. 2010. CadC regulates cad and tdc operons in response to gastrointestinal stresses and enhances intestinal colonization of Klebsiella pneumoniae. J. Infect. Dis. 202:52-64.
24. Hsieh, P. F., T. L. Lin, C. Z. Lee, S. F. Tsai, and J. T. Wang. 2008. Serum-induced iron-acquisition systems and TonB contribute to virulence in Klebsiella pneumoniae causing primary pyogenic liver abscess. J. Infect. Dis. 197:1717-1727.
25. Jules, M., and C. Buchrieser. 2007. Legionella pneumophila adaptation to intracellular life and the host response: clues from genomics and transcriptomics. FEBS Lett. 581:2829-2838. (Pubitemid 46874392)
26. Ko, W. C., et al. 2002. Community-acquired Klebsiella pneumoniae bacteremia: global differences in clinical patterns. Emerg. Infect. Dis. 8:160-166.
27. Kwon, H. Y., et al. 2004. The ClpP protease of Streptococcus pneumoniae modulates virulence gene expression and protects against fatal pneumococcal challenge. Infect. Immun. 72:5646-5653.
28. Li, Z., et al. 2009. The amoebal MAP kinase response to Legionella pneumophila is regulated by DupA. Cell Host Microbe 6:253-267.
29. Link, A. J., D. Phillips, and G. M. Church. 1997. Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: application to open reading frame characterization. J. Bacteriol. 179:6228-6237.
30. Loughlin, M. F., V. Arandhara, C. Okolie, T. G. Aldsworth, and P. J. Jenks. 2009. Helicobacter pylori mutants defective in the clpP ATP-dependant protease and the chaperone clpA display reduced macrophage and murine survival. Microb. Pathog. 46:53-57.
31. Lugo, J. Z., et al. 2007. Lipopolysaccharide O-antigen promotes persistent murine bacteremia. Shock 27:186-191. (Pubitemid 46105836)
32. Ma, L. C., C. T. Fang, C. Z. Lee, C. T. Shun, and J. T. Wang. 2005. Genomic heterogeneity in Klebsiella pneumoniae strains is associated with primary pyogenic liver abscess and metastatic infection. J. Infect. Dis. 192:117-128.
33. Maniak, M., R. Rauchenberger, R. Albrecht, J. Murphy, and G. Gerisch. 1995. Coronin involved in phagocytosis: dynamics of particle-induced relocalization visualized by a green fluorescent protein tag. Cell 83:915-924. (Pubitemid 26006533)
34. Merino, S., et al. 2000. Cloning and sequencing of the Klebsiella pneumoniae O5 wb gene cluster and its role in pathogenesis. Infect. Immun. 68:2435-2440.
35. Noegel, A. A., and M. Schleicher. 2000. The actin cytoskeleton of Dictyostelium: a story told by mutants. J. Cell Sci. 113(Pt 5):759-766. (Pubitemid 30168645)
36. Ohmori, S., et al. 2002. Septic endophthalmitis and meningitis associated with Klebsiella pneumoniae liver abscess. Hepatol. Res. 22:307-312.
37. Okano, H., et al. 2002. Clinicopathological analysis of liver abscess in Japan. Int. J. Mol. Med. 10:627-630.
38. Pan, Y. J., et al. 2008. Capsular polysaccharide synthesis regions in Klebsiella pneumoniae serotype K57 and a new capsular serotype. J. Clin. Microbiol. 46:2231-2240.
39. Pederson, K. J., S. Carlson, and D. E. Pierson. 1997. The ClpP protein, a subunit of the Clp protease, modulates ail gene expression in Yersinia enterocolitica. Mol. Microbiol. 26:99-107.
40. Podschun, R., and U. Ullmann. 1998. Klebsiella spp. as nosocomial pathogens: epidemiology, taxonomy, typing methods, and pathogenicity factors. Clin. Microbiol. Rev. 11:589-603.
41. Pozos, T. C., and L. Ramakrishnan. 2004. New models for the study of Mycobacterium-host interactions. Curr. Opin. Immunol. 16:499-505.
42. Pukatzki, S., R. H. Kessin, and J. J. Mekalanos. 2002. The human pathogen Pseudomonas aeruginosa utilizes conserved virulence pathways to infect the social amoeba Dictyostelium discoideum. Proc. Natl. Acad. Sci. U. S. A. 99:3159-3164.
43. Pukatzki, S., A. T. Ma, A. T. Revel, D. Sturtevant, and J. J. Mekalanos. 2007. Type VI secretion system translocates a phage tail spike-like protein into target cells where it cross-links actin. Proc. Natl. Acad. Sci. U. S. A. 104: 15508-15513.
44. Pukatzki, S., et al. 2006. Identification of a conserved bacterial protein secretion system in Vibrio cholerae using the Dictyostelium host model system. Proc. Natl. Acad. Sci. U. S. A. 103:1528-1533.
45. Rahimian, J., T. Wilson, V. Oram, and R. S. Holzman. 2004. Pyogenic liver abscess: recent trends in etiology and mortality. Clin. Infect. Dis. 39:1654-1659.
46. Robertson, G. T., W. L. Ng, J. Foley, R. Gilmour, and M. E. Winkler. 2002. Global transcriptional analysis of clpP mutations of type 2 Streptococcus pneumoniae and their effects on physiology and virulence. J. Bacteriol. 184:3508-3520.
47. Shin, Y., et al. 2009. Single-molecule denaturation and degradation of proteins by the AAA+ ClpXP protease. Proc. Natl. Acad. Sci. U. S. A. 106: 19340-19345.
48. Solomon, J. M., A. Rupper, J. A. Cardelli, and R. R. Isberg. 2000. Intracellular growth of Legionella pneumophila in Dictyostelium discoideum, a system for genetic analysis of host-pathogen interactions. Infect. Immun. 68:2939-2947.
49. Urban, C. F., S. Lourido, and A. Zychlinsky. 2006. How do microbes evade neutrophil killing? Cell. Microbiol. 8:1687-1696. (Pubitemid 44530662)
50. Vlahou, G., et al. 2009. Yersinia outer protein YopE affects the actin cytoskeleton in Dictyostelium discoideum through targeting of multiple Rho family GTPases. BMC Microbiol. 9:138.
51. Wang, J. H., et al. 1998. Primary liver abscess due to Klebsiella pneumoniae in Taiwan. Clin. Infect. Dis. 26:1434-1438.
52. Yamamoto, T., et al. 2001. Disruption of the genes for ClpXP protease in Salmonella enterica serovar Typhimurium results in persistent infection in mice, and development of persistence requires endogenous gamma interferon and tumor necrosis factor alpha. Infect. Immun. 69:3164-3174.