PINCH1 is transcriptional regulator in podocytes that interacts with WT1 and represses podocalyxin expression

PLoS ONE

Volumn 6, Issue 2, 2011, Pages

  Wang, Dan ^a   Li, Yingjian ^a   Wu, Chuanyue ^a   Liu, Youhua ^a  

^a UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADAPTOR PROTEIN; LUCIFERASE; PARTICULARLY INTERESTING NEW CYSTEINE HISTIDINE RICH PROTEIN 1; PODOCALYXIN; TRANSFORMING GROWTH FACTOR BETA1; UNCLASSIFIED DRUG; WT1 PROTEIN; DNA BINDING PROTEIN; LIMS1 PROTEIN, HUMAN; REPRESSOR PROTEIN; SIALOGLYCOPROTEIN;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; DOWN REGULATION; ENZYME ASSAY; GENE DELETION; GENE EXPRESSION REGULATION; HUMAN; HUMAN CELL; IMMUNOPRECIPITATION; NUCLEAR EXPORT SIGNAL; NUCLEAR LOCALIZATION SIGNAL; PODOCYTE; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; SITE DIRECTED MUTAGENESIS; TISSUE SPECIFICITY; TRANSCRIPTION REGULATION; UPREGULATION; AMINO ACID SEQUENCE; CELL LINE; CHEMISTRY; GENETIC TRANSCRIPTION; GENETICS; METABOLISM; MOLECULAR GENETICS; PHYSIOLOGY; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN DOMAIN; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; CELL LINE; DNA-BINDING PROTEINS; DOWN-REGULATION; GENE EXPRESSION REGULATION; HUMANS; MOLECULAR SEQUENCE DATA; PODOCYTES; PROTEIN BINDING; PROTEIN INTERACTION DOMAINS AND MOTIFS; PROTEIN INTERACTION MAPPING; REPRESSOR PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SIALOGLYCOPROTEINS; TRANSCRIPTION, GENETIC; WT1 PROTEINS;

EID: 79952080210     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0017048     Document Type: Article

References (42)

1. Pavenstadt H, Kriz W, Kretzler M, (2003) Cell biology of the glomerular podocyte. Physiol Rev 83: 253-307.
2. Patrakka J, Tryggvason K, (2009) New insights into the role of podocytes in proteinuria. Nat Rev Nephrol 5: 463-468.
3. Li Y, Kang YS, Dai C, Kiss LP, Wen X, et al. (2008) Epithelial-to-mesenchymal transition is a potential pathway leading to podocyte dysfunction and proteinuria. Am J Pathol 172: 299-308.
4. Wiggins RC, (2007) The spectrum of podocytopathies: a unifying view of glomerular diseases. Kidney Int 71: 1205-1214.
5. Shankland SJ, (2006) The podocyte's response to injury: role in proteinuria and glomerulosclerosis. Kidney Int 69: 2131-2147.
6. Liu Y, (2010) New insights into epithelial-mesenchymal transition in kidney fibrosis. J Am Soc Nephrol 21: 212-222.
7. Dai C, Stolz DB, Bastacky SI, St-Arnaud R, Wu C, et al. (2006) Essential role of integrin-linked kinase in podocyte biology: bridging the integrin and slit diaphragm signaling. J Am Soc Nephrol 17: 2164-2175.
8. Morrison AA, Viney RL, Saleem MA, Ladomery MR, (2008) New insights into the function of the Wilms tumor suppressor gene WT1 in podocytes. Am J Physiol Renal Physiol 295: F12-F17.
9. Wagner KD, Wagner N, Schedl A, (2003) The complex life of WT1. J Cell Sci 116: 1653-1658.
10. Palmer RE, Kotsianti A, Cadman B, Boyd T, Gerald W, et al. (2001) WT1 regulates the expression of the major glomerular podocyte membrane protein Podocalyxin. Curr Biol 11: 1805-1809.
11. Wagner N, Wagner KD, Xing Y, Scholz H, Schedl A, (2004) The major podocyte protein nephrin is transcriptionally activated by the Wilms' tumor suppressor WT1. J Am Soc Nephrol 15: 3044-3051.
12. Guo G, Morrison DJ, Licht JD, Quaggin SE, (2004) WT1 activates a glomerular-specific enhancer identified from the human nephrin gene. J Am Soc Nephrol 15: 2851-2856.
13. Guo JK, Menke AL, Gubler MC, Clarke AR, Harrison D, et al. (2002) WT1 is a key regulator of podocyte function: reduced expression levels cause crescentic glomerulonephritis and mesangial sclerosis. Hum Mol Genet 11: 651-659.
14. Lahiri D, Dutton JR, Duarte A, Moorwood K, Graham CF, et al. (2007) Nephropathy and defective spermatogenesis in mice transgenic for a single isoform of the Wilms' tumour suppressor protein, WT1-KTS, together with one disrupted Wt1 allele. Mol Reprod Dev 74: 300-311.
15. Menke AL, IJpenberg A, Fleming S, Ross A, Medine CN, et al. (2003) The wt1-heterozygous mouse; a model to study the development of glomerular sclerosis. J Pathol 200: 667-674.
16. Dai C, Saleem MA, Holzman LB, Mathieson P, Liu Y, (2010) Hepatocyte growth factor signaling ameliorates podocyte injury and proteinuria. Kidney Int 77: 962-973.
17. Macconi D, Bonomelli M, Benigni A, Plati T, Sangalli F, et al. (2006) Pathophysiologic implications of reduced podocyte number in a rat model of progressive glomerular injury. Am J Pathol 168: 42-54.
18. Legate KR, Montanez E, Kudlacek O, Fassler R, (2006) ILK, PINCH and parvin: the tIPP of integrin signalling. Nat Rev Mol Cell Biol 7: 20-31.
19. Wu C, (2005) PINCH, N(i)ck and the ILK: network wiring at cell-matrix adhesions. Trends Cell Biol 15: 460-466.
20. Li Y, Dai C, Wu C, Liu Y, (2007) PINCH-1 promotes tubular epithelial-to-mesenchymal transition by interacting with integrin-linked kinase. J Am Soc Nephrol 18: 2534-2543.
21. Stanchi F, Grashoff C, Nguemeni Yonga CF, Grall D, Fassler R, et al. (2009) Molecular dissection of the ILK-PINCH-parvin triad reveals a fundamental role for the ILK kinase domain in the late stages of focal-adhesion maturation. J Cell Sci 122: 1800-1811.
22. Campana WM, Myers RR, Rearden A, (2003) Identification of PINCH in Schwann cells and DRG neurons: shuttling and signaling after nerve injury. Glia 41: 213-223.
23. Fukuda T, Chen K, Shi X, Wu C, (2003) PINCH-1 is an obligate partner of integrin-linked kinase (ILK) functioning in cell shape modulation, motility, and survival. J Biol Chem 278: 51324-51333.
24. Tu Y, Li F, Goicoechea S, Wu C, (1999) The LIM-only protein PINCH directly interacts with integrin-linked kinase and is recruited to integrin-rich sites in spreading cells. Mol Cell Biol 19: 2425-2434.
25. Yang Y, Wang X, Hawkins CA, Chen K, Vaynberg J, et al. (2009) Structural basis of focal adhesion localization of LIM-only adaptor PINCH by integrin-linked kinase. J Biol Chem 284: 5836-5844.
26. Chiswell BP, Zhang R, Murphy JW, Boggon TJ, Calderwood DA, (2008) The structural basis of integrin-linked kinase-PINCH interactions. Proc Natl Acad Sci U S A 105: 20677-20682.
27. Tu Y, Li F, Wu C, (1998) Nck-2, a novel Src homology2/3-containing adaptor protein that interacts with the LIM-only protein PINCH and components of growth factor receptor kinase-signaling pathways. Mol Biol Cell 9: 3367-3382.
28. Verma R, Kovari I, Soofi A, Nihalani D, Patrie K, et al. (2006) Nephrin ectodomain engagement results in Src kinase activation, nephrin phosphorylation, Nck recruitment, and actin polymerization. J Clin Invest 116: 1346-1359.
29. Jones N, Blasutig IM, Eremina V, Ruston JM, Bladt F, et al. (2006) Nck adaptor proteins link nephrin to the actin cytoskeleton of kidney podocytes. Nature 440: 818-823.
30. Jung KY, Chen K, Kretzler M, Wu C, (2007) TGF-β1 regulates the PINCH-1-integrin-linked kinase-α-parvin complex in glomerular cells. J Am Soc Nephrol 18: 66-73.
31. Bottinger EP, (2007) TGF-β in renal injury and disease. Semin Nephrol 27: 309-320.
32. Nielsen JS, McNagny KM, (2009) The role of podocalyxin in health and disease. J Am Soc Nephrol 20: 1669-1676.
33. Xu Z, Fukuda T, Li Y, Zha X, Qin J, et al. (2005) Molecular dissection of PINCH-1 reveals a mechanism of coupling and uncoupling of cell shape modulation and survival. J Biol Chem 280: 27631-27637.
34. Srichai MB, Konieczkowski M, Padiyar A, Konieczkowski DJ, Mukherjee A, et al. (2004) A WT1 co-regulator controls podocyte phenotype by shuttling between adhesion structures and nucleus. J Biol Chem 279: 14398-14408.
35. Rico M, Mukherjee A, Konieczkowski M, Bruggeman LA, Miller RT, et al. (2005) WT1-interacting protein and ZO-1 translocate into podocyte nuclei after puromycin aminonucleoside treatment. Am J Physiol Renal Physiol 289: F431-F441.
36. Kadrmas JL, Beckerle MC, (2004) The LIM domain: from the cytoskeleton to the nucleus. Nat Rev Mol Cell Biol 5: 920-931.
37. Kim JH, Konieczkowski M, Mukherjee A, Schechtman S, Khan S, et al. (2010) Podocyte injury induces nuclear translocation of WTIP via microtubule-dependent transport. J Biol Chem 285: 9995-10004.
38. Takeda T, McQuistan T, Orlando RA, Farquhar MG, (2001) Loss of glomerular foot processes is associated with uncoupling of podocalyxin from the actin cytoskeleton. J Clin Invest 108: 289-301.
39. Doyonnas R, Kershaw DB, Duhme C, Merkens H, Chelliah S, et al. (2001) Anuria, omphalocele, and perinatal lethality in mice lacking the CD34-related protein podocalyxin. J Exp Med 194: 13-27.
40. Saleem MA, O'Hare MJ, Reiser J, Coward RJ, Inward CD, et al. (2002) A conditionally immortalized human podocyte cell line demonstrating nephrin and podocin expression. J Am Soc Nephrol 13: 630-638.
41. Dai C, Stolz DB, Kiss LP, Monga SP, Holzman LB, et al. (2009) Wnt/β-catenin signaling promotes podocyte dysfunction and albuminuria. J Am Soc Nephrol 20: 1997-2008.
42. Li Y, Tan X, Dai C, Stolz DB, Wang D, et al. (2009) Inhibition of integrin-linked kinase reduces renal interstitial fibrosis. J Am Soc Nephrol 20: 1907-1918.