Histidine Hydrogen-Deuterium Exchange Mass Spectrometry for probing the microenvironment of histidine residues in dihydrofolate reductase

PLoS ONE

Volumn 6, Issue 2, 2011, Pages

  Miyagi, Masaru ^a   Wan, Qun ^a   Ahmad, Md Faiz ^a   Gokulrangan, Giridharan ^a   Tomechko, Sara E ^a   Bennett, Brad ^a,b   Dealwis, Chris ^a  

^a CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF VIRGINIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DIHYDROFOLATE REDUCTASE; HISTIDINE; METHOTREXATE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; DEUTERIUM; HYDROGEN;

ANALYTIC METHOD; ARTICLE; COMPLEX FORMATION; CONFORMATIONAL TRANSITION; CRYSTALLOGRAPHY; DEUTERIUM HYDROGEN EXCHANGE; ENZYME ACTIVE SITE; ESCHERICHIA COLI; LIGAND BINDING; MASS SPECTROMETRY; MICROENVIRONMENT; NEUTRON DIFFRACTION; NONHUMAN; PKA; PROTON NUCLEAR MAGNETIC RESONANCE; PROTON TRANSPORT; STRUCTURE ANALYSIS; BIOLOGICAL MODEL; CHEMICAL STRUCTURE; CHEMISTRY; ENVIRONMENT; ENZYMOLOGY; KINETICS; METABOLISM; METHODOLOGY; NUCLEAR MAGNETIC RESONANCE; PROTEIN BINDING; PROTEIN CONFORMATION;

CATALYTIC DOMAIN; CRYSTALLOGRAPHY; DEUTERIUM; DEUTERIUM EXCHANGE MEASUREMENT; ENVIRONMENT; ESCHERICHIA COLI; HISTIDINE; HYDROGEN; KINETICS; MASS SPECTROMETRY; MODELS, BIOLOGICAL; MODELS, MOLECULAR; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEIN BINDING; PROTEIN CONFORMATION; TETRAHYDROFOLATE DEHYDROGENASE;

EID: 79951890355     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0017055     Document Type: Article

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