An image-based assay for high throughput screening of Giardia lamblia

Journal of Microbiological Methods

Volumn 84, Issue 3, 2011, Pages 398-405

  Gut, Jiri ^a,b   Ang, Kenny K H ^b   Legac, Jennifer ^a,b   Arkin, Michelle R ^a   Rosenthal, Philip J ^a,b   McKerrow, James H ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Giardia lamblia; High content analysis; High throughput screening; In vitro cultivation; Inhibitor

Indexed keywords

ACIVICIN; AKLAVIN; ALBENDAZOLE; ANTIPROTOZOAL AGENT; CYCLOHEXIMIDE; FENBENDAZOLE; FURAZOLIDONE; MEBENDAZOLE; MEPACRINE; METRONIDAZOLE; NITAZOXANIDE; NOCODAZOLE; PSEUDOBAPTIGENIN; RONIDAZOLE; RUTILANTINONE; TINIDAZOLE; UNCLASSIFIED DRUG;

ARTICLE; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG STRUCTURE; GIARDIA LAMBLIA; GROWTH INHIBITION; HIGH THROUGHPUT SCREENING; IC 50; IMAGE ANALYSIS; IN VITRO STUDY; NONHUMAN; PRIORITY JOURNAL; TISSUE CULTURE;

ANIMALS; ANTIPROTOZOAL AGENTS; DRUG EVALUATION, PRECLINICAL; GIARDIA LAMBLIA; HIGH-THROUGHPUT SCREENING ASSAYS; IMAGE PROCESSING, COMPUTER-ASSISTED; PARASITIC SENSITIVITY TESTS;

GIARDIA INTESTINALIS; PROTOZOA;

EID: 79951811068     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2010.12.026     Document Type: Article

References (27)

1. Adagu I.S., Nolder D., Warhurst D.C., Rossignol J. In vitro activity of nitazoxanide and related compounds against isolates of Giardia intestinalis, Entamoeba histolytica and Trichomonas vaginalis. J. Antimicrob. Chemother. 2002, 49:103-111.
2. Argüello-García R., Cruz-Soto M., Romero-Montoya L., Ortega-Pierres G. Variability and variation in drug susceptibility among Giardia duodenalis isolates and clones exposed to 5-nitroimidazoles and benzimidazoles in vitro. J. Antimicrob. Chemother. 2004, 54:711-721.
3. Bénéré E., da Luz R.A., Vermeersch M., Cos P., Maes L. A new quantitative in vitro microculture method for Giardia duodenalis trophozoites. J. Microbiol. Methods 2007, 71:101-106.
4. Bonilla-Santiago R., Wu Z., Zhang L., Widmer G. Identification of growth inhibiting compounds in a Giardia lamblia high-throughput screen. Mol. Biochem. Parasitol. 2008, 162:149-154.
5. Boreham P.F., Phillips R.E., Shepherd R.W. The activity of drugs against Giardia intestinalis in neonatal mice. J. Antimicrob. Chemother. 1986, 18:393-398.
6. Busatti H.G., Gomes M.A. A simple colourimetric method to determine anti-giardial activity of drugs. Parasitol. Res. 2007, 101:819-821.
7. Calzada F., Yépez-Mulia L., Aguilar A. In vitro susceptibility of Entamoeba histolytica and Giardia lamblia to plants used in Mexican traditional medicine for the treatment of gastrointestinal disorders. J. Ethnopharmacol. 2006, 108:367-370.
8. Cruz A., Sousa M.I., Azeredo Z., Leite E., Figueiredo de Sousa J.C., Cabral M. Isolation, excystation and axenization of Giardia lamblia isolates: in vitro susceptibility to metronidazole and albendazole. J. Antimicrob. Chemother. 2003, 51:1017-1020.
9. Diamond L.S., Harlow D.R., Cunnick C.C. A new medium for the axenic cultivation of Entamoeba histolytica and other Entamoeba. Trans. R. Soc. Trop. Med. Hyg. 1978, 72:431-432.
10. Downey A.S., Graczyk T.K., Sullivan D.J. In vitro activity of pyrvinium pamoate against Entamoeba histolytica and Giardia intestinalis using radiolabelled thymidine incorporation and an SYBR Green I-based fluorescence assay. J. Antimicrob. Chemother. 2009, 64:751-754.
11. Engel J.C., Ang K.K.H., Chen S., Arkin M.R., McKerrow J.H., Doyle P.S. Image based high-throughput drug screening targeting the intracellular stage of Trypanosoma cruzi, the agent of Chagas disease. Antimicrob. Agents Chemother. 2010, 54:3326-3334.
12. Faghiri Z., Santiago R.B., Wu Z., Widmer G. High-throughput screening in suboptimal growth conditions identifies agonists of Giardia lamblia proliferation. Parasitology 2010, 1-7. Aug 10. 10.1017/S0031182010001101.
13. Ford B.J. The discovery of Giardia. Microscope 2005, 53:147-153.
14. Irfan I., Sawangjaroen N., Bhat A.R., Azam A. New dioxazole derivatives: Synthesis and effects on the growth of Entamoeba histolytica and Giardia intestinalis. Eur. J. Med. Chem. 2010, 45:1648-1653.
15. Keister D.B. Axenic culture of Giardia lamblia in TYI-S-33 medium supplemented with bile. Trans. R. Soc. Trop. Med. Hyg. 1983, 77:487-488.
16. Khan I.A., Avery M.A., Burandt C.L., Goins D.K., Mikell J.R., Nash T.E., Azadegan A., Walker L.A. Antigiardial activity of isoflavones from Dalbergia frutescens bark. J. Nat. Prod. 2000, 63:1414-1416.
17. Lauwaet T., Andersen Y., Van de Ven L., Eckmann L., Gillin F.D. Rapid detachment of Giardia lamblia trophozoites as a mechanism of antimicrobial action of the isoflavone formononetin. J. Antimicrob. Chemother. 2010, 65:531-534.
18. Mackey Z.B., Baca A.M., Mallari J.P., Apsel B., Shelat A., Hansell E.J., Chiang P.K., Wolff B., Guy K.R., Williams J., McKerrow J.H. Discovery of trypanocidal compounds by whole cell HTS of Trypanosoma brucei. Chem. Biol. Drug Des. 2006, 67:355-363.
19. Manna D., Dutta P.K., Achari B., Lohia A. A novel galacto-glycerolipid from Oxalis corniculata kills Entamoeba histolytica and Giardia lamblia. Antimicrob. Agents Chemother. 2010, Aug 16. 10.1128/AAC.00546-10.
20. Mariante R.M., Vancini R.G., Melo A.L., Benchimol M. Giardia lamblia: evaluation of the in vitro effects of nocodazole and colchicine on trophozoites. Exp. Parasitol. 2005, 110:62-72.
21. Morgan U.M., Reynoldson J.A., Thompson R.C. Activities of several benzimidazoles and tubulin inhibitors against Giardia spp. in vitro. Antimicrob. Agents Chemother. 1993, 37:328-331.
22. Robertson L.J., Hanevik K., Escobedo A.A., Mørch K., Langeland N. Giardiasis: why do the symptoms sometimes never stop?. Trends Parasitol. 2010, 26:75-82.
23. Sawangjaroen N., Subhadhirasakul S., Phongpaichit S., Siripanth C., Jamjaroen K., Sawangjaroen K. The in vitro anti-giardial activity of extracts from plants that are used for self-medication by AIDS patients in southern Thailand. Parasitol. Res. 2005, 95:17-21.
24. Sharlow E.R., Close D., Shun T., Leimgruber S., Reed R., Mustata G., Wipf P., Johnson J., O'Neil M., Grögl M., Magill A.J., Lazo J.S. Identification of potent chemotypes targeting Leishmania major using a high-throughput, low-stringency, computationally enhanced, small molecule screen. PLoS Negl.Trop. Dis. 2009, 3:e540.
25. Upcroft J.A., Upcroft P. Drug Susceptibility Testing of Anaerobic Protozoa. Antimicrob. Agents Chemother. 2001, 45:1810-1814.
26. Wishart D.S., Knox C., Guo A.C., Cheng D., Shrivastava S., Tzur D., Gautam B., Hassanali M. DrugBank: a knowledgebase for drugs, drug actions and drug targets. Nucleic Acids Res. 2008, 36:D901-D906.
27. Zhang J.H., Chung T.D., Oldenburg K.R. A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J. Biol. Screen. 1999, 4:67-73.