Reduced glutathione content in human sperm is decreased after cryopreservation: Effect of the addition of reduced glutathione to the freezing and thawing extenders

Cryobiology

Volumn 62, Issue 1, 2011, Pages 40-46

  Gadea, J ^a   Molla, M ^a,b   Selles, E ^a,b   Marco, M A ^a   Garcia Vazquez, F A ^a   Gardon, J C ^a  

^a UNIVERSITY OF MURCIA   (Spain)

^b IVI Murcia   (Spain)

Author keywords

Antioxidant; Cryopreservation; Glutathione; Oxidative stress; Spermatozoa

Indexed keywords

CRYOPROTECTIVE AGENT; GLUTATHIONE; MEMBRANE PROTEIN; REACTIVE OXYGEN METABOLITE; THIOL GROUP;

ARTICLE; CELL LEVEL; CELL VIABILITY; CHROMATIN CONDENSATION; CONTROLLED STUDY; CRYOPRESERVATION; FREEZE THAWING; HUMAN; HUMAN CELL; LIPID MEMBRANE; MALE; PRIORITY JOURNAL; SPERM; SPERMATOZOON MOTILITY;

ANTIOXIDANTS; CRYOPRESERVATION; CRYOPROTECTIVE AGENTS; FREEZING; GLUTATHIONE; HUMANS; MALE; REACTIVE OXYGEN SPECIES; SEMEN ANALYSIS; SEMEN PRESERVATION; SPERM MOTILITY; SPERMATOZOA; SULFHYDRYL COMPOUNDS;

EID: 79951516239     PISSN: 00112240     EISSN: 10902392     Source Type: Journal    
DOI: 10.1016/j.cryobiol.2010.12.001     Document Type: Article

References (57)

1. Agarwal A., Gupta S., Sikka S. The role of free radicals and antioxidants in reproduction. Current Opinion in Obstetrics & Gynecology 2006, 18:325.
2. Agarwal A., Sharma R.K., Nallella K.P., Thomas A.J., Alvarez J.G., Sikka S.C. Reactive oxygen species as an independent marker of male factor infertility. Fertility and Sterility 2006, 86:878.
3. Aitken R.J. The role of free oxygen radicals and sperm function. International Journal of Andrology 1989, 12:95.
4. Aitken R.J. Free radicals, lipid peroxidation and sperm function. Reproduction, Fertility, and Development 1995, 7:659.
5. Alvarez J.G., Storey B.T. Role of glutathione peroxidase in protecting mammalian spermatozoa from loss of motility caused by spontaneous lipid peroxidation. Gamete Research 1989, 23:77.
6. Alvarez J.G., Storey B.T. Evidence for increased lipid peroxidative damage and loss of superoxide dismutase activity as a mode of sublethal cryodamage to human sperm during cryopreservation. Journal of Andrology 1992, 13:232.
7. Alvarez J.G., Storey B.T. Evidence that membrane stress contributes more than lipid peroxidation to sublethal cryodamage in cryopreserved human sperm: glycerol and other polyols as sole cryoprotectant. Journal of Andrology 1993, 14:199.
8. Alvarez J.G., Touchstone J.C., Blasco L., Storey B.T. Spontaneous lipid peroxidation and production of hydrogen peroxide and superoxide in human spermatozoa, superoxide dismutase as major enzyme protectant against oxygen toxicity. Journal of andrology 1987, 8:338.
9. Anger J.T., Gilbert B.R., Goldstein M. Cryopreservation of sperm: indications, methods and results. Journal of Urology 2003, 170:1079-1084.
10. Bhardwaj A., Verma A., Majumdar S., Khanduja K.L. Status of vitamin E and reduced glutathione in semen of oligozoospermic and azoospermic patients. Asian Journal of Andrology 2000, 2:225.
11. Bilodeau J.F., Chatterjee S., Sirard M.A., Gagnon C. Levels of antioxidant defenses are decreased in bovine spermatozoa after a cycle of freezing and thawing. Molecular Reproduction and Development 2000, 55:282.
12. 2-mediated loss of sperm motility in cryopreserved bull semen. Theriogenology 2001, 56:275.
13. Brotherton J. Cryopreservation of human semen. Archives of Andrology 1990, 25:181.
14. Chatterjee S., Gagnon C. Production of reactive oxygen species by spermatozoa undergoing cooling, freezing, and thawing. Molecular Reproduction and Development 2001, 59:451.
15. Chatterjee S., de Lamirande E., Gagnon C. Cryopreservation alters membrane sulfhydryl status of bull spermatozoa: protection by oxidized glutathione. Molecular Reproduction and Development 2001, 60:498.
16. Donnelly E.T., McClure N., Lewis S.E. Glutathione and hypotaurine in vitro: effects on human sperm motility, DNA integrity and production of reactive oxygen species. Mutagenesis 2000, 15:61.
17. Floreani M., Petrone M., Debetto P., Palatini P. A comparison between different methods for the determination of reduced and oxidized glutathione in mammalian tissues. Free Radical Research 1997, 26:449.
18. Foote R.H., Brockett C.C., Kaproth M.T. Motility and fertility of bull sperm in whole milk extender containing antioxidants. Animal Reproduction Science 2002, 71:13.
19. Funahashi H., Sano T. Select antioxidants improve the function of extended boar semen stored at 10°C. Theriogenology 2005, 63:1605.
20. Gadea J., Selles E., Marco M.A., Coy P., Matas C., Romar R., Ruiz S. Decrease in glutathione content in boar sperm after cryopreservation. Effect of the addition of reduced glutathione to the freezing and thawing extenders. Theriogenology 2004, 62:690.
21. Gadea J., Selles E., Marco M.A. The predictive value of porcine seminal parameters on fertility outcome under commercial conditions. Reproduction in domestic animals. Zuchthygiene 2004, 39:303.
22. Gadea J., Garcia-Vazquez F., Matas C., Gardon J.C., Canovas S., Gumbao D. Cooling and freezing of boar spermatozoa: supplementation of the freezing media with reduced glutathione preserves sperm function. Journal of Andrology 2005, 26:396.
23. Gadea J., Gumbao D., Matas C., Romar R. Supplementation of the thawing media with reduced glutathione improves function and the in vitro fertilizing ability of boar spermatozoa after cryopreservation. Journal of Andrology 2005, 26:749.
24. Gadea J., Gumbao D., Canovas S., Garcia-Vazquez F.A., Grullon L.A., Gardon J.C. Supplementation of the dilution medium after thawing with reduced glutathione improves function and the in vitro fertilizing ability of frozen-thawed bull spermatozoa. International Journal of Andrology 2008, 31:40.
25. Garrido N., Meseguer M., Alvarez J., Simon C., Pellicer A., Remohi J. Relationship among standard semen parameters, glutathione peroxidase/glutathione reductase activity, and mRNA expression and reduced glutathione content in ejaculated spermatozoa from fertile and infertile men. Fertility and Sterility 2004, 82(Suppl. 3):1059.
26. Gil-Salom M., Romero J., Minguez Y., Rubio C., De los Santos M.J., Remohi J., Pellicer A. Pregnancies after intracytoplasmic sperm injection with cryopreserved testicular spermatozoa. Human reproduction (Oxford, England) 1996, 11:1309.
27. Griveau J.F., Lelannou D. Effects of antioxidants on human sperm preparation techniques. International Journal of Andrology 1994, 17:225-231.
28. Hamamah S., Royere D., Nicolle J.C., Paquignon M., Lansac J. Effects of freezing-thawing on the spermatozoon nucleus: a comparative chromatin cytophotometric study in the porcine and human species. Reproduction, Nutrition, Development 1990, 30:59.
29. Hammadeh M.E., Askari A.S., Georg T., Rosenbaum P., Schmidt W. Effect of freeze-thawing procedure on chromatin stability, morphological alteration and membrane integrity of human spermatozoa in fertile and subfertile men. International Journal of Andrology 1999, 22:155.
30. 2, an effector of capacitation, induces a rapid and reversible change in the lipid architecture of boar sperm plasma membranes. Molecular Reproduction and Development 1996, 45:378.
31. Hemachand T., Shaha C. Functional role of sperm surface glutathione S-transferases and extracellular glutathione in the haploid spermatozoa under oxidative stress. FEBS Letters 2003, 538:14-18.
32. Jeulin C., Soufir J.C., Weber P., Laval-Martin D., Calvayrac R. Catalase activity in human spermatozoa and seminal plasma. Gamete Research 1989, 24:185.
33. Kumar G.P., Laloraya M., Laloraya M.M. Powerful anti-sperm motility action of cobaltous ion and its recovery by a sulfhydryl compound. Contraception 1990, 41:633-639.
34. Lasso J.L., Noiles E.E., Alvarez J.G., Storey B.T. Mechanism of superoxide dismutase loss from human sperm cells during cryopreservation. Journal of Andrology 1994, 15:255.
35. Li T.K. The glutathione and thiol content of mammalian spermatozoa and seminal plasma. Biology of Reproduction 1975, 12:641.
36. Z. Li, Q. Lin, R. Liu, W. Xiao, W. Liu, Protective effects of ascorbate and catalase on human spermatozoa during cryopreservation. Journal of Andrology (2009), jandrol.109.007849.
37. Martinez-Soto J.C., de DiosHourcade J., Gutierrez-Adan A., Landeras J.L., Gadea J. Effect of genistein supplementation of thawing medium on characteristics of frozen human spermatozoa. Asian Journal of Andrology 2010, 12:431-441.
38. Maxwell W.M., Landers A.J., Evans G. Survival and fertility of ram spermatozoa frozen in pellets, straws and minitubes. Theriogenology 1995, 43:1201.
39. Mazur P. Freezing of living cells: mechanisms and implications. Am J Physiol Cell Physiol 1984, 247:C125-142.
40. Meseguer M., Garrido N., Martinez-Conejero J.A., Simon C., Pellicer A., Remohi J. Role of cholesterol, calcium, and mitochondrial activity in the susceptibility for cryodamage after a cycle of freezing and thawing. Fertility and Sterility 2004, 81:588.
41. Meseguer M., Garrido N., Simon C., Pellicer A., Remohi J. Concentration of glutathione and expression of glutathione peroxidases 1 and 4 in fresh sperm provide a forecast of the outcome of cryopreservation of human spermatozoa. Journal of Andrology 2004, 25:773.
42. Ochsendorf F.R., Buhl R., Bastlein A., Beschmann H. Glutathione in Spermatozoa and seminal plasma of infertile men. Human Reproduction (Oxford, England) 1998, 13:353.
43. O'Connell M., McClure N., Lewis S. The effects of cryopreservation on sperm morphology, motility and mitochondrial function. Human Reproduction 2002, 17:704.
44. Oehninger S., Duru N.K., Srisombut C., Morshedi M. Assessment of sperm cryodamage and strategies to improve outcome. Molecular and Cellular Endocrinology 2000, 169:3.
45. Parinaud J., Le Lannou D., Vieitez G., Griveau J.F., Milhet P., Richoilley G. Enhancement of motility by treating spermatozoa with an antioxidant solution (Sperm-Fit) following ejaculation. Human Reproduction (Oxford, England) 1997, 12:2434.
46. Raijmakers M.T., Roelofs H.M., Steegers E.A., Steegers-Theunissen R.R.P., Mulder T.P., Knapen M.F., Wong W.Y., Peters W.H. Glutathione and glutathione S-transferases A1-1 and P1-1 in seminal plasma may play a role in protecting against oxidative damage to spermatozoa. Fertility and Sterility 2003, 79:169.
47. Rossi T., Mazzilli F., Delfino M., Dondero F. Improved human sperm recovery using superoxide dismutase and catalase supplementation in semen cryopreservation procedure. Cell and Tissue Banking 2001, 2:9-13.
48. Schiller J., Arnhold J., Glander H.J., Arnold K. Lipid analysis of human spermatozoa and seminal plasma by MALDI-TOF mass spectrometry and NMR spectroscopy - effects of freezing and thawing. Chemistry and Physics of Lipids 2000, 106:145.
49. Sinha S., Pradeep Kumar G., Laloraya M., Warikoo D. Over-expression of superoxide dismutase and lack of surface-thiols in spermatozoa: inherent defects in oligospermia. Biochemical and Biophysical Research Communications 1991, 174:510-517.
50. Stradaioli G., Noro T., Sylla L., Monaci M. Decrease in glutathione (GSH) content in bovine sperm after cryopreservation: comparison between two extenders. Theriogenology 2007, 67:1249.
51. Taylor K., Roberts P., Sanders K., Burton P. Effect of antioxidant supplementation of cryopreservation medium on post-thaw integrity of human spermatozoa. Reproductive Biomedicine Online 2009, 18:184-189.
52. Therond P., Auger J., Legrand A., Jouannet P. Alpha-Tocopherol in human spermatozoa and seminal plasma: relationships with motility, antioxidant enzymes and leukocytes. Molecular Human Reproduction 1996, 2:739-744.
53. Thomson L.K., Fleming S.D., Aitken R.J., De Iuliis G.N., Zieschang J.-A., Clark A.M. Cryopreservation-induced human sperm DNA damage is predominantly mediated by oxidative stress rather than apoptosis. Human Reproduction 2009, 24:2061-2070.
54. Varghese A.C., Das S., Bhattacharya A.K., Bhattacharya S.M., Mandal M., Agarwal A. Effect of cryoprotective additives-reduced glutathione, acetyl-l-carnitine on sperm membrane lipid peroxidation, DNA integrity and recovery of motile human sperm. Fertility and Sterility 2005, 84:S410-S411.
55. Wang A.W., Zhang H., Ikemoto I., Anderson D.J., Loughlin K.R. Reactive oxygen species generation by seminal cells during cryopreservation. Urology 1997, 49:921-925.
56. White I.G. Lipids and calcium uptake of sperm in relation to cold shock and preservation: a review. Reproduction, Fertility, and Development 1993, 5:639.
57. WHO, WHO Laboratory Manual for the Examination of Human Semen and Sperm-Cervical Mucus Interaction, fourth ed., Cambridge University Press, Cambridge, UK, 1999.