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Volumn 12, Issue 3, 2010, Pages 431-441

Effect of genistein supplementation of thawing medium on characteristics of frozen human spermatozoa

Author keywords

Antioxidants; Cryopreservation; DNA damage; Genistein; Isoflavones; Sperm motility

Indexed keywords

G 6649; GENISTEIN; REACTIVE OXYGEN METABOLITE; UNCLASSIFIED DRUG;

EID: 77952048174     PISSN: 1008682X     EISSN: 17457262     Source Type: Journal    
DOI: 10.1038/aja.2009.92     Document Type: Article
Times cited : (55)

References (56)
  • 2
    • 0036123156 scopus 로고    scopus 로고
    • The effects of cryopreservation on sperm morphology, motility and mitochondrial function
    • O'Connell M, McClure N, Lewis SE. The effects of cryopreservation on sperm morphology, motility and mitochondrial function. Hum Reprod 2002;17:704-9.
    • (2002) Hum. Reprod. , vol.17 , pp. 704-709
    • O'Connell, M.1    McClure, N.2    Lewis, S.E.3
  • 3
    • 0033868942 scopus 로고    scopus 로고
    • Lipid analysis of human spermatozoa and seminal plasma by MALDI-TOF mass spectrometry and NMR spectroscopy-effects of freezing and thawing
    • Schiller J, Arnhold J, Glander HJ, Arnold K. Lipid analysis of human spermatozoa and seminal plasma by MALDI-TOF mass spectrometry and NMR spectroscopy-effects of freezing and thawing. Chem Phys Lipids 2000;106:145-56.
    • (2000) Chem. Phys. Lipids , vol.106 , pp. 145-156
    • Schiller, J.1    Arnhold, J.2    Glander, H.J.3    Arnold, K.4
  • 4
    • 0023241776 scopus 로고
    • Cryopreservation of human spermatozoa. II. Postthaw chronology of motility and of zona-free hamster ova penetration
    • Critser JK, Arneson BW, Aaker DV, Huse-Benda AR, Ball GD. Cryopreservation of human spermatozoa. II. postthaw chronology of motility and of zona-free hamster ova penetration. Fertil Steril 1987;47:980-4.
    • (1987) Fertil. Steril. , vol.47 , pp. 980-984
    • Critser, J.K.1    Arneson, B.W.2    Aaker, D.V.3    Huse-Benda, A.R.4    Ball, G.D.5
  • 5
    • 0027237261 scopus 로고
    • Evidence that membrane stress contributes more than lipid peroxidation to sublethal cryodamage in cryopreserved human sperm: Glycerol and other polyols as sole cryoprotectant
    • Alvarez JG, Storey BT. Evidence that membrane stress contributes more than lipid peroxidation to sublethal cryodamage in cryopreserved human sperm: glycerol and other polyols as sole cryoprotectant. J Androl 1993;14:199-209.
    • (1993) J. Androl. , vol.14 , pp. 199-209
    • Alvarez, J.G.1    Storey, B.T.2
  • 6
    • 0026672788 scopus 로고
    • The contribution of the toxicity of a glycerol-egg yolk-citrate cryopreservative to the decline in human sperm motility during cryopreservation
    • McLaughlin EA, Ford WC, Hull MG. The contribution of the toxicity of a glycerol-egg yolk-citrate cryopreservative to the decline in human sperm motility during cryopreservation. J Reprod Fertil 1992;95:749-54.
    • (1992) J. Reprod. Fertil. , vol.95 , pp. 749-754
    • McLaughlin, E.A.1    Ford, W.C.2    Hull, M.G.3
  • 7
    • 0023512988 scopus 로고
    • Acrosomal enzymes and ultrastructure of unfrozen and cryotreated human spermatozoa
    • Mack SR, Zaneveld LJ. Acrosomal enzymes and ultrastructure of unfrozen and cryotreated human spermatozoa. Gamete Res 1987;18:375-83.
    • (1987) Gamete Res. , vol.18 , pp. 375-383
    • Mack, S.R.1    Zaneveld, L.J.2
  • 8
    • 0034963068 scopus 로고    scopus 로고
    • Production of reactive oxygen species by spermatozoa undergoing cooling, freezing, and thawing
    • Chatterjee S, Gagnon C. Production of reactive oxygen species by spermatozoa undergoing cooling, freezing, and thawing. Mol Reprod Dev 2001;59:451-8.
    • (2001) Mol. Reprod. Dev. , vol.59 , pp. 451-458
    • Chatterjee, S.1    Gagnon, C.2
  • 9
    • 0024477563 scopus 로고
    • The role of free oxygen radicals and sperm function
    • Aitken RJ. The role of free oxygen radicals and sperm function. Int J Androl 1989;12:95-7.
    • (1989) Int. J. Androl. , vol.12 , pp. 95-97
    • Aitken, R.J.1
  • 10
    • 0028891932 scopus 로고
    • Differential incorporation of fatty acids into and peroxidative loss of fatty acids from phospholipids of human spermatozoa
    • Alvarez JG, Storey BT. Differential incorporation of fatty acids into and peroxidative loss of fatty acids from phospholipids of human spermatozoa. Mol Reprod Dev 1995;42:334-46.
    • (1995) Mol. Reprod. Dev. , vol.42 , pp. 334-346
    • Alvarez, J.G.1    Storey, B.T.2
  • 11
    • 0027898335 scopus 로고
    • Lipids and calcium uptake of sperm in relation to cold shock and preservation: A review
    • White IG. Lipids and calcium uptake of sperm in relation to cold shock and preservation: a review. Reprod Fertil Dev 1993;5:639-58.
    • (1993) Reprod. Fertil. Dev. , vol.5 , pp. 639-658
    • White, I.G.1
  • 12
    • 0029451739 scopus 로고
    • Free radicals, lipid peroxidation and sperm function
    • Aitken RJ. Free radicals, lipid peroxidation and sperm function. Reprod Fertil Dev 1995;7:659-68.
    • (1995) Reprod. Fertil. Dev. , vol.7 , pp. 659-668
    • Aitken, R.J.1
  • 13
  • 14
    • 0031778295 scopus 로고    scopus 로고
    • Regulation of protein phosphorylation during sperm capacitation
    • Visconti PE, Kopf GS. Regulation of protein phosphorylation during sperm capacitation. Biol Reprod 1998;59:1-6.
    • (1998) Biol. Reprod. , vol.59 , pp. 1-6
    • Visconti, P.E.1    Kopf, G.S.2
  • 15
    • 0035820073 scopus 로고    scopus 로고
    • Effect of phytoestrogen and antioxidant supplementation on oxidative DNA damage assessed using the comet assay
    • Sierens J, Hartley JA, Campbell MJ, Leathem AJ, Woodside JV. Effect of phytoestrogen and antioxidant supplementation on oxidative DNA damage assessed using the comet assay. Mutat Res 2001;485:169-76.
    • (2001) Mutat. Res. , vol.485 , pp. 169-176
    • Sierens, J.1    Hartley, J.A.2    Campbell, M.J.3    Leathem, A.J.4    Woodside, J.V.5
  • 18
    • 0029461068 scopus 로고
    • Recent developments and concepts in the cryopreservation of spermatozoa and the assessment of their post-thawing function
    • Watson PF. Recent developments and concepts in the cryopreservation of spermatozoa and the assessment of their post-thawing function. Reprod Fertil Dev 1995;7:871-91.
    • (1995) Reprod. Fertil. Dev. , vol.7 , pp. 871-891
    • Watson, P.F.1
  • 19
    • 79961199198 scopus 로고    scopus 로고
    • Effect of tyrosine kinase inhibitors on tyrosine phosphorylation and motility parameters in human sperm
    • Bajpai M, Asin S, Doncel GF. Effect of tyrosine kinase inhibitors on tyrosine phosphorylation and motility parameters in human sperm. Arch Androl 2003;49:229-46. (Pubitemid 38223322)
    • (2003) Archives of Andrology , vol.49 , Issue.3 , pp. 229-246
    • Bajpai, M.1    Asin, S.2    Doncel, G.F.3
  • 20
    • 0042025019 scopus 로고    scopus 로고
    • Involvement of tyrosine kinase and cAMP-dependent kinase cross-talk in the regulation of human sperm motility
    • Bajpai M, Doncel GF. Involvement of tyrosine kinase and cAMP-dependent kinase cross-talk in the regulation of human sperm motility. Reproduction 2003;126:183-95. (Pubitemid 36987164)
    • (2003) Reproduction , vol.126 , Issue.2 , pp. 183-195
    • Bajpai, M.1    Doncel, G.F.2
  • 21
    • 77952059407 scopus 로고    scopus 로고
    • World Health Organization. WHO Laboratory Manual for the Examination of Human Semen and Sperm-Cervical Mucus interaction, 4th ed. Cambridge: Cambridge University Press
    • World Health Organization. WHO Laboratory Manual for the Examination of Human Semen and Sperm-Cervical Mucus interaction, 4th ed. Cambridge: Cambridge University Press; 1999. p128.
    • (1999) , pp. 128
  • 22
    • 1642277862 scopus 로고    scopus 로고
    • Role of cholesterol, calcium, and mitochondrial activity in the susceptibility for cryodamage after a cycle of freezing and thawing
    • Meseguer M, Garrido N, Martinez-Conejero JA, Simon C, Pellicer A, et al. Role of cholesterol, calcium, and mitochondrial activity in the susceptibility for cryodamage after a cycle of freezing and thawing. Fertil Steril 2004;81:588-94.
    • (2004) Fertil. Steril. , vol.81 , pp. 588-594
    • Meseguer, M.1    Garrido, N.2    Martinez-Conejero, J.A.3    Simon, C.4    Pellicer, A.5
  • 23
    • 4143154043 scopus 로고    scopus 로고
    • Concentration of glutathione and expression of glutathione peroxidases 1 and 4 in fresh sperm provide a forecast of the outcome of cryopreservation of human spermatozoa
    • Meseguer M, Garrido N, Simon C, Pellicer A, Remohi J. Concentration of glutathione and expression of glutathione peroxidases 1 and 4 in fresh sperm provide a forecast of the outcome of cryopreservation of human spermatozoa. J Androl 2004;25:773-80.
    • (2004) J. Androl. , vol.25 , pp. 773-780
    • Meseguer, M.1    Garrido, N.2    Simon, C.3    Pellicer, A.4    Remohi, J.5
  • 25
    • 84907132684 scopus 로고
    • Cryopreservation of human semen
    • Brotherton J. Cryopreservation of human semen. Arch Androl 1990;25:181-95.
    • (1990) Arch. Androl. , vol.25 , pp. 181-195
    • Brotherton, J.1
  • 26
    • 0034683170 scopus 로고    scopus 로고
    • Basic aspects of frozen storage of semen
    • Holt WV. Basic aspects of frozen storage of semen. Anim Reprod Sci 2000;62:3-22.
    • (2000) Anim. Reprod. Sci. , vol.62 , pp. 3-22
    • Holt, W.V.1
  • 27
    • 0001554959 scopus 로고
    • Survival and fertility of ram spermatozoa frozen in pellets, straws and minitubes
    • Maxwell WM, Landers AJ, Evans G. Survival and fertility of ram spermatozoa frozen in pellets, straws and minitubes. Theriogenology 1995;43:1201-10.
    • (1995) Theriogenology , vol.43 , pp. 1201-1210
    • Maxwell, W.M.1    Landers, A.J.2    Evans, G.3
  • 28
    • 0029657896 scopus 로고    scopus 로고
    • 2, an effector of capacitation, induces a rapid and reversible change in the lipid architecture of boar sperm plasma membranes
    • 2, an effector of capacitation, induces a rapid and reversible change in the lipid architecture of boar sperm plasma membranes. Mol Reprod Dev 1996;45:378-91.
    • (1996) Mol. Reprod. Dev. , vol.45 , pp. 378-391
    • Harrison, R.A.1    Ashworth, P.J.2    Miller, N.G.3
  • 29
    • 18244363853 scopus 로고    scopus 로고
    • Cooling and freezing of boar spermatozoa: Supplementation of the freezing media with reduced glutathione preserves sperm function
    • DOI 10.2164/jandrol.04155
    • Gadea J, Garcia-Vazquez F, Matas C, Gardon JC, Canovas S, et al. Cooling and freezing of boar spermatozoa: supplementation of the freezing media with reduced glutathione preserves sperm function. J Androl 2005;26:396-404. (Pubitemid 40628977)
    • (2005) Journal of Andrology , vol.26 , Issue.3 , pp. 396-404
    • Gadea, J.1    Garcia-Vazquez, F.2    Matas, C.3    Gardon, J.C.4    Canovas, S.5    Gumbao, D.6
  • 30
    • 26944483996 scopus 로고    scopus 로고
    • Supplementation of the thawing media with reduced glutathione improves function and the in vitro fertilizing ability of boar spermatozoa after cryopreservation
    • Gadea J, Gumbao D, Matas C, Romar R. Supplementation of the thawing media with reduced glutathione improves function and the in vitro fertilizing ability of boar spermatozoa after cryopreservation. J Androl 2005;26:749-56.
    • (2005) J. Androl. , vol.26 , pp. 749-756
    • Gadea, J.1    Gumbao, D.2    Matas, C.3    Romar, R.4
  • 31
    • 38049074486 scopus 로고    scopus 로고
    • Supplementation of the dilution medium after thawing with reduced glutathione improves function and the in vitro fertilizing ability of frozen-thawed bull spermatozoa
    • Gadea J, Gumbao D, Canovas S, Garcia-Vazquez FA, Grullon LA, et al. Supplementation of the dilution medium after thawing with reduced glutathione improves function and the in vitro fertilizing ability of frozen-thawed bull spermatozoa. Int J Androl 2008;31:40-9.
    • (2008) Int. J. Androl. , vol.31 , pp. 40-49
    • Gadea, J.1    Gumbao, D.2    Canovas, S.3    Garcia-Vazquez, F.A.4    Grullon, L.A.5
  • 32
    • 41549124198 scopus 로고    scopus 로고
    • Long-term effects of mouse intracytoplasmic sperm injection with DNA-fragmented sperm on health and behavior of adult offspring
    • Fernandez-Gonzalez R, Moreira PN, Perez-Crespo M, Sanchez-Martin M, Ramirez MA, et al. Long-term effects of mouse intracytoplasmic sperm injection with DNA-fragmented sperm on health and behavior of adult offspring. Biol Reprod 2008;78:761-72.
    • (2008) Biol. Reprod. , vol.78 , pp. 761-772
    • Fernandez-Gonzalez, R.1    Moreira, P.N.2    Perez-Crespo, M.3    Sanchez-Martin, M.4    Ramirez, M.A.5
  • 33
    • 0026703651 scopus 로고
    • Evidence for increased lipid peroxidative damage and loss of superoxide dismutase activity as a mode of sublethal cryodamage to human sperm during cryopreservation
    • Alvarez JG, Storey BT. Evidence for increased lipid peroxidative damage and loss of superoxide dismutase activity as a mode of sublethal cryodamage to human sperm during cryopreservation. J Androl 1992;13:232-41.
    • (1992) J. Androl. , vol.13 , pp. 232-241
    • Alvarez, J.G.1    Storey, B.T.2
  • 34
    • 0029896377 scopus 로고    scopus 로고
    • Development of an image analysis system to monitor the retention of residual cytoplasm by human spermatozoa: Correlation with biochemical markers of the cytoplasmic space, oxidative stress, and sperm function
    • Gomez E, Buckingham DW, Brindle J, Lanzafame F, Irvine DS, et al. Development of an image analysis system to monitor the retention of residual cytoplasm by human spermatozoa: correlation with biochemical markers of the cytoplasmic space, oxidative stress, and sperm function. J Androl 1996;17:276-87.
    • (1996) J. Androl. , vol.17 , pp. 276-287
    • Gomez, E.1    Buckingham, D.W.2    Brindle, J.3    Lanzafame, F.4    Irvine, D.S.5
  • 35
    • 0030820190 scopus 로고    scopus 로고
    • Reactive oxygen species and human spermatozoa: Physiology and pathology
    • Griveau JF, Le Lannou D. Reactive oxygen species and human spermatozoa: physiology and pathology. Int J Androl 1997;20:61-9.
    • (1997) Int. J. Androl. , vol.20 , pp. 61-69
    • Griveau, J.F.1    Le Lannou, D.2
  • 36
    • 0030863554 scopus 로고    scopus 로고
    • Effect of various genotoxins and reproductive toxins in human lymphocytes and sperm in the comet assay
    • Anderson D, Dobrzynska MM, Basaran N. Effect of various genotoxins and reproductive toxins in human lymphocytes and sperm in the comet assay. Teratog Carcinog Mutagen 1997;17:29-43.
    • (1997) Teratog Carcinog Mutagen , vol.17 , pp. 29-43
    • Anderson, D.1    Dobrzynska, M.M.2    Basaran, N.3
  • 37
  • 38
    • 12344309281 scopus 로고    scopus 로고
    • Modulation by flavonoids of DNA damage induced by estrogen-like compounds
    • Cemeli E, Schmid TE, Anderson D. Modulation by flavonoids of DNA damage induced by estrogen-like compounds. Environ Mol Mutagen 2004;44:420-6.
    • (2004) Environ. Mol. Mutagen , vol.44 , pp. 420-426
    • Cemeli, E.1    Schmid, T.E.2    Anderson, D.3
  • 39
    • 42649139906 scopus 로고    scopus 로고
    • Impact of estrogenic compounds on DNA integrity in human spermatozoa: Evidence for cross-linking and redox cycling activities
    • Bennetts LE, De Iuliis GN, Nixon B, Kime M, Zelski K, et al. Impact of estrogenic compounds on DNA integrity in human spermatozoa: evidence for cross-linking and redox cycling activities. Mutat Res 2008;641:1-11.
    • (2008) Mutat. Res. , vol.641 , pp. 1-11
    • Bennetts, L.E.1    De Iuliis, G.N.2    Nixon, B.3    Kime, M.4    Zelski, K.5
  • 40
    • 0042009753 scopus 로고    scopus 로고
    • Quantitative analysis of gene-specific DNA damage in human spermatozoa
    • Sawyer DE, Mercer BG, Wiklendt AM, Aitken RJ. Quantitative analysis of gene-specific DNA damage in human spermatozoa. Mutat Res 2003;529:21-34.
    • (2003) Mutat. Res. , vol.529 , pp. 21-34
    • Sawyer, D.E.1    Mercer, B.G.2    Wiklendt, A.M.3    Aitken, R.J.4
  • 41
    • 68949206432 scopus 로고    scopus 로고
    • Cryopreservation-induced human sperm DNA damage is predominantly mediated by oxidative stress rather than apoptosis
    • Thomson LK, Fleming SD, Aitken RJ, De Iuliis GN, Zieschang JA, et al. Cryopreservation-induced human sperm DNA damage is predominantly mediated by oxidative stress rather than apoptosis. Hum Reprod 2009;24:2061-70.
    • (2009) Hum. Reprod. , vol.24 , pp. 2061-2070
    • Thomson, L.K.1    Fleming, S.D.2    Aitken, R.J.3    De Iuliis, G.N.4    Zieschang, J.A.5
  • 42
    • 0025216941 scopus 로고
    • Effects of freezing-thawing on the spermatozoon nucleus: A comparative chromatin cytophotometric study in the porcine and human species
    • Hamamah S, Royere D, Nicolle JC, Paquignon M, Lansac J. Effects of freezing-thawing on the spermatozoon nucleus: a comparative chromatin cytophotometric study in the porcine and human species. Reprod Nutr Dev 1990;30:59-64.
    • (1990) Reprod. Nutr. Dev. , vol.30 , pp. 59-64
    • Hamamah, S.1    Royere, D.2    Nicolle, J.C.3    Paquignon, M.4    Lansac, J.5
  • 43
    • 0035983564 scopus 로고    scopus 로고
    • In vitro fertilizing capacity and chromatin condensation of deep frozen boar semen packaged in 0.5 and 5 mL straws
    • Cordova A, Perez-Gutierrez JF, Lleo B, Garcia-Artiga C, Alvarez A, et al. In vitro fertilizing capacity and chromatin condensation of deep frozen boar semen packaged in 0.5 and 5 mL straws. Theriogenology 2002;57:2119-28.
    • (2002) Theriogenology , vol.57 , pp. 2119-2128
    • Cordova, A.1    Perez-Gutierrez, J.F.2    Lleo, B.3    Garcia-Artiga, C.4    Alvarez, A.5
  • 45
    • 0009750527 scopus 로고
    • The oxidation of sulphydryl compounds by hydrogen peroxide: Catalysis of oxidation of cysteine and glutathione by iron and copper
    • Pirie NW. The oxidation of sulphydryl compounds by hydrogen peroxide: catalysis of oxidation of cysteine and glutathione by iron and copper. Biochem J 1931;25:1565-79.
    • (1931) Biochem. J. , vol.25 , pp. 1565-1579
    • Pirie, N.W.1
  • 46
    • 0035025772 scopus 로고    scopus 로고
    • Effects of genistein isoflavone (4', 5', 7-trihydroxyisoflavone) and dexamethasone on functional characteristics of spermatozoa
    • Kumi-Diaka J, Townsend J. Effects of genistein isoflavone (4', 5', 7-trihydroxyisoflavone) and dexamethasone on functional characteristics of spermatozoa. J Med Food 2001;4:39-47.
    • (2001) J. Med. Food , vol.4 , pp. 39-47
    • Kumi-Diaka, J.1    Townsend, J.2
  • 47
    • 0032211431 scopus 로고    scopus 로고
    • Paradoxical effect of reagents for sulfhydryl and disulfide groups on human sperm capacitation and superoxide production
    • de Lamirande E, Gagnon C. Paradoxical effect of reagents for sulfhydryl and disulfide groups on human sperm capacitation and superoxide production. Free Radic Biol Med 1998;25:803-17.
    • (1998) Free Radic Biol. Med. , vol.25 , pp. 803-817
    • De Lamirande, E.1    Gagnon, C.2
  • 48
    • 3042805170 scopus 로고    scopus 로고
    • Decrease in glutathione content in boar sperm after cryopreservation. Effect of the addition of reduced glutathione to the freezing and thawing extenders
    • Gadea J, Selles E, Marco MA, Coy P, Matas C, et al. Decrease in glutathione content in boar sperm after cryopreservation. Effect of the addition of reduced glutathione to the freezing and thawing extenders. Theriogenology 2004;62:690-701.
    • (2004) Theriogenology , vol.62 , pp. 690-701
    • Gadea, J.1    Selles, E.2    Marco, M.A.3    Coy, P.4    Matas, C.5
  • 50
    • 0029940795 scopus 로고    scopus 로고
    • Spermatid injection into human oocytes. I. Laboratory techniques and special features of zygote development
    • Tesarik J, Mendoza C. Spermatid injection into human oocytes. I. Laboratory techniques and special features of zygote development. Hum Reprod 1996;11:772-9.
    • (1996) Hum. Reprod. , vol.11 , pp. 772-779
    • Tesarik, J.1    Mendoza, C.2
  • 52
    • 0347284250 scopus 로고    scopus 로고
    • Requirement of protein tyrosine kinase and phosphatase activities for human sperm exocytosis
    • Tomes CN, Roggero CM, De Blas G, Saling PM, Mayorga LS. Requirement of protein tyrosine kinase and phosphatase activities for human sperm exocytosis. Dev Biol 2004;265:399-415.
    • (2004) Dev. Biol. , vol.265 , pp. 399-415
    • Tomes, C.N.1    Roggero, C.M.2    De Blas, G.3    Saling, P.M.4    Mayorga, L.S.5
  • 53
    • 0038790009 scopus 로고    scopus 로고
    • A differential mechanism is involved during heparin-and cryopreservation-induced capacitation of bovine spermatozoa
    • Cormier N, Bailey JL. A differential mechanism is involved during heparin-and cryopreservation-induced capacitation of bovine spermatozoa. Biol Reprod 2003;69:177-85.
    • (2003) Biol. Reprod. , vol.69 , pp. 177-185
    • Cormier, N.1    Bailey, J.L.2
  • 54
    • 34548426537 scopus 로고    scopus 로고
    • Effect of genistein on acrosome reaction and zona pellucida binding independent of protein tyrosine kinase inhibition in bull
    • Menzel VA, Hinsch E, Hagele W, Hinsch KD. Effect of genistein on acrosome reaction and zona pellucida binding independent of protein tyrosine kinase inhibition in bull. Asian J Androl 2007;9:650-8.
    • (2007) Asian J. Androl. , vol.9 , pp. 650-658
    • Menzel, V.A.1    Hinsch, E.2    Hagele, W.3    Hinsch, K.D.4
  • 55
    • 0036006880 scopus 로고    scopus 로고
    • Computer assisted semen analyzers in andrology research and veterinary practice
    • Verstegen J, Iguer-Ouada M, Onclin K. Computer assisted semen analyzers in andrology research and veterinary practice. Theriogenology 2002;57:149-79.
    • (2002) Theriogenology , vol.57 , pp. 149-179
    • Verstegen, J.1    Iguer-Ouada, M.2    Onclin, K.3
  • 56
    • 0035802867 scopus 로고    scopus 로고
    • Assessment of sperm quality: A flow cytometric approach
    • Graham JK. Assessment of sperm quality: a flow cytometric approach. Anim Reprod Sci 2001;68:239-47.
    • (2001) Anim. Reprod. Sci. , vol.68 , pp. 239-247
    • Graham, J.K.1


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