Role of cholesterol, calcium, and mitochondrial activity in the susceptibility for cryodamage after a cycle of freezing and thawing

Fertility and Sterility

Volumn 81, Issue 3, 2004, Pages 588-594

  Meseguer, Marcos ^a,b   Garrido, Nicolás ^a,b   Martínez Conejero, Jose Antonio ^c   Simón, Carlos ^a,b,c   Pellicer, Antonio ^a,b,c,d   Remohí, José ^a,b,c  

^a INSTITUTO VALENCIANO DE INFERTILIDAD   (Spain)

^b Fundacion IVI   (Spain)

^c UNIVERSITY OF VALENCIA   (Spain)

^d HOSPITAL UNIVERSITARIO DR PESET   (Spain)

Author keywords

Calcium; Cholesterol; Cryopreservation; Human spermatozoa; Mitochondrial activity

Indexed keywords

CALCIUM ION; CHOLESTEROL;

ARTICLE; CALCIUM CELL LEVEL; CELL MEMBRANE; CLINICAL PROTOCOL; CONTROLLED STUDY; CORRELATION ANALYSIS; CRYOPRESERVATION; CYTOFLUOROMETRY; ENZYME IMMUNOASSAY; HUMAN; HUMAN CELL; MAJOR CLINICAL STUDY; MALE; MALE INFERTILITY; MITOCHONDRION; PRIORITY JOURNAL; SEMEN ANALYSIS; SEMINAL PLASMA; SPERM DONOR; SPERM PRESERVATION; SPERMATOZOON; SPERMATOZOON MOTILITY; STATISTICAL SIGNIFICANCE; THAWING; TREATMENT OUTCOME;

CALCIUM; CELL MEMBRANE; CHOLESTEROL; CRYOPRESERVATION; HUMANS; INTRACELLULAR MEMBRANES; MALE; MITOCHONDRIA; OSMOLAR CONCENTRATION; PROSPECTIVE STUDIES; SEMEN; SEMEN PRESERVATION; SPERMATOZOA;

EID: 1642277862     PISSN: 00150282     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.fertnstert.2003.09.035     Document Type: Article

References (12)

1. Meseguer M., Ruiz A., Garrido N., Zuzuarregui J.L., Reis S., Escudero E., et al. Congelacion y Banco de Semen metodos, organizacion e indicaciones. Remohi J., Pellicer A., Simon C., Navarro J. Reproduccion Humana, 2nd edition. 2002;363-372 McGraw-Hill, Madrid, Spain.
2. Garrido N., Zuzuarregui J.L., Meseguer M., Simon C., Remohi J., Pellicer A. Sperm and oocyte selection and management experience of a 10-year follow-up of more than 2,100 candidates. Hum Reprod. 17:(12):2002;3142-3314.
3. Meseguer M., Garrido N., Remohí J., Pellicer A., Simon C., Martinez-Jabaloyas J.M., et al. Testicular sperm extraction (TESE) and ICSI in patients with permanent azoospermia after chemotherapy. Hum Reprod. 18:2003;1281-1285.
4. +2 uptakes of sperm in relation to cold shock and preservation a review. Reprod Fertil Dev. 5:1993;639-658.
5. Drobnis E.Z., Crowe L.M., Berger T., Anchordoguy T.J., Overstreet J.W., Crowe J.H. Cold shock damage is due to lipid phase transitions in cell membranes a demonstration using sperm as a model. J Exp Zool. 265:1993;432-437.
6. Lin M.H., Morshedi M., Srisombut C., Nassar A., Oehninger S. Plasma membrane integrity of cryopreserved human sperm an investigation of the results of hypoosmotic swelling test, the water test and eosin-Y staining. Fertil Steril. 70:1998;1148-1155.
7. Garner D.L., Thomas C.A., Joerg H.W., DeJarnette J.M., Marshall C.E. Fluorometric assessments of mitochondrial function and viability in cryopreserved bovine spermatozoa. Biol Reprod. 57:1997;1401-1406.
8. World Health Organization. Laboratory manual for the examination of human semen and sperm-cervical mucus interaction. 1999;Cambridge Academic Press, New York.
9. Gil-Salom M., Romero J., Minguez Y., Rubio C., De los Santos M.J., Remohi J., et al. Pregnancies after intracytoplasmic sperm injection with cryopreserved testicular spermatozoa. Hum Reprod. 11:(6):1996;1309-1313.
10. Roeschlau P., Bernt E., Gruber W.A. Enzymatic determination of total cholesterol in serum. Clin Chem Biochem. 12:1974;226.
11. Cerolini S., Maldjian A., Pizzi F., Gliozzi T.M. Changes in sperm quality and lipid composition during cryopreservation of boar semen. Reprod. 121:2001;395-401.
12. Zweig M.H., Campbell G. Receiver-operating characteristic (ROC) plots a fundamental evaluation tool in medicine. Clin Chem. 39:1993;561-577.