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Volumn 22, Issue 3, 1999, Pages 155-162

Effect of freeze-thawing procedure on chromatin stability, morphological alteration and membrane integrity of human spermatozoa in fertile and subfertile men

Author keywords

Chromatin stability; Cryopreservation; Human spermatozoa; Membrane integrity; Morphological alteration

Indexed keywords

ARTICLE; CELL MEMBRANE; CELL SHAPE; CHROMATIN CONDENSATION; CRYOPRESERVATION; FERTILIZATION; HUMAN; HUMAN CELL; MAJOR CLINICAL STUDY; MALE; PRIORITY JOURNAL; SEMEN ANALYSIS; SPERMATOZOON MOTILITY; SPERMATOZOON TAIL; SUBFERTILITY; THAWING;

EID: 0033027767     PISSN: 01056263     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2605.1999.00162.x     Document Type: Article
Times cited : (156)

References (61)
  • 2
    • 0026769066 scopus 로고
    • Reactive oxygen species and human spermatozoa: Analysis of the cellular mechanisms involved in luminol- and lucigenin-dependent chemiluminescence
    • Aitken, R. J., Buckingham, D. W. & West, K. M. (1992) Reactive oxygen species and human spermatozoa: analysis of the cellular mechanisms involved in luminol- and lucigenin-dependent chemiluminescence. Journal of Cell Physiology, 151, 466-477.
    • (1992) Journal of Cell Physiology , vol.151 , pp. 466-477
    • Aitken, R.J.1    Buckingham, D.W.2    West, K.M.3
  • 3
    • 0024365662 scopus 로고
    • Analysis of the relationship between deficit sperm function and the generation of reactive oxygen species in cases of oligozoospermia
    • Aitken, R. J., Clarkson, J. S., Hargreave, T. B., Irvine, D. S. & Wu, F. C. (1989) Analysis of the relationship between deficit sperm function and the generation of reactive oxygen species in cases of oligozoospermia. Journal of Andrology, 10, 214-220.
    • (1989) Journal of Andrology , vol.10 , pp. 214-220
    • Aitken, R.J.1    Clarkson, J.S.2    Hargreave, T.B.3    Irvine, D.S.4    Wu, F.C.5
  • 4
    • 0025633117 scopus 로고
    • Analysis of the relationship between reactive oxygen species production and leukocyte infiltration in fractions of human semen separated on percoll gradients
    • Aitken, R. J. & West, K. M. (1990) Analysis of the relationship between reactive oxygen species production and leukocyte infiltration in fractions of human semen separated on percoll gradients. International Journal of Andrology, 13, 433-51.
    • (1990) International Journal of Andrology , vol.13 , pp. 433-451
    • Aitken, R.J.1    West, K.M.2
  • 5
    • 0024337024 scopus 로고
    • Role of glutathione peroxidase in protecting mammalian spermatozoa from loss of motility caused by spontaneous lipid peroxidation
    • Alvarez J.G. & Storey, B. T. (1989) Role of glutathione peroxidase in protecting mammalian spermatozoa from loss of motility caused by spontaneous lipid peroxidation. Gamete Research, 23, 77-90.
    • (1989) Gamete Research , vol.23 , pp. 77-90
  • 6
    • 0026703651 scopus 로고
    • Evidence of increased lipid peroxidative damage and loss of superoxide dismutase activity as a mode of sub-lethal cryodamage to human sperm during cryopreservation
    • Alvarez, J. G. & Storey, B. T. (1992) Evidence of increased lipid peroxidative damage and loss of superoxide dismutase activity as a mode of sub-lethal cryodamage to human sperm during cryopreservation. Journal of Andrology, 13, 232-241.
    • (1992) Journal of Andrology , vol.13 , pp. 232-241
    • Alvarez, J.G.1    Storey, B.T.2
  • 7
    • 0027237261 scopus 로고
    • Evidence that membrane stress contributes more than lipid peroxidation to sublethal cryodamage in cryopreserved human sperm: Glycerol and other polyols as sole cryoprotectant
    • Alvarez, J. G. & Storey, B. T. (1993) Evidence that membrane stress contributes more than lipid peroxidation to sublethal cryodamage in cryopreserved human sperm: glycerol and other polyols as sole cryoprotectant. Journal of Andrology, 14, 199.
    • (1993) Journal of Andrology , vol.14 , pp. 199
    • Alvarez, J.G.1    Storey, B.T.2
  • 8
    • 0019957290 scopus 로고
    • Zinc and zinc ligands in human seminal plasma II. Contribution by of different origin to the zinc binding properties of human seminal plasma
    • Arver, S. & Eliasson, R. (1982) Zinc and zinc ligands in human seminal plasma II. Contribution by of different origin to the zinc binding properties of human seminal plasma. Acta Physiological Scandinavica, 115, 217-224.
    • (1982) Acta Physiological Scandinavica , vol.115 , pp. 217-224
    • Arver, S.1    Eliasson, R.2
  • 9
    • 0027294843 scopus 로고    scopus 로고
    • Nuclear status of human sperm cells by transmission electron microscopy and image cytometry: Changes in nuclear shape and chromatin structure during spermatogenesis and epididymal transit
    • Auger, J. & Dadoune, J.-P. Nuclear status of human sperm cells by transmission electron microscopy and image cytometry: Changes in nuclear shape and chromatin structure during spermatogenesis and epididymal transit. Biology of Reproduction, 49, 166-175.
    • Biology of Reproduction , vol.49 , pp. 166-175
    • Auger, J.1    Dadoune, J.-P.2
  • 10
    • 0023839404 scopus 로고    scopus 로고
    • Aberrant protamine 1/protamine 2 ratios in sperm of infertile human males
    • Balhorn, R., Reed, S. & Tanphaichitr, N. (1998) Aberrant protamine 1/protamine 2 ratios in sperm of infertile human males. Experientia, 44, 52-55.
    • (1998) Experientia , vol.44 , pp. 52-55
    • Balhorn, R.1    Reed, S.2    Tanphaichitr, N.3
  • 11
    • 0019160510 scopus 로고
    • Morphological characterisation of abnormal human spermatozoa using transmission electron microscopy
    • Bartoov, B., Eltes, F., Weisenberg, R. & Lunenfeld, B. (1980) Morphological characterisation of abnormal human spermatozoa using transmission electron microscopy. Archives of Andrology, 5, 305-322.
    • (1980) Archives of Andrology , vol.5 , pp. 305-322
    • Bartoov, B.1    Eltes, F.2    Weisenberg, R.3    Lunenfeld, B.4
  • 12
    • 0015611906 scopus 로고
    • Variations in the structural character and stability of the nuclear chromatin in morphologically normal human spermatozoa
    • Bedford, J. H., Bent, M. J. Calvin, H. (1973) Variations in the structural character and stability of the nuclear chromatin in morphologically normal human spermatozoa. Journal of Reproduction and Fertility, 333, 19-29.
    • (1973) Journal of Reproduction and Fertility , vol.333 , pp. 19-29
    • Bedford, J.H.1    Bent, M.J.2    Calvin, H.3
  • 13
    • 0025336430 scopus 로고
    • Influence of seminal vesicular fluid on the zinc content of human sperm chromatin
    • Björndahl, L. & Kvist, U. (1990) Influence of seminal vesicular fluid on the zinc content of human sperm chromatin. International Journal of Andrology, 13, 232-237.
    • (1990) International Journal of Andrology , vol.13 , pp. 232-237
    • Björndahl, L.1    Kvist, U.2
  • 14
    • 0026741205 scopus 로고
    • Cryopreservation of human semen: Comparison of cryopreservation, sources of variability, and prediction of post-thaw survival
    • Centola, G. M., Raubertas, R. F. & Mattox, J. H. (1992) Cryopreservation of human semen: comparison of cryopreservation, sources of variability, and prediction of post-thaw survival. Journal of Andrology, 13, 283-288.
    • (1992) Journal of Andrology , vol.13 , pp. 283-288
    • Centola, G.M.1    Raubertas, R.F.2    Mattox, J.H.3
  • 15
  • 16
    • 0022403582 scopus 로고
    • The relationship between the human sperm hypoosmotic swelling, routine semen analysis, and the human sperm zona-free hamster ovum penetration assay
    • Chan, S. Y. W., Fox, E. J., Chan, M. M. C., Tsoi, W., Wang, C., Tang, L. C. H., Tang, G. W. K. & Ho, P. (1985) The relationship between the human sperm hypoosmotic swelling, routine semen analysis, and the human sperm zona-free hamster ovum penetration assay. Fertility and Sterility, 44, 668-672.
    • (1985) Fertility and Sterility , vol.44 , pp. 668-672
    • Chan, S.Y.W.1    Fox, E.J.2    Chan, M.M.C.3    Tsoi, W.4    Wang, C.5    Tang, L.C.H.6    Tang, G.W.K.7    Ho, P.8
  • 17
    • 84907135534 scopus 로고
    • Detrimental effects of cryopreservation on structural and functional integrity of the sperm membrane
    • Check, M. L., Check, J. H. & Long, R. (1991) Detrimental effects of cryopreservation on structural and functional integrity of the sperm membrane. Archives of Andrology, 27, 155-160.
    • (1991) Archives of Andrology , vol.27 , pp. 155-160
    • Check, M.L.1    Check, J.H.2    Long, R.3
  • 18
    • 0023241776 scopus 로고
    • Cryopreservation of human spermatozoa. II Post thaw chronology of motility and of zona-free hamster ova penetration
    • Critser, J. K., Arenson, B. W., Aaker, D. V., Huse-Benda, A. R. & Ball, G. D. (1987) Cryopreservation of human spermatozoa. II Post thaw chronology of motility and of zona-free hamster ova penetration. Fertility and Sterility, 47, 980-984.
    • (1987) Fertility and Sterility , vol.47 , pp. 980-984
    • Critser, J.K.1    Arenson, B.W.2    Aaker, D.V.3    Huse-Benda, A.R.4    Ball, G.D.5
  • 19
    • 0026091906 scopus 로고
    • Effect of cryopreservation on human sperm acrosomes
    • Cross, L. N. & Hanks, S. E. (1991) Effect of cryopreservation on human sperm acrosomes. Human Reproduction, 6, 1279-1283.
    • (1991) Human Reproduction , vol.6 , pp. 1279-1283
    • Cross, L.N.1    Hanks, S.E.2
  • 20
    • 0002230882 scopus 로고
    • Chromatin stability of human spermatozoa in relation to men fertility
    • Eliasson, R. & Enquist, A. M. (1981) Chromatin stability of human spermatozoa in relation to men fertility. International Journal of Andrology, 3 (Suppl.), 73-74.
    • (1981) International Journal of Andrology , vol.3 , Issue.SUPPL. , pp. 73-74
    • Eliasson, R.1    Enquist, A.M.2
  • 21
    • 0013593947 scopus 로고
    • Relationship between sperm quality and chromatin condensation measured by sperm DNA fluorescense using flow cytometry
    • Engh, E., Clausen, O. P., Scholberg, A., Tollesfrud, A. & Puruisk. (1992) Relationship between sperm quality and chromatin condensation measured by sperm DNA fluorescense using flow cytometry. International Journal of Andrology, 14, 282-288.
    • (1992) International Journal of Andrology , vol.14 , pp. 282-288
    • Engh, E.1    Clausen, O.P.2    Scholberg, A.3    Tollesfrud, A.4    Puruisk5
  • 22
    • 0018899119 scopus 로고
    • Comparison of human and mouse sperm chromatin structure by flow cytometry
    • Evenson, D. P., Darzynkiewicz, Z. & Melamed, M. R. (1980) Comparison of human and mouse sperm chromatin structure by flow cytometry. Chromosoma, 78, 225-238.
    • (1980) Chromosoma , vol.78 , pp. 225-238
    • Evenson, D.P.1    Darzynkiewicz, Z.2    Melamed, M.R.3
  • 23
    • 0028723843 scopus 로고
    • Sperm chromatin structure assay: DNA denaturability
    • Evenson, D. P., Jost, L. K., Baer, R. K. et al. (1994) Sperm chromatin structure assay: DNA denaturability Methods in Cell Biology, 42, 159-175.
    • (1994) Methods in Cell Biology , vol.42 , pp. 159-175
    • Evenson, D.P.1    Jost, L.K.2    Baer, R.K.3
  • 24
    • 0015135086 scopus 로고
    • Morphogenetic factors influencing the shape of sperm head
    • Fawcett, D. W., Anderson, W. & Philips, D. M. (1971) Morphogenetic factors influencing the shape of sperm head. Developmental Biology, 26, 220-251.
    • (1971) Developmental Biology , vol.26 , pp. 220-251
    • Fawcett, D.W.1    Anderson, W.2    Philips, D.M.3
  • 25
    • 0023403534 scopus 로고
    • Bovine mucus penetration test and routine semen analysis of fresh and cryopreserved human spermatozoa
    • Gehring, W. G. (1987) Bovine mucus penetration test and routine semen analysis of fresh and cryopreserved human spermatozoa. Andrologia, 19, 544-550.
    • (1987) Andrologia , vol.19 , pp. 544-550
    • Gehring, W.G.1
  • 26
    • 84907128102 scopus 로고
    • Assessment of sperm chromatin condensation: An important test for prediction of IVF-outcome
    • Haidl, G. & Schill, W. B. (1994) Assessment of sperm chromatin condensation: an important test for prediction of IVF-outcome. Archives of Andrology, 32, 263-266.
    • (1994) Archives of Andrology , vol.32 , pp. 263-266
    • Haidl, G.1    Schill, W.B.2
  • 27
    • 0025091810 scopus 로고
    • Cryopreservation of mammalian sperm: What we ask them to survive
    • Hammerstedt, R. H., Graham, J. K. & Nolan, J. P. (1990) Cryopreservation of mammalian sperm: what we ask them to survive. Journal of Andrology, 11, 73-88.
    • (1990) Journal of Andrology , vol.11 , pp. 73-88
    • Hammerstedt, R.H.1    Graham, J.K.2    Nolan, J.P.3
  • 28
    • 0030202411 scopus 로고    scopus 로고
    • A comparison of baseline and induced DNA damage in human spermatozoa from fertile and infertile men, using a modified comet assay
    • Hughes, C. M., Lewis, S. E. M., Mc Kelvey-Martin, V. J. & Thompson W. (1996) A comparison of baseline and induced DNA damage in human spermatozoa from fertile and infertile men, using a modified comet assay. Molecular Human Reproduction, 2, 613-619.
    • (1996) Molecular Human Reproduction , vol.2 , pp. 613-619
    • Hughes, C.M.1    Lewis, S.E.M.2    Mc Kelvey-Martin, V.J.3    Thompson, W.4
  • 29
    • 84907128313 scopus 로고
    • Effect of migration and capacitation on the nuclear stability of human sperm
    • Huret, J. L. & Courtot, A. M. (1984) Effect of migration and capacitation on the nuclear stability of human sperm. Archives of Andrology, 13, 147-152.
    • (1984) Archives of Andrology , vol.13 , pp. 147-152
    • Huret, J.L.1    Courtot, A.M.2
  • 30
    • 0021702629 scopus 로고
    • Nuclear chromatin decondensation abilities of human sperm
    • Huret, J. L. & Miquereau, M. A. (1984) Nuclear chromatin decondensation abilities of human sperm. Archives of Andrology, 12 (Suppl.), 19-22.
    • (1984) Archives of Andrology , vol.12 , Issue.SUPPL. , pp. 19-22
    • Huret, J.L.1    Miquereau, M.A.2
  • 31
    • 0021336185 scopus 로고
    • Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics
    • Jeyendran, R. S., Van der Ven, H. H., Perez-Pelaez, M., Crabo, B. G. & Zaneveld, L. J. (1984) Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. Journal of Reproduction and Fertility, 70, 219-228.
    • (1984) Journal of Reproduction and Fertility , vol.70 , pp. 219-228
    • Jeyendran, R.S.1    Van Der Ven, H.H.2    Perez-Pelaez, M.3    Crabo, B.G.4    Zaneveld, L.J.5
  • 34
    • 0018860475 scopus 로고
    • Importance of spermatozoa zinc as temporary inhibitor of sperm nuclear chromatin decondensation ability in man
    • Kvist, U. (1980) Importance of spermatozoa zinc as temporary inhibitor of sperm nuclear chromatin decondensation ability in man. Acta Physiologica Scandinavica, 109, 79-38.
    • (1980) Acta Physiologica Scandinavica , vol.109 , pp. 79-38
    • Kvist, U.1
  • 35
    • 0023405050 scopus 로고
    • Sperm nuclear zinc, Chromatin stability, and male fertility
    • Kvist, U., Björndahl, L. & Kjellberg, S. (1987) Sperm nuclear zinc, Chromatin stability, and male fertility. Scanning Microscopy, 1, 1241-1247.
    • (1987) Scanning Microscopy , vol.1 , pp. 1241-1247
    • Kvist, U.1    Björndahl, L.2    Kjellberg, S.3
  • 36
    • 0018892031 scopus 로고
    • Influence of seminal plasma on the chromatin stability of ejaculated human spermatozoa
    • Kvist, U. & Eliasson, R. (1980) Influence of seminal plasma on the chromatin stability of ejaculated human spermatozoa. Journal of Andrology, 3, 130-142.
    • (1980) Journal of Andrology , vol.3 , pp. 130-142
    • Kvist, U.1    Eliasson, R.2
  • 37
    • 0031029977 scopus 로고    scopus 로고
    • Comparison of individual antioxidants of sperm and seminal plasma in fertile and infertile men
    • Lewis, S. E., Sterling, E. S., Young, I. S. & Thompson, W. (1997) Comparison of individual antioxidants of sperm and seminal plasma in fertile and infertile men. Fertility and Sterility, 67, 142-147.
    • (1997) Fertility and Sterility , vol.67 , pp. 142-147
    • Lewis, S.E.1    Sterling, E.S.2    Young, I.S.3    Thompson, W.4
  • 38
    • 0026795895 scopus 로고
    • Sperm head ultramorphology and chromatin stability of male with unexplained infertility who fail to fertilise normal human ova
    • Lipitz, S., Bartoov, B., Rajuan, C., Reichart, M., Kedem, P., Mashiach, S. & Dor, J. (1992) Sperm head ultramorphology and chromatin stability of male with unexplained infertility who fail to fertilise normal human ova. Andrologia, 24, 261-269.
    • (1992) Andrologia , vol.24 , pp. 261-269
    • Lipitz, S.1    Bartoov, B.2    Rajuan, C.3    Reichart, M.4    Kedem, P.5    Mashiach, S.6    Dor, J.7
  • 39
    • 0028940712 scopus 로고
    • Under protamination and nicking of DNA in ejaculated human spermatozoa are highly related phenomena
    • Manicardi, G., Bianchi, P. & Pantano, S. (1995) Under protamination and nicking of DNA in ejaculated human spermatozoa are highly related phenomena. Biology of Reproduction, 52, 864-867.
    • (1995) Biology of Reproduction , vol.52 , pp. 864-867
    • Manicardi, G.1    Bianchi, P.2    Pantano, S.3
  • 40
    • 0021987715 scopus 로고
    • Studies on chromosome aberration in the eggs of mice fertilized in vitro after irradiation. I: Chromosome aberrations induced in sperm after X-irradiation
    • Matsuda, Y., Yamada, T. & Tobari, I. (1985) Studies on chromosome aberration in the eggs of mice fertilized in vitro after irradiation. I: Chromosome aberrations induced in sperm after X-irradiation. Mutation Research, 148, 113-117.
    • (1985) Mutation Research , vol.148 , pp. 113-117
    • Matsuda, Y.1    Yamada, T.2    Tobari, I.3
  • 41
    • 0027380165 scopus 로고
    • Effect of cryopreservation on the human sperm acrosome and its response to A23187
    • McLaughlin, E. A., Ford, W. C. L. & Hull, M. G. R. (1993) Effect of cryopreservation on the human sperm acrosome and its response to A23187. Journal of Reproduction and Fertility, 99, 71-76.
    • (1993) Journal of Reproduction and Fertility , vol.99 , pp. 71-76
    • McLaughlin, E.A.1    Ford, W.C.L.2    Hull, M.G.R.3
  • 42
    • 0024306788 scopus 로고
    • Calculation of the incidence of infertility in human populations from sperm measures using the two-distribution model
    • Meistrich, M. L. (1989) Calculation of the incidence of infertility in human populations from sperm measures using the two-distribution model. Progress in Clinical and Biological Research, 302, 275-285.
    • (1989) Progress in Clinical and Biological Research , vol.302 , pp. 275-285
    • Meistrich, M.L.1
  • 43
    • 0013572811 scopus 로고
    • Sperm morphology evaluation
    • (eds R. Menkveld, E. F., Oettle, T. Kruger, R. J. Swanson, A. Acosta, S. Oehninger). Williams & Wilkins
    • Menkveld, R., Oettle, E. E., Kruger, T., Swanson, R. J., Acosta, A. & Oehninger, S. (1991) Sperm morphology evaluation. In: Atlas of Human Sperm Morphology (eds R. Menkveld, E. F., Oettle, T. Kruger, R. J. Swanson, A. Acosta, S. Oehninger) pp.2 & 115. Williams & Wilkins.
    • (1991) Atlas of Human Sperm Morphology , pp. 2
    • Menkveld, R.1    Oettle, E.E.2    Kruger, T.3    Swanson, R.J.4    Acosta, A.5    Oehninger, S.6
  • 44
    • 0025003377 scopus 로고
    • The evaluation of the morphologic characteristics of human spermatozoa according to strict criteria
    • Menkveld, R., Stander, F. S. H., Kotze, T. J. W., Kruger, T. F. & van Zyl, J. A. (1990) The evaluation of the morphologic characteristics of human spermatozoa according to strict criteria. Human Reproduction, 5, 586-592.
    • (1990) Human Reproduction , vol.5 , pp. 586-592
    • Menkveld, R.1    Stander, F.S.H.2    Kotze, T.J.W.3    Kruger, T.F.4    Van Zyl, J.A.5
  • 45
    • 0001934549 scopus 로고
    • Differences in staining with DNA-specific fluorochromes during spermatogenesis
    • Monaco, P. J. & Rasch, E. M. (1982) Differences in staining with DNA-specific fluorochromes during spermatogenesis. Histochemical Journal, 30, 585.
    • (1982) Histochemical Journal , vol.30 , pp. 585
    • Monaco, P.J.1    Rasch, E.M.2
  • 47
    • 0026632842 scopus 로고
    • Sperm plasma membrane integrity in fertile and infertile men
    • Ramirez, J. P., Carreras, A. & Mendoza, C. (1991) Sperm plasma membrane integrity in fertile and infertile men. Andrologia, 24, 141-144.
    • (1991) Andrologia , vol.24 , pp. 141-144
    • Ramirez, J.P.1    Carreras, A.2    Mendoza, C.3
  • 48
    • 0024420213 scopus 로고
    • Lipid perodixation in human spermatozoa as related to midpiece abnormalities and motility
    • Roa, B., Soufir, J. C., Martin, M. & David G. (1989) Lipid perodixation in human spermatozoa as related to midpiece abnormalities and motility. Gamete Research, 24, 127-134.
    • (1989) Gamete Research , vol.24 , pp. 127-134
    • Roa, B.1    Soufir, J.C.2    Martin, M.3    David, G.4
  • 51
    • 0030464440 scopus 로고    scopus 로고
    • Role of reactive oxygen species in male infertility
    • Sharma, R. & Agrawal, A. (1996) Role of reactive oxygen species in male infertility. Urology, 48, 835-850.
    • (1996) Urology , vol.48 , pp. 835-850
    • Sharma, R.1    Agrawal, A.2
  • 52
    • 0022339042 scopus 로고
    • Further evaluation of a simple test for determining the integrity of spermatozoa membranes
    • Spittaler, P. J. & Tylor, J. P. (1985) Further evaluation of a simple test for determining the integrity of spermatozoa membranes. Clinical Reproduction Fertility, 3, 187-196.
    • (1985) Clinical Reproduction Fertility , vol.3 , pp. 187-196
    • Spittaler, P.J.1    Tylor, J.P.2
  • 53
    • 0001979116 scopus 로고
    • Aniline blue staining of human sperm chromatin: Evaluation of nuclear maturation
    • (ed. J. Andre) Martinus Nijhoff Publishers, London
    • Terquem, A. & Dadoune, J. P. (1983) Aniline blue staining of human sperm chromatin: evaluation of nuclear maturation. In: The Sperm Cell (ed. J. Andre), p. 249-252. Martinus Nijhoff Publishers, London.
    • (1983) The Sperm Cell , pp. 249-252
    • Terquem, A.1    Dadoune, J.P.2
  • 54
    • 0026689077 scopus 로고
    • Evaluation of the hypo-osmotic swelling test in relation with advanced methods of semen analysis
    • Van der Saffele, J., Vermeulen, L., Schoonjans, F. & Comhaire, F. H. (1992) Evaluation of the hypo-osmotic swelling test in relation with advanced methods of semen analysis. Andrologia, 24, 213-217.
    • (1992) Andrologia , vol.24 , pp. 213-217
    • Van Der Saffele, J.1    Vermeulen, L.2    Schoonjans, F.3    Comhaire, F.H.4
  • 55
  • 56
    • 0025736290 scopus 로고
    • Immunochemical detection of DNA damage induction and repair at different cellular stage of spermatogenesis of the hamster after in vitro or in vivo exposure to ionising radiation
    • Van Loon, A. A., Den Boer, P. J., Van der Schans, G. P. Mackenbach, P., Grootegoed, A. A., Baan, R. A. & Lohman, P. H. (1991) Immunochemical detection of DNA damage induction and repair at different cellular stage of spermatogenesis of the hamster after in vitro or in vivo exposure to ionising radiation. Experimental Cell Research, 193, 303-309.
    • (1991) Experimental Cell Research , vol.193 , pp. 303-309
    • Van Loon, A.A.1    Den Boer, P.J.2    Van Der Schans, G.P.3    Mackenbach, P.4    Grootegoed, A.A.5    Baan, R.A.6    Lohman, P.H.7
  • 57
    • 0027433496 scopus 로고
    • Effect of freezing method, thawing temperature and post-thaw dilution/washing on motility (CASA) and morphology characteristics of high-quality human sperm
    • Verheyen, G., Pletinex, I. & van Steirtegham, A. (1993) Effect of freezing method, thawing temperature and post-thaw dilution/washing on motility (CASA) and morphology characteristics of high-quality human sperm. Human of Reproduction, 8, 1678-1684.
    • (1993) Human of Reproduction , vol.8 , pp. 1678-1684
    • Verheyen, G.1    Pletinex, I.2    Van Steirtegham, A.3
  • 58
    • 0013571765 scopus 로고
    • Chromatin organisation and the existense of a chromatin-associated DNA-generating system in human sperm
    • (P. O. P. Tso, ed.). 1st edn. Amsterdam: Biomedical press
    • Witkin, S. S., Evenson, D. P. & Bendich, A. (1977) Chromatin organisation and the existense of a chromatin-associated DNA-generating system in human sperm. In: The Molecular Biology of the Mammalian Genetic Apparatus Vol. 1 (P. O. P. Tso, ed.). 1st edn. pp. 345-354. Amsterdam: Biomedical press.
    • (1977) The Molecular Biology of the Mammalian Genetic Apparatus , vol.1 , pp. 345-354
    • Witkin, S.S.1    Evenson, D.P.2    Bendich, A.3
  • 59
    • 0013593949 scopus 로고
    • Sperm storage
    • (eds Grudzinskas & J. L. Youvich) Cambridge University Press, Cambridge.
    • Wolf, D. J. G. (1995) Sperm storage. In: The Spermatozoon (eds Grudzinskas & J. L. Youvich) Cambridge University Press, Cambridge, pp. 192-205.
    • (1995) The Spermatozoon , pp. 192-205
    • Wolf, D.J.G.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.